UCSF Lab Standard Operating Procedure s7

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class: Chromium (VI) Oxide

Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Chromium(VI) oxide (also known as chromium trioxide) is a strong oxidizer that may cause a fire or explosion when mixed with combustible or organic material. It is toxic if ingested and fatal if absorbed through the skin. It may be harmful if inhaled. It causes burns by all exposure routes. It is carcinogenic to humans and is a suspected reproductive toxicant. It may cause genetic defects. Chromium(VI) oxide is mainly used for electroplating to impart a chromate film on metals making them corrosion-resistant. It is also used in the production of synthetic rubies.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 1333-82-0

Class: Oxidizer, toxic, corrosive, carcinogen, teratogen, reproductive toxin, mutagen, respiratory sensitizer

Molecular Formula: CrO3

Form (physical state): Solid

Color: Violet, red-violet

Boiling point: N/A

Chromium (VI) Oxide 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Potential Hazards/Toxicity

Chromium(VI) oxide is a strong oxidizer that may cause a fire or explosion when mixed with combustible or organic material. It is classified by IARC as Group 1, carcinogenic to humans. It is toxic if ingested and fatal in contact with skin. It may be harmful if inhaled. It causes burns by all exposure routes. Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. Causes severe skin burns and eye damage. Symptoms of exposure include allergy, asthma, difficulty breathing, skin sensitization, coughing, headache, nausea, diarrhea, and vomiting. It is a suspected human reproductive toxicant that may damage fertility or the unborn child. It may cause genetic defects. Prolonged exposure can cause serious damage to health. Chromium(VI) oxide has a permissible exposure limit (PEL) of 5 µg/m3.

Engineering Controls

Chemical fume hood. Adequate exhaust and capture filtration. Electrically grounded lines and equipment.

Personal Protective Equipment (PPE)

Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand Protection Handle with gloves. Nitrile gloves are recommended for splash protection. Fluorinated rubber gloves are recommended for immersion protection.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with chromium(VI) oxide.

Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/

Eye Protection ANSI approved, tight-fitting glasses/goggles. Face shields are recommended.

Skin and Body Protection Flame-resistant lab coat preferably made of anti-static material, long pants, closed-toe shoes.

Hygiene Measures Avoid contact with skin, eyes, and clothing. Wash hands before breaks and after handling.

First Aid Procedures

If inhaled Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Consult a physician.

Chromium (VI) Oxide 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) In case of skin contact Flush with plenty of water for at least 15 minutes while removing contaminated clothing. Take victim immediately to hospital.

In case of eye contact Flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact lenses. Consult a physician. Continue rinsing eyes during transport to hospital.

If swallowed Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.

Special Handling and Storage Requirements

Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion. Avoid dust formation. Provide adequate ventilation. Keep away from heat and other sources of ignition- No smoking.

Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated area. Hygroscopic. Heat-sensitive. Store away from combustible material. Incompatible with organic materials, phosphorus, and powdered metals.

Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct

Chromium (VI) Oxide 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP: ______(g , ml) to ______(g, ml)

Temperature range covered by this SOP: __ °C – __ °C

General Overview and Purpose: Enter the experimental purpose

Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

Any deviation from this SOP requires approval from the Principal Investigator.

Chromium (VI) Oxide 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)