B'gala' Was Used As Negative Control Lacking Rvi6

Table S4 Rvi6 expression in cultivars and genetically modified lines relative to expression in 'Florina' (leaf 1, untreated at time 0) which contains the Rvi6 gene, ± standard deviation to expression in 'Florina' (leaf 1, untreated at time 0)

Relative expression Significance levels a Genotype Conditions of Rvi6 according to figure number:

3 4 5 6 'Florina' untreated 1.000 ± 0.326 D AB

12.978 ± 4.559 AB

20.949 ± 4.277 A decreased temp. 0.689 ± 0.105 0.519 ± 0.083 0.656 ± 0.013 mock (48 h) 1.698 ± 0.368 AB V. inaequalis (48 h) 3.357 ± 1.247 AB mock (96 h) 0.553 ± 0.652 B V. inaequalis (96 h) 3.969 ± 3.770 A 'Priscilla' untreated 0.275 ± 0.186 D B 0.823 ± 0.808 D

12.492 ± 8.674 ABC mock (48 h) 0.213 ± 0.032 B V. inaequalis (48 h) 0.493 ± 0.058 B mock (96 h) 3.086 ± 0.358 AB V. inaequalis (96 h) 1.471 ± 0.045 AB 'Santana' untreated 1.458 ± 0.694 D AB 2.157 ± 1.059 CD 8.428 ± 2.696 BCD decreased temp. 0.286 ± 0.026 1.647 ± 0.905 2.829 ± 1.924 mock (48 h) 0.562 ± 0.446 B V. inaequalis (48 h) 0.923 ± 0.488 AB mock (96 h) 2.131 ± 0.154 AB V. inaequalis (96 h) 1.510 ± 0.212 AB

Ga2-21 untreated 80.360 ± 2.935 B

(CaMV 35S promoter, 107.797 ± 15.358

nos terminator) 118.656 ± 5.457

mock (48 h) 48.141 ± 16.446 BCD

V. inaequalis (48 h) 29.659 ± 6.311 CD

mock (96 h) 60.557 ± 2.385 BC

V. inaequalis (96 h) 150.335 ± 38.269 A

PMdRbcHcrVf2-11/D2 untreated 15.378 ± 0.442 C D

(apple rubisco mock (48 h) 26.849 ± 0.343 CD

gene promoter, V. inaequalis (48 h) 16.966 ± 2.415 D

rubisco-terminator) mock (96 h) 22.687 ± 3.182 D

V. inaequalis (96 h) 36.276 ± 8.535 CD

PMdRbcHcrVf2-11/D4 untreated 28.471 ± 1.508 B CD

(apple rubisco mock (48 h) 36.936 ± 5.681 CD

gene promoter, V. inaequalis (48 h) 18.722 ± 2.063 D

rubisco-terminator) mock (96 h) 17.407 ± 4.324 D

V. inaequalis (96 h) 35.528 ± 3.077 CD C

C7.1.49 untreated 0.316 ± 0.064 D E

(short promotor: 242 bp, 0.663 ± 0.104

native terminator: 0.662 ± 0.163 D

220 bp) mock (48 h) 0.199 ± 0.126 E D

V. inaequalis (48 h) 0.287 ± 0.085 E D

mock (96 h) 0.271 ± 0.027 E A B

V. inaequalis (96 h) 1.439 ± 0.788 C D

C12.1.49 untreated 0.007 ± 0.001 E

(short promoter: 242 bp, 0.019 ± 0.005

native terminator: 0.032 ± 0.004

220 bp) mock (48 h) 0.047 ± 0.024 E

V. inaequalis (48 h) 0.057 ± 0.031 E D mock (96 h) 0.087 ± 0.009 E D V. inaequalis (96 h) 0.133 ± 0.019 E cisSPHcrVf2-11 untreated 1.234 ± 0.035 A B C D A (short promoter: 288 bp, mock (48 h) 1.283 ± 0.212 B B C terminator: sequences V. inaequalis (48 h) 0.414 ± 0.084 D E up to 437 bp) mock (96 h) 2.070 ± 0.971 A A V. inaequalis (96 h) 1.354 ± 0.309 B C 'Gala' b untreated n.d. n.d. n.d. mock (48 h) n.d. V. inaequalis (48 h) n.d. mock (96 h) n.d. V. inaequalis (96 h) n.d. aSignificance levels: samples not connected with the same letter are statistically different (Tukey-Kramer HSD test at P<0.05); b'Gala' was used as negative control lacking Rvi6.