Rajiv Gandhi University Of

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA, BANGALORE-560041

SYNOPSIS

DR. RAGHUVEER

FOR THE DEGREE OF AYURVEDA VACHASPATHI

M.D. (DOCTOR OF MEDICINE)

RASASHASTRA

TITLE OF THE TOPIC

“A COMPARATIVE STUDY OF NAVASADARA SATVA, BY TAKING TWO SAMPLES OF NAVASADARA(available source & market sample) AND ITS ANTIMICROBIAL ACTIVITY”

NAME OF THE INSTITUTE

K.L.E. SOCIETY’S POST GRADUATE STUDIES AND RESEARCH CENTRE SHRI B.M.K. AYURVEDA MAHAVIDYALAYA SHAHAPUR – BELGAUM-590003

1 FROM : DR. RAGHUVEER. 1st year M.D. Scholar Department of Rasashastra, K.L.E.S. Shri B.M.K. Ayurveda Mahavidyalaya Post Graduate Studies & Research center Shahapur, Belgaum -590 003.

To : THE REGISTRAR RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA JAYANAGAR, 4TH ‘T’ BLOCK BANGALORE.

THROUGH, The Principal and Head of the Department In RASASHASTRA DEPARTMENT Post Graduate Studies and Research Center K.L.E.S. Shri B.M.K. Ayurveda Mahavidyalaya Shahpur, Belgaum- 590 003.

SUB : Submission of the completed proforma of Synopsis for Registration of subject for dissertation.

Respected Sir,

I request you to kindly register the synopsis on below mentioned subject against my name for the submission of dissertation to the Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore for partial fulfillment of postgraduate course in the specialization of Rasashastra M. D. (Ayurveda)

TITLE OF DISSERTATION:

“A COMPARATIVE STUDY OF NAVASADARA SATVA, BY TAKING TWO SAMPLES OF NAVASADARA (available source & market sample) AND ITS ANTIMICROBIAL ACTIVITY”

Here with I am enclosing the complete proforma of synopsis for the registration of subject for dissertation.

Thanking You, Yours faithfully Place: Belgaum Date:27/11/07 (Dr. Raghuveer).

2 RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE.

PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

1. Name Of The Candidate : DR. . RAGHUVEER And Address 1ST M.D. Scholar (In Capital Letter) Department of Post Graduate studies In Rasashastra. K.L.E’s Shri B. M. K. Ayurveda Mahavidyalaya, Shahapur, Belgaum -3, Karnataka.

2. Name of The Institution : K. L. E. Society’s Shri B.M.K. Ayurveda Mahavidyalaya, Post Graduate Studies and Research Centre, Shahapur, Belgaum – 3 Karnataka.

3. Course of Study And : Ayurveda Vachaspathi M.D. (Ayurveda) Subject In Rasashastra

4. Date of Admission To The Course : 11-06-2007.

TITLE OF DISSERTATION : “A COMPARATIVE STUDY OF NAVASADARA SATVA, BY TAKING TWO SAMPLES OF NAVASADARA (available Source & market sample) AND ITS ANTIMICROBIAL ACTIVITY”

3 5. BRIEF RESUME OF THE INTENDED WORK: -

6.1 NEED OF STUDY: Navasadara is one among the sadharana rasa1, which posseses the properties such as snigdha, sukshma, laghu, teekshna, and ushna2. Similarly Navasadara satva is used in PHUPPUSASHOTHA, AMLAPITTA, HRUDAYADOURBALYA, MURCHA3. As it is indicated in phuppusashotha(Inflammatory condition of lung)i.e. Pneumonia4, the study also extended to carryout antimicrobial activity of Navasadara satva, in the organisms which causes phuppusashotha, such as, Streptococcus pneumococci, Staphylococcus aureus, Klebsiella pneumoniae, E-coli.

6.2 REVIEW OF LITERATURE :-

The grahya Navasadara possesses – Pandura, Lavana, Laghu5

The grahya Khatika possesses – Shewtavarnayukta, mridu, madhura and tikta rasayukta.6

Shodhana of Navasadara: -

1) 1 Part of Navasadara is mixed with 3 parts of jala, kept for some time for sublimation and filter it, then this filtrate is heated to remove jaliyamsha of it. Then it is collected.7 2) By doing urdwapatana process in damaru yantra, the navasadara will get purified, then it is collected from inner surface of upper pot.8

Shodhana Methods of Khatika: - Khatika churna is completly mixed with water and filtered through cloth. Allowed it to remain undisturbed for 3-4 hours, remove the water and collect the material settled at the bottom9.

4 Preparation of Navasadara Satva : 4 parts of Shuddha Navasadara and 3 parts of Shuddha Khatika are taken, and triturated in khalvayantra. Then kept in Damaru yantra, agni is given. The upper part of damaruyantra is condensed by wet cloth, after complete patana allow it to become swangasheeta. Then the Navasadara satva will be collected from inner surface of the upper pot.10 6.3. OBJECTIVES OF THE STUDY : 1. Preparation of Navasadara Satva by classical method. 2. Physico- chemical analysis of Navasadara Satva. 3. Invitro evaluation of its Bacteriostatic and Bactericidal action against the stains of Gram-positive and Gram-negative bacteria responsible for phuppusashotha.

7. MATERIALS AND METHODS :- 7.1 Source of Data :- 1. Samples of Navasadara will be collected from the available source, and Khatika from market, and get authentified by the experts in the subject. 2. The literary data will be collected from the Library, Internet, Medical Journals and Magazines. 3. Physico-chemical analysis will be carried out at well-established laboratory. 4. Antimicrobial study will be conducted in the Microbiology laboratory.

7.2 METHODOLOGY : 1. Navasadara and Khatika having Grahya lakshanas will be collected. 2. Shodhana of Navasadara 7:- 1 Part of Navasadara is mixed with 3 parts of jala, kept for some time for sublimation and filter it , then this filterate is heated to remove jaliyamsha of it. Then it is collected.

3. Shodhana of Khatika 9:- Khatika churna is completly mixed with water and filtered through cloth. Allowed it to remain undisturbed for 3-4 hours, remove the water and collect the material settled at the bottom.

5 4. Preparation of Navasadara Satva by classical method 10: 4 parts of Shuddha Navasadara and 3 parts of Shuddha Khatika are taken, and triturated in khalvayantra. Then kept in Damaru yantra, agni is given. The upper part of damaruyantra is condensed by wet cloth, after complete patana allow it to become swangasheeta. Then the Navasadara satva will be collected from inner surface of the upper pot.

Physico-Chemical Analysis of Navasadara Satva: The Physical changes will be observed before and after shodhana of Navasadara and Khatika and after preparation of Navasadara Satva w.r.t. Organoleptic characters like texture, colour, taste and odour.

Chemical Analysis

- Ash Value - Loss on Drying - Trace Element Analysis

Antimicrobial study 11 :-

Methodology - Microbroth dilution method The micro-organisms will be included to assess the anti-microbial efficacy of the compound. Gram Positive bacteria - Streptococcus Pnemococci / Staphylococcus aureus Gram Negative bacteria - Escherichia coli / Klebsiella Pneumoniae The anti-microbial activity will be carried out by Microbroth dilution method. Here serial dilution of the compound will be tested against fixed density of each bacterium. Results will be expressed as minimum inhibitory concentration (MIC). After overnight incubation, specimen from each tube, which shows turbidity, will be inoculated on blood agar plate. The absence of growth will indicate bactericidal activity of particular concentration of the compound against tested bacteria.

6 7.3 DOES THE STUDY REQUIRE ANY INVESTIGATIONS --- Not required

7.4 HAS ETHICAL CLEARANCE BEEN OBTAINED FROM INSTITUTION --- Not required

LIST OF REFERENCES :

1. Satpute A D, Rasaratna Samuchaya. Choukhamba Sanskrit Pratistana, 2003, 1st Edn 3/115. Page no. 77.

2. Sharma Sadanand, Rasatarangini. Delhi; Motilal Banarasi Das, 1979, 11th Edn 14/3- 4, Page no 326.

3. Sharma Sadanand,Rasatarangini. Delhi; Motilal Banarasi Das,1979,11th Edn, 14/22-23, Page no329.

4. Guyton & Hall, Text book of Medical Physiology, 2000,10th Edn, Page no.488.

5. Satpute A D, Rasaratna Samuchaya. Choukhamba Sanskrit Pratistana, 2003, 1st Edn 3/124. Page no. 79.

6. Sharma Sadananda, Rasatarangini. Delhi; Motilal Banarasi Das,1979,11th Edn, 11/211-212. Page no 279.

7. Sharma Sadananda, Rasatarangini. Delhi Motilal Banarasi Das,1979,11th Edn 14/3-4, Page no 326.

8. Jha Chandrabhushan Ayurvada Rasashastra. Choukambha Bharati, Prakashana. 2003, 1st Edn. Page no 281.

9. Sharma Sadanand, Rasatarangini. Delhi Motilal Banarasi Das, 1979 11th Edn 11/210. Page no 279.

10. Sharma Sadanand,Rasatarangini. Delhi; Motilal Banarasi Das,1979,11th Edn, 14/20-21. Page no 329.

11. Gupte Satish, Medical Microbiology. New Delhi; Jaypee Publishers, 8th [2002] Edn.

7 9. SIGNATURE OF THE CANDIDATE :

10. REMARKS OF THE GUIDE : The present study has not been conducted in any of the research centers. So that the above research work has been selected and recommended for the approval of RGUHS.

11. NAME AND DESIGNATION OF 11.1GUIDE : Dr. P.P. Dindore. M.D. (RS.&BK) Professor Dept of Rasashastra & BK K.L.E’S Sri. B.M.K Ayurveda Mahavidyalaya P.G. Studies & Research Centre. Shahapur, Belgaum-590003.

11.2 SIGNATURE :

11.3 CO-GUIDE : Sri.Gavimath.C.C. Professor Dept of Biotechnology KLEs Engeneering College Udyambag, Belgaum

11.4 SIGNATURE :

11.5 HEAD OF THE DEPARTMENT : Dr. N M HAMPANNAVAR, M D.(RS.&BK) Asst. Prof. H.O.D. Dept. of Rasashastra & BK K.L.E’S Sri. B.M.K Ayurveda Mahavidyalaya P.G. Studies & Research Centre. Shahapur, Belgaum-590003.

11.6 SIGNATURE :

12. REMARKS OF THE CHAIRMAN AND : The Synopsis has been thoroughly PRINCIPAL reviewed by department as well as institutional research committee and hence I recommend it for Registration.

12.1 SIGNATURE :