College of Engineering and Science Updated: November, 2012

RESUME Frank Alexis, Ph.D.

PERSONAL DATA Assistant Professor Department of Bioengineering 203 Rhodes Engineering Research Center Clemson University Clemson, SC 29634 864-656-5003

October 09, 1976 Pointe-Noire Congo France

EDUCATION Ph.D., Nanyang Technological University, 2005, Materials Science Engineering.

M.S., Technological University of Montpellier, 2000, Materials Science and Interfaces.

B.S., Technological University of Montpellier, 1999, Chemistry.

PROFESSIONAL EXPERIENCE Clemson University, 2009-, Assistant Professor of Bioengineering

Massachusetts Institute of Technology, 2006-2009, Research Associate, Department of Health Science and Technology.

Brigham and Women’s Hospital, 2006-2009, PostdocFellow, Laboratory of Biomaterials and Nanomedicine.

CONSULTING EXPERIENCE

Stericoat Inc., Cambridge, Massachusetts (2008), assisted for the development of novel implant coatings.

MEMBERSHIPS Member, Society For Biomaterials, SFB (2009- )

1 Member, American Chemical Society, ACS (2008- )

Member, Society of Chemical Industry, SCI (2004- )

PROFESSIONAL ACTIVITIES Singapore A*Star Government Scholarship, Assessor (2004)

Singapore Science and Engineering Fair, Judge (2003)

Encyclopedia of Biomaterials and Biomedical Engineering, G. E Wneck and G.L Bowlin. Marcel Dekker, NY, Reviewer (2005).

ASM International, Reviewer (2006)

Molecular Pharmaceutics, Reviewer (2008- )

Biomacromolecules, Reviewer (2008- )

Cancer Epidemiology, Reviewer (2008- )

Cancer Research, Reviewer (2008- )

Nano Science and Technology Institute Conference, Reviewer (2008- ).

Molecular Cancer Therapeutics, Reviewer (2009- )

Bioconjugates Chemistry, Reviewer (2009- )

Cancer Epidemiology, Reviewer (2009- )

Journal of Biomedical Materials Research: Part A, Reviewer (2009- )

Journal of Biomaterial Science: Polymer Edition, Reviewer (2009- )

US Army Medical Research and Materiel Command (USAMRMC) Grant, Reviewer (2009- )

Ocular Pharmacology and Therapeutics, Reviewer (2010- )

Royal Society for Chemistry, Reviewer (2010- )

Therapeutic Delivery, Reviewer (2010- )

Trends in Molecular Medicine, Reviewer (2010- )

2 Advanced Functional Materials, Reviewer (2010-)

Chemical Papers, Reviewer (2010-)

Chemistry an Asian Journal, Reviewer (2010-)

ORAU Grant, Reviewer (2010- )

Biomedical Engineering Society Conference, Reviewer (2011-)

ChemPhysChem, Reviewer (2011-)

Journal of Biomedical Materials Research: Part B, Reviewer (2011- )

European Journal of Pharmaceutics and Biopharmaceutics, Reviewer (2011- )

Advanced Materials, Reviewer (2011-)

National Conference for Undergraduate Research, Biomedical Engineering Society Conference, Reviewer (2011-)

Nanomedicine, Reviewer (2012-)

Small, Reviewer (2012-)

Acta Biomateriala, Reviewer (2012-)

Langmuir, Reviewer (2012-)

Expert Opinion in Drug Delivery, Reviewer (2012-)

Journal of Nanotechnology, Reviewer (2012-)

Journal of Visualized Experiments, Reviewer (2012-)

NSF-MRI Grant, Reviewer (2012-)

Singapore-MIT Alliance for Research and Technology Innovation Grant, Reviewer (2012-)

Fonds recherche Québec - Nature et technologies (FRQNT), Reviewer (2012-)

Clemson’s Biomaterials Day-Society For Biomaterials, Organizer (2012)

3 Materials, Reviewer (2012-)

International Organizing Committee of the IUPAC MacroMolecular Complexes Conference, MMC-15:2013, Committee Member (2012)

Polymer Degradation and Stability, Reviewer (2012-)

American Association for the Advancement of Science (AAAS), INDO- US SCIENCE & TECHNOLOGY Grant, Reviewer (2012).

Military Medicine, Reviewer (2012-)

PUBLICATIONS Book Chapter Kolishetti, N., Alexis, F., Pridgen, E., Farokhzad, O. C., Nanoplatform-Based Molecular Imaging, 1st edition (2011), John Wiley and Sons, Inc., Hoboken, NJ.

Rosen, Y., Mattix, Rao A., Alexis, F. Nanomedicine in Health and Disease, 1st edition (2011), Taylor and Francis Group, LLC, Boca Raton, FL.

Moore, T., Graham, E., Mattix, B., Alexis, F., Biomaterials Science: An Integrated Clinical and Engineering Approach, 1st edition (2012), Taylor and Francis Group, LLC, Boca Raton, FL.

Venkatraman, S.; Keith, K.; Rosen, Y.; Huang, Y.; Steele, T.W.J.; Alexis, F., Polymers for Vascular and Urogenital Applications, 1st edition (2012), Taylor and Francis Group, LLC, Boca Raton, FL.

Prior to Clemson: Alexis, F.; Zeng, J.; Wang, S., Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual, 1st edition (2007), Cold Spring Harbor Laboratory Press, New York, NY.

Pridgen, E.; Alexis, F.; Farokhzad, O. C.; Langer, R., Methods in Bioengineering Book Series Nanoscale Bioengineering and Nanomedicine, 1st edition (2009), Artech House , Boston, MA.

Alexis, F.; Pridgen, E.; Langer, R.; Farokhzad, O. C., Handbook of Experimental Pharmacology 197, 1st edition (2010), Springer, Berlin, Germany.

Referred Journal Publications Loo, S.C.J., Moore, T., Banik, B., Alexis, F., “Biomedical Applications of Hydroxyapatite Nanoparticles”, Current Pharmaceutical Biotechnology 11, 333-342 (2010).

Zha, L., Banik, B., Alexis, F., “Stimuli Responsive Nanogels”, Soft Matter 7, 5908-5916 (2011).

4 Stone, R., Willi, T.J., Mefford, T., Rosen, Y., Alexis, F., “Targeted Magnetic Hyperthermia”, Therapeutic Delivery 2, 815-838 (2011).

Banik, B., Alexis, F., “Spatial Functionalization of Polymeric Films”, Cover page of Biomaterials Forum 33, 3, (2011).

Xiao, Z., Levy-Nissenbaum, E., Alexis, F., Lupták, A., Teply, B.A., Chan, J.M., Shi, J., Digga, E., Cheng, J., Langer, R., Farokhzad, O.C., “Engineering of Targeted Nanoparticles for Cancer Therapy Using Internalizing Aptamers Isolated by Cell-Uptake Selection”, ACS Nano 6, 696-704 (2012).

Chen, H., Colvin, D. C., Qi, B., Moore, T., He, J., Mefford, O. T., Alexis, F., Gore, J. C., Anker, J. N., “Magnetic and optical properties of multifunctional core-shell radioluminescence nanoparticles”, J. Mat. Chem. 22, 12802-12809 (2012).

Chen, H, Moore, T., Qi, B., Colvin, D.C., Jelen, E.K., He, J., Mefford, T.O., Gore, J.C., Alexis, F., Anker, J.N., “Radioluminescent nanocapsules with pH-triggered doxorubicin release and radioluminescence monitoring of release”, ACS Nano, Accepted (2012)

Podila, R., Moore, T., Rao A., Alexis, F. “Graphene Coatings for Biomedical Implants”, RCS Advances, Accepted (2012).

Podila, R.; Moore, T.; Alexis, F.; Rao, A.M., “Graphene Coatings for Biomedical Implants”, JOVE, Accepted (2012).

Prior to Clemson: Alexis, F., Rath, S. K., Boey, F.Y.C., Venkatraman, S.,"Study of the Initial Stages of Drug Release from a Degradable Matrix of Poly (d,l-lactide-co- glycolide)", Biomaterials 25, 813-821 (2004).

Alexis, F., Rath, S. K, Venkatraman, S., “In Vitro Study of Release Mechanisms of Paclitaxel and Rapamycin from Drug-Incorporated Biodegradable Stent”, Journal of Controlled Release 98, 67-74 (2004).

Alexis, F., Rath, S. K., Boey, F.Y.C., Venkatraman, S., “Controlled drug release of bioerodible polymers: effect of drug type and composition”, Journal of Controlled Release 102, 333-344 (2005).

Alexis, F., "Factors Affecting Degradation and Drug Release Mechanisms of Bioerodible Polymers", Polymer International 54, 36-46 (2005).

Tang, G.P., Zeng, S., Alexis, F., Yang, Y.Y., Wang, S., “Low Molecular Weight Polyethylenimine Copolymerized with B-cyclodextrin for Gene Delivery”, Journal of Gene Medicine 8, 736-744 (2006).

5 Alexis, F., Rath, S. K., Gan, L-H., Venkatraman, S.,”Some Insight into Hydrolytic Scissions Mechanisms in Bioerodible Polyesters”, Journal of Applied Polymer Science 102, 3111-3117 (2006).

Alexis, F., Lo, S.L., Wang, S., “Covalent Attachment of Low Molecular Weight Polyethylenimine Improves Tat-Peptide Mediated Gene Delivery", Advanced Materials 18, 2174-2178 (2006).

Zhang, L., Radovic-Moreno, A., Alexis, F., Gu, F.X., Basto, P., Langer, R, Farokhzad, O. C., “Co-Delivery of Hydrophobic and Hydrophilic Drugs from Nanoparticle-Aptamer Bioconjugates”, ChemMedChem. 2, 1268-1271 (2007).

Gu, F.X., Karnik, R., Wang, A. Z., Alexis, F., Levy-Nissenbaum, E., Hong, S., Langer, R., Farokhzad, O. C.,” Targeted Nanoparticles for Cancer Therapy”, NanoToday 2, 14-21 (2007).

Alexis, F., Basto, P., Levy-Nissenbaum, E., Radovic-Moreno, A., Zhang, L., Langer, R., Molnar, L., Farokhzad, O. C.,”HER-2 Targeted Nanoparticle-Affibody Bioconjugates for Breast Cancer Therapy“, ChemMedChem. 3, 1839-1843 (2008).

Zhang, L., Chan, J. M., Gu, F.X., Rhee, J-W., Wang, A.Z., Radovic-Moreno, A., Alexis, F., Langer, R, Farokhzad, O. C., “Self-Assembled Lipid-Polymer Hybrid Nanoparticles: A Robust Drug Delivery Platform”, ACS Nano 2, 1696-1702 (2008).

Alexis, F., Radovic-Moreno, A., Rhee, J-W, Richie, J. P., Langer, R., Farokhzad, O. C., "New Frontiers in Nanotechnology for Cancer Treatment", Journal of Urological Oncology 26. 74-85 (2008).

Wang, A. Z., Bagalkot, V., Vasilliou, C., Gu, F.X., Alexis, F., Zhang, L., Shaikh M., Yuet, K. , Cima, M., Langer, R., Farokhzad, O. C., Kantoff, P.W., Bander, N.H., Jon, S., Farokhzad, O. C., "Superparamagnetic Iron Oxide Nanoparticle-Aptamer Bioconjugates for Combined Prostate Cancer Imaging and Therapy”, ChemMedChem. 9, 1311-1315 (2008).

Alexis, F., Pridgen, E., Langer, R., Farokhzad, O. C., "Factors Affecting the Clearance and Biodistribution of Polymeric Nanoparticles", Molecular Therapeutics 5, 505-515 (2008).

Conference Proceedings: (Peer Reviewed) Banik, B.L., Rogers, M., Dhulekar, J., Moore, T.L., Alexis, F., “Synthesis and Testing of Biomolecules to Target Bone”, Proceedings of the National Conference of Undergraduate Research, Ithaca College, Ithaca, NY, (2011).

Banik, B.L., Rogers, M., Dhulekar, J., Moore, T.L., Alexis, F., “Synthesis and Testing of Biomolecules to Target Bone”, Proceedings of the ACC Meetings of Minds, University of Miami, Miami, FL, (2011).

6 Keith, K., Mattison, S., Collier, M., Lewis, R., Alexis, F. “Testing Chemotherapeutic Drugs for Synergistic Toxicity Using Several Cancer Cell Lines”, Proceedings of the ACC Meetings of Minds, University of Miami, Miami, FL, (2011).

Wright-Walker, C. J., DesJardins, J. D., Alexis, F., LaBerge, M., “Tribological Evaluation of a Drug Enriched Ultra-High Molecular Weight Polyethylene for Enhanced Total Joint Replacement Bearing Surface Functionality”, Proceedings of the Society For Biomaterials Meeting, Orlando, FL, (2011).

Mattix, B., Reese, L., Pollard, S., Anderson, J., Carpenter, K., Davda, L., Breece, J., Alexis, F.”Effects of Polymeric Nanoparticle Surface Properties on Interaction with Brain Cancer Cells”, Proceedings of the Southeast Regional IDeA Meeting, New Orleans, LA, (2011).

Mattix, B., Uvarov, O., Willi, T., Reese, L., Pollard, S., Frankel, B., Alexis, F. “ Effects of Polymeric Nanoparticle Surface Properties on Interaction with Brain Tumor Environment”, Proceedings of the SC INBRE Scientific Symposium, Columbia, SC, (2012).

Mattix, B., Reese, L., Poole, T., Visconti, R., Simionescu, D., Simionescu, A., Jenkins, L., Bruce, T., Alexis, F. “Assembly of Engineered Blood Vessels Using Magnetic Forces.” Proceedings of the SC Project for Organ Biofabrication Scientific Symposium, Columbia, SC, (2012).

Poole, T., Mattix, B, Reese, L., Alexis, F. “Magnetic Spheroids For Tissue Engineering Applications.” Proceedings of the National Conference on Undergraduate Research, Weber State University, Ogden, Utah, (2012).

Prior to Clemson: Alexis, F., Rath, S. K., Boey, F.Y.C., Venkatraman, S., “Factors Affecting the Drug Release Mechanisms of PLGA 53/47 and PLLA”, Proceedings of the International Conference of Materials Processing for Properties and Performance (MP3), Singapore, Singapore, (2002).

Alexis, F., Rath, S. K., Boey, F.Y.C., Venkatraman, S., “Comparative Study of Drug Release from Matrices Made of PLLA and PLGA Copolymers”; Proceedings of the International Conference on Materials for Advanced Technologies, Singapore, Singapore, (2003).

Alexis, F., “HER-2 Targeted Nanoparticle-Affibody Bioconjugates for Breast Cancer Therapy”, Proceedings of the American Association Cancer Research Annual Meeting, Los Angeles, CA, (2007).

7 Alexis, F., “HER-2 Targeted Polymeric Nanoparticles for Breast Cancer Therapy”, Proceedings of the Nano Science and Technology Institute Conference, Santa Clara, CA, (2007).

Conference Proceedings (Unreviewed) Banik, B.L., Rogers, M., Moore, T.L., Alexis, F., “Synthesis and Testing of Biomolecules to Target Bone”, Southeast Biomedical Engineering Career Conference, Clemson, SC, (2010).

Moore, T., Przestrzelski, B., Lewis, R., Jelen, E., Dhulekar, J., Stoner, B., Bloomquist, C., Simionescu, D., Simionescu, A., Recknor, C., Alexis, F. “Targeted Nanoparticles to Deliver Drugs for Prolonged Periods of Time to Bone Tissue.”, SCMEDTECH Conference, Charleston, SC, (2011).

Stoner, B., Morrison, R., Przestrzelski, B., Dhulekar, J., Lewis, R., Jelen, E., Moore, T., Simionescu, D., Simionescu, A., Recknor, C., Alexis. F., “Targeted Nanoparticles to Deliver Drugs Through Controlled Release Kinetics to Bone Tissue”, SFB-Memphis Biomaterials Day, University of Memphis, Memphis, TN, (2011).

Reese, L., Mattix, B., Poole, T., Visconti, R., Simionescu, D., Simionescu, A., Jenkins, L., Bruce, T., Alexis, F., “ Magnetic Cell Spheroid For Tissue Engineering Applications”, SFB-Clemson University Biomaterials Day, Clemson University, Clemson, SC, (2011).

Poole, T., Mattix, B., Reese, L., Visconti, R., Simionescu, D., Simionescu, A., Jenkins, L. Bruce, T., Alexis, F., “Magnetic Spheroids For Tissue Engineering Applications”, Southeast Biomedical Engineering Career Conference, University of Virginia, Herndon, VA, (2011).

Uvarov, O., Mattix, B., Willi, T., Reese, L., Pollard, S., Frankel, B., Alexis, F.” Effects of Polymeric Nanoparticle Surface Properties on Interaction with Brain Tumor Environment”, SFB- Memphis Biomaterials Day, University of Memphis, Memphis, TN, (2012).

PRESENTATIONS Alexis, F., The Road of Academia, SFB-Clemson Biomaterials Day, Clemson University (07-22-2009).

Alexis, F., Nanotechnology and Biomaterials, Summer Science Engineering and Architecture educational program (SSEEA), Clemson University (08-07-2009).

Alexis, F., Targeted Nanoparticles for Drug Delivery, Clemson University Nanomaterial Group, Clemson University (08-12-2009).

8 Alexis, F., Targeted Nanoparticles for Drug Delivery, Clemson University Nanomaterial Group, Clemson University (08-12-2009).

Alexis, F., Targeted Polymeric Nanoparticles for Drug Delivery Applications, School of Materials Science, Nanyang Technological University (05-25-2010).

Alexis, F., Biomedical research and Technologies at Clemson University, School of Materials Science, Nanyang Technological University (05-24-2010).

Alexis, F., Targeted Nanoparticles for Drug Delivery, Institute of Environmental Toxicology, Clemson University (02-16-2010).

Alexis, F., Nanoparticle Drug Delivery Systems for Therapeutic Applications, Department of Regenerative Medicine, Medical University of South Carolina (12-13- 2010).

Alexis, F., Targeted Nanoparticles for Drug Delivery, Neuroscience Grand Round, Department of Neuroscience, Medical University of South Carolina (03-07-2010).

Alexis, F., Engineered Nanotechnologies for Biomedical Applications, School of Materials Science, Nanyang Technological University (06-30-2011).

Alexis, F., Engineered Nanotechnologies for Medical Applications, Department of Biological Sciences, Clemson University (11-14-2011).

Alexis, F., Engineered Nanotechnologies for Medical Applications, Department of Pharmacology and Toxicology, East Carolina University (11-17-2012).

Alexis, F., Assembly and Maturation of Engineered Linear Blood Vessels Using Magnetic Cellular Spheroids, SC Project for Organ Biofabrication Scientific Symposium, Columbia, SC (02-10-2012).

Alexis, F. Polymer Coated Carbon Nanotubes to Safely Deliver Drugs, 2012 American Chemical Society SERMACS Conference-Symposium "Chemistry of Bio-Nano Interfaces", Raleigh, NC-Invited (11-16-2012).

Alexis, F., Drug Delivery for Bone Diseases Therapy, NASA AMES Research Center, Moffett Field, CA- Invited (11-22-2012).

PATENTS “ Delivery Systems for Enhancing Drug Efficacy”, Frankel B., Alexis F., Provisional Patent (2012).

“ Magnetic Cellular Spheroids”, Alexis F., Simionescu D., Visconti R., Mattix B., Provisional Patent (2011).

9 “Graphene coatings for stents and implants”, Podila, R., Moore, T., Alexis, F., Rao, A. M. Provisional Patent (2012).

Trademark-"Call Me GloberTM": Under-represented Undergraduate Research Abroad. Alexis, F., Burg, K. (2012).

Print (Copyright)-“Tissue Engineering on the Dome!” Alexis, F., Burg, K. (2012). Licensed to Slicedtomato.

Prior to Clemson: "Targeted Drug Delivery Using Human IgG Fc Fragment", Alexis, F., Farokhzad, O. C., Kuo, T., Pridgen, E., Radovic-Moreno, A., Langer, R., PCT/US2007/85318 (Licensed by BIND Biosciences and SELECTA Biosciences).

"Poly (Amino Acid)-Targeted Drug Delivery", Alexis, F., Zhang, L., Chan, J., Basto, P., Levy-Nissembaum, E., Langer, R., O. Farokhzad, O. C., PCT/US08/05949 (Licensed by BIND Biosciences).

"Amphiphilic Compound Assisted Polymeric Particles for Targeted Delivery". Zhang, L., Radovic-Moreno, A., Gu, F.X., Alexis, F., Langer, R., Farokhzad, O. C., PCT/US2008/059480 (Licensed by BIND Biosciences).

"Oligonucleotide Systems for Targeted Intracellular Delivery", Levy-Nissenbaum, E., Farokhzad, O. C., Langer, R., Alexis, F., PCT/US2007/017539 (Licensed by BIND Biosciences).

"Adjuvant Incorporation in Immunonanotherapeutics", Alexis, F., Iannacone, M., Chi, J., Basto, P., Moseman, A., Von Andrian, U., Langer, R., Farokhzad, O.C.,PCT/US2008/11932 (Licensed by SELECTA Biosciences).

"Nicotine Immunotherapeutics", Von Andrian, U., Farokhzad, O.C., Alexis, F., Iannacone, M., Basto, P., Chi, J., Moseman, A., Langer, R., PCT/US2008/428408 (Licensed by SELECTA Biosciences).

"Engineered Antibody and Antibody Fragments for Oriented Conjugation-Method of Preparation and Uses Thereof", Alexis, F., Aizu, W., Kuo, T., Pridgen, E., Farokhzad, O.C., Langer, R., (2008), provisional patent.

"Nanoparticle Technology for the Delivery of Multiple Molecules- Methods of Preparation and Uses Thereof", Pridgen, E., Alexis, F., Westernof, K., Marein, S., Bander, N., Kantoff, P., Farokhzad, O.C., Langer, R., (2008) provisional patent.

"Vaccine Nanotechnology", Von Andrian, U., Farokhzad, O.C., Langer, R., Tobias, J., Moseman, A., Zhang, L., Basto, P., Iannacone, M., Alexis, F., (2008) (Licensed by SELECTA Biosciences).

10 "Targeting of Antigen Presenting Cells with Immunonanotherapeutics", Chi, J., Alexis, F., Iannacone, M., Moseman, A., Basto, P., Langer, R., Farokhzad, O.C., Von Andrian, U., PCT/US08/11932. (Licensed by SELECTA Biosciences).

"Immunonanotherapeutics that Provides IgG Humoral Response Without T-Cell Antigen", Iannacone, M., Alexis, F., Basto, P., Moseman, A., Chi, J Langer, R., Farokhzad, O.C., Von Andrian, U., (Licensed by SELECTA Biosciences).

HONORS AND AWARDS Third place at the Singapore Young Person’s Lecture Competition, Institute of Materials South East Asia (2004). Phage Display of Proteins and Peptides Training, Cold Spring Harbor Laboratory (2009) Most cited article in ChemMedChem (2007) Most accessed article in Molecular Pharmaceutics (2008-2012)

UNIVERSITY AND PUBLIC SERVICE Committees Department: Member, ABET (2009-2011) Member: Study Abroad Programs (2010- ) Chair: Page Morton Hunter Seminar Series (2010-) Chair: C. Dayton Riddle Seminar Series (2012-) Member: Student Award (2012-) University: Member: Eportfolio assessment (2012-) Member: Quality Enhancement Plan (2012-)

Other Service ETS Proficiency Profile /Measure of Academic Proficiency & Progress (MAPP) (2010) Phonathon, Bioengineering, (2010) ETS Proficiency Profile /Measure of Academic Proficiency & Progress (MAPP) (2011) Arizona State University-Nanotechnology and Society Survey (2011)