2.Demonstrate the Proper Use of a Light Microscope

Schoolcraft College

BIOL 243 COURSE SYLLABUS 1.0 WIDS: Spring 2012

Instructor: Dr. Wilson Muse Course:

BIOL 243 Microbiology Section: 125353 (3 - 4) 4 Cr. Hrs.

Text: Meeting Days/Times: M, T, W, Th Microbiology: Principals and Explorations by Black 7th edition, Wiley 2008 6:00 pm – 7:26 pm Lecture 7:30 pm – 9:37 pm Lab Microbial Applications: Laboratory Manual in General Microbiology by Benson 11th edition (short version), McGraw-Hill 2009 Room: BTC 200 (lecture) Additional Materials: BTC 210 (lab)  Labcoat required available at bookstore  Scantron Testing Forms (#882) 50 questions/side  Sharpie marking pen : blue or black Instructor's Office: BTC500 Course Description:

Instructor's Office Hours: This course covers the world of microbes including microbial structures and function, By Appointment only biochemistry, metabolism, genetics, control of microbial growth, infectious diseases, immunity, classification and epidemiology. Laboratory techniques commonly utilized in microbiology are introduced, including: microscope use, bacterial smears, staining Instructor's Office Phone: methods, aseptic techniques, isolation of pure cultures, identification of unknown Call main office leave message microorganisms, and antibiotic testing. 734-462-4400 ext 5226 Prerequisites:

Instructor's E-mail: BIOL 101. [email protected] Core Abilities:

Non-Discrimination Policy: Core abilities are broad outcomes or skills that every graduate of Schoolcraft College is It is the policy of Schoolcraft College expected to achieve. These skills go beyond the context of a specific course or program, that no person shall, on the basis of are important in every area of learning, and are the skills employers and other race, color, national origin, gender, stakeholders indicate are essential. Schoolcraft College requires candidates for all age, marital status, creed, or associate degrees to demonstrate competency in eight core abilities: Communicate disability, be excluded from effectively; Think creatively and critically; Use technology effectively; Use participating in, be denied benefits of, mathematics; Manage information; Work cooperatively; Act responsibly; and or be subjected to discrimination Demonstrate social and cultural awareness. during any program or activity or in employment. The following core abilities have been identified as important in this course:

1. Think creatively and critically 2. Use technology effectively BIOL 243 WCSA 09/11 3. Work cooperatively

1 Competencies:

Upon successful completion of the course, the student should be able to:

1. Summarize the major events associated with the history of microbiology.

2. Demonstrate the proper use of a light microscope.

3. Prepare slides for microbiological examination.

4. Apply aseptic techniques for the transfer and handling of microorganisms and instruments.

5. Demonstrate proper use of microbiological media and test systems including isolating colonies, maintaining pure cultures and using biochemical test media.

6. Operate standard microbiology laboratory equipment correctly.

7. Apply safety, protective and emergency procedures in a microbiological laboratory.

8. Identify the key characteristics of the major groups of organisms studied in microbiology (bacteria, algae, protists, fungi and viruses).

9. Explain normal flora.

10. Explain several ways in which microbes affect our lives.

11. Identify the significance of Koch's Postulates.

12. Contrast cell walls of gram-positive and gram-negative bacteria.

13. Differentiate bacteria and viruses.

14. Compare the various metabolic pathways in microbes.

15. Explain the purpose of Bergey's Manual.

16. Explain the process of DNA replication.

17. Explain protein synthesis including transcription, RNA processing and translation.

18. Explain how genetic mutation and recombination provide material for natural selection.

19. Outline the steps, mechanisms, and applications associated with genetic engineering.

20. Compare the bacterial chromosome and plasmid DNA.

21. Explain the function of the DNA operons with specific examples.

22. Summarize important diseases caused by microorganisms (etiological agents) and their mode of transmission.

23. Classify viruses based on their nucleic acid type and structure.

24. Explain nosocomial infections and their importance.

25. Evaluate the various physical and chemical methods used to control microbial growth.

26. Characterize the various host-microbes interactions. 2 27. Contrast innate and adaptive defenses.

28. Compare the origin and differentiation of the B-cells and T-cells in the human body.

29. Examine the factors and mechanisms involved in immune disorders and hypersensitivity.

30. Outline the use of microorganisms in environmental and industrial applications.

31. Identify basic epidemiological techniques and their applications.

32. Investigate the role of microorganisms in plant growth and nutrient recycling.

Course Specific Information:

Students will participate in four hours of laboratory study each week. Students will be using a microscope and other equipment to perform experiments to study the structure and function of microorganisms.

There will be sufficient time to complete the experiments and laboratory exercises during the laboratory period.

Students will be evaluated on their laboratory technique and class participation.

There will be no make-up labs available.

Students that participate in the laboratory exercises must at all times wear an approved lab coat, closed toe shoes and abide by the laboratory safety protocol guidelines distributed by your instructor.

All Biology Laboratory Classes  Lab coats are required.

 Food or beverages are not allowed in the lab.

 Long hair is to be tied back.

 Open toed shoes and “dangling” jewelry are not to be worn.

 Splash resistant safety goggles are to be worn by everyone in the lab when dissecting, handling or heating chemicals/liquids.

 Gloves are to be worn for all dissections and when requested by the instructor.

 Never place any laboratory supplies, pens, papers, etc., in your mouth.

Preparing Laboratory Work Space  Familiarize yourself with the location and operation of all safety equipment including the safety shower, eyewash sink, first aid kit, broken glass box, and fire extinguisher.

 Familiarize yourself with the bio-hazardous waste disposal process and the location of bio-hazardous waste receptacles.

 The Laboratory work space/ bench should contain only materials necessary to complete the experiment.

 Store all coats, books, and book bags as directed by the instructor.

During the Laboratory Class  Laboratory accidents (injuries, spills, safety hazards, damaged equipment, etc.), no matter how minor are to be reported to the instructor immediately. 3  Use caution when heating liquids.

 Handle hot glassware with tongs.

 Turn off gas burners or other heating surfaces when not in use.

 Wash hands when you come in contact with any reagents and when you need to leave the room.

 Should you need to leave the laboratory, secure your lab materials and as you exit the room make sure the door closes.

End of Lab - Cleaning Laboratory Work Space  Return all reagents and equipment to the laboratory cart or designated storage space at the end of the class.

 Clean and store microscopes as directed by your instructor. Always place microscopes in the microscope cabinet with the lowest power objective lens in the viewing position.

 Dispose of live cultures as directed by your instructor.

 Dispose of bio-hazardous waste in appropriately marked receptacles.

 Place all scraps of paper, paper towel, etc. in the wastebaskets, not in the sink or on the floor.

 Wash hands before exiting the lab.

A website (http://www-personal.umich.edu/~muse/Biol243.html) is available for class study materials as is a class Blackboard

Methods of Instruction: Any combination or all of the following may be used in this course.

Delivery of Course Content: Lecture, Electronic Presentation, Demonstrations, Video/Audio Presentations, Case Studies, Field Trips, Website(s), Course books, Articles, Other Resources

Assessment: Practical Application, Quizzes and Tests, Papers and Presentations, Discussion, Other Projects

Communication: Lecture, Collaborative Discussion, Group Projects, Electronic Communication, Course Website

Student Responsibilities: 1. As a college student, you are expected to schedule at least two hours of study time outside of class for each hour the class meets.

2. You can expect that questions related to homework will be answered at each session.

3. You are responsible for reading the text and not relying entirely on the instructor presenting every detail.

4. You must show the attempted work when seeking help in completing an assignment. You are encouraged to contact the instructor during posted office hours or by special appointment.

5. You may not always be able to complete assignments during the scheduled class session. Therefore, extra time outside of class must be allowed to complete these assignments.

6. You should save (archive) samples of your best work because an electronic portfolio may be required for program completion. Contact your instructor if there are any questions. 4 7. You are responsible for all announcements and material presented in class, for completion of all homework and lab assignments, and for actively participating in the learning process.

8. If you plan to e-mail your instructor with questions or to leave a message on your instructor's voice mail, identify yourself and the class you are referring to before beginning your message or question. Be sure to leave a number for a return call.

9. If you have any health conditions that might create a classroom emergency (i.e., seizure disorders, diabetes, heart conditions), you are responsible for informing your instructor.

10. If you have any needs for test taking or other accommodations, you are responsible for discussing them with your instructor. The instructor must have received notification from the Learning Assistance Center.

11. You should plan to attend all class sessions. Class attendance contributes significantly to academic success. Students who attend classes regularly tend to earn higher grades and have higher passing rates in courses. Excessive absences may jeopardize grades or even the ability to continue in the course.

Faculty Responsibilities:

1. Assignments will be graded and returned to the students within one week.

2. Exams and other major assessments not returned to students will be kept on file for a minimum of three months after the semester ends.

3. Courses will be taught in accordance with the description published in the catalog.

4. A Course Syllabus will be distributed at the first session of each class.

5. Timely feedback will be provided to students regarding their progress.

6. Faculty will make themselves available for student consultations and will respond to emails, phone calls, and student contacts in a timely manner.

7. Attendance will be taken and reported to the Registrar's Office for State and Federal Report purposes.

Grading Rationale: 1. Four exams worth 100 points each will be given based on readings, lectures, and handout assignments.

All exams are objective: multiple choice, true/false, matching, short answer, fill in the blanks and will not be cumulative.

2. There will be two lab exams each worth 50 points. These exams will consist of fill in the blanks and short answer questions. Lab tests will be lab practical style. Note: As a general rule, no make up exams will be available! Specific cases of missed exams will be resolved on an individual basis and require appropriate documentation of excuse.

3. Each week two laboratory periods will be held. Students will keep a laboratory notebook which will be graded periodically throughout the term (50 pts). Students will identify an unknown organism and be graded on their laboratory technique and class participation (100 points). No make up labs will be available.

4. The lab section will have a series of performance-based competencies you must master in order to get all the participation/lab points. The list of skills you must demonstrate will be posted in the lab section. (gram stain, colony isolation, spore stain etc.)

5. Several homework assignments will be given and graded during the term (50 pts)

5 Exams will be retained on file for a minimum of 2 months after the semester ends.

Missed or Late Assignments/Assessments:

Students are expected to plan their schedule so that they can be present for scheduled class sessions and manage their time so that assignments and assessments can be completed on or before the date they are due. Make-up of a missed exam or acceptance of a late assignment is at the discretion of the instructor.

Academic Integrity:

In accordance with the Schoolcraft College Student Code of Conduct, students are prohibited from engaging or participating in acts of dishonesty, including but not limited to cheating, plagiarism, or other forms of academic dishonesty. The complete Student Code of Conduct is available in the catalog and at: http://www.schoolcraft.edu/academics/policies/conduct.

Any academic dishonesty associated with assignments or exams in this course will result in the grade of zero and may lead to further disciplinary action.

Determination of Final Grade: Example of Final Grade Computation:

Earned Possible Exam 1 92 100 Exam 2 86 100 Exam 3 81 100 Exam 4 79 100

Homework assignments 49 50

Identification of Unknown Organism 78 100

Lab Assessments 1 and 2(50 points each) 85 100

Lab technique/participation 45 50

Laboratory notebook 45 50

Total 640 750 pts

Calculation: 640 ÷ 850 = .853 Final Grade = 3.5

Grading Scale 100-90 4.0 74 2.4 89 3.9 73 2.3 88 3.8 72 2.2 87 3.7 71 2.1 86 3.6 Average 70 2.0 Excellent 85 3.5 69 1.9 84 3.4 68 1.8 83 3.3 67 1.7 82 3.2 66 1.6

6 81 3.1 Below Average 65 1.5 Very Good 80 3.0 64 1.4 79 2.9 63 1.3 78 2.8 62 1.2 77 2.7 61 1.1 76 2.6 Poor 60 1.0 Good 75 2.5 Unacceptable 59 & Below 0.0 Students who do not officially withdraw from this course, have a score that falls in the unacceptable range, or do not take the final exam will receive a final grade of 0.0.

Class Cancellation: Classes canceled on a one-time basis during the semester due to instructor illness, etc. will be listed on the Schoolcraft Web Page. Students may click on www.schoolcraft.edu for more information. For College closings due to weather or other emergencies, listen to major area radio or TV stations for updates.

Additional Services:

The Learning Assistance Center may have tutors for this course. The LAC is located in the Bradner Library Room 119. The direct number is (734) 462-4436. The LAC is also located at Radcliff Center, RC 120. The number is (734) 462-4400, ext. 6021.

There are general (open) computer labs for student use both at Livonia and Radcliff. In Livonia, MC100 (lower level of the McDowell Center) is open daily and on weekends with hours posted in the lab. At Radcliff, RC140 will have weekly hours posted outside the room. Call (734) 462-4400, ext. 5529 for hours at both campuses.

Lab technicians are available in MC-100 to help you with hardware or system problems in the labs. They are not tutors for various software packages.

Food and drink are not allowed in any computer labs. Additional policies may be posted in the computer labs.

Classroom Etiquette: For your benefit and for the benefit of your classmates: - At all times be considerate to your classmates and to your instructor. - No food or beverage is allowed in any classroom. - Come to class on time, ready to ask questions about previous lessons/assignments. - Ask pertinent questions; contribute to discussions; avoid "private" conversations that distract the instructor and other students. - Remember that the faculty member is the one to end the class - do not prepare to leave early. - Cell phones must be turned completely off during class time.

Keys to Success: - Have a positive attitude about learning and the class. - Attend all class sessions and be punctual. - Read the textbook and the handouts before the session and again after. - Do your own lab projects. Work with your "study partner." Ask for help when needed. - Don't expect to understand every topic the first time it is presented; review often and spend as much time as necessary to master the material. - Enjoy the class!

Important Dates:

Final Exam: ______7/2/12__July 2nd______

7 Last Day to Withdraw: ___ 6/17/12 Sunday June 17th______

Holidays: Memorial day (no classes) - Monday May 28

Important dates for registration, semester start and end, withdrawal from classes and holidays for each semester may be found by selecting the academic calendar link at www.schoolcraft.edu

Overview Schedule

NOTE TO STUDENTS: The basic topics in this course must be covered but may be accomplished using a modified version of the schedule listed below. An online version of the day to day class syllabus will be available online: http://www-personal.umich.edu/~muse/Biol243.html

Session Topics Chapters Assignments/Assessments Due Date Aug 31 Introduction to Microbiology 1 Prokaryotic and eukaryotic cell 2-4 structure and function Microcopy and staining techniques 3, 4 Growth and culturing of bacteria 6

2/13 Exam 1 Ch 1-5 Metabolism of microorganisms 5 Microbial genetics 7 Introduction to taxonomy: 9, 10 prokaryotes and viruses

3/14 Exam 2 Ch 6-9 Eukaryotic microorganisms Control of microorganisms 13,15 Interactions between microbe and 14 host

4/11 Exam 3 Ch 10, 12-15 Basic principles of immunity 17 Infectious diseases of humans 19-24 Environmental and applied 25 microbiology

8 May 2 Exam 4 Ch. 16-25

BIOL 243 CSA Muse Spring 2012

There will be no make-ups available for missed labs.

Muse’s Biology 243 (Microbiology) Lecture Schedule Spring 2012

Week Date Topic Chapter 1 5/14 M Overview and History of Microbiology 1 5/15 T Review of Chemistry: Bonds, pH and polarity 2 5/16 W Review of Chemistry : Redox, organic chemistry 5/17 Th Microscopy and Staining 3 2 5/21 M Characteristics of Prokaryotic and Eukaryotic cells 4 5/22 T Essential Concepts of Metabolism 5 5/23 W Metabolism part II 5/24 Th Exam I (ch. 1-5): Growth and Culturing of Bacteria 6 3 5/28 M memorial day-no class - 5/29 T Growth and Culturing of Bacteria: part II 6 5/30 W Intro to DNA. Molecular Biology 5/31 Th Microbial Genetics 7 4 6/4 M Gene Transfer and Genetic Engineering 8 6/5 T Microbial Taxonomy (Bacteria) and Genomics 9 6/6 W Viruses: Bacteriophage - (Review ch6-9) 10 6/7 Th Exam II (Ch 6-9): Viruses part II Plant and Animal Viruses 10 5 6/11 M Viruses part 3: Retroviruses, (HIV) 10 6/12 T Sterilization and Disinfection 12 6/13 W Antimicrobial Therapy and antibiotic resistance mechanisms 13 6/14 Th Host-Microbe Relationships and Disease Processes 14 6 6/18 M Disease pt 2: Epidemiology and Nosocomial Infections 15 6/19 T Exam III (Ch 10-15) 6/20 W Nonspecific Host Defenses 16 6/21 Th Adaptive Immunity and Vaccines 17 7 6/25 M Immunological disorders and Immunological biotechnology 18 6/26 T Disease causing microbes 19-22 6/27 W Environmental microbiology I: (decomposers, symbionts) 25 9 6/28 Th Environmental microbiology II: niches and adaptation 25-26 7/2 M Final exam (#4) (16-26) Final reports due Final: Exam #4 July 2nd

Text: ISBN 978-0-470-10748-5 Microbiology: Principles and Explorations 7th ed. Jacquelyn G. Black 2008 John Wiley and Sons

Muse’s Biology 243 (Microbiology) Lab Schedule Read the lab exercise before the scheduled date to get an idea of what we are doing

Week Date Topic chapter/exercise 1 5/14 Brightfield Microscopy - Protozoa, 1, 5 5/15 Algae, and Cyanobacteria, The Fungi: Yeasts and Molds 5,7 5/16 Aseptic Technique 8 5/17 Ubiquity of Bacteria 6 2 5/21 Smear Preparation; Simple Staining 10,11 5/22 Gram Staining 14 5/23 Spore Staining: Two methods, 15 5/24 Acid Fast: Ziehl-Neelson Method 16 3 5/29 Capsular Staining, 13 5/30 Motility Determination 17 5/31 Pure Cultivation 9

4 6/4 Cultivation of Anaerobes 20 6/5 Enumeration of Bacteria: The Standard Plate Count, 19 6/6 Lab exam #1 pH and Microbial Growth 26 6/7 UV lights: Lethal Effects 28 5 6/11 Temperature: Lethal Effects 25 6/12 Evaluation of Alcohol: Effectiveness as a antiseptic 30 6/13 Antimicrobic Sensitivity: The Kirby Bauer Method 31 6/14 Microbiology of Alcohol Fermentation–unknown dispensed 50 6 6/18 Morphological Study of Unknown Bacteria 34 6/19 Cultural Characteristics 35 6/20 Physiological Characteristics: 38 6/21 Ox and Fermentation tests 36 7 6/25 Physiological Characteristics: Hydrolytic Reactions 37 10 6/26 Physiological Characteristics: Biochemical Tests 38 6/27 Identification of Unknown Bacteria/ Bergey’s manual 39 6/28 Application of specialty test media 43 8 Turn in final lab report - 7/2/12 Final lab exam

Lab Text: ISBN # 978-0-07-352254-8 Benson’s Microbiological Applications: Laboratory Manual in General Microbiology (Short Version) 11th Edition Albert E. Brown 2009 McGraw-Hill

Due to the nature of the live materials used we must be somewhat flexible on what days actual experiments are run. This schedule is subject to minor changes. The instructor will keep the class updated as to which experiments will be run on a day to day basis.

There will be no make-ups available for missed labs.