Buchner P, Stuiver E, Westerman S, Wirtz M, Hell R, Hawkesford M, De Kok L (2004) Regulation

a CK -N b CK -K c CK -S DK546430 AM389564 BSultr1;1 actin actin actin

d CK -Fe e CK +Zn EV115770 EV049655

actin actin

Fig. S1 Marker genes expression in rapeseed seedlings under different stress conditions. a Expression of EST DK546430 homologous to AtDUR3, a high-affinity urea transporter induced by N starvation (Kojima et al. 2007) in N-deficient (-N) and control (CK) plants. b Expression of EST AM389564 homologous to AtHAK5, a potassium transporter induced by K starvation (Gierth et al. 2005) in K-deficient (-K) and control (CK) plants. c Expression of sulfate transporter BSultr1;1 induced by sulfate starvation (Buchner et al. 2004) in S-deficient (-S) and control (CK) plants. d

Expression of EST EV115770 homologous to FRO2, a ferric reductase induced by iron starvation (Robinson et al. 1999) in Fe-deficient (-Fe) and control (CK) plants. e

Expression of EST EV049655 homologous to AtHMA4, a heavy-metal P-type ATPase induced by Zn2+ toxicity (Mills et al. 2003) in Zn-toxic (+Zn) and control (CK) plants.

The materials and methods were the same as those described in the manuscript

References:

Buchner P, Stuiver E, Westerman S, Wirtz M, Hell R, Hawkesford M, De Kok L (2004) Regulation of sulfate uptake and expression of sulfate transporter genes in Brassica oleracea as affected by atmospheric H2S and pedospheric sulfate nutrition. Plant Physiol 136:3396–3408 Gierth M, Mäser P, Schroeder IJ (2005) The potassium transporter AtHAK5 functions in K+ deprivation-induced high-affinity K+ uptake and AKT1 K+ channel contribution to K+ uptake kinetics in Arabidopsis roots. Plant Physiol 137:1105- 1114 Kojima S, Bohner A, Gassert B, Yuan L, von Wirén N (2007) AtDUR3 represents the major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsis roots. Plant J 52:30–40 Mills RF, Krijger GC, Baccarini PJ, Hall JL, Williams LE (2003) Functional expression of AtHMA4, a P-1B-type ATPase of the Zn/Co/Cd/Pb subclass. Plant J 35: 164–176 Robinson NJ, Procter CM, Connolly EL, Guerinot ML (1999) A ferric-chelate reductase for iron uptake from soils. Nature 397:694–697