PRODUCT: BUCKPLUS-LAP PRODUCT No: BKP-LAP Dosage: 0.5 Ml

 Fixed timed inseminations: Breed 36 hrs after progestin withdrawal.

4. Synchronized with progestin implant (CIDR®, pessary sponge, Synchro-Mate B®) + CERVINE semen sexing agent - MALE prostaglandins (LUTALYSE® or ESTRUMATE®):  Fixed timed insemination: Breed 68 hrs after progestin withdrawal.

5. Synchronized heats and natural heats: PRODUCT: BUCKPLUS-LAP PRODUCT No: BKP-LAP Dosage: 0.5 ml  Estrus detection: Breed 24 hours after marking by vasectomized male. INDICATIONS For increasing the percentage of MALE fawns in deer and elk. For use with laparoscopic artificial insemination (LAI) of fresh or frozen Cervine semen. STORAGE CONDITIONS Store lyophilized product in freezer (-20oC). Avoid moisture and strong sunlight. DOSAGE Do not reuse or store after rehydration. Each vial of BUCKPLUS-LAP contains 2 units of the deer sexing agent BUCKPLUS and will sex up to 0.5 ml of semen (1 unit per 0.25 ml). HOW SUPPLIED BUCKPLUS is lyophilized in the following package sizes: 0.25 ml, 0.50 ml, 1ml, and 20 unit vials.  Recommended dilution rate = 0.50 ml  Recommended extension titer = 50-100 million live sperm/ml. WARNINGS  Recommended insemination volume = 0.25 or 0.50 ml. KEEP OUT OF REACH OF CHILDREN.  Recommended insemination dose = 25 million live sperm.

Manufactured by: NOTICE BUCKPLUS is a non-prescription biopharmaceutical agent. US Federal law does not require that this product be used by or on the order of a licensed veterinarian. Please consult local regulations. EMLAB GENETICS, LLC, Arcola, IL 61910 USA

DESCRIPTION BUCKPLUS is a spermagenic sexing agent specifically formulated for artificial insemination of deer. For more information, please call 708-442-3964 or log on to www.emlabgenetics.com Each vial contains an amount of lyophilized BUCKPLUS necessary to sex a specific volume of semen. The agent is activated by the addition of semen + extender directly to the vial. After a short incubation, the semen is ready for artificial insemination. BUCKPLUS™ is a trademark of EMLAB GENETICS, LLC MODE OF ACTION BUCKPLUS works by enhancing the fertility of the Y-chromosome bearing (male) sperm while reducing the fertility of the X-chromosome bearing (female) sperm. When inseminated at or near EMLAB GENETICS © 2015 ovulation, most ova are fertilized by the Y-chromosome bearing (male) sperm. The MALE SEX REV 10.05.15 RATIO is increased by 20-30% (Ave. 75%) and conception is boosted 5-25%.

PREPARATION OF SEXED SEMEN

FRESH  Collect fresh semen and dilute 1:1 with a suitable deer semen extender.  Determine semen parameters. Note: It is necessary to assess semen parameters before mixing with BUCKPLUS.  Extend semen to 50 million live sperm per ml. See TABLE 1.  Equilibrate extended semen to 95-98.6oF (35-37oC).  Warm the BUCKPLUS vial to 95-98.6oF (35-37oC) for 2 minutes.  Add 1.0 ml of extended semen directly to the BUCKPLUS vial. Reseal and gently mix.  IMPORTANT: Incubate the semen + BUCKPLUS in a water bath or incubator for 15 minutes at 95-98.6oF (35-37o) prior to insemination. Do not exceed 60 minutes.  Load insemination pipette and inseminate as usual.

FROZEN  Warm the BUCKPLUS vial to 95-98.6oF (35-37oC) for 2 minutes.  Thaw semen as usual.  Add a total of 1.0 ml of frozen thawed semen directly to the BUCKPLUS vial.  Reseal the vial and gently mix.  IMPORTANT: Incubate the semen + BUCKPLUS in a water bath or incubator for 15 minutes at 95-98.6oF (35-37o) prior to insemination. Do not exceed 60 minutes.  Load insemination pipette and inseminate as usual.

Table 1 Addition of deer semen to BUCKPLUS.

Volume of Extension No. of live No. of AI AI Dose (ml) extended titer (live sperm per Doses semen (ml) sperm/ ml) insemination

0.25 0.50 100 x 106 2 25 x 106

0.50 0.50 50 x 106 1 25 x 106

Note: Note: Do not add more than the recommended volume of semen. When “splitting straws”, mix and incubate as with a single straw. For LAP insemination, incubate in the vial and withdraw separate doses as needed.

TIMING OF INSEMINATION Variations in synchronization protocols, superovulation regimens and species differences will give rise to variations in the time of onset of heat and time of ovulation. These variances are important when using fixed-time breeding. For fixed-timed inseminations, please consult the following recommendations. Please note: The following guidelines for fixed-time AI may need adjustment according to Cervine breed, species, or breeding protocol. Please consult the inseminator or local veterinarian. In general, apply BUCKPLUS at or near the time of ovulation (24 hours after onset of heat).

1. Synchronized with progestin implant (CIDR®, pessary sponge, Synchro-Mate B®):  Fixed-timed inseminations: Breed 60-64 hours after progestin withdrawal.

2. Synchronized with progestin implant (CIDR®, pessary sponge, Synchro-Mate B®) + PMSG (Folligon®):  Fixed-timed inseminations: Breed 54-58 hrs after progestin withdrawal.

3. Super-ovulated with progestin implant (CIDR®, pessary sponge, Synchro-Mate B®) + FSH (Pluset®, Follitropin-V®):