AGES, Age, Gene/Environment Susceptibility; ARIC, Atherosclerosis Risk in Communities;

Question(s) and response For Ever-Smokers Only categories used to ascertain Question(s) and response categories used Question(s) and response categories used Study smoking history to ascertain smoking amount (packs/day) to ascertain duration (years smoked) Do you smoke cigarettes now? [Question for current smokers.] On average, how [Current & Former Smokers] About how old (Yes, No) many cigarettes do you usually smoke per day? were you when you first started smoking ( _ _ ) cigarettes? ( _ _ ) If you do not smoke cigarettes AGES now, did you ever smoke [Question for former smokers.] When you were [Former Smokers] About how old were you cigarettes regularly? (Yes, No) smoking, about how many cigarettes did you smoke when you quit smoking cigarettes? ( _ _ ) per day? ( _ _ ) Have you ever smoked On the average of the entire time you smoked, how How old were you when you first started regular cigarettes? (Yes, No) many cigarettes did you usually smoke per day? cigarette smoking? ( _ _ age) (CODE "NO" IF LESS THAN Do you now smoke cigarettes? (Yes, No) 400 CIGARETTES IN A How old were you when you stopped? LIFETIME.) ( _ _ age) ARIC During the years that you have smoked. was there ever a period of one year or more that you did not smoke cigarettes? (Yes, No) For how many years did you not smoke cigarettes? ( _ _ years) Have you ever smoked How many cigarettes a day do you usually smoke? No specific question was asked on age at starting cigarettes? (Yes/No [ascertained ( _ _ ) [ascertained at ages 23, 33, and 42 years] smoking in the B58C. at ages 23, 33 and 42 years]) Duration of regular cigarette smoking was Positive responses included therefore estimated from responses at ages 23, 33 B58C “occasional” smoking as well as and 42 years to questions on ever smoking and “regular smoking” (>=1 cigarette current smoking. per day) Duration of smoking, and therefore pack-years, were set to zero for lifelong non-smokers. CARDIA Do you still smoke cigarettes How many cigarettes do you smoke per day, on the Altogether, how many years have you smoked regularly? (Yes, No) average? ( _ _ cigarettes/day) cigarettes regularly? (Round Down).( _ _ years) [If respondent has stopped smoking cigarettes:] How many cigarettes did you smoke per day, on the average before you stopped? ( _ _ cigarettes/day) Have you smoked more than 100 On the average of the entire time you smoked, how Have you smoked cigarettes during the last 30 cigarettes or 5 packs of cigarettes many cigarettes did you smoke per day? days? (Yes, No, Don’t know) in your lifetime? (Yes, No, Don’t (_ _ cigarettes per day) How old were you when you first started to Know) CHS smoke cigarettes? ( _ _ age in years) If you have stopped smoking cigarettes completely, how old were you when you stopped? ( _ _ age in years) Have you ever smoked for as Do you now smoke, as of one month ago? How old were you when you started smoking? long as a year? (Yes, No) (Yes, No) ( _ _ years) ['YES' means at least 20 packs of [If yes,] how much do you now smoke on Do you now smoke, as of one month ago? cigarettes or 12 oz (360 grams) of average? ( _ _ number of cigarettes, cigarillos, (Yes, No) tobacco in a lifetime, or at least or cigars a week or pipe tobacco ounces/grams If NO, How old were you when you stopped one cigarette per day or one per week) ECRHS or cut down smoking? ( _ _ years) cigara week for one year] We calculated the total smoke pack per day Have you stopped or cut down smoking? assuming one smoke pack was equivalent to 20 (Yes, No) cigarettes or 6.67 cigarillo or 20 grams of pipe tobacco or 4 cigars. If Yes, How old were you when you stopped or cut down smoking? ( _ _ years)

EPIC Have you ever smoked as much [At each age 20, 30, 40, and 50], how many How old were you when you started smoking as one cigarette a day for as long cigarettes did you smoke each day? ( _ _ cigarettes regularly? ( _ _ yrs old) (obese cases as a year? (Yes, No) per day) and Do you smoke cigarettes now? (Yes, No) Do you smoke cigarettes now? [For current smokers,] how many cigarettes do you population- If you have stopped smoking, how old were you (Yes, No) smoke each day? ( _ _ ) based) when you gave up? ( _ _ yrs old) FHS Offspring cohort, examinations Original cohort, biennial examinations starting in Offspring cohort, examinations every 4-6 years every 4-6 years starting in 1972 1948 starting in 1972 Have you smoked for at least one How many cigarettes per day do you smoke? ( _ _ ) [For current and former smokers,] year? (Yes, smoke now / Yes, Offspring cohort, examinations every 4-6 years Age started smoking regularly ( _ _ ) former smoker / No) starting in 1972 If stopped, age stopped smoking ( _ _ ) Have you smoked cigarettes? Usual number of cigarettes smoked Generation 3 cohort, initial examination starting (Yes, smoke now / Yes, former ( _ _ cigarettes per day) in 2002 smoker / No) Follow-up examination Have you smoked cigarettes regularly in the last year? (Yes, No) Generation 3 cohort, initial Follow-up examination examination starting in 2002 [For current smokers,] How many cigarettes How old were you when you first started regular Have you ever smoked cigarettes do/did you smoke a day? ( _ _ ) cigarette smoking? ( _ _ ) regularly? (Yes, No) Generation 3 cohort, initial examination starting in If you have stopped smoking cigarettes [No means less than 20 pack of 2002 completely, how old were you when you cigarettes or 20 oz. of tobacco in On the average of the entire time you smoked, how stopped? ( _ _ ) a lifetime or less than 1 cigarette many cigarettes per day did you smoke? ( _ _ ) a day for a day] [If yes,] Have you smoked cigarettes regularly in the last year? (Yes, No) Have you smoked at least 100 On the average of the entire time you smoked, how How old were you when you first started cigarettes in your entire life? many cigarettes did you usually smoke per day? smoking cigarettes fairly regularly? (Yes, No, Don’t know, Refused) ( I was _ _ years old) Health ABC Do you smoke cigarettes now? (Yes, No) How old were you when you stopped smoking? ( I was _ _ years old) LifeLines Have you ever smoked for at How much have you been smoking up till now? How old were you when you started smoking? least a year? (Yes, No) From … yrs (age) till … yrs (age), I smoked … ( _ _ yrs old) Do you smoke now, or have you cigarettes/day smoked in the last month? (Yes, From … yrs (age) till … yrs (age), I smoked … No) How old were you when you stopped smoking? cigarettes/day Did you stop smoking? (Yes, No) ( _ _ yrs old) From … yrs (age) till … yrs (age), I smoked … cigarettes/day How much have you been smoking up till now? Etc. From … yrs (age) till … yrs (age), I smoked … cigarettes/day From … yrs (age) till … yrs (age), I smoked … cigarettes/day From … yrs (age) till … yrs (age), I smoked … cigarettes/day Etc. Have you ever smoked On the average of the entire time you smoked, how How old were you when you first started cigarettes? (Yes, No) many cigarettes did you smoke per day? smoking cigarettes? (age) (“No” means less than 20 packs Have you smoked cigarettes during the last 30 of cigarettes or 12 oz. of tobacco days? (Yes, No) MESA in your lifetime or less than 1 How old were you when you quit smoking cigarette a day for one year at cigarettes? any time in your life.)

Have you ever smoked in your How much per day do you usually smoke now or I started smoking when I was __ years old life? (Yes, No) smoked before you gave up something? ( _ _ cigarettes per day [filter or other], pipefuls per I have smoked regularly for altogether __ years (ascertained at the 31-year postal day, or cigars per day) (OR age at last smoke) questionnaire) (ascertained at the 31-year postal questionnaire) NFBC1966 (ascertained at the 31-year postal questionnaire) We calculated the total smoke pack per day assuming one smoke pack was equivalent to 20 filter cigarettes or 10 other cigarettes or 8 pipefuls or 5 cigars. Do you currently smoke How many cigarettes do you smoke a day? How old were you when you started smoking cigarettes? (Yes, No) ( _ _ number of cigarettes) cigarettes? ( _ _age) Did you smoke cigarettes in the How many cigarettes did you smoke a day? How old were you when you stopped smoking past? (Yes, No) ( _ _ number of cigarettes) cigarettes? ( _ _ age) For how many years did you stop smoking (Past RS-I, RS-II, smokers)? ( _ _ years) and RS-III Did you ever stop smoking cigarettes (missing for current smokers)? (Yes, No) For how many years did you stop smoking cigarettes (for current smokers)? ( _ _ years) Number of smoking years ( _ _ years) Have you ever smoked for as On average of the entire time you smoked, before How old were you when you started smoking? long as a year? (Yes, No) you stopped, how much did you smoke? ( _ _ age) ( _ _ cigarettes per day) (“Yes” means at least 20 packs of How old were you when you stopped smoking? cigarettes or 12 oz (360 grams) of How much do you now smoke on average? ( _ _ age) SAPALDIA tobacco in a lifetime, or at least ( _ _ cigarettes per day) one cigarette per day or one cigar a week for one year) Do you now smoke, as of one month ago? (Yes, No) Do you smoke cigarettes now? How many cigarettes do you now smoke a day on How old were you when you started smoking? (Yes, No) average? ( _ _ cigarettes per day) ( _ _ age) SHIP Have you ever smoked From when do you smoke this amount? (__ age) How old were you when you started smoking? cigarettes? (Yes, No) ( _ _ age) What was the highest number of cigarettes you smoked daily over a period of one year? When did you stop smoking? (__ age) Have you ever smoked a whole On the days that you smoked, how many cigarettes At what age did you start smoking? ( _ _ age) cigarette? (Yes, No) did you usually smoke? ( _ _ cigarettes per day) At what age did you start smoking daily? Have you ever smoked cigarettes During the years that you smoked daily, about how ( _ _ age) regularly (at least one cigarette many cigarette per day did you usually smoke? In your life, for how many years in total have you per day at least 30 days)? ( _ _ cigarettes per day) smoked daily? ( _ _ years) (Yes, No) How many cigarettes do you currently smoke? In total have you smoked at least ( _ _ cigarettes per day) TwinsUK 100 cigarettes in your life? (Yes, No) How long is the longest period you have sucessfully quit smoking? ( _ _ days/months/years) If you have stopped smoking, at what age did you stop altogether? ( _ _ age) On how many of the last 30 days did you smoke at least one cigarette? ( _ _ days) AGES, Age, Gene/Environment Susceptibility; ARIC, Atherosclerosis Risk in Communities; B58C, British 1958 Cohort; CARDIA, Coronary Artery Risk Development in Young Adults; CHS, Cardiovascular Health Study; ECRHS, European Community Respiratory

Health Survey; EPIC, European Prospective Investigation into Cancer and Nutrition; FEV1, forced expiratory volume in the first second; FVC, forced vital capacity; FHS, Framingham Heart Study; Health ABC, Health, Aging, and Body Composition Study; HWE, Hardy Weinberg equilibrium; MAF, minor allele frequency; MESA, Multi-Ethnic Study of Atherosclerosis; NFBC1966, Northern Finland Birth Cohort of 1966; RS, Rotterdam Study (cohorts I-III); SAPALDIA, Swiss Study on Air Pollution and Lung Diseases in Adults; SHIP, Study of Health in Pomerania; SNP, single nucleotide polymorphism.