Table S4 Rvi6 expression in cultivars and genetically modified lines relative to expression in 'Florina' (leaf 1, untreated at time 0) which contains the Rvi6 gene, ± standard deviation to expression in 'Florina' (leaf 1, untreated at time 0)

Relative expression Significance levels a Genotype Conditions of Rvi6 according to figure number:

3 4 5 6 'Florina' untreated 1.000 ± 0.326 D AB

12.978 ± 4.559 AB

20.949 ± 4.277 A decreased temp. 0.689 ± 0.105 0.519 ± 0.083 0.656 ± 0.013 mock (48 h) 1.698 ± 0.368 AB V. inaequalis (48 h) 3.357 ± 1.247 AB mock (96 h) 0.553 ± 0.652 B V. inaequalis (96 h) 3.969 ± 3.770 A 'Priscilla' untreated 0.275 ± 0.186 D B 0.823 ± 0.808 D

12.492 ± 8.674 ABC mock (48 h) 0.213 ± 0.032 B V. inaequalis (48 h) 0.493 ± 0.058 B mock (96 h) 3.086 ± 0.358 AB V. inaequalis (96 h) 1.471 ± 0.045 AB 'Santana' untreated 1.458 ± 0.694 D AB 2.157 ± 1.059 CD 8.428 ± 2.696 BCD decreased temp. 0.286 ± 0.026 1.647 ± 0.905 2.829 ± 1.924 mock (48 h) 0.562 ± 0.446 B V. inaequalis (48 h) 0.923 ± 0.488 AB mock (96 h) 2.131 ± 0.154 AB V. inaequalis (96 h) 1.510 ± 0.212 AB

Ga2-21 untreated 80.360 ± 2.935 B

(CaMV 35S promoter, 107.797 ± 15.358

nos terminator) 118.656 ± 5.457

mock (48 h) 48.141 ± 16.446 BCD

V. inaequalis (48 h) 29.659 ± 6.311 CD

mock (96 h) 60.557 ± 2.385 BC

V. inaequalis (96 h) 150.335 ± 38.269 A

PMdRbcHcrVf2-11/D2 untreated 15.378 ± 0.442 C D

(apple rubisco mock (48 h) 26.849 ± 0.343 CD

gene promoter, V. inaequalis (48 h) 16.966 ± 2.415 D

rubisco-terminator) mock (96 h) 22.687 ± 3.182 D

V. inaequalis (96 h) 36.276 ± 8.535 CD

PMdRbcHcrVf2-11/D4 untreated 28.471 ± 1.508 B CD

(apple rubisco mock (48 h) 36.936 ± 5.681 CD

gene promoter, V. inaequalis (48 h) 18.722 ± 2.063 D

rubisco-terminator) mock (96 h) 17.407 ± 4.324 D

V. inaequalis (96 h) 35.528 ± 3.077 CD C

C7.1.49 untreated 0.316 ± 0.064 D E

(short promotor: 242 bp, 0.663 ± 0.104

native terminator: 0.662 ± 0.163 D

220 bp) mock (48 h) 0.199 ± 0.126 E D

V. inaequalis (48 h) 0.287 ± 0.085 E D

mock (96 h) 0.271 ± 0.027 E A B

V. inaequalis (96 h) 1.439 ± 0.788 C D

C12.1.49 untreated 0.007 ± 0.001 E

(short promoter: 242 bp, 0.019 ± 0.005

native terminator: 0.032 ± 0.004

220 bp) mock (48 h) 0.047 ± 0.024 E

V. inaequalis (48 h) 0.057 ± 0.031 E D mock (96 h) 0.087 ± 0.009 E D V. inaequalis (96 h) 0.133 ± 0.019 E cisSPHcrVf2-11 untreated 1.234 ± 0.035 A B C D A (short promoter: 288 bp, mock (48 h) 1.283 ± 0.212 B B C terminator: sequences V. inaequalis (48 h) 0.414 ± 0.084 D E up to 437 bp) mock (96 h) 2.070 ± 0.971 A A V. inaequalis (96 h) 1.354 ± 0.309 B C 'Gala' b untreated n.d. n.d. n.d. mock (48 h) n.d. V. inaequalis (48 h) n.d. mock (96 h) n.d. V. inaequalis (96 h) n.d. aSignificance levels: samples not connected with the same letter are statistically different (Tukey-Kramer HSD test at P<0.05); b'Gala' was used as negative control lacking Rvi6.