Table S1. Predicted Amino Acid Sequences of the Putative Venom Compounds and Other Peptides

Table S1. Predicted amino acid sequences of the putative venom compounds and other peptides from the venom glands cDNA library of Mesobuthus gibbosus.

Name Sequence Match / % Identity E-value

Toxins-like

MgibC5 MKRFSKIICYVLILTLMTVIFSDTLVDAVDCDVDECDTECKARGYSKGTCHDFNDIGCKCHKYS* Potassium channel toxin alpha-KTx 10.1 0.034 [Centruroides noxius], 34%. 6 Cys MgibC10 MKLLLLLIVSASMLIEGVVNVGDGYIRMRDGCKVSCVWGDEGCRQECVAYGASYGYCWTWGLGCWCEGL Depressant insect toxin BmK ITa1 1e-41 PDDKIWKSESNTCGGKK* [Mesobuthus martensii], 80%. 8 Cys Mgib2 MSGLSVFILIALVLSVIIDVLNNSKVEAACKENCRQYCQAKGARNGKCINSNCKCYY* Potassium channel blocker alpha-KTx 26.1 3e-18 [Mesobuthus martensii], 70%. 6 Cys Mgib3 MNRFFILLLLIVILSHAKAEDESYRGNCPSLKKPCNSDRDCCPYGEKCLSAGAGYYCKPDPGP* Calcium channel toxin BmCa1, 58%. 6 Cys 7e-15 Mgib13 … Sodium channel toxin-4 [Mesobuthus 3e-40 FDDGYPVKNGCRISCIPDEHEDLCNQFCKKNKANSGECDFDADACKCWGELDGMEIWNPKSSECKSWND eupeus], 85%. Partial gene, 8 Cys NLITKILEN* Mgib23 MKFLFLTLVLLYFTAILVFIVFPSYAQIQTNASCTTSTHCVEPCRKRCLLIHKCINDKCTCYPRINICEKKNN* putative potassium channel toxin Tx771 1e-14 [Buthus occitanus israelis], 57%. 8 Cys Mgib24 MQRNLVVLLFLGMVALSSCGLREKHFQKLVKYAVPEGTLRTIIQTAVHKLGKTQFGCPAYQGYCDDHCQDIK Potassium channel toxin BmTXK-beta-2 2e-59 KQEGFCHGFKCKCGIPMGF* [Mesobuthus martensii], 99%. 6 Cys

Mgib29 MKIFFAILLILAVCSMAIWTVNGTPFAIRCKTDSDCSYKCPGNPPCRNGFCKCT* Potassium channel toxin alpha-KTx 14.2 1e-27 [Mesobuthus martensii], 91%. 6 Cys Mgib49 MKLLLLLIVSASMLIEGVVNVGDGYIRTRNGCKVSCVWGNEGCRQECVAYGASYGYCWTWGLGCWCEGLP Depressant insect toxin BmK ITa1 5e-42 DDKIWKSSTNTCGGKK* [Mesobuthus martensii], 80%. 8 Cys Mgib88 MKFLYGIVFITLFLTVMIATHTEAAMCMPCFTTNLNMEQECRDCCGGTGRCFGPQCLCGYD* venom chloride channel toxin-1 [Mesobuthus 6e-24 eupeus], 83%. 8 Cys Mgib113 ...FIVFPSYAQIQTNASCTTSTHCVEPCRKRCLLIHKCINDKCTCYPRINICEKKNN* putative potassium channel toxin Tx771 1e-08 [Buthus occitanus israelis], 54%. Partial gene 8 Cys Mgib248 MYYFVMIIFALLMTGVKNESWDFLAGKCSCLLRCPTKARCNGFCLDSGAKSGRCQRVKGVDYCICEDLPVK Sodium toxin peptide BmKTb' [Mesobuthus 5e-07 TMKEDLRICTPAFSD* martensii], 44%. 8 Cys

Antimicrobial and Cytolytic

MgibC1 MQLKKQLIVIFFTYFIVVNESEAFFGALFKLATKIIPSLFRKRKGREVMMKRDLEQLFDPYQRNLELDRLLKQLP antimicrobial peptide marcin-18 [Mesobuthus 4e-33 NY* martensii], 81%. MgibC6 (ORF1) …WDTCWHPLYKMKVVFIIMLLVLACCINENLVDARCPFSSSRCVLHCRDNGFGSGKCKWFKCRCLKKKK* defensin [Medicago truncatula], 31%. Partial 0.29 gene 8 Cys MgibC8 MSSKTLLVLLLVGVLVSTFFTADAYLASMDFDNDALEELDNLDLDDYFDLEPADFVLLDMWANMLENSDFD Non-disulfide-bridged peptide 6.2 9e-08 DDFE* [Mesobuthus martensii], 93%. MgibC9 MNKKTLLVIFFVTMLIIDEVNSIRWGSLFKRVWKSKLARKLRSKGKSLLKDYANRVLSGGPEEEAAPPAERKR Bradykinin-potentiating peptide NDBP6 2e-13 * [Lychas mucronatus], 85%. Mgib253 MSSKTLLVLLLVGVLVSTFFTADAYPASMDFDNDALEELDNLDLDDYFDLEPADFVLLDMWANMLENSDFD Non-disulfide-bridged peptide 6.2 1e-26 DDFEY* [Mesobuthus martensii], 94%.

Other venom components

MgibC11 MTLTNGLSLRTIFITLLLLLPPHLLATTASITKIQTRNRTRKVRGFILLSEAAENRD* venom protein Txlp2 [Hottentotta judaicus], 1e-12 79%. Mgib223 MKRFLVFSILFQTVFCMKTSDQVGIITYQGVPRRERKCILGPWIHLEDGSVFHDSDRCEIKTCHITAQKAYLE venom peptide [Hottentotta judaicus], 28%. VQSCRYKVNCERQILEPYFPHCCPTSPKCT* 8 cys 5.8 Mgib277 ...WCGAGSSAKHEDELGRFNDTDSCCRHHDHCHDNIKGKETKYGLKNKDSTTMSHCDCDEEFYACLKTVD phospholipase A2D precursor [Tribolium 1e-37 SIISNNVGNMFFNFLQKKCFREDYPIKRCVKKSLIRRRCKKYELDRTKPKIWQIFDPKEY* castaneum], 49%. Partial gene. 10 Cys

Cellular Proteins

MgibC6 (ORF2) MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRIDRLLGNRHLHKER transposase of Tn10 [Shigella flexneri 2b], LAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADL 100% ASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYK RLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIRTPKQLVNIYSKRMQI EETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTV RLGMEVLRHSGYTITREDSLVAATLLTQNLFTHGYVLGKL* MgibC7 MKLILLLIVMGILAISKCSVGNTLCELSRNQKIVLLECLESYLTEEQKASEYQFYQCLGYDSVIDYYEEICGLSE ribonuclease R [Coxiella burnetii RSA 331], 0.61 KEQEELHLSYIKCHNEMTPYSGSATDEDARNCLNNAIESEQ* 33%. Partial gene Mgib18 MATTSKPEDHRKKWDRDEFERLAHERILEEYELEKKAKDKQPPIRREYLKPRDYRVDLESKLGKTTVITKTTP zinc finger matrin-type protein 2-like [Oryzias 3e-89 ASQAGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSTLEQVKKRFEMNRKKLDEKKKEYDFE latipes] Actinopterygii, 69%. QRMQELRDEEEKLKEYRRERRKERKRKCQDSRDDDIDPEMAAIMGFSEFGSSKK* Mgib26 … NADH dehydrogenase subunit 3 [Mesobuthus 3e-43 VLLLVLVFRILLRSIILFVGGVISKKLEVEKEKGSVYECGFESRKRARVPFSLQFFMVGVIFLIFDVEIVLIMPVP gibbosus], 90%. LEAWLRGGEVLLFFFFVFLLLFGLFFE* Mgib36 …MLISKIIKRIDWFPSVFKYFYRSERGYRKGLRRKPISVISENKNEDAKQIFKRDIMENLKQE... Monogalactosyldiacylglycerol synthase, partial 4.1 [Megasphaera sp. NM10], 43% Mgib104 GGILRNLHLNGARIFFVCLYFHIGRGIYFGSFKFYFTwITGVVIFLLTIITAFLGYVLPwGQMSFwGATVITNLV cytochrome b [Mesobuthus gibbosus], partial, SAVPYIGSEVVQWLwGGFSVDNPTLTRFFSFHFLLPFVILGRVIIHLIFLHERGSRNPLGVKRDFDKIPFHPYF 95% SLKDLLGALFLIIFLIFISLLFPNFLSDPENCIPANPLVTPVHIQPEwYFLFAYAILRSIPNKLGGVIALIMSVLILIL LSFSTSKSRAFSFRIGSRSLFwILVNIFILLTwIGARPVEFPYIMLGQGLS Mgib142 MGIVTVHKKKSNLKMNKGTMFLSFILILLIVDGIKSYNIEREEELEERLLDDDLKEELEENRNELRQKRDRYLP Adhesive plaque matrix protein, partial [Bos 2e-17 TVQKRERYLPTVQKRERYLPTVQKRERYLPTVQKRERYLPTVQKRERYLPTVQKRERYLPTVQKRERYLPTVQ grunniens mutus], 20% KRERYLPTR* Mgib263 EST MPPKQDQKKKDTKGTTKKKEGASSGGKAKKKKWSKGKVRDKLNNLVLFDKATYEKLLKEVPSYKLITPSVV putative 40S ribosomal protein S25 6e-51 SERLKVRGSLARRALEELRQKGLIKQVVKHHSQIIYTRTTKADDST* [Dolomedes mizhoanus], 87% Mgib264 MSWQAYVDNQICAQVSCRLAVIAGLQDGAIWAKFEKDMPKPVTQQELKLIADTMRTNPNSFTESGIRLGED Blo t profilin allergen [Latrodectus hesperus], 2e-76 KYYCLHAENSLLRGRKGSSALIVVATNTCLLVAATTDGFPPGQLNAVVEKLGDYLRSNNY* 84%

Unkown (hypothetical)

MgibC3 GVCSTFKIFHDLSSNRCKPGWFLS hypothetical protein 11, partial [Urodacus 7e-05 yaschenkoi], 92%. MgibC4 GVCSTFKIFHDLSSNRCKPGWFLS hypothetical protein 11, partial [Urodacus 6e-05 yaschenkoi], 92%. Mgib1 MKNLAIITLSLISLSTSFSLFPSLSLPSVPLYPSSLIPLFMKDPVKEITKIYCVLKNVNIARSCARENNQGKISDF hypothetical secreted protein [Hottentotta 4e-32 FRKCMQKITSLKTLDEMRDFYCNKMNLEETFKAKACLDPGIMNMVLTDATFVPTILNCLFKAKNSEK* judaicus], 45%. 6 Cys Mgib45 MKPVQSVVLFLLLLALHASLSARIARQAEDLDDENNEVPQDEDSALDPDEDSEEDDDDGFSFRFSWNPFQN hypothetical secreted protein [Hottentotta 3e-42 FPDIFKQMRDNMNWIYSNLFNETSNLPEVYKNVSSEIVTIGGSKFNVSKTIIKKADNNSQVVISSVSMDQVN judaicus], 77%. KKKK* Mgib72 EST …SSSLPEAVLSVFRLLPLSSISLTLRFTVILRTSGITSHKTCYPDVPKMPGTIKKTSLLALCP* hypothetical protein [Plasmodium berghei 0.070 strain ANKA], 46%. Partial gene Mgib95 MMESLSCVGLSYALIEYIVNSDLSLFYPQLKSCLFLASCEECILDVESYCSFSPPNKSVAVKEHVLAACKIYVE conserved hypothetical protein [Ixodes 6e-69 GREGVIILDPGYHVGIPIVAMKDGLYPHKGWFVQSETPKSKKEYCYQLKGDYVHWTVRETRNGKEETWCNL scapularis], 42%. IYIKKKFLSYISVSEKRNLVFNFRTLVARDEKQPVGGFYCNLEGDEKFTLFFKDYLGKRIEIKIPFGYFKGSRNN NEYESAIRKCAVQIKTNSALLVGMMTQLVEAYYDKEFMLPVNEINREIDED* Mgib99 EST … hypothetical protein CAPTEDRAFT_188127 2e-05 EEICHMLTKYIKLRKLPELFSKLFASLENNSTEKNLIYPAEFLQDLSSQISYLPAGQILEIWKICLVNGSKLLNDI [Capitella teleta] Polychaeta, 25% . Partial KKSKNEISIKCLNMIAEILRIFILHFKIDRDICLSSKKQTELENITVFIKKFMNICIKKNIQADLGYSILSLCYVW gene GELHLSMKHYNRFYQFKIEFTKEDKGLFDLNAIHYYFTSQEWTDLLNNFENIKENKIQY… Mgib222 MKISIALVLGMIACSFVCINGGDFDQYCSIPSNIRELVWNCIYIYLPQSEKTVFSQMSQCFGVSTFDKVINQF hypothetical protein [Pandinus cavimanus], 5e-05 CGLSDDEQETVALQHEACLSEIDFDNFHGPTVSQYQTQSCIQRRLG* 27%. Mgib267 EST …VPFFFSGIFFFEHAQFLHYQVFKKMFHSHISIMNIFW* hypothetical protein [Vibrio splendidus]. 0.53 Partial gene

No Match

Mgib55 EST MVISYLYFLFEIFLEGSVKRGGGVFFGGVFENYVMGGREPLFAGKVALCCFKKNFFSVF* Mgib16 EST MGGVSPLLAPPVFPFGVPVFFFGGIWGGKNFPPGNPFAPVFPPVPPPRGGSWGSPPGGPTRGPGGGPSFPR GAKFCGSLRTPPKNFF*