Prelab Dna Isolation

BIO 208 Genetics revised s10

PRE-LAB DNA ISOLATION Answer on separate piece of paper. Type or write neatly. Use your own words. Answers concise and specific.

1. What are these temperatures in Fahrenheit? 0oC 5oC 22oC 37oC 100 oC Use either 9/5C + 32 = F or an online conversion program

2. In degrees C which of the above temperatures: a. is room T b. is refrigerator T c. boils water d. is human body T

3. Define the following terms – use the handout or textbook. if you search online, make sure the definition you provide relates to the biology lab a. Phospholipid bilayer (lipid bilayer) e. Centrifugation b. Cell lysis f. Pellet (after centrifugation) c. Enzyme denaturation g. Supernatant (after centrifugation) d. Histone protein h. High molecular weight DNA

4. What is the role of the following in the DNA isolation procedure? a. Tissue/cell homogenization d. Filtration b. Detergent e. Alcohol (ethanol) c. Protease (meat tenderizer) f. Water

5. Why is it useful to store DNA at 4oC at -20oC (below freezing). Doesn't freezing usually preserve substances?

BIO 208Genetics PRE-LAB DNA ISOLATION revised s10

Answer on separate piece of paper. Type or write neatly. Use your own words. Answers concise and specific.

6. What are these temperatures in Fahrenheit? 0oC 5oC 22oC 37oC 100 oC Use either 9/5C + 32 = F or an online conversion program

7. In degrees C which of the above temperatures: a. is room T b. is refrigerator T c. boils water d. is human body T

8. Define the following terms – use the handout or textbook. if you search online, make sure the definition you provide relates to the biology lab a. Phospholipid bilayer (lipid bilayer) e. Centrifugation b. Cell lysis f. Pellet (after centrifugation) c. Enzyme denaturation g. Supernatant (after centrifugation) d. Histone protein h. High molecular weight DNA

9. What is the role of the following in the DNA isolation procedure? a. Tissue/cell homogenization d. Filtration b. Detergent e. Alcohol (ethanol) c. Protease (meat tenderizer) f. Water

10. Why is it useful to store DNA at 4oC at -20oC (below freezing). Doesn't freezing usually preserve substances?