UK NEQAS for H&I – SCHEME 6 – HLA ANTIBODY DETECTION ***Results must be submitted by the result deadline which can be found on the UK NEQAS for H&I website***

Before submitting these results, please ensure that you’re using the latest version of this form and that your laboratory has completed the Scheme 6 Methodology Questionnaire which can be downloaded from our website. The methodology questionnaire must be completed with the first submission of samples each year and after any changes to testing methodology.

Lab Number: Lab Name: (please complete) Sample identification:

Date samples received (DD/MM/YYYY): Date samples tested (DD/MM/YYYY):

IgG RESULTS FOR ASSESSMENT

Indicate if HLA IgG Class I / Class II antibodies are present for each sample and mark the detection method(s) X X E E

HLA IgG CI A HLA IgG CII A C C M M N N S S I I I I D D antibody present C antibody present C L L M M C F C F E E U (Positive/Negative/NT) (Positive/Negative/NT) U Sample ID L L

Please email the completed report by the deadline to:[email protected] NEQ 57, Issue 5 Page 1 of 4 Effective Date 15/02/17 Ref. SOP: 133/NEQ UK NEQAS for H&I – SCHEME 6 – HLA ANTIBODY DETECTION

Lab Number: Lab Name:

Sample identification:

Please complete the following IgG testing information:

Please mark the methods used to test these samples: Please indicate if the samples have been treated: CDC Luminex Single Antigen No treatment Dilution ELISA Luminex Mixed/Multi Heat Inactivation EDTA Flow cytometry Adsorb Out/SeraClean Beads Other (please specify): Other (please specify):

Does this apply to all samples?: Yes No Does this apply to all samples?: Yes No

If no, please provide further details & Sample IDs: If no, please provide further details & Sample IDs:

Please list any samples that gave ‘high background’ in the Luminex assay(s):

Please give further details (e.g. was the high background removed by Adsorb Out treatment etc.)

Comments

Please email the completed report by the deadline to:[email protected] NEQ 57, Issue 5 Page 2 of 4 Effective Date 15/02/17 Ref. SOP: 133/NEQ UK NEQAS for H&I – SCHEME 6 – HLA ANTIBODY DETECTION

Lab Number: Lab Name:

Sample identification: IgM RESULTS (Not assessed)

Indicate if HLA IgM Class I / Class II antibodies are present for each sample and mark the detection method(s) X X E E

HLA IgM CI A HLA IgM CII A C C M M N N S S I I I I D D antibody present C antibody present C L L M M C F C F E E U (Positive/Negative/NT) (Positive/Negative/NT) U Sample ID L L

Please mark the methods used to test these samples: Please indicate if the samples have been treated: CDC Luminex Single Antigen No treatment Dilution ELISA Luminex Mixed/Multi Heat Inactivation EDTA Flow cytometry Adsorb Out/SeraClean Beads Other (please specify): Other (please specify): Does this apply to all samples?: Yes No Does this apply to all samples?: Yes No If no, please provide further details & Sample IDs: If no, please provide further details & Sample IDs:

Please list any samples that gave ‘high background’ in the Luminex assay(s):

Please give further details (e.g. was the high background removed by Adsorb Out treatment etc.)

Please email the completed report by the deadline to:[email protected] NEQ 57, Issue 5 Page 3 of 4 Effective Date 15/02/17 Ref. SOP: 133/NEQ UK NEQAS for H&I – SCHEME 6 – HLA ANTIBODY DETECTION

Lab Number: Lab Name:

ADDITIONAL CDC RESULTS (Not Assessed – for participant information only)

Indicate the CDC percent panel reactive antibody (% PRA) for each sample. Enter NT if not tested.

T-Cell CDC B-Cell CDC PBL CDC

Panel Size:

%PRA %PRA %PRA Example NT 9 16

Comments

Please email the completed report by the deadline to:[email protected] NEQ 57, Issue 5 Page 4 of 4 Effective Date 15/02/17 Ref. SOP: 133/NEQ