Jbaids Check Sheet

Revised December 4, 2013

Date:______

JBAIDS CHECK SHEET

USS ______

REF: a) AEL 2-770004381 for JBAIDS Deployment Consumable Items b) System Operation and Verification Test

JBAIDS SYSTEM

Quantity IAW S/U 1. Confirmatory Laboratory 2. Is there a JBAIDS analyzer and laptop onboard? Ref (a) 3. Does the JBAIDS operator have the JBAIDS system manual (Rev.7)? 4. Does the JBAIDS operator have a QA binder/folder and password logs? 5. Is there a JBAIDS repair kit onboard? 6. Analyzer and Laptop: a. Does the laptop boot up without problem and contain applicable JBAIDS software (Latest Version 3.5.1.5)? b. The laptop’s hard drive should be no more than 70% full. (If the hard drive is partitioned, check the drive that the analyzer’s software is running off of.) The laptop should contain no personal information or files that do not directly pertain to the Confirmatory lab. c. With the laptop attached, does the analyzer turn on and perform/pass a Ref (b) self-test? d. Is there a sign on the laptop indicating its dedicated use. Example:”JBAIDS Only” 7. Freezer and Refrigerator: a. Is there a 4°C refrigerator onboard? 1 b. Is there a -20°C manual defrost freezer onboard? 1 i. Is a daily temperature log maintained on the freezer, with the average 1 temperature over the last 3 weeks being no higher than -15°C? 8. Bio-Safety Hood: a. Is there a Class II bio-safety hood installed on the ship, with certification 1 performed within past 12 months? 9. Consumable Supplies: a. Between the “Clean” and “Dirty” areas, does the Confirmatory lab have a minimum of the following critical supplies: b. Mini centrifuge (fits in the lid of the JBAIDS instrument case) 1 c. Vortex Genie 2 Mixer 1 d. Disruptor Genie 1 e. Fisher Digital Dry Bath Incubator 1 f. Ohaus Electronic Balance 1 g. Mini Spin Plus Centrifuge 1 h. P-10-100 ul Pipetman Pipettor 2 i. P-100-1000 ul Pipetman Pipettor 2 j. PickPen, 1-M Magnetic Tool 1 k. PickPen Reusable Tip Box 1 l. Pipettor tips – P10-100 (960/pk) 4 m. Pipettor tips – P100-1000 (576/pk) 2 n. PickPen Tips, Bulk (500/bag) 1 o. Capillaries; 5pks/box 5 p. Capillary capping tool 1 q. Empty Capillary Boxes 2 r. Tube racks (5/pk) 1

For Official Use Only – Distribution authorized to DoD and DoD contractors only. 1 Revised December 4, 2013 s. Phosphate Buffered Saline (PBS) pH 7.4 1 box of 10 t. Triton X-100 (100 ml) 1 bottle u. Gloves, MED 2 boxes v. Gloves, LG 2 boxes w. Bags, biohazard, small 2 packs x. DNA away 1 bottle y. Bleach Towelettes (100/cs) 1 case Ref (a)

Software and Data Storage 1 a. CD/DVD Drive for installing software b. Latest version of JBAIDS software (Version 3.5.0.72 (Feb.2012)) 1 c. External hard drive OR 30 Recordable CDs and CD cases for data transfer 1 d. Printer dedicated to JBAIDS 1

10. Core Reagents Does the lab have a total of 14 different assay kits, [5 different reagent kits OR 7 PPEK kits], 2 extraction control kits, 2 inhibition control kits and H1N1 kits: Assay Kits a. Encephalitis VEE Kit 1 Ref (a) b. Encephalitis WEE Kit 1 Ref (a) c. Encephalitis EEE Kit 1 Ref (a) d. Yersinia pestis Target 1 Kit 1 Ref (a) e. Yersinia pestis Target 2 Kit 1 Ref (a) f. Orthopox Kit Smallpox Target 1 1 Ref (a) g. Variola Kit Smallpox Target 2 1 Ref (a) h. Bacillus anthracis Target 1 Kit 1 Ref (a) i. Bacillus anthracis Target 2 Kit 1 Ref (a) j. Francisella tularensis Kit 1 Ref (a) k. Brucella mellitensis Kit 1 Ref (a) l. Burkholderia spp.Kit 1 Ref (a) m. Rickettsia prowazekki Kit 1 Ref (a) n. Coxiella burnetti Kit 1 Ref (a) Reagent Kits a. Platinum Path Extraction Kit (PPEK) (Part 1 of 2) 2 i. PPEK Protease (Part 2 of 2) (Refrigerated, small box) 2 Extraction/Inhibition Control Kits a. DNA Extraction Control Kits, 16 Reactions 1 Ref (a) b. RNA Extraction Control Kits, 16 Reactions 1 Ref (a) c. DNA Inhibition Control Kits, 32 Reactions 1 Ref (a) d. RNA Inhibition Control Kits, 32 Reactions 1 Ref (a)

2 Revised December 4, 2013

REMARKS: Confirm operator is JBAIDS and PPEK trained. Is Quality Assurance testing up to date?

For Official Use Only – Distribution authorized to DoD and DoD contractors only. 3