Subject: MEDICAL BIOLOGY - 2011. /12. Academic Year

University of Split School of Medicine Department of Medical Biology Subject: «MEDICAL BIOLOGY» - 2011. /12. Academic year Program: lectures (L=34 h), seminars (S=34 h) and practical courses (P=32 h).

1. CELL BIOLOGY AND MEDICINE. Molecular medicine. L - Cell structure and function. Prokaryotes vs. Eukaryotes. Cell chemistry. Macromolecules. S - Cell research methods. Stucture and ultrastructure. P - Microscopy. Using a microscope. Visual field size. Sample preparation for microscopy /demonstration/. Sample slides (prepared and wet-mount). (Microscopy room= M). Slides: 1. arrow 2. cork cells 3. frog blood cells

2. Cell structure and function . THE NUCLEUS. L - The nucleus. Central dogma of molecular biology. S - Nucleus. Prokaryotic vs. Eukaryotic cell. P - Nucleus in prokaryotes and eukaryotes. Cell size measurement (M). Slides: 1. Prokaryotes: different types of bacteria 2. Eukaryotes: human tongue squamous cells 3. Cellular fragmentation and centrifugation: animal liver cells

3. MEMBRANE – STRUCTURE AND TRANSPORT. L - Cell membrane structure. Plasma membrane transport principles. S - Human erythrocytic membrane.Biological and artificial membrane transport. P - Human erythrocytic membrane isolation and biochemical analysis (M). Slides: 1. Human erythrocytes in physiological solution 2. Human erythrocytes in hypotonic solution: „white ghosts“ 3. Human erythrocytic membrane: protein and lipid detection 4. Human haemoglobin crystals

4. PROTEIN SORTING AND TRANSPORT. BIOENERGETICS. L - Intracellular membrane organelles. Generation of metabolic energy. S - Endoplasmic reticulum, Golgi apparatus i lysosomes. P - Membrane - transport. Mitochondria (M). Slides: 1. Traube cell 2. Human erythrocytes in hypo-, iso- and hypertonic NaCl solution 3. Human erythrocytes in drinking and sea water 4. Onion cells: plasmolysis and deplasmolysis 5. Rat liver cells mitochondria

5. CYTOSKELETON AND CELL MOVEMENT. L - Cytoskeleton i cell movement. S - Microtubules, microfilaments i intermediate filaments. P - Membrane, intracellular organelles and cytoskeleton (Computer room=CR).

6. The flow of genetic information. NUCLEIC ACIDS - DNA. Human genome. L - Deoxyribonucleic acid - DNA. S - DNA structure and replication. P - Methods of DNA analysis. DNA electrophoresis. DNA detection (Dische reaction). (M).

7. NUCLEIC ACIDS-RNA. L - Ribonucleic- RNA. Transcription. S - Molecular mechanisms of transcription in eukaryotes. P - DNA isolation. (M)

8. DNA - RNA - Proteins. L - From DNA to proteins. Translation. Genetic code. S - Chromosome structure. Eukaryotic chromosome replication. P - Human chromosome and sex chromatin. (M). Slides: 1. Drosophilae melanogaster polytene chromosomes 2. Amphibian oocyte chromosomes 3. Human leucocyte chromosomes 4. Human buccal interphase cell sex chromatin

9. CELL REGULATION. CELL SIGNALING. L - Intracellular signal transduction. S – Fertilization and early embrionic development. P - Gametogenesis, fertilization and early development (M). Slides: 1. Rat sperm cells 2. Rat vaginal cells 3. Sea urchin fertilized oocytes and early stage organism 4. Rat reproductive system isolation and demonstration

10. CELL CYCLE. CANCER. CANCER MOLECULAR GENETICS. L - Cell cycle. Basics of molecular and cancer biology. S - Cell cycle regulation in normal and transformed cells. P - Tumors: teratomas i teratocarcinomas. (M). Slides: 1. Rat embrio: 9 days old 2. Rat fetus : 20 days old 3. Rat fetus and placenta (fetus in toto) 4. Rat experimental teratoma 5. Human teratocarcinoma

11. MEDICAL GENETICS BASICS. L – Classical and molecular genetics. Basic principles of medical genetics. Cell genomes and genes. S - Chromosomes and genes in meiosis in human. Principles of inheritance. Monohybrid inheritance. Monogenetic disorders. Autosomal recessive and dominant disorders in human. Multiple alleles. Problem solving. P - Cell cycle. Interphase i mitosis. Mitotic index (M). Slides: 1. Onion root cells 2. Yeast cells 3. Rat follicular ovarian cells 4. Human cervical cancer cells (HeLa)

12. X-LINKED INHERITANCE. LINKED GENES. L - x-linked inheritance and inheritance of sex. Linked genes and gene recombination. S - x-linked inheritance. Monogenic disorders. Haemophilia. Dihybrid cross. Independent assortment. Problem solving. P - PTC-test (Phenyl Thio Carbamide). Genealogy analysis. Problem solving (M).

13. DNA RECOMBINATION IN BACTERIA AND VIRUSES. L – Prokaryotic and viral genomes. Transformation, conjugation i transduction. Recombinant DNA. S - DNA - RNA - PROTEIN. DNA, RNA and protein analysis methods. P - DNA: PCR (Polymerase Chain Reaction) (M).

14. MUTATIONS. L - Chromosome and genes mutations. DNA repair mechanisms. Polygenic disease in human. S - Mutations and human health. P - DNA: RFLP ( Restriction Fragment Length Polymorphism). Genealogy analysis (M).

15. CLINICAL CYTOGENETICS. L - Human karyotype and chromosomal abberations. S - Karyotype. Chromosome banding methods. P - Human karyotype and metaphase plate formation from leucocytes. Problem solving (M).

16. GENE EXPRESSION REGULATION. L - Genetic regulation in prokaryotes and eukaryotes. Genetic regulation in human. S - Control of gene expression in eukaryotes. P – Gene regulation. DNA - RNA - Protein. Problem solving (CR).

17. MOLECULAR BIOLOGY IN MEDICINE. GENE THERAPY. L - Genomes, transcriptomes, proteomes. Molecular biology and future of medicine. Recombinant therapy. Developmental genetics. Cloning. Ethics and genetics. S – Genetic analysis in humans. Clinical genetics. Genetic counselling. Problem solving.

Teachers: Prof. Tatijana Zemunik, MD, PhD Asst. Prof. Vesna Boraska, PhD Asst. Prof. Ozren Polašek, MD, PhD Maja Barbalić, PhD Marina Pehlić, MD Jolanda Zoković, lab.technician Obligatory literature: 1. Cooper GM, Hausman RE. The Cell, a Molecular Approach. 5th ed. Washington DC, Sunderland (Massachussets): ASM Press, Sinauer Associates; 2009.

Supplementary literature : 1. Cox TM, Sinclair J. Molecular biology in medicine. Blackwell Science, 1997. Oxford, UK 2. Alberts B et. all. Essential Cell Biology, New York, Garland Science, 2/e, 2004. 3. Griffiths AJF et all. An Introduction to Genetic Analysis, 8/e, WH Freeman & Co., 2005. 4. Gelehrter TD, Collins FS, Ginsburg D: Principles of Medical Genetics, 2nd ed. Baltimore, Lippincott Williams and Wilkins, 1998. 5. Tamarin R H: Principles of Genetics, 6e, Boston, McGraw-Hill, 1999. 6. Reynolds A: CD-ROM Genetics - From Genes to Genomes, McGraw-Hill, 2000. 7. Gilbert SF. Developmental Biology, Sinauer, 8/e, 2006. 8. Peruzović M., Zemunik T.: Medicinska biologija, Priručnik za mikroskopske vježbe, Katedra za medicinsku biologiju, Medicinski fakultet u Splitu, Split, 2010.

Subject aims: Understanding the basics of contemporary biological science on molecular level, as per medical student's aspiration and future medical practice.

Subject goals: Student has to demonstrate theoretical and practical knowledge of cell structure and function, developmental biology, genetics and basic principles of molecular biology.

Subject components: Basics in molecular biology (DNA structure, replication, transcription and translation), cell biology (cell structure and function, cell organelles, cell signalling, cell- to-cell communication, cell cycle, apoptosys), developmental biology, genetics (fertilization and early embrionic stage, teratogenesis, human genome, principles of inheritance, gene mutations, population genetics, molecular biology of cancer cells, gene therapy ).

Knowledge and skills students have to obtain: microscoping of prepared and wet-mount biological sample slides; cell analysis by fragmentation and centrifugation; plasma membrane analysis: osmosis, plasmolysis, haemolysis, isolation and biochemical analysis; DNA isolation and electrophoresis; performing and understanding molecular biology methods: PCR and RFLP; conducting an information (biology, genetics) search in available internet databases.

Curriculum: All segments of classes (lectures, seminars and practical courses) are interconnected and mandatory for all students to attend. Students are expected to come prepared to class and to actively participate in pre-assigned discussion topic.

Exams: Students will have written exam at the end of the course where minimum of 60% is required for a positive grade.

WINTER term SUMMER term AUTUMN term 05. 03. 2012. 11. 07. 2012. 05. 09. 2012. 19. 09. 2012.

Medical biology course starts in 31.1.2012. (timetable on www.mefst.hr and notice board).

Teachers of the Department for Medical Biology wishes all the students very successful Medical studies at our School!

In Split, 12. September 2011. Prof. Tatijana Zemunik, MD, PhD Chair of Department for Medical Biology