Article Title: Population Structure and Gene Flow in the Endangered Southern Brown Bandicoot

Article title: Population structure and gene flow in the endangered southern brown bandicoot (Isoodon obesulus obesulus) across a fragmented landscape Journal name: Conservation Genetics

Author names: You Li a, b, *, 1, Melanie L. Lancaster a, b, c, Steven J. B. Cooper a, b, d, Andrea C. Taylor e and Susan M. Carthew a, f a School of Earth and Environmental Sciences, the University of Adelaide, Adelaide, SA, 5005, Australia b Australian Centre for Evolutionary Biology and Biodiversity, the University of Adelaide, Adelaide, SA, 5005, Australia c Healesville Sanctuary, Healesville, VIC, 3777, Australia d Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA, 5000, Australia e School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia f Research Institute for Environment and Livelihoods, Charles Darwin University, Darwin, NT, 0909, Australia * Corresponding author. Tel: +86 18609496247

E-mail address: [email protected] (Y. Li).

1 Current address: 701, Jin Ta Xiang 33#, Cheng Guan Qu, Lanzhou, Gansu, 730030, China. Table S1 Sampling information and numbers of pairwise related individuals within each patch (total number of pairwise relatedness comparisons was 10 731). N = Sample size; First-degree relatives = Full siblings or parent-offspring, R ≥ 0.5; Second-degree relatives = Half siblings, R ≥ 0.25.

Patch First-degree Second-degree Patch name N abbreviation relative pairs relative pairs Mount Mclntyre MM 16 8 40

Mc Rosties MR 11 4 18

The Marshes MA 7 2 3

Burr Slopes South BSS 15 23 21

Native Wells NW 15 3 16

Glencoe Hill GH 15 12 38

Long LO 8 2 7

Windy Hill WH 3 1 0

Woolwash WO 7 0 6

Honan HN 8 3 10

Kangaroo Flat KF 6 2 2

Hacket Hill HH 9 8 3

Wandilo WA 16 7 27

Grundy’s Lane GL 11 7 15 Table S2 A summary of P values for the individual HWE tests for each locus in each patch. Significant P values after sequential Bonferroni corrections were denoted in bold.

BSS GH GL HH KF LO MA MM MR NW WA WH WO HN Ioo8 0.071 0.123 0.013 0.417 0.477 1 0.023 0.456 0.282 0.000 0.513 - 0.019 1 Ioo7 - 1 0.142 1 - - - 0.636 0.503 - 0.433 - 1 - Ioo6 0.009 1 1 1 1 0.388 1 0.000 0.001 1 1 1 - 1 Ioo5 0.326 - 0.440 - 1 1 1 1 1 1 1 1 1 - Ioo4 1 0.228 0.021 1 0.460 1 1 1 1 0.181 0.876 0.333 0.029 0.429 Ioo3 0.341 - 1 0.050 0.030 1 1 0.034 - 0.031 0.020 0.198 0.087 0.066 Ioo2 0.058 0.007 0.057 1 1 0.298 0.688 0.006 0.018 0.360 0.164 0.334 1 0.310 B20-5 0.064 0.033 0.010 0.220 0.242 0.001 0.155 0.002 1 0.111 0.007 1 0.209 0.009 B3-2 0.019 0.245 0.725 0.722 0.094 1 1 0.000 0.063 0.057 0.235 - 0.015 0.386 Ioo16 0.247 0.144 0.442 0.026 0.756 0.542 1 0.061 0.103 0.525 0.025 0.196 0.831 0.603 B15-1 - - 0.141 0.126 1 1 - 0.000 0.009 0.119 1 - 1 - B38-1 0.095 0.271 0.179 0.276 0.633 0.648 0.601 0.066 0.504 0.726 0.010 0.467 1 0.729 B35-3 0.012 0.035 1 0.153 0.884 1 0.006 0.056 0.137 0.268 0.067 1 0.776 0.418 B34-2 0.512 0.010 1 0.007 1 0.815 0.020 0.373 0.001 0.208 0.238 0.198 0.776 0.329 Ioo10 0.001 0.012 0.010 0.014 0.072 0.259 0.035 0.821 0.396 0.088 0.491 1 0.549 0.216