pNEB-Mos1 7-18-2001 Beverley lab strain B3082

SalI 4486 PflMI 4255 ClaI 4200 SspI 4193 ApoI 232 SacI 438 BclI 490 NruI 599 SacI 3805 XmnI 627 EcoRI 3799 5' IR SphI 638 ApoI 3799 TPASE

NheI 834 ApoI 882

3' IR pNEB-Mos1

4486 bps HindIII 1164

SspI 3218

SapI 1400 XmnI 3011 ampR

ScaI 2894

AlwNI 1934

Trimmed version of Mos1. subcloned from Hartl's original pBluescribe M13+/Mos1 (B3077) into pNEB193. The sequence was confirmed.

LOCUS pNEB-Mos1 4486 bp DNA CIRCULAR SYN 21-NOV-1996 DEFINITION Mos1 with less flanking DNA on the 5' side. B3082 ACCESSION pNEB-Mos1 KEYWORDS . SOURCE Frederico Gueiros-Filho and Stephen M. Beverley FEATURES Location/Qualifiers misc_feature 910..937 /gene="3' IR" /product="inverted repeat 3' to transposase" misc_feature 4138..4165 /gene="5' IR" /product="inverted repeat 5' to transposase" CDS 4309..860 /gene="TPASE" /product="transposase" BASE COUNT 1257 a 1024 c 1085 g 1119 t ORIGIN 1 TCGACGACAA AGAGCACGGA AAACCGCCAA AAAGGTACGA AGACGCCGAA CTGCAAGCAT

B3082 pNEB-Mos1 61 TATTGGATGA AGACGATGCT CAAACGCAAA AACAACTCGC AGAGCAGTTG GAAGTAAGTC 121 AACAAGCAGT TTCCAATCGC TTGCGAGAGA TGGGAAAGAT TCAGAAGGTC GGTAGATGGG 181 TGCCACATGA GTTGAACGAG AGGCAGATGG AGAGGCGCAA AAACACATGC GAAATTTTGC 241 TTTCACGATA CAAAAGGAAG TCGTTTTTGC ATCGTATCGT TACTGGAGAT GAAAAATGGA 301 TCTTTTTTGT TAATCCTAAA CGTAAAAAGT CATACGTTGA TCCTGGACAA CCGGCCACAT 361 CGACTGCTCG ACCGAATCGC TTTGGCAAGA AGACGATGCT CTGTGTTTGG TGGGATCAGA 421 GCGGTGTCAT TTACTATGAG CTCTTGAAAC CCGGCGAAAC GGTGAATACG GCACGCTACC 481 AACAACAATT GATCAATTTG AACCGTGCGC TTCAGAGAAA ACGACCGGAA TATCAAAAAA 541 GACAACACAG GGTCATTTTT CTCCATGACA ACGCTCCATC ACATACGGCA AGAGCGGTTC 601 GCGACACGTT GGAAACACTC AATTGGGAAG TGCTTCCGCA TGCGGCTTAC TCACCAGACC 661 TGGCCCCATC CGATTACCAC CTATTCGCTT CGATGGGACA CGCACTCGCT GAGCAGCGCT 721 TCGATTCTTA CGAAAGTGTG AAAAAATGGC TCGATGAATG GTTCGCCGCA AAAGACGATG 781 AGTTCTACTG GCGTGGAATC CACAAATTGC CCGAGAGATG GGAAAAATGT GTAGCTAGCG 841 ACGGCAAATA CTTTGAATAA ATGATTTTTT CTTTTTCCAC AAAATTTAAC GTGTTTTTTG 901 ATTTAAAAAA AACGACATTT CATACTTGTA CACCTGATAG TTTCTATATT CACCGACTGG 961 AGCCCGTACT GTGGGATGCC TATCTACATC ATTTGGTAGC TTTAAGAAAA AGCCGGATTT 1021 ACCATTTTTA TCCCACGAAA GTGAAGTGCG CATGGTCATT CCTAAGGAAA AGGGAACTGC 1081 TAGCACGCAG KAAAAGCGAG GCATAACAAA CAACCCGAAA GATATTCCAG CAATGGGAAC 1141 GAAATTGATA ATCATAAACC ATAAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG 1201 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA 1261 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT 1321 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA 1381 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 1441 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 1501 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 1561 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 1621 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 1681 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 1741 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 1801 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 1861 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 1921 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 1981 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 2041 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 2101 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 2161 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 2221 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 2281 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 2341 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 2401 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 2461 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 2521 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 2581 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 2641 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 2701 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 2761 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 2821 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 2881 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 2941 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 3001 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 3061 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 3121 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 3181 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 3241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 3301 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG 3361 ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT 3421 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG

B3082 pNEB-Mos1 3481 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC 3541 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA 3601 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG 3661 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA 3721 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT 3781 TGTAAAACGA CGGCCAGTGA ATTCGAGCTC AGGCAAACAT GTTTTGAGTG ACTTCCCTGA 3841 GTCAGATTTA CTTTGGTTAA AAAACAAAGA GCGAACGCAG ACGAGTAAAA CGAAAAAAAA 3901 ACGAAATGTA TGCATGTTGG TTAAAACCAC TTTAAAGTGA TAGTATGGTA AAAAAAAGAA 3961 CCGAGAAAGG TTTCGAATTA CGAGTAAGAA ATGAGCAACT TTTATTTGTA TAACAATATG 4021 ATGTTAGTTT CCTTGTTAAA TGCCAATCGA AGTTCCTTAA TGTTTTAAAG TAATTAATTA 4081 ACACCCACGT ACCTGTGTAT ATATGCGTAA GAACGGGGAC CTAGTTTTAA TTTTTTACCA 4141 GGTGTACAAG TAGGGAATGT CGGTTCGAAC ATATAGATGT CTCGCAAACG TAAATATTTA 4201 TCGATTGTCA TAAAACTTTG ACCTTGTGAA GTGTCAACCT TGACTGTCGA ACCACCATAG 4261 TTTGGCGCGA ATTGAGCGTC ATAATTGTTT ACTCTCAGTG CAGTCAACAT GTCGAGTTTC 4321 GTGCCGAATA AAGAGCAAAC GCGGACAGTA TTAATTTTCT GTTTTCATTT GAAGAAAACA 4381 GCTGCGGAAT CGCACCGAAT GCTTGTTGAA GCCTTTGGCG AACAAGTACC AACTGTGAAA 4441 ACGTGTGAAC GGTGGTTTCA ACGCTTCAAA AGTGGTGATT TTGACG

B3082 pNEB-Mos1