Joshy Francis John

Joshy Francis John 1550 SW 104 Path  Miami, FL 33174  (865) 300-4642.  [email protected]

QUALIFICATIONS Analytical Chemistry  Electrochemistry  Chemical Engineering  Raman & SERS  Nanomaterials Research experience in the synthesis, fabrication and characterization of colloidal nanoparticles and thin film substrates for SERS based sensitive chemical and biological detection. Research background also includes the fabrication of thin film coated electrodes for the electrochemical and SERS monitoring of therapeutic drug release in real time. Excellent work experience in atomic layer deposition, microfluidic fabrication, scanning and electron probe nanomaterial characterization, and electrochemical characterization techniques. Demonstrated leadership in research with strong interpersonal skills and a keen ability to identify new directions in research.

Professional Skills & Expertise  Raman and Surface-Enhanced Raman Spectroscopy (SERS).  Atomic Layer Deposition (ALD).  STEM, AFM, SEM, EDX, PVD, CE, DLS and UV-Vis.  SECM, LSV, DPV, SWV, POT/AMP, CV and other electroanalytical techniques.  Raman & SERS based sensor development.

EDUCATION Doctor of Philosophy in Analytical Chemistry, 2011 THE UNIVERSITY OF TENNESSEE, Knoxville, TN Dissertation: “Thermally Robust ALD and Silver Nanocube based Plasmonic Probe for High Temperature and Microfluidic SERS Measurement” Bachelor of Technology in Chemical & Electrochemical Engineering, 2002 CENTRAL ELECTROCHEMICAL RESEARCH INSTITUTE, India Dissertation: “Synthesis and Characterization of CdSe Nanofilms”

PROFESSIONAL EXPERIENCE

FLORIDA INTERNATIONAL UNIVERSITY, DEPARTMENT OF BIOMEDICAL ENGINEERING, Miami, FL Postdoctoral Associate, 2011-Present  Synthesized colloidal silver and gold nanosphere from the oil/water based microemulsion system for label free SERS based detection of stress proteins.  Presently working on the analytical quantification of stress proteins, nanoparticle delivery into yeast cells and in- vivo SERS. In parallel working on the multiplexed detection of stress proteins and isolation of the signal of interest in the intracellular environment using chemometrics method.

UNIVERSITY OF TENNESSEE, DEPARTMENT OF CHEMISTRY, Knoxville, TN Graduate Assistant, 2005-2011  Produced microfluidic based SERS substrate using soft lithographic technique from poly(dimethylsiloxane) as device platform. Achieved greater reproducibility of 6%.  Fabricated gold film coated platinum electrode and monitored in real time the release of dacarbazine from the nanoemulsion using both electrochemical and SERS technique.  Synthesized silver nanocube and spherical gold nanoparticles, and performed Raman and SERS interpretation of organic molecules.  Fabricated and characterized K+ ion selective micropipette electrode employing scanning electrochemical microscopy (SECM) technique. JOSHY FRANCIS JOHN PAGE 2 PROFESSIONAL EXPERIENCE

OAK RIDGE NATIONAL LABORATORY, Oak Ridge, TN Research Assistant, 2008-2010  Successfully developed and optimized ultrathin Alumina coated Au/Ag multilayer metal film to elevate SERS sensitivity and thermal stability of the substrate at higher temperature. Attained 10,000-fold in signal enhancement.  Fabricated sub-3nm thick conformal layer of Alumina on a silver island film, utilizing atomic layer deposition (ALD) and enhanced stability of the substrate against oxidation and higher temperature.  Evaluated interfacial changes accompanying the phase transformation of calcium nitrate tetrahydrate in-situ wise by SERS as a function of temperature.

BPL SOFT ENERGY SYSTEM, India Senior R&D Engineer, 2002-2005  Maximized battery cell discharge capacity and efficiency to 74% by synthesis and characterization (FTIR, AAS, and particle size and surface area analysis) of novel lithium cathode material.  Supervised R&D activities, partnered with production and quality assurance department to rectify material defects, elevate performance, and lower costs.

SUNDRAM FASTENERS LIMITED, India Summer Internship, 5/2000-7/2000  Formulated an electrolyte bath composition for the electroless plating of nickel to achieve improved quality of coating using Box-Behnken design, resulting in greater reflectivity and hardness.

CENTRAL ELECTROCHEMICAL RESEARCH INSTITUTE, India Undergraduate Student, 1998-2002  Synthesized and performed surface level characterization and bandgap measurements of Cadmium Selenide nanofilms.

INVENTION DISCLOSURE

 S. Dai, S. Mahurin, J. F. John. “Stable & Reusable SERS Substrate”, Oak Ridge National Laboratory, TN, USA.

PUBLICATIONS & PRESENTATIONS

 J.F. John, V. Bhardwaj, S. Srinivasan, A. J. McGoron. “Sensitive and Label Free Raman Detection and the Yeast Cellular Uptake of Colloidal Silver Nanosphere for In-vivo SERS Quantification of Caspase-3 Proteins”. (Nanoflorida 2011 Conference – Accepted)  S. Mahurin, J. F. John, S. Dai, M. J. Sepaniak. “A Reusable SERS Substrate fabricated by Al2O3-ALD on a Multi- Layer Silver and Gold Film.” (Journal of Applied Spectroscopy)  J. F. John, S. Mahurin, S. Dai, M. J. Sepaniak. “Towards the Development of a Rationally Designed Surface Architecture SERS Platform using Atomic Layer Deposition.” (Pittcon Conference 2010)  J. F. John, S. Mahurin, S. Dai, M. J. Sepaniak. “Use of Atomic Layer Deposition to Improve the Stability of Silver Substrates for In-Situ, High Temperature SERS Measurements.” Journal of Raman Spectroscopy (2009), 41(1), 4-11.  N. Abu-Hatab, J. F. John, J. M. Oran, M. J. Sepaniak. “Multiplexed Microfluidic Surface-Enhanced Raman Spectroscopy.” Applied Spectroscopy (2007), 61(10), 1116-1122.  M. J. Sepaniak, N. Abu-Hatab, A. Wellman, J. F. John, M. Connatser. “Chemical and Biochemical Analysis Using Microfluidics-Localized Field Platforms.” Proceedings of SPIE-The International Society for Optical Engineering (2007), 6759 (Smart Biomedical and Physiological Sensor Technology V), 675902/1-675902/10.

AFFILIATIONS American Chemical Society  SPIE-International Society for Optical Engineering  Society for Applied Spectroscopy