Academic Appointments

BIOGRAPHICAL SKETCH Dr VarvaresouAthanasia, POSITION TITLE Pharmacist Associate Professor in Cosmetology, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens Tel: +30 2105385640 Fax: +30 2105385861 Email: [email protected], [email protected]

Academic Appointments 1985-1989 Bachelor, School of Pharmacy, University of Athens, Greece 1990-1991 Doctoral Fellow, School of Pharmacy, University of Heidelberg, Germany 1991-1996 Ph.D. diploma, School of Pharmacy, University of Athens, Greece 1996--2002 Post-Doctoral Fellow, School of Pharmacy, University of Athens, Greece (research programs) 1996-2007 Assistant in Research and Education, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens 2007-2012 Assistant Professor in Cosmetology, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens 2012-- Associate Professor in Cosmetology, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens

Jobs 1999-2002 Hospital Pharmacist, Public Hospital Spiliopoulio, Athens, Greece 2002— Consultant in the Development of Drugs and Cosmetics, cosmetics efficacy 04/2015 tests-adverse reactions of cosmetics (principal investigator in clinical studies)

Research programs Responsible Investigator: for the research program “THALIS” with title 1. Incorporation of oligopeptides in cosmetic products-quantative evaluation of functional- skin parameters with biophysical methods. 2. Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide in cosmetic product

1 Investigator: ΕLKE: Synthesis of benzofuran derivatives (antioxidant activity) (70/4/1411, School of Pharmacy, University of Athens), PENED (Κ.Α 70/3/2742, School of Pharmacy, University of Athens), EPEAEK: Manufacture and Quality control of pharmaceutical compounds (70/3/3739, School of Pharmacy, University of Athens), and ARCHIMEDES III: Antiperspirants and Health (Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens)

Teaching Activity 1. Cosmetology I, II, III and Specific Cosmetology [Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens, (Under- graduate)], 2. Photoaging and oxidative stress: Topical application of cosmetics [Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens and Medical School of Athens, University of Athens (Post-graduate: Environment and Health]. 3. Phthalates in Cosmetics: Medical School of Athens, University of Athens (Post- graduate: Environment and Health]. 4. Adverse effects from preservatives in cosmetics: Medical School of Athens, University of Athens (Post-graduate: Environment and Health]. 5. Phthalates in cosmetics, Medical School, University of Athens of Athens (Post- graduate action: Environment and Health]. 6. Pharmaceutical chemistry laboratory lessons (School of Pharmacy, University of Athens (Under-graduate)]. 7. Pharmaceutical chemistry laboratory lessons (School of Pharmacy, University of Athens (Post-graduate)]. 8. e-class (Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens) 9. Open Academic lessons (Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens)

Αuthor: 1. Varvaresou A. «Specific Cosmetology», KAFKAS Publications, Athens 2011, ISBN: 978-960-6650-48-9 2. Tsirivas E, Varvaresou A and Papageorgiou S. «Basic Aspects in Cosmetology», PARISIANOS Publications, Athens 2013, ISBN: 978-960-394-920-6 3. Tsirivas E and Varvaresou A.«Laboratory lessons in Cosmetology I», ΤΕΙ Athens 4. Tsirivas E and Varvaresou A. «Laboratory lessons in Cosmetology III», ΤΕΙ Athens 5. Varvaresou A.«Laboratory lessons in Specific Cosmetology»,ΤΕΙ Athens

Publications in International Journals: 32 Citations (scopus): 335 IF: 26.185

2 h-index (scopus): 10

Participation in Committees for Master’s Degree: 1. Title: “Indoor air pollution.Legionella” 2. Title: “Cosmetic ingredients with hormone-mimetic activity”

Participation in Committee for PhD Degree: 1. Title: “Study and chemical analysis of substances with cosmetic activity” Department of Pharmacy and Department of Aesthetics and Cosmetology, TEI of Athens 2. Title: “Study of substances with cosmetic action” Medical School of Athens and Department of Aesthetics and Cosmetology, TEI of Athens

Reviewer: Food and Chemical Toxicology (Elsevier), Household and Personal Care (Tecnoscienze), Current Microbiology (Springer) and Process Biochemistry (Elsevier), International Journal of Cosmetic Science (Wiley)

Publications in International Journals 1. Varvaresou A., Tsotinis A., Papadaki-ValirakiA.andSiatra-PapastaikoudiTh. New Azathioxanthones: Synthesis and Cytotoxicity Biorganic and Medicinal Chemistry Letters6: 861-864 (1996) 2. Varvaresou A., Tsotinis A., Siatra-Papastaikoudi Th., Papadaki-Valiraki A., Thurston D. E., Jenkins T. C. and Kelland L. R. Novel Imidazothioxanthones: Synthesis, DNA Binding and Cytotoxicity Biorganic and Medicinal Chemistry Letters 6: 865-870 (1996). 3. Fox K. R., Thurston D. E., Varvaresou A.,Tsotinis A. and Siatra-Papastaikoudi Th.A Novel Series of DNA Triple Helix-Binding Ligands Biochemistry and Biophysical Research Communications, 224: 717-720 (1996). 4. Varvaresou A., Tsotinis A., Papadaki-Valiraki A. and Siatra-Papastaikoudi Th. Synthesis and Structure Elucidation of New Cytotoxic Azathioxanthones Journal of Heterocyclic Chemistry 33:, 917-921(1996). 5. Varvaresou A., Tsotinis A., Siatra-Papastaikoudi Th. and Calogeropoulou T. New Pyrazole-Annulated Azathioxanthenes as Potential Intercalators: Synthesis and Structure Elucidation Journal of Heterocyclic Chemistry 33: 831-835 (1996). 6. Tsotinis A., Varvaresou A., Calogeropoulou T., Siatra-Papastaikoudi Th. and Tyligada Α. Synthesis and Antimicrobial Evaluation of Indole containing Derivatives of 1,3,4-Thiadiazole, 1,2,4-Triazole and their open-chain counterparts Arzneimittel Forschung/Drug Research 47: 307-310 (1997). 7. Varvaresou A., Siatra-Papastaikoudi Th., Tsotinis A., Tsantili-KakoulidouA. and Vamvakides A. Synthesis, Lipophilicity and Biological Evaluation of Indole containing Derivatives of 1,3,4-Thiadiazole and 1,2,4-Triazole Il Farmaco53: 320-326 (1998).

3 8. Varvaresou Α., Siatra–Papastaikoudi Th., Tsantili-Kakoulidou A., Tyligada E. Synthesis and Biological Properties of some novel Indole Derivatives containing containing 1,2,4-Triazole and their corresponding thiosemicarbazides Arzneimittel Forschung/Drug Research 50: 48-54 (2000). 9. Tsantili-KakoulidouΑ., Varvaresou A., Siatra-Papastaikoudi Th.,. Raevsky O.A A Comprehensive Investigation of the Partitioning and Hydrogen Bonding Behavior of Indole Containing Derivatives of 1,3,4-Thiadiazole and 1,2,4-Triazole by Means of Experimental and Calculative Approaches Quantative Strucure Acivity Relationship 18:482-489 (1999). 10. Varvaresou Α., Iakovou K., Filippatos E., Souli C., Calogeropoulou T., Ioannidou I., Kourounakis A.. Pannecouque C., Witvrouw M., Padalko E., Neyts J., De Clercq E., Tsotinis A. Synthesis, Antiretroviral and Antioxidant Evaluation of a Series of new Benzo[b]furan Derivatives Arzneimittel Forschung/Drug Research 51:156-162 (2001). 11. Iakovou K., Varvaresou A., Kourounakis A. P., Stead K., Sugden D., TsotinisA. Design, synthesis and biological evaluation of novel conformationally restrained melatoninergic analogs Journal of Pharmacy and Pharmacology 54:147-156 (2002). 12. Varvaresou A. and Iakovou K. Derivatives of 5-Oxy-pyrido[2,3-b]quinoxaline-9- carboxylic acid: A new Tricyclic System Useful for the Synthesis of Potential Intercalators Journal of Heterocyclic Chemistry 39 : 1173-1176 (2002). 13. Varvaresou Α., Tsaoula E. and Tsirivas E. The influence of the addition of melatonin to a magnesium ascorbyl phosphate- containing formulation on the mechanical properties of human skin Review of Clinical Pharmacology and Pharmacokinetics18:177-181 (2004). 14. Varvaresou A., Iakovou K., Gikas E., Fichtner I., Fiebig H.H., Kelland L.R., Double J.A., BibbyM.C. and Hendriks H.R. Antitumor Activity of Imidazothioxanthones in Murine and Human Tumor Models In Vitro and In Vivo Anticancer Research 24: 907-920 (2004). 15. Iakovou K.,Varvaresou A., Bruni G., Massarelli P., Vavayannis A. and Kazanis M. Anti-inflammatory and Analgesic Activity οf two dicyclic analogues of Acetaminophen and its regioisomer 3-Hydroxyacetanilide Pharmaceutical Chemistry Journal 38: 239-242 (2004). 16. Varvaresou A., Tsirivas E., Tsaoula E., Protopapa E.Oxidative Stress, Photoaging and Topical antioxidant Protection Review of Cilnical Pharmacology and Pharmacokinetics18: 261-266(2004) 17. Varvaresou A., Tsirivas E., Tsaoula E., Protopapa E.Synthetic colorants commonly used in lipsticks Review of Clinical Pharmacology and Pharmacokinetics18: 11-16 (2005). 18. Varvaresou A., Tsirivas E., Tsaoula E. Isothiazolinone Biocides as Preservatives in Cosmetics Review of Cilnical Pharmacology and Pharmacokinetics 19:105-108 (2005). 19. Varvaresou A., Tsirivas E., Tsaoula E., Protopapa E. The influence of magnesium ascorbyl phosphate on moisturization of stratum corneum Review of Cilnical Pharmacology and Pharmacokinetics19: 139-142 (2005).(

4 20. Varvaresou A. Percutaneous absorption of organic sunscreens Journal of Cosmetic Dermatology 5: 53-57 (2006). 21. Varvaresou A., Tsirivas E., Iakovou K., Gikas E., Papathomas Z., Vonaparti A., Panderi I. Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams Analytica Chimica Acta 573-574: 284-290 (2006). 22. Papageorgiou S., Varvaresou A., Hatziantoniou S., Tsirivas E., Demetzos C., Papaioannou G..Photostability of retinoids: The influence of the container Review of Cilnical Pharmacology and Pharmacokinetics 21: 49-53 (2007). 23. Papageorgiou S., Varvaresou A. and Tsirivas E. The Development of Self- preserving Cosmetics Review of Cilnical Pharmacology and Pharmacokinetics22: 455-460 (2008). 24. Varvaresou A., Papageorgiou S., Tsirivas E., Protopapa E., Kintziou H., Kefala V. and Dementzos C. Self-preserving cosmetics International Journal of Cosmetic Science 31: 163-175 (2009). 25. Papageorgiou S., Varvaresou A., Tsirivas E. and Demetzos C. New alternatives to cosmetics preservation Journal of Cosmetic Science61: 107-123 (2010). 26. Varvaresou A. and Iakovou K. Molecular Modeling Study of Intercalation Complexes of Tricyclic Carboxamides with d(GCGC)2, d(CGCG)2 and d(CGCGCG)2. Journal of Molecular Modeling, 8: 2041-2050 (2011) 27. Varvaresou A. and Papageorgiou S. The development of self-preserving gels Household and Personal Care Today, 18-21 (2011) 28. Papagianni P., Varvaresou A., Papageorgiou S., Panderi I. Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals Journal of Pharmaceutical and Biomedical Analysis 56:645-649 (2011) 29. Kefala V., Kintziou H., Protopapa E., Varvaresou A., Papageorgiou S., RaikouV. Tyrosinase inhibitors from natural Sources for Potential use in the Aesthetic and Cosmetology Practice Review of Clinical Pharamacology and Pharmacocinetics (International Edition) 25: 65-68 (2011). 30. Varvaresou Α., Papageorgiou S., Protopapa E. and KatsarouA. Efficacy and tolerance study of an oligopeptide with potential anti-aging activity Cosmetics, Dermatological Sciences and Applications 1: 133-140 (2011). 31. Kefala V., Kintziou H., Protopapa E., Varvaresou A., Papageorgiou S., Raikou V. Tyrosinase inhibitors from natural Sources for Potential use in the Aesthetic and Cosmetology Practice Review of Clinical Pharamacology and Pharmacocinetics (International Edition) 25: 65-68 (2011). 32. Kalogria Ε., Varvaresou A., Papageorgiou S., Tsaknis I., Protopapa Ε, Matikas Α.and Panderi Ι. Development and validation of a pre-column derivatization HPLC procedure for the quantitation of aluminiumchlorohydrate in antiperspirant creams using quercetin as chromogenic reagent. Chromatographia 77: 1275-1281 (2014)

Participations in International Conferences:12

5 1. Varvaresou A., Tsotinis A., Siatra-Papastaikoudi Th., Papadaki-Valiraki A., Thurston D. E., Jenkins T. C. and Kelland L. R.. Synthesis of new cytotοxicimidazothioxanthones and DNA binding studies XIVth International Symposium on Medicinal Chemistry, Maastricht, The Netherlands, 8-12 September, 1996. 2. Varvaresou A., Siatra-Papastaikoudi Th., Tsantili -Kakoulidou A. Α critical appraisal of log P calculation procedures using partition coefficients and HPLC capacity factors for a series of Indole containing Derivatives of 1,3,4 -Thiadiazole and 1,2,4-Triazole 12th European Symposium on Quantitative Structure-Activity Relationships, Copenhagen, 23-28 August, 1998 3. Tsotinis A., Iakovou K., Varvaresou A., Garratt P. J., Teh M. T. and Sugden D. Melatonin Receptor Ligands: Synthesis and Biological Evaluation of New Melatonin Derivatives 6th Conference in Advanced Medicinal Chemistry, Thessaloniki, 28-30 May 1999 4. Varvaresou Α., Tsaoula E. and Tsirivas E. The influence of melatonin on the mechanical properties of human skin. A preliminary study International Conference on Oxidative stress in Skin Biology and Medicine, Andros, Greece 18-21 September 2003 5. Varvaresou A., Tsirivas E., Papathomas Z., Iakovou K., Gikas E., Panderi I. Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams Instrumental Methods of Analysis-IMA 2005, Crete, Greece, Octomber 2005. 6. Papageorgiou S., Varvaresou A. and Tsirivas E. The antimicrobial efficacy of natural ingredients for the production of self-preserving cosmetics7th Joint Meeting of AFERP,ASP,GA,PSE & SIF, Natural Products with Pharmaceutical, Nutraceutical, Cosmetic and Agrochemical Interest, August 3-8, 2008, Athens, Greece. 7. Varvaresou A., Papageorgiou S., Tsaoula E. and Tsirivas E. The toxicological safety of natural preservatives in the «Preservative free» cosmetic products XIIIthCosmoderm Joint Meeting of ESCAD and the Hellenic Society of Dermatology and Venereology, December 12-14, 2008, Athens, Greece. 8. Papageorgiou S., Varvaresou A., Tsaoula E., Tsirivas E. and Protopapa E. The efficacy of Vitamin E and Horse Chestnut, Guarana and Alfalfa extracts related to the decrease of dark circles and bags under the eyes 7th Anti-Aging Medicine World Congress, AMWC, March 19-21, 2009, Monaco. 9. Papageorgiou S., Varvaresou A., Tsaoula E. and Protopapa E. Efficacy Evaluation and Cytotoxic Profile of a Skin-Depigmentating Cosmetic Product 8th Anti-Aging Medicine World Congress, AMWC, April 8-10, 2010, Monaco. 10. Papagianni P.,Varvaresou A., Papgeorgiou S., Panderi I. Determination of oligopeptide-20 in cosmeceuticals by an isocratic ion-pair RP-HPLC method with heptafluorobutyric acid as ion-pair reagenton a cyanopropyl

6 reversed phase column The 7th International Conference on Instrumental methods of Analysis Modern Trends and Applications 18-22 September 2011, Chania, Crete. 11. Pnevmatikatos S., Kintziou H., Varvaresou A, Papageorgiou S., Protopapa E., Kefala V. Effect of sea mud compresses from Messolonghi lagoon as a treatment for cellulite [Experimental study] 9th World congress of Cosmetic Dermatology, 27-30 June 2013 Athens, Greece. 12. Kalogria Ε., Varvaresou A., Papageorgiou S., Tsaknis I., Protopapa E., Panderi I. Development and evaluation of a pre-column metal complex formation HPLC procedure for the detection and quantitation of aluminum in an antiperspirant cream 8th International Congress on Instrumental Methods of Analysis Modern Trends and Applications 15- 19 September, 2013, Thessaloniki, Greece. 13. Varvaresou A. Iakovou K. Papageorgiou S., Protopapa E. and Mellou F.Phthalates and cosmetics. Regulatory Affairs August 03-05, 2015, Florida, USA.

Participations in National Conferences: 17

Other activities 1. 2009-present: Responsible for the Laboratory of Cosmetology, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens 2. 2010-2014: Responsible for the Division of Aesthetics, Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens 3. 2014-present: Head of the Department of Aesthetics and Cosmetology, Technological Educational Institution of Athens

Research Interests  Synthesis and pharmacological study of new compounds with antitumor, anti- inflammatory, antimicrobial and antioxidant activities (Xanthenes, benzofurans, thiadiazoles, melatoninergic derivatives, pyridoquinoxalines).  Molecular modeling of DNA intercalative compounds  NMR Spectroscopy  Interaction of cosmetic dyes with DNA (xanthenes)  Preservation of cosmetics-thiadiazoles  Self-preserving cosmetics  Influence of antioxidant compounds on human skin elasticity  Development of anti-aging cosmetics  Influence of oligopeptides on human skin elasticity and wrinkles

7  Efficacy tests of cosmetics: Skin elasticity, profilometry, sebum, moisturization etc  Lipophilicity structure-activity relationships  UVA and UVB sunscreens  Skin penetration

Instrumental Experience ΝΜR Bruker AC 400 MHz, ΝΜR Bruker 200 MHz, IR Perkin -Elmer 883, UV Perkin- ElmerModelLambda 4B, HPLC WatersAssociatesModel 600E, HPLC GBCLC 1210 , HPLC Waters 1525 Cutometer SEM 474, Sebumeter SM 810, Corneometer CM 8, Μexameter, Visioscan, Skin Visiometer, Tewameter, Skin pHmeter, Determination SPF in vitro, Transdermal delivery (Franz cells)

Collaboration with Scientific Institutions and Organizations

Pharmaceutical School of Athens, University of Athens, Greece Medical school, University of Athens, Greece Institute for Organic and Pharmaceutical Chemistry, National Hellenic Foundation for Research School of Pharmacy and Biomedical Science, University of Portsmouth, England Department of Pharmacology, Medical School, University of Siena, Italy Department of Physiology and Pharmacology, University of Southampton, England Endocrinology and Reproduction Research Group, GKT School of biomedical Sciences, Kings College London Department of Computer-Aided Drug Design, Institute of Physiologically Active Compounds οf Russian Academy of Sciences, Russia Institute of Cancer Research, Sutton, England National Cancer Institute, U.S.A European Organisation for the Research and Treatment of Cancer, The Netherlands