Adult Cheat Sheet

Adult cheat sheet:

Hey Chaperones!!!

Just so that you feel comfortable helping us out, we wanted to give you a little info on what the students will be doing. Please feel free to direct any questions you have to us- and to feel free to walk around and ask questions of the students in your group. Your participation and interest will help them to feel interested as well!

Lecture: The first part of this will be a brief Toxicology lecture that the students will hopefully have a hand in directing. We are interested in letting the students know more about the things that they are interested in, so if you hear any good questions, make sure we don’t miss them!

Experiment: The students will first be asked to make dilutions. They have two solutions in front of them, one alcohol solution which is the “high” concentration, and one water solution which is the “control”. If the students are asked to make dilutions, they will be “medium” and “low” concentrations of the alcohol. The final solutions should be composed of:

High: this is straight from the original alcohol solution- 40ml as measured by the graduated cylinder Medium: This is 30 ml water and 10 ml from the original alcohol solution measured by the graduated cylinder. Pour in 30 ml of water and bring up to 40 ml by adding the original alcohol solution Low: this is 39 ml of water and 1 ml from the original alcohol solution measured by the graduated cylinder. Pour in 39 ml of water and bring up to 40 ml by adding the original alcohol solution using a dropper. Control: this is ONLY water. Put 40 ml (making sure to not use the same graduated cylinder as you used with the alcohol- or this will be contaminated…) of water in a graduated cylinder. This is used to show how the worms behave normally.

Each of these should be emptied into a plastic weigh boat.

The worms: The worms should originally be in a glass beaker. Once the water weigh boats have been filled with 40ml each of water, worms should be placed in these and allowed to settle into their new environment for 2-3 minutes. They will then each be placed in their treatment (high, medium, low and control) and observed at 0 minutes, 3 minutes, 6 minutes, and 10 minutes. Students should record activity of the worms (2=normal, 4=high activity, 0=no activity) and make other notes as to what they are doing. Students are allowed to use spoons and pipettes to move the worms, and to use either instrument to probe the worms to see how they respond.

Once the 10 minutes are complete, students should place the worms from each treatment back into the appropriate water weigh boat to recover.

The mystery: Students will be given a mystery to figure out. Help them to think critically about it by asking them questions. The thinking is more important than getting a “right” answer.