1 Supplementary Material

2

3 Article title: Symbiotic interactions as drivers of trade-offs in plants: effects of fungal

4 endophytes on tall fescue

5 Journal: Fungal Diversity

6 Authors: Pedro E. Gundel1,2,*, Lucas A. Garibaldi3, Marjo Helander4, Kari Saikkonen1

7 Affiliations:

8 1 MTT Agrifood Research Finland, Department of Plant Production, Jokioinen, Finland.

9 2 IFEVA - CONICET – Faculty of Agronomy, Buenos Aires University (UBA), Argentina.

10 3 Sede Andina, Universidad Nacional de Río Negro (UNRN) and Consejo Nacional de 11 Investigaciones Científicas y Técnicas (CONICET), Mitre 630, CP 8400, San Carlos de 12 Bariloche, Río Negro, Argentina.

13 4 Section of Ecology, Department of Biology, University of Turku, Turku, Finland.

14

15 * Corresponding author: MTT, Agrifood Research Finland, Department of Plant Production,

16 31600 Jokioinen, Finland. Tel: +358 341 881; fax +348 20 772 040; E-mail:

17 [email protected]

18

1 19

20 Table 1. Effect values for minimum adequate models of the influence of endophyte

21 colonization on biomass allocation patterns in Schedonorus phoenix plants from different

22 origins and environmental treatments of water and nutrient addition (see Fig. 4, and

23 Statistical analyses). The coefficients for level of factors are estimated relative to a given

24 level as for example, Water-yes (water added) is estimated relative to Water-no (not in the

25 table). Meaning of terms: Water-yes = water added; Nutrient-yes = nutrient added;

26 Oringin_G = Gotland population; Oringin_K = cultivar KY-31; Oringin_S = Sweden

27 population; Endophyte_E+ = endophyte colonized plants; Endophyte_ME- =

28 manipulatively uncolonized plants; rep_biomass: reproductive biomass; and rep_effort =

29 reproductive effort.

Reproductive Panicles (log10 no. Relative panicle biomass (log10 g) plant-1) number (log10) Fixed effects (mean) Intercept -1.9 1.0 -0.14 Water_yes 0.036 -0.027 0.072 Nutrient_yes -0.49 0.058 0.29 Origin_G -0.11 -0.18 0.098 Origin_K -0.21 0.053 0.32 Origin_S 0.028 -0.11 0.17 Endophyte_E+ 0.081 -0.15 Endophyte_ME- -0.095 -0.011 Origin_G x nutrient_yes -0.33 Origin_K x nutrient_yes -0.39 Origin_S x nutrient_yes -0.089 vegetative biomass 1.31 reproductive biomass 0.67 rep_biomass x origin_G 0.058 rep_biomass x origin_K -0.038 rep_biomass x origin_S 0.094 rep_biomass x endophyte_E+ -0.028 rep_biomass x endophyte_ME- 0.066 reproductive effort -0.60 rep_effort x water_yes 0.092 rep_effort x origin_G 0.18 rep_effort x origin_K 0.28 rep_effort x origin_S 0.12 rep_effort x nutrient_yes 0.25 rep_effort x endophyte_E+ -0.14 rep_effort x endophyte_ME- -0.018 rep_effort x origin_G x nutrient_yes -0.25 rep_effort x origin_K x nutrient_yes -0.37 rep_effort x origin_S x nutrient_yes -0.059

Random effects (standard deviation) Blocks 0.12 0.045 0.040 Plots within blocks 0.13 0.015 0.014 Plants within plots within blocks 0.44 0.18 0.16 (residual) 30 31

32

3