Primer 6 Software Package

Primer 6 software package

November – April Season

MDS Non-metric Multi-Dimensional Scaling

Resemblance worksheet Name: Resem4 Data type: Similarity Selection: All

Parameters Kruskal stress formula: 1 Minimum stress: 0.01

Best 3-d configuration (Stress: 0) Sample 1 2 3 % lower_M 1.03 -0.69 0.39 0.0 lower_W 0.69 0.06 -0.63 0.0 middl_M 0.45 0.50 0.05 0.0 middl_W -0.23 0.79 0.29 0.0 upper_M -0.89 -0.41 -0.22 0.0 upper_W -1.06 -0.25 0.12 0.0

Best 2-d configuration (Stress: 0) Sample 1 2 % lower_M -1.50 -0.12 0.0 lower_W -0.51 -0.51 0.0 middl_M -0.34 0.36 50.0 middl_W 0.16 0.66 0.0 upper_M 1.04 -0.35 25.0 upper_W 1.15 -0.05 25.0

STRESS VALUES Repeat 3D 2D 1 0.01 0.01 2 0 0.01 3 0.01 0.01 4 0 0.13 5 0 0.13 6 0.01 0.01 7 0.01 0.01 8 0.01 0.01 9 0.01 0.01 10 0.01 0.13 11 0.01 0.01 12 0.01 0 13 0 0 14 0.01 0.01 15 0.15 0.01 16 0.01 0.01 17 0.01 0.01 18 0.01 0.01 19 0.01 0 20 0.01 0.01 21 0 0.01 22 0.01 0.01 23 0 0.01 24 0.01 0 25 0.01 0.01

** = Maximum number of iterations used

3-d : Minimum stress: 0 occurred 6 times 2-d : Minimum stress: 0 occurred 4 times

Outputs Plot: Graph7 Plot: Graph8

ANOSIM Analysis of Similarities

Two-Way Crossed Analysis

Factor Values Factor: section lower middl upper Factor: stratum W M

Factor Groups Sample section stratum 11_lower_W lower W 12_lower_W lower W 1_lower_W lower W 2_lower_W lower W 3_lower_W lower W 4_lower_W lower W 11_lower_M lower M 12_lower_M lower M 1_lower_M lower M 2_lower_M lower M 3_lower_M lower M 4_lower_M lower M 11_middl_W middl W 12_middl_W middl W 1_middl_W middl W 2_middl_W middl W 3_middl_W middl W 4_middl_W middl W 11_middl_M middl M 12_middl_M middl M 1_middl_M middl M 2_middl_M middl M 3_middl_M middl M 4_middl_M middl M 11_upper_W upper W 12_upper_W upper W 1_upper_W upper W 2_upper_W upper W 3_upper_W upper W 4_upper_W upper W 11_upper_M upper M 12_upper_M upper M 1_upper_M upper M 2_upper_M upper M 3_upper_M upper M 4_upper_M upper M

TESTS FOR DIFFERENCES BETWEEN section GROUPS (across all stratum groups) Global Test Sample statistic (Global R): 0.438 Significance level of sample statistic: 0.1% Number of permutations: 999 (Random sample from a large number) Number of permuted statistics greater than or equal to Global R: 0

Pairwise Tests R Significance Possible Actual Number >= Groups Statistic Level % Permutations Permutations Observed lower, middl 0.411 0.1 213444 999 0 lower, upper 0.557 0.1 213444 999 0 middl, upper 0.369 0.1 213444 999 0

TESTS FOR DIFFERENCES BETWEEN stratum GROUPS (across all section groups) Global Test Sample statistic (Global R): 0.336 Significance level of sample statistic: 0.1% Number of permutations: 999 (Random sample from 98611128) Number of permuted statistics greater than or equal to Global R: 0

Outputs Plot: Graph15 Plot: Graph16 May – October Season

MDS Non-metric Multi-Dimensional Scaling

Parameters Kruskal stress formula: 1 Minimum stress: 0.01

Best 3-d configuration (Stress: 0) Sample 1 2 3 % lower_M -1.31 0.36 0.24 0.0 lower_W -0.72 0.02 -0.58 0.0 middl_M -0.37 -0.37 0.25 0.0 middl_W 0.35 -0.63 0.07 0.0 upper_M 0.88 0.46 0.18 0.0 upper_W 1.15 0.15 -0.16 0.0

Best 2-d configuration (Stress: 0) Sample 1 2 % lower_M 1.15 0.62 0.0 lower_W 0.93 -0.11 0.0 middl_M 0.27 -0.45 50.0 middl_W -0.33 -0.82 0.0 upper_M -0.95 0.49 25.0 upper_W -1.07 0.27 25.0

STRESS VALUES Repeat 3D 2D 1 0.01 0.01 2 0.01 0.01 3 0.01 0.01 4 0.01 0.01 5 0.01 0.01 6 0.01 0.02 7 0 0.01 8 0.01 0.01 9 0.15 0.27 10 0 0.01 11 0.01 0.01 12 0.01 0.01 13 0.01 0.02 14 0.01 0.13 15 0 0.01 16 0.01 0.01 17 0.01 0 18 0 0 19 0.01 0 20 0.01 0.01 21 0.01 0.13 22 0.01 0.01 23 0 0.01 24 0.01 0.01 25 0.01 0.01

** = Maximum number of iterations used

3-d : Minimum stress: 0 occurred 5 times 2-d : Minimum stress: 0 occurred 3 times

ANOSIM Analysis of Similarities

Two-Way Crossed Analysis

Factor Values Factor: section lower middl upper Factor: stratum M W

Factor Groups Sample section stratum 5_lower_M lower M 6_lower_M lower M 7_lower_M lower M 8_lower_M lower M 9_lower_M lower M 10_lower_M lower M 5_lower_W lower W 6_lower_W lower W 7_lower_W lower W 8_lower_W lower W 9_lower_W lower W 10_lower_W lower W 5_middl_M middl M 6_middl_M middl M 7_middl_M middl M 8_middl_M middl M 9_middl_M middl M 10_middl_M middl M 5_middl_W middl W 6_middl_W middl W 7_middl_W middl W 8_middl_W middl W 9_middl_W middl W 10_middl_W middl W 5_upper_M upper M 6_upper_M upper M 7_upper_M upper M 8_upper_M upper M 9_upper_M upper M 10_upper_M upper M 5_upper_W upper W 6_upper_W upper W 7_upper_W upper W 8_upper_W upper W 9_upper_W upper W 10_upper_W upper W

TESTS FOR DIFFERENCES BETWEEN section GROUPS (across all stratum groups) Global Test Sample statistic (Global R): 0.615 Significance level of sample statistic: 0.1% Number of permutations: 999 (Random sample from a large number) Number of permuted statistics greater than or equal to Global R: 0

Pairwise Tests R Significance Possible Actual Number >= Groups Statistic Level % Permutations Permutations Observed lower, middl 0.519 0.1 213444 999 0 lower, upper 0.844 0.1 213444 999 0 middl, upper 0.494 0.1 213444 999 0

TESTS FOR DIFFERENCES BETWEEN stratum GROUPS (across all section groups) Global Test Sample statistic (Global R): 0.105 Significance level of sample statistic: 5.4% Number of permutations: 999 (Random sample from 98611128) Number of permuted statistics greater than or equal to Global R: 53