Nora Wirtza$, Christian Printzenb and H. Thorsten Lumbscha*

Organisms Diversity & Evolution

Using haplotype networks, estimation of gene flow and phenotypic characters to understand species delimitation in fungi of a predominantly Antarctic Usnea group (Ascomycota, Parmeliaceae)

Nora Wirtza$, Christian Printzenb and H. Thorsten Lumbscha* aDepartment of Botany, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605- 2496, U.S.A. bAbteilung Botanik und Molekulare Evolutionsforschung, Forschungsinstitut Senckenberg, Senckenberganlage 25, 60325 Frankfurt/Main, Germany $Current address: 11 Grosvenor Mount, Leeds LS6 2DX, United Kingdom

*Corresponding author. Telephone: +1 312 665 7881; Fax: +1 312 665 7258. E-mail address: [email protected] (H.T. Lumbsch)

Supplementary Material

Table 1: Detailed taxon sampling of the Usnea aurantiaco-atra group, U. sphacelata group and outgroups. Genbank Accession numbers of all newly generated ITS, IGS and RPB1 sequences are in bold. Taxa not included in the network analyses are labeled by asterisk. TdF= Tierra del Fuego, AR= Argentina, AN= Antarctica, NZ= New Zealand. 2

Sample # Sampling Site Collector/Date Genbank Accession #

ITS IGS RPB1 Usnea aurantiaco-atra group Usnea acromelana 237-2 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314821 JQ315013 JQ314588 175-2 TdF, Glaciar Martial H.T. Lumbsch 2002 DQ235515 JF283520 JF283486 175-3* TdF, Glaciar Martial H.T. Lumbsch 2002 JQ314848 JQ315040 JQ314673 175-4* TdF, Glaciar Martial H.T. Lumbsch 2002 JQ314849 JQ315041 JQ314674 175-5 TdF, Glaciar Martial H.T. Lumbsch 2002 JQ314828 JQ315020 JQ314634 184-2 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314767 JQ314952 JQ314497 184-3 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 DQ235514 JF283519 JF283485 184-5 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314768 JQ314953 JQ314498 253-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314826 JQ315018 JQ314654 253-2 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314827 JQ315019 JQ314655

188-1 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 DQ235516 JF283518 JF283484 188-12 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314765 JQ314950 JQ314495 188-15 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314766 JQ314951 JQ314496 Usnea aurantiaco-atra/ Usnea antarctica 72 AN, Terra Firma S. Ott 01/2001 DQ235523 JF283513 JF283479

108-1 AN, Leonie Island Mairi 02/2001 JQ314813 JQ315004 JQ314549 111-1 AN, Leonie Island Mairi 02/2001 JQ314814 JQ315005 JQ314550

96-4 AN, Adelaide Island, Rothera R.I.L. Smith 1998 JQ314810 JQ315001 JQ314546 97-1 AN, Adelaide Island, Rothera R.I.L. Smith 1998 JQ314811 JQ315002 JQ314547 97-6 AN, Adelaide Island, Rothera R.I.L. Smith 1998 JQ314812 JQ315003 JQ314548

144-1 AN, Deception Island B. Schroeter 02/2002 JQ314791 JQ314979 JQ314525 144-5 AN, Deception Island B. Schroeter 02/2002 JQ314792 JQ314980 JQ314526 147-1 AN, Deception Island B. Schroeter 02/2002 JF283508 JF283516 JF283482 147-3 AN, Deception Island B. Schroeter 02/2002 JQ314837 JQ315031 JQ314564 147-8 AN, Deception Island B. Schroeter 02/2002 JQ314838 JQ315032 JQ314565 147-12 AN, Deception Island B. Schroeter 02/2002 JQ314804 JQ314993 JQ314540 95-1 AN, Deception Island R.I.L. Smith 2002 JQ314807 JQ314998 JQ314544 95-3 AN, Deception Island R.I.L. Smith 2002 JQ314808 JQ314999 JQ314545

121-3 AN, Livingston Island H.T. Lumbsch 2002 JQ314819 JQ315011 JQ314555 121-4 AN, Livingston Island H.T. Lumbsch 2002 JQ314820 JQ315012 JQ314556 123-1 AN, Livingston Island H.T. Lumbsch 2002 JF283507 JF283515 JF283481 125-8 AN, Livingston Island H.T. Lumbsch 2002 JQ314832 JQ315026 JQ314559 128-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314833 JQ315027 JQ314560 137-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314775 JQ314960 JQ314508 137-2 AN, Livingston Island H.T. Lumbsch 2002 EF179795 JQ314961 EF179782 137-3 AN, Livingston Island H.T. Lumbsch 2002 JQ314776 JQ314962 JQ314509 139-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314782 JQ314968 JQ314515 139-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314783 JQ314969 JQ314516 139-5 AN, Livingston Island H.T. Lumbsch 2002 EF116567 JQ314970 EF116565 139-6 AN, Livingston Island H.T. Lumbsch 2002 JQ314784 JQ314971 JQ314517 120-3 AN, Livingston Island H.T. Lumbsch 2002 JQ314817 JQ315008 JQ314553 120-4 AN, Livingston Island H.T. Lumbsch 2002 JQ314818 JQ315009 JQ314554 120-5 AN, Livingston Island H.T. Lumbsch 2002 EF179789 JQ315010 EF179785 133-6 AN, Livingston Island H.T. Lumbsch 2002 JQ314834 JQ315028 JQ314561 124-1 AN, Livingston Island H.T. Lumbsch 2002 EF179799 JQ315023 EF179786 124-4 AN, Livingston Island H.T. Lumbsch 2002 DQ235521 JQ315024 JQ314557 124-5 AN, Livingston Island H.T. Lumbsch 2002 JQ314831 JQ315025 JQ314558 135-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314835 JQ315029 JQ314562 135-4 AN, Livingston Island H.T. Lumbsch 2002 JQ314836 JQ315030 JQ314563 3

ITS IGS RPB1 136-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314772 JQ314957 JQ314505 136-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314773 JQ314958 JQ314506 136-3 AN, Livingston Island H.T. Lumbsch 2002 JQ314774 JQ314959 JQ314507 138-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314777 JQ314963 JQ314510 138-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314778 JQ314964 JQ314511 138-3 AN, Livingston Island H.T. Lumbsch 2002 JQ314779 JQ314965 JQ314512 138-4 AN, Livingston Island H.T. Lumbsch 2002 JQ314780 JQ314966 JQ314513 138-5 AN, Livingston Island H.T. Lumbsch 2002 JQ314781 JQ314967 JQ314514 138-6 AN, Livingston Island H.T. Lumbsch 2002 DQ235517 JF283512 JF283478 140-1 AN, Livingston Island H.T. Lumbsch 2002 JQ314785 JQ314972 JQ314518 140-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314786 JQ314973 JQ314519 142-2 AN, Livingston Island H.T. Lumbsch 2002 JQ314787 JQ314974 JQ314520 142-3 AN, Livingston Island H.T. Lumbsch 2002 DQ235518 JQ314975 JQ314521 142-4 AN, Livingston Island H.T. Lumbsch 2002 JQ314788 JQ314976 JQ314522 142-5 AN, Livingston Island H.T. Lumbsch 2002 JQ314789 JQ314977 JQ314523 142-6 AN, Livingston Island H.T. Lumbsch 2002 JQ314790 JQ314978 JQ314524

112-1 AN, King George Island K. Láska 01/2000 JQ314815 JQ315006 JQ314551 112-2 AN, King George Island K. Láska 01/2000 JQ314816 JQ315007 JQ314552 113-2 AN, King George Island J. Gloser 01/2001 JQ314771 JQ314956 JQ314504

92-1 South Orkney Islands, Signy R.I.L. Smith 2000 JQ314806 JQ314997 JQ314543 Island

107 Falkland Islands, Port P. Crittenden 2001 EF179797 JQ314996 EF179784 Stanley

148-1 TdF, Lapataia Bay H.T. Lumbsch 2002 EF179796 JQ314981 EF179783 148-2 TdF, Lapataia Bay H.T. Lumbsch 2002 JQ314793 JQ314982 JQ314527 148-3 TdF, Lapataia Bay H.T. Lumbsch 2002 JQ314794 JQ314983 JQ314528 234-3 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314809 JQ315000 JQ314671 235-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314842 JQ315036 JQ314569 235-2 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314843 JQ315037 JQ314570 236-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 DQ235524 JF283514 JF283480 238-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314822 JQ315014 JQ314589 239-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314823 JQ315015 JQ314590 240-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314844 JQ315038 JQ314571 240-2 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 DQ235522 JQ315039 JQ314572 241-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314825 JQ315017 JQ314591 181-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314839 JQ315033 JQ314566 181-4 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314840 JQ315034 JQ314567 183-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314795 JQ314984 JQ314529 183-2 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314796 JQ314985 JQ314530 256-11 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314805 JQ314995 JQ314542 256-14 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 DQ235519 JF283517 JF283483 199-11 TdF, Paso Garibaldi N. Wirtz, M.I. Messuti 12/2003 DQ235520 JQ314994 JQ314541 199-5 TdF, Paso Garibaldi N. Wirtz, M.I. Messuti 12/2003 JQ314841 JQ315035 JQ314568

194-1 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314797 JQ314986 JQ314531 Aymond 194-2 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314798 JQ314987 JQ314532 Aymond 194-3 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314799 JQ314988 JQ314533 Aymond 194-4 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314800 JQ314989 JQ314534 Aymond 194-5 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314801 JQ314990 JQ314535 Aymond 4

ITS IGS RPB1 194-6 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314802 JQ314991 JQ314536 Aymond 194-7 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314803 JQ314992 JQ314537 Aymond 194-10 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314770 JQ314955 JQ314503 Aymond

189-1 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314824 JQ315016 JQ314577 200-4 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314769 JQ314954 JQ314499 206-1 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314829 JQ315021 JQ314579 207-1 AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314830 JQ315022 JQ314580

Usnea sphacelata group Usnea sphacelata 189-3* AR, Santa Cruz, El Chalten N. Wirtz, M.I. Messuti 12/2003 JQ314846 JQ315042 JQ314675

220-3 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314704 JQ314852 JQ314581 Catedral 220-4 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314705 JQ314853 JQ314582 Catedral 225-1 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 DQ235495 JF283523 JF283489 Catedral 225-2 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314707 JQ314855 JQ314583 Catedral 226-1 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314706 JQ314854 JQ314584 Catedral 226-6 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314708 JQ314856 JQ314586 Catedral

50 Ecuador, Chimborazo Kulísek, Stancík 2000 JQ314713 JQ314861 JQ314603 52 Ecuador, Chimborazo Z. Palice 1999 JQ314701 JQ314862 JQ314604 53 Ecuador, Chimborazo Z. Palice 1999 JQ314714 JQ314863 JQ314605 54 Ecuador, Chimborazo Z. Palice 1999 JQ314700 JQ314864 JQ314606 56 Ecuador, Chimborazo Z. Palice, Soldán 2000 JQ314715 JQ314865 JQ314607

244-1 Canada, Melville Island G.W. Scotter 07/1992 JQ314742 JQ314898 JQ314646 244-2 Canada, Melville Island G.W. Scotter 07/1992 JQ314743 JQ314899 JQ314647 244-3 Canada, Melville Island G.W. Scotter 07/1992 JQ314744 JQ314900 JQ314648 244-5 Canada, Melville Island G.W. Scotter 07/1992 JQ314745 JQ314901 JQ314649

149-1 Iceland, Central Highlands S. Heiđmarsson 08/2002 JQ314722 JQ314876 JQ314616 150-1 Iceland, Central Highlands H. Arinbjarnarson 9/2002 JQ314723 JQ314877 JQ314617 150-2 Iceland, Central Highlands H. Arinbjarnarson 9/2002 JQ314724 JQ314878 JQ314618 153-2 Iceland, Central Highlands S. Heiđmarsson 2002 JQ314729 JQ314884 JQ314624 152-4 Iceland, Central Highlands S. Heiđmarsson 2002 DQ235493 JQ314881 JQ314621 152-5 Iceland, Central Highlands S. Heiđmarsson 2002 JQ314727 JQ314882 JQ314622 152-6 Iceland, Central Highlands S. Heiđmarsson 2002 JQ314728 JQ314883 JQ314623 151-1 Iceland, Central Highlands S. Heiđmarsson 2002 JQ314725 JQ314879 JQ314619 151-2 Iceland, Central Highlands S. Heiđmarsson 2002 JQ314726 JQ314880 JQ314620

243-1 Greenland, Disko A.V. Larsen 07/1996 JQ314740 JQ314896 JQ314644 243-2 Greenland, Disko A.V. Larsen 07/1996 DQ235494 JF283521 JF283487 243-3 Greenland, Disko A.V. Larsen 07/1996 JQ314741 JQ314897 JQ314645

245-1 Greenland, Thule V. Alstrup 08/1992 JQ314746 JQ314902 JQ314650 245-2 Greenland, Thule V. Alstrup 08/1992 JQ314747 JQ314903 JQ314651 5

ITS IGS RPB1 245-3 Greenland, Thule V. Alstrup 08/1992 JQ314748 JQ314904 JQ314652 245-4 Greenland, Thule V. Alstrup 08/1992 JQ314749 JQ314905 JQ314653

114-2 Spitsbergen, Svalbard T. Tønsberg 07/2002 JQ314717 JQ314869 JQ314611 115-5 Spitsbergen, Svalbard T. Tønsberg 07/2002 JQ314718 JQ314870 JQ314612 115-8 Spitsbergen, Svalbard T. Tønsberg 07/2002 EF179802 JQ314871 EF179789 115-11 Spitsbergen, Svalbard T. Tønsberg 07/2002 JQ314709 JQ314857 JQ314599 116-1 Spitsbergen, Svalbard T. Tønsberg 07/2002 JQ314719 JQ314872 JQ314613 116-2 Spitsbergen, Svalbard T. Tønsberg 07/2002 JQ314720 JQ314873 JQ314614 117-1 Spitsbergen, Svalbard C. Printzen 2002 DQ235492 JF283522 JF283488 117-2 Spitsbergen, Svalbard C. Printzen 2002 JQ314721 JQ314874 JQ314615 118-1 Spitsbergen, Svalbard C. Printzen 2002 EF179803 JQ314875 EF179790 119-1* Spitsbergen, Svalbard C. Printzen 2002 JQ314847 JQ315043 JQ314676 Usnea subantarctica 73 AN, Alexander Island, Mars S.Ott 01/2001 EF179806 JQ314867 EF179794 Oasis 78* AN, Alexander Island, Mars S.Ott 01/2001 DQ235506 JQ315044 JQ314677 Oasis 233-1 AN, Alexander Island, Mars F. Seymour JQ314737 JQ314893 JQ314641 Oasis 291-1 AN, Alexander Island, Mars S.Ott 01/2001 JQ314750 JQ314906 JQ314656 Oasis

165-2 AN, Lagoon Island S. Ott 1999 DQ235508 JF283526 JF283492

234-2 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314763 JQ314922 JQ314670 264-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314764 JQ314923 JQ314672 265-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JF283509 JF283525 JF283491 182-2 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314754 JQ314911 JQ314660

190-2 AR, Santa Cruz, El Chalten N. Wirtz/ M.I. Messuti 12/2003 JQ314759 JQ314917 JQ314666 200-1 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314732 JQ314887 JQ314635 203-1 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314733 JQ314888 JQ314636 203-11 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314710 JQ314858 JQ314600 203-13 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314711 JQ314859 JQ314601 203-14 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314712 JQ314860 JQ314602 203-2 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314734 JQ314889 JQ314637 203-3 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 DQ235507 JQ314890 JQ314638 203-6 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314735 JQ314891 JQ314639 203-9 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 JQ314736 JQ314892 JQ314640 205-1 AR, Santa Cruz, El Chalten N. Wirtz/ M.I.Messuti 12/2003 DQ235505 JF283524 JF283490 Usnea trachycarpa 102-1 AN, Alexander Island, Mars R.I.L. Smith 1997 JQ314716 JQ314868 JQ314610 Oasis 233-2 AN, Alexander Island, Mars F. Seymour JQ314738 JQ314894 JQ314642 Oasis 233-3 AN, Alexander Island, Mars F. Seymour JQ314739 JQ314895 JQ314643 Oasis 174-5 AN, Alexander Island, Mars S. Ott 12/1998 JQ314731 JQ314886 JQ314633 Oasis

162-2 AN, Leonie Island S. Ott 2001 JQ314730 JQ314885 JQ314625

164-1 AN, Lagoon Island S. Ott 2001 EF179804 JQ314907 EF179791 6

ITS IGS RPB1 234-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 EF116568 JQ314921 EF116566 237-1 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 JQ314703 JQ314851 JQ314587 173-1 TdF, Lapataia Bay H.T. Lumbsch 2002 DQ235496 JF283529 EF179793 173-2 TdF, Lapataia Bay H.T. Lumbsch 2002 JQ314751 JQ314908 JQ314657 173-3 TdF, Lapataia Bay H.T. Lumbsch 2002 JQ314752 JQ314909 JQ314658 182-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 JQ314753 JQ314910 JQ314659 182-3 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 DQ235497 JF283527 JF283493

186-2 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314755 JQ314912 JQ314661 Aymond 186-3 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 DQ235509 JQ314913 JQ314662 Aymond 186-8 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314756 JQ314914 JQ314663 Aymond 186-9 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314757 JQ314915 JQ314664 Aymond 196-2 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314760 JQ314918 JQ314667 Aymond 196-3 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314761 JQ314919 JQ314668 Aymond 197-1 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314762 JQ314920 JQ314669 Aymond 197-3 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 DQ235498 JF283528 JF283494 Aymond

190-1 AR, Santa Cruz, El Chalten N. Wirtz, M.I.Messuti 12/2003 JQ314758 JQ314916 JQ314665

226-4 AR, Rio Negro, Bariloche, C. N. Wirtz, M.I. Messuti 12/2003 JQ314702 JQ314850 JQ314585 Catedral

57 Ecuador, Chimborazo Z. Palice, Soldán 2000 JQ314699 JQ314866 JQ314608

Outgroups Usnea acanthella 268-1 Peru, Cusco, Challabamba A. Ramírez 09/04 DQ235480 JF283535 JF283500 269-1 Peru, Cusco, Challabamba A. Ramírez 09/04 JQ314697 JQ314928 JQ314538

289-1 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235482 JF283534 JF283499 Huascaran, Quebrada Ulta Ramírez 11/2004 289-2 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314698 JQ314929 JQ314539 Huascaran, Quebrada Ulta Ramírez 11/2004

168-1 Ecuador, Tungurahua, Z. Palice 03/2003 JQ314689 JQ314943 JQ314626 Llangamates

169-1 Ecuador, Carchi, Volcano Z. Palice 03/2003 JQ314690 JQ314944 JQ314627 Chiles 169-2 Ecuador, Carchi, Volcano Z. Palice 03/2003 DQ235483 JF283533 JF283498 Chiles 169-3 Ecuador, Carchi, Volcano Z. Palice 03/2003 JQ314691 JQ314945 JQ314628 Chiles 171-2 Ecuador, Carchi, Volcano Z. Palice 03/2003 JQ314692 JQ314946 JQ314629 Chiles 171-3 Ecuador, Carchi, Volcano Z. Palice 03/2003 JQ314693 JQ314947 JQ314630 Chiles 171-4 Ecuador, Carchi, Volcano Z. Palice 03/2003 DQ235481 JQ314948 JQ314631 Chiles 7

ITS IGS RPB1 171-5 Ecuador, Carchi, Volcano Z. Palice 03/2003 JQ314694 JQ314949 JQ314632 Chiles Usnea patagonica 191-1 AR, Santa Cruz, El Calafate N. Wirtz, M.I. Messuti 12/2003 JQ314680 JQ314931 JQ314574 191-2 AR, Santa Cruz, El Calafate N. Wirtz, M.I. Messuti 12/2003 JQ314681 JQ314932 JQ314575 191-3 AR, Santa Cruz, El Calafate N. Wirtz, M.I. Messuti 12/2003 DQ235485 JF283532 JF283497 191-4 AR, Santa Cruz, El Calafate N. Wirtz, M.I. Messuti 12/2003 JQ314682 JQ314933 JQ314576

195-1 AR, Santa Cruz, Monte N. Wirtz, M.I. Messuti 12/2003 JQ314683 JQ314934 JQ314578 Aymond

266-1 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314684 JQ314935 JQ314592 Huascaran, Huarapasca Ramírez 11/2004 266-2 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235486 JF283531 JF283496 Huascaran, Huarapasca Ramírez 11/2004 266-3 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314685 JQ314936 JQ314593 Huascaran, Huarapasca Ramírez 11/2004 290-11 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235484 JQ314930 JQ314573 Huascaran, Huarapasca Ramírez 11/2004

62 Ecuador, Chimborazo Z. Palice 1999 EF179801 JQ314941 EF179788 63 Ecuador, Chimborazo Z. Palice 1999 DQ235487 JF283530 JF283495 67 Ecuador, Chimborazo Z. Palice 1999 JQ314688 JQ314942 JQ314609 Usnea sp. 267-1 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314696 JQ314925 JQ314500 Huascaran, Quebrada Ulta Ramírez 11/2004 267-2 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235499 JQ314926 JQ314501 Huascaran, Quebrada Ulta Ramírez 11/2004 267-3 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235500 JQ314927 JQ314502 Huascaran, Quebrada Ulta Ramírez 11/2004 288-1 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235502 JQ314937 JQ314595 Huascaran, Quebrada Ulta Ramírez 11/2004 288-2 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314686 JQ314938 JQ314596 Huascaran, Quebrada Ulta Ramírez 11/2004 288-3 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. DQ235501 JQ314939 JQ314597 Huascaran, Quebrada Ulta Ramírez 11/2004 288-4 Peru, Ancash, P. N. N. Wirtz, H.T. Lumbsch & A. JQ314687 JQ314940 JQ314598 Huascaran, Quebrada Ulta Ramírez 11/2004 Usnea from New Zealand 276-1 NZ, Otago, Remarkables A. Marky 04/1999 DQ235475 JF283539 JF283504

281-3 NZ, Otago, Pisa Range A. Knight 04/2003 JQ314845 JQ315045 JQ314678 282-1 NZ, Otago, Pisa Range A. Knight 04/2003 JQ314695 JQ314924 JQ314594

283-1 NZ, Otago, Old Man Range D.J. Galloway 02/1999 DQ235477 JF283536 JF283501 285-2 NZ, Otago, Old Man Range K. Spencer 11/2004 DQ235479 JQ315046 JQ314679 286-1 NZ, Otago, Old Man Range K. Spencer 11/2004 DQ235478 JF283537 JF283502 287-1 NZ, Otago, Old Man Range K. Spencer 11/2004 DQ235476 JF283540 JF283505 8

Table 2: Taxon sampling of the Usnea perpusilla complex (see also Table 1 in Wirtz et al. 2008) and Genbank accession numbers of the previously released ITS, IGS and RPB1 sequences. TdF= Tierra del Fuego, AR= Argentina, AN= Antarctica. 9

ITS IGS RPB1

Usnea perpusilla AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492147 EF528324 EF528404 192-21 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492155 EF528335 EF528415 208-1 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492156 EF528336 EF528416 208-2 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235490 EF528327 EF528407 208-19 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492215 EF528400 EF528479 218-1 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235488 EF528401 EF528480 218-2 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492216 EF528402 EF528481 218-3 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235489 EF528342 EF528422 221-1 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492161 EF528343 EF528423 221-2 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492150 EF528329 EF528409 221-3b Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492151 EF528330 EF528410 221-3c Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492153 EF528332 EF528412 222-10 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492162 EF528344 EF528424 222-2 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492165 EF528347 EF528427 227-1 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235491 EF528348 EF528428 227-4 Bariloche, C. Catedral AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492166 EF528349 EF528429 227-7 Bariloche, C. Catedral Usnea ushuaiensis 235-11 TdF, Lapataia Bay N. Wirtz, M.I. Messuti 12/2003 EF492146 EF528323 EF528403 179-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492210 EF528395 EF528474 179-3 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492211 EF528396 EF528475 179-4 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492212 EF528397 EF528476 179-7 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492213 EF528398 EF528477 180-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492170 EF528353 EF528433 258-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492167 EF528350 EF528430 258-2 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492168 EF528351 EF528431 263-1 TdF, Glaciar Martial N. Wirtz, M.I. Messuti 12/2003 EF492182 EF528366 EF528446 Usnea messutiae 189-2 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492171 EF528354 EF528434 Chalten 190-3 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492214 EF528399 EF528478 Chalten 201-1 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492172 EF528355 EF528435 Chalten 201-2 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492173 EF528356 EF528436 Chalten 204-1 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492174 EF528357 EF528437 Chalten 10

ITS IGS RPB1 204-2 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492175 EF528358 EF528438 Chalten 204-4 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492176 EF528359 EF528439 Chalten 205-3 AR, Santa Cruz, El N. Wirtz, M.I. Messuti 12/2003 EF492177 EF528360 EF528440 Chalten

209-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492208 EF528393 EF528472 Bariloche, C. Catedral 209-3 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492209 EF528394 EF528473 Bariloche, C. Catedral

41 Ecuador, Chimborazo Z. Palice, Soldán 2000 EF492183 EF528367 EF528447 46 Ecuador, Chimborazo Z. Palice, Soldán 2000 EF492184 EF528369 EF528448 47 Ecuador, Chimborazo Z. Palice, Soldán 2000 EF492185 EF528370 EF528449 66 Ecuador, Chimborazo Z. Palice 1999 EF492187 EF528372 EF528451 Usnea lambii 166-2 AN, Victoria Land, L. Sancho, A. Green 01/2003 EF492189 EF528374 EF528453 Nunatak 166-3 AN, Victoria Land, L. Sancho, A. Green 01/2003 EF492190 EF528375 EF528454 Nunatak 167-1 AN, Victoria Land, L. Sancho, A. Green 01/2003 EF492191 EF528376 EF528455 Nunatak 167-2 AN, Victoria Land, L. Sancho, A. Green 01/2003 EF492192 EF528377 EF528456 Nunatak 167-6 AN, Victoria Land, L. Sancho, A. Green 01/2003 EF492193 EF528378 EF528457 Nunatak

178-1 AN, Casey Station P. Crittenden 1995 EF492194 EF528379 EF528458 178-2 AN, Casey Station P. Crittenden 1995 EF492195 EF528380 EF528459

103-2 AN, Alexander Island, R.I.L. Smith 1997 EF492188 EF528373 EF528452 Mars Oasis 232-1 AN, Alexander Island, F. Seymour EF492200 EF528385 EF528464 Mars Oasis 232-2 AN, Alexander Island, F. Seymour EF492201 EF528386 EF528465 Mars Oasis 232-3 AN, Alexander Island, F. Seymour EF492202 EF528387 EF528466 Mars Oasis 292-1 AN, Alexander Island, S.Ott 01/2001 EF492169 EF528352 EF528432 Mars Oasis

229-1 AN, Adelaide Island, F. Seymour EF492196 EF528381 EF528460 Reptile Ridge 230-1 AN, Adelaide Island, F. Seymour EF492197 EF528382 EF528461 Rothera 230-2 AN, Adelaide Island, F. Seymour EF492198 EF528383 EF528462 Rothera 230-3 AN, Adelaide Island, F. Seymour EF492199 EF528384 EF528463 Rothera

211-2 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492178 EF528361 EF528441 Bariloche, C. Catedral 212-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492179 EF528362 EF528442 Bariloche, C. Catedral 215-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492158 EF528339 EF528419 Bariloche, C. Catedral 11

ITS IGS RPB1 215-2 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492159 EF528340 EF528420 Bariloche, C. Catedral 216-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492160 EF528341 EF528421 Bariloche, C. Catedral 219-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492180 EF528364 EF528444 Bariloche, C. Catedral 219-2 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492181 EF528365 EF528445 Bariloche, C. Catedral 221-3a AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492149 EF528328 EF528408 Bariloche, C. Catedral 221-3d AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492152 EF528331 EF528411 Bariloche, C. Catedral 223-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492163 EF528345 EF528425 Bariloche, C. Catedral 224-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492164 EF528346 EF528426 Bariloche, C. Catedral

42 Ecuador, Chimborazo Z. Palice, Soldán 2000 EF179800 EF528368 EF179787 60 Ecuador, Chimborazo Z. Palice, Soldán 2000 EF492186 EF528371 EF528450

250-2 USA, Oregon, Mt. B. McCune 07/1994 EF492203 EF528388 EF528467 Hood 250-3 USA, Oregon, Mt. B. McCune 07/1994 EF492204 EF528389 EF528468 Hood

251-2 USA, Washington, Mt. B. McCune 08/1999 EF492205 EF528390 EF528469 Adams 251-3 USA, Washington, Mt. B. McCune 08/1999 EF492206 EF528391 EF528470 Adams 251-4 USA, Washington, Mt. B. McCune 08/1999 EF492207 EF528392 EF528471 Adams Usnea pallidocarpa 193-2 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235512 EF528333 EF528413 Bariloche, C. Catedral 193-5 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492154 EF528334 EF528414 Bariloche, C. Catedral 193-12 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235510 EF528325 EF528405 Bariloche, C. Catedral 193-13 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492148 EF528326 EF528406 Bariloche, C. Catedral 210-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235511 EF528337 EF528417 Bariloche, C. Catedral 210-2 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 EF492157 EF528338 EF528418 Bariloche, C. Catedral 213-1 AR, Rio Negro, N. Wirtz, M.I. Messuti 12/2003 DQ235513 EF528363 EF528443 Bariloche, C. Catedral