1 Microdialysis in Both Exercised and Non-Exercised Arm 2 H After the First Bout of Exercise

Suppl. Table 4 Individual scintigraphy results 6 and 20 hours after the first and the second bout of eccentric exercise. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The accumulation of radioactivity was calculated as a ratio between a region of interest (ROI) in the exercised arm and radioactivity in the same ROI in the control arm, corrected for background radiation.

Time after eccentric exercise SUBJECT first bout second bout 6h 20h 6h 20h CEL S05 (m) 1.34 1.30 1.04 1.07 CEL S07 (f) 1.35 1.58 * * CEL S15 (m) 1.11 1.17 1.37 1.36 PBO S04 (m) 1.30 1.37 * * PBO S12 (m) 1.23 1.17 * * PBO S28 (f) 1.35 1.46 1.49 1.52 CEL S01(m) 1.16 1.74 1.28 1.38 CEL S10 (m) 1.23 1.34 1.12 1.06 PBO S02 (m)2 1.42 2.28 1.41 1.31 PBO S06 (f) 1.92 3.40 1.45 2.20 PBO S08 (m) 2.09 1.98 * * PBO S14 (m)1 1.04 1.17 * * PBO S16 (f) 1.31 1.32 * * PBO S18 (m) * * * * PBO S25 (m) 1.30 1.50 * * PBO S27 (f) 1.36 1.37 1.28 1.14 CEL S03 (m) 1.25 1.79 * *

CEL S11 (f)2 2.20 3.86 1.78 2.56 CEL S17 (m) 1.46 2.00 1.74 1.76 CEL S22 (m) * * * * CEL S26 (f)2 2.14 3.27 * * PBO S13 (m)1 1.48 2.36 2.12 2.10 PBO S29 (m) 1.91 2.53 1.98 1.68 1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise. 2 1000% higher radioactivity compared same area in the contralateral arm was observed in small area of the mid part of m. biceps brachii at 20 h after the first exercise bout * Subject didn’t participate to the measurements f female, m male Suppl. Table 5. Number of CD66b positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cell indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cell indicates deformation in the skeletal muscle fibre structure.

Time after the first bout of eccentric exercise second bout SUBJECT 1h 2d 4d 7d 1h/2d ex cont ex cont ex cont ex cont ex cont CEL S05 (m) 0 0 0 5 0 0 (127) (96) (126) (147) § (142)a (86)a CEL S07 (f) 0 0 0 0 0 0 (354) (528) (187) (371) (184)a (194)a CEL S15 (m) * 0 0 0 0 0 (261) (240) (256) (259)b (182)b PBO S04 (m) 0 0 (280) (121) PBO S12 (m) 0 0 0 * 0 0 (155) (277) (90) (161)a (74)a PBO S28 (f) 0 0 0 0 0 0 (255) (266) (147) (291) (181)b (275)b CEL S01 (m) 3 0 2 0 0 0 (106) # (232) (131) # (93) (154)b (159)b CEL S10 (m) 0 0 0 0 0 1 (110) (166) (247) (184) (248)a (125)a # PBO S02 (m) 0 3 0 * 0 0 (140) (146) § (56) (46)b (55)b PBO S06 (f) 2 0 0 4 1 1 (65) # (115) (345) (94) # (294)b # (238)b # PBO S08 (m) 0 0 0 0 0 2 (212) (194) (187) (168) (157)a (49)a # PBO S14 (m)1 1 5 0 0 1 0 (238) # (255) § (185) (295) (335)b # (173)b PBO S16 (f) 1 0 3 0 0 0 (194) § (224) (135) § (205) (145)a (191)a PBO S18 (m) 0 0 30 20 0 0 (186) (178) (184) § (188) § (182)a (168)a PBO S25 (m) 0 0 2 0 3 0 (135) (219) (58) # (169) (106)b # (176)b PBO S27 (f) 0 * 0 0 0 1 (241) (195) (199) (235)b (207)b # CEL S03 (m) 0 0 * 0 (165) (54) (63) CEL S11 (f) 0 0 1 0 0 0 (346) (289) (434) § (369) (257)b (339)b CEL S17(m) 2 0 1 0 2 2 (187) # (98) (266) # (174) (258)a # (245)a # CEL S22 (m) 2 4 (100) § (100) # CEL S26 (f) 0 1 0 0 1 1 (295) (229 )# (148) (292) (207)b § (247)b § PBO S13 (m)1 0 0 0 * 0 0 (227) (345) (205) (362)b (260)b PBO S29 (m) 4 1 0 1 0 1 (118) # (213) # (267) (204) # (257)a (222)a # 1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise. a 1h and b 2 d after the second bout of exercise. * Counting of CD66b positive cells was not possible due to bad quality of stained sections. # CD66b positive cells were located in inside capillaries or vessels, or in blood clots § CD66b positive cells were located in the endomysium or inside damaged fibres. f female, m male Suppl. Table 6 Number of CD16 positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cells of the table indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cells of the table indicates deformation in the skeletal muscle fibre structure.

Time after the first bout of eccentric exercise second bout SUBJECT 1h 2d 4d 7d 1h/2d ex cont ex cont ex cont ex cont ex cont CEL S05 (m) 4 2 26 317 11 8 (139) (96) (115) (103) (106)a (84)a CEL S07 (f) 5 2 2 23 4 4 (254) (415) (136) (270) (108)a (121)a CEL S15 (m) * 6 6 4 37 15 (205) (216) (221) (175)b (123)b PBO S04 (m) 18 6 (208) (84) PBO S12 (m) 3 4 8 * 1 2 (100) (257) (59) (114)a (54)a PBO S28 (f) 5 5 11 17 16 3 (244) (179) (107) (116) (124)b (214)b CEL S01 (m) 22 15 51 57 23 2 (59) (151) (98) (89) (86)b (119)b CEL S10 (m) 1 1 54 4 3 4 (110) (140) (175) (168) (233)a (123)a PBO S02 (m) 29 4 109 7 87 12 (125) (122) (35) (125) (23)b (41)b PBO S06 (f) 32 1 70 17 71 3 (38) (93) (266) (87) (282)b (203)b PBO S08 (m) 9 10 100 118 27 2 (136) (101) (128) (119) (101)a (49)a PBO S14 (m)1 12 77 20 3 14 3 (129) (195) (104) (159) (242)b (166)b PBO S16 (f) 7 7 232 2 2 2 (137) (151) (92) (173) (129)a (134)a PBO S18 (m) 1 4 226 239 22 1 (160) (135) (84) (83) (68)a (79)a PBO S25 (m) 5 7 4 1 4 3 (56) (157) (113) (84) (55)b (64)b PBO S27 (f) 22 * 626 1 33 11 (194) (195) (179) (159)b (132)b CEL S03 (m) 23 3 * 7 (94) (58) (45) CEL S11 (f) 25 2 109 16 36 19 (260) (209) (356) (244) (197)b (151)b CEL S17 (m) 6 0 22 2 81 0 (122) (63) (179) (125) (158)a (168)a CEL S22 (m) 89 2 (204) (192) CEL S26 (f) 54 4 32 25 70 31 (227) (232) (183) (162) (97)b (177)b PBO S13 (m)1 8 1 713 * 72 4 (227) (345) (205) (362)b (260)b PBO S29 (m) 0 1 1 12 96 0 (72) (145) (193) (94) (151)a (140)a 1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise. a 1h and b 2 d after the second bout of exercise. * Counting of CD16 positive cells was not possible due to bad quality of stained sections. f female, m male Suppl. Table 7 Number of CD68 positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cells of the table indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cells of the table indicates deformation in the skeletal muscle fibre structure.

Time after the first bout of eccentric exercise second bout SUBJECT 1h 2d 4d 7d 1h/2d ex cont ex cont ex cont ex cont ex cont CEL S05 (m) 21 10 19 108 14 31 (131) (84) (144) (138) (203)a (68)a CEL S07 (f) 7 11 17 23 15 17 (216) (551) (188) (349) (143)a (133)a CEL S15 (m) * 9 29 15 48 33 (213) (115) (185) (145)b (153)b PBO S04 (m) 38 19 (203) (64) PBO S12 (m) 13 20 20 * 31 28 (116) (261) (59) (127)a (64)a PBO S28 (f) 23 16 16 12 46 11 (224) (189) (99) (186) (158)b (193)b CEL S01(m) 61 28 83 70 29 13 (66) (180) (90) (84) (106)b (92)b CEL S10 (m) 12 12 95 5 14 20 (118) (147) (212) (198) (233)a (116)a PBO S02 (m) 53 32 148 49 57 10 (138) (121) (27) (65) (28)b (58)b PBO S06 (f) 51 22 100 20 75 20 (53) (97) (105) (96) (251)b (167)b PBO S08 (m) 12 23 61 83 33 10 (130) (115) (150) (157) (153)a (78)a PBO S14 (m)1 40 49 19 21 63 72 (233) (167) (111) (185) (284)b (152)b PBO S16 (f) 25 19 263 18 66 42 (62) (123) (72) (102) (102)a (130)a PBO S18 (m) 17 11 124 142 106 19 (147) (129) (119) (118) (107)a (119)a PBO S25 (m) 81 24 43 34 * 28 (96) (117) (111) (116) (92)b PBO S27 (f) 52 * 968 15 45 21 (157) (184) (144) (165)b (140b CEL S03 (m) 70 45 * 18 (74) (98) (40) CEL S11 (f) 26 11 73 18 50 35 (268) (337) (454) (245) (244)b (313)b CEL S17 (m) 38 20 32 18 75 10 (147) (88) (191) (170) (242)a (258)a CEL S22 (m) 204 26 (153) (120) CEL S26 (f) 51 27 54 41 66 67 (265) (247) (180) (148) (90)b (171)b PBO S13 (m)1 49 5 932 103 156 43 (187) (153) (196) (87) (300)b (184)b PBO S29 (m) 24 10 16 40 155 9 (202) (206) (251) (167) (217)a (181)a 1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise. a 1h and b 2 d after the second bout of exercise. * Counting of CD68 positive cells was not possible due to bad quality of stained sections. f female, m male