Optical Devices Based on Live Cell Arrays (LCA)

Electronic Supplementary Material

.(Optical devices based on Live Cell Arrays (LCA

Figure ESM 1 a) Slide-based capillary single cell LCA. (b) Bright-field image of the 20 μm) diameter PW working surface of the slide-based LCA. Magnification ×20, scale bar 0.1 mm. (c) Home-made Petri dish-based multicellular polymer LCA. (d) The SEM micrographs present the PW working surface and (e) the single PW (250μm diameter, (135μm width

.Calibration of the fluorescent signal (FI) of HSA-Mag NP .Fluorescence signals were calibrated both in NP solutions and intracellularly For calibration of NP fluorescence signal we initially used cell-free NP solutions. The emitted fluorescence from NP solutions at increasing concentrations was measured .using a spectrophotometer Increasing NP concentration resulted in a dose-dependent increase in the intensity of the emitted fluorescence (Fig. S2-a), and the plot was best fitted with a linear regression function (R2 = 0. 965). However, an exponential distribution was evident (R2=0.843) when individual cell mean FI was plotted vs the corresponding number of .(accumulated NPs (Fig S2 b

Figure ESM 2 a) FI of the HSA-Mag NP solution in DMEM medium (without phenol red) was) measured in the Victor spectrophotometer and demonstrated good approximation to

.(linear regression (R2=0.9659 b) Intracellular fluorescent signal showed exponential character of the relationship) between FI and intracellular NP number (R2=0.843). A constant change in the

.intracellular FI is dependent on the proportional change in the NP number X axis is Ln (NP number) in the ROIs calculated from particle analysis; and Y axis is intracellular FI as Gray Value Mean of each particle within the ROI (particle .(analysis

Calculation of 3D spheroid volume Volume of the A172 spheroids were calculated using Equivalent Circle Diameter (ECD) parameter, which is the diameter of a circle that has an area equal to the area of the Region Of Interest (ROI) in the images. ROIs of the spheroids grown from A172

(cells are defined in bright light images by discretionary red lines. (Figure ESM 3

.Figure ESM 3 Bright field image of multi cellular spheroid structures of glial cells formed within LCA (250 µm diameter). Red lines outline the area of the spheroid from which the

.volume is calculated. Magnification ×20, scale bar 100 μm ESM 4

Clip 1 demonstrates the transition from round cell morphology to spread one during first three hours of the monolayer formation (bright field). A172 cells with the accumulated HSA-NPs were loaded into the 250 µm LCA for monolayer formation .under real-time observation

Clip 2. The initial change in intracellular fluorescent signal was observed .simultaneously