Pmcerulean-Lifeact-7 Sequence Number: LACT-103

pmCerulean-Lifeact-7 Sequence Number: LACT-103

ATGGTGATG - mCerulean Sequence (717 Nucleotides - 239 Amino Acids) ACCTGGGGC - mCerulean Chromophore (TWG); A206K = AAG GCCCGAACT - Lifeact Sequence (51 Nucleotides - 17 Amino Acids) LINKER - 7 Amino Acids (21 Nucleotides) between mCerulean and Lifeact ATG - Methionine Start Codon TAG TAA TGA - Stop Codons Antibiotic = Kan/Neo

Lifeact = N-terminal 17 amino acids from S. Cerevisiae Abp140

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCCACCATGGG CGTGGCCGACTTGATCAAGAAGTTCGAGTCCATCTCCAAGGAGGAGGGGGATCCACCGGTCGCCACCATGG TGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGC CACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTG CACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCG CCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCT GGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGT ACAACGCCATCAGCGACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAG ATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGA CGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGA AGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTAC AAGTAAAGCGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAA AACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGC

mCerulean-Lifeact-7 in HeLa Cells