1 Supplementary data 2 3 4 Streptomyces hokutonensis sp. nov., a novel actinomycete isolated from 5 the strawberry root rhizosphere 6 7 Hideki Yamamura,1 Haruna Ashizawa,1 Moriyuki Hamada,2 Akira Hosoyama,2 8 Hisayuki Komaki2, Misa Otoguro,2 Tomohiko Tamura,2 Yukikazu Hayashi,1 9 Youji Nakagawa,1 Takashi Ohtsuki,1 Nobuyuki Fujita,2 Sadaharu Ui,1 10 and Masayuki Hayakawa1 11 12 13 1 Division of Applied Biological Sciences, Interdisciplinary Graduate School of 14 Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan 15 2 NITE Biological Resource Center (NBRC), National Institute of Technology and 16 Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan 17 18 19 E-mail: [email protected] 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

1 1 37 38 39 40 41 42 43Table S1. Fatty acid content of Streptomyces hokutonensis R1-NS-10T and S. 44prunicolor NBRC 13075T.

Fatty acid (%) S. hokutonensis S. prunicolor 47 R1-NS-10T NBRC 13075T

2OH C11:0 0.5 -

iso-C14:0 6.6 2.5

C14:0 0.9 -

iso-C15:0 7.7 9.9

anteiso-C15:0 24.6 28.4

C15:0 2.7 2.3

iso-C16:1 H 2.6 2.6

iso-C16:0 22.3 20.5

C16:1 cis 9 2.6 1.2

C16:0 9.0 3.4

9-Methyl C16:0 1.6 3.9

anteiso-C17:1 C 2.7 4.7

iso-C17:0 2.1 4.1

anteiso-C17:0 8.1 14.2

C17:1 cis 9 0.6 0.7

Cyclo-C17:0 2.1 0.7

C17:0 0.6 0.5

2 2 48Table S2. Comparison of cultural characteristics of Streptomyces hokutonensis 49strain R1-NS-10T and S. prunicolor NBRC 13075T. +++, abundant growth; ++, 50moderate growth; +, poor growth. 51

S. hokutonensis S. prunicolor Medium R1-NS-10T NBRC 13075T Yeast extract-malt extract agar (ISP 2) Growth +++ + Aerial mycelium Brownish white Grayish white Reverse color Grayish brown Pale yellow Soluble pigment None None Oatmeal agar (ISP 3) Growth +++ + Aerial mycelium Pale brown Grayish white Reverse color Light brown Pale yellow Soluble pigment Yellow None Inorganic salts-starch agar (ISP 4) Growth +++ + Aerial mycelium Light brownish gray Grayish white Reverse color Pale yellowish brown Light brownish gray Soluble pigment None None Glycerol/asparagine agar (ISP 5) Growth +++ +++ Aerial mycelium Pale orange Grayish white Reverse color Reddish brown Reddish brown Soluble pigment Brown None Peptone-yeast extract-iron agar (ISP 6) Growth + + Aerial mycelium None None Reverse color Pale yellow Pale yellow Soluble pigment None None Tyrosine agar (ISP 7) Growth +++ +++ Aerial mycelium Pale orange Grayish white Reverse color Reddish brown Reddish brown Soluble pigment None None Trypticase soy agar (TSA) Growth + + Aerial mycelium None None Reverse color Pale yellow Pale yellow Soluble pigment None None

3 3 52Table S3. Comparison of API ZYM test results for Streptomyces hokutonensis 53strain R1-NS-10T and S. prunicolor NBRC 13075T. +, positive; w, weakly 54positive; , negative. All reported data were obtained in the present study. 55 API ZYM S. hokutonensis S. prunicolor R1-NS-10T NBRC 13075T alkaline phosphatase + + esterase (C4) + + esterase lipase (C8) w w lipase (C14) − − leucine arylamidase + + valine arylamidase + + cystine arylamidase − − trypsin + + α-chymotrypsin − − acid phosphatase + + naphthol-AS-BI-phosphohydrolase + + α-galactosidase + + β-galactosidase + + β-glucuronidase − − α-glucosidase + + β-glucosidase + － N-acetyl-β-glucosaminidase + + α-mannosidase + + α-fucosidase − − 56

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