Differentially Regulated Proteins Identified by 2D PAGE and ESI-MS/MS Analysis

Supplementary Table S1

Differentially regulated proteins identified by 2D PAGE and ESI-MS/MS analysis

Fold change: fold change expressed as the ratio of the average of normalized volumes of three

elicited replicates to that of the control. Mascot Score: Mascot MOWSE Score. Mass: observed

molecular weight; pI: observed isoelectric point; Spot: indicates the number of spot

corresponding to Figure 2 observed differentially regulated in response to different treatment

conditions.

Spot Fold Symbol Identification Accession no. Mascot Matches Sequence Mass/pI Function Homo sapiens score Coverage (%)

Differentially regulated proteins in response to Radiation 1 2 HSP70 Heat shock protein NP_006588 396 13 27 71.0/5.37 chaperone 70 2 2 TRX Thioredoxin NP_003320 118 2 25 11.8/4.82 oxidoreductase

3 4.2 PRX2 Peroxiredoxin NP_002565 53 2 5 22.0/8.27 chaperone

4 3.16 PDIA3 Protein Disulfide NP_000909 126 4 10 57.4/4.76 isomerase 7 Isomerase

5 2.28 CALR Calreticulin NP_004334 411 11 35 47.0/4.30 chaperone 7

6 4.2 YWHAZ 14-3-3 zeta NP_003397 65 2 9 27.8/4.73 adapter protein

7 2 H2B1 Histone 2B1 NP_003509 55 2 7 13.8/10.31 transcription regulation 8 2 ACTG Actin Gamma 1 NP_001605 175 7 25 42.1/5.31 cell motility

9 2 TPM4 Tropomyosin alpha- NP_003281 72 2 12 28.4/4.67 actin binding 4 chain 10 3.37 VIM Vimentin NP_003371 115 13 28 53.5/5.06 signaling (Wnt pathway) 11 2 FABPE Fatty Acid binding NP_001435 72 2 14 15.3/6.84 transporter protein, epidermal activity, PPARg signaling 12 4.2 UBCEP8 40 S ribosomal NP_002286 174 7 36 32.8/4.79 protein 0 protein modification

S1 13 3.16 NPM Nucleophosmin NP_002511 513 8 34 32.6/4.67 Binds to single 7 stranded nucleic acids 14 2.7 PCBP1 Poly rC binding NP_005007 334 8 25 38.0/6.66 Binds to single protein 1 stranded nucleic acids 15 3.37 PGM Phosphoglycerate NP_002620 39 2 8 28.9/6.67 phosphatase and mutase synthase activity 16 4.2 TPI Triosephosphate NP_001152759 100 2 9 26.8/6.51 Isomerase isomerase 17 2 ENO1 Alpha-Enolase NP_001419 60 1 4 47.3/6.99 transcriptional repressor 18 2.7 PARK7 DJ-1 protein NP_009193 50 2 8 20.0/6.33 chaperone

19 2.37 NACA Nascent NP_005585 43 2 13 23.3/4.52 transcription polypeptide coactivator associated complex alpha chain

Differentially regulated proteins in response to DMA 1 0.24 HSP70 Heat shock protein NP_006588 396 13 27 71.0/5.37 chaperone 6 70 2 0.26 TRX Thioredoxin NP_003320 118 2 25 11.8/4.82 oxidoreductase 8

3 0.39 PRX2 Peroxiredoxin NP_002565 53 2 5 22.0/8.27 chaperone 7

4 0.49 PDIA3 Protein Disulfide NP_000909 126 4 10 57.4/4.76 isomerase 8 Isomerase

5 1.98 CALR Calreticulin NP_004334 411 11 35 47.0/4.30 chaperone

7 0.26 H2B1 Histone 2B1 NP_003509 55 2 7 13.8/10.31 transcription 8 regulation

8 0.20 ACTG Actin Gamma 1 NP_001605 175 7 25 42.1/5.31 cell motility 2

9 0.22 TPM4 Tropomyosin alpha- NP_003281 72 2 12 28.4/4.67 actin binding 1 4 chain

10 0.36 VIM Vimentin NP_003371 115 13 28 53.5/5.06 signaling 1 (Wnt pathway)

11 0.31 FABPE Fatty Acid binding NP_001435 72 2 14 15.3/6.84 transporter 3 protein, epidermal activity, PPARg signaling 12 2.32 UBCEP8 40 S ribosomal NP_002286 174 7 36 32.8/4.79 protein 0 protein modification 13 0.42 NPM Nucleophosmin NP_002511 513 8 34 32.6/4.67 Binds to single stranded nucleic

S2 acids 14 0.20 PCBP1 Poly rC binding NP_005007 334 8 25 38.0/6.66 Binds to single 2 protein 1 stranded nucleic acids

Differentially regulated proteins in response to DMA+radiation

1 0.444 HSP70 Heat shock protein 70 NP_006588 396 13 27 71.0/5.37 chaperone

2 1.04 TRX Thioredoxin NP_003320 118 2 25 11.8/4.82 oxidoreductase

3 0.384 PRX2 Peroxiredoxin NP_002565 53 2 5 22.0/8.27 chaperone

4 1.06 PDIA3 Protein Disulfide NP_000909 126 4 10 57.4/4.76 isomerase Isomerase 5 0.092 CALR Calreticulin NP_004334 411 11 35 47.0/4.30 chaperone

7 0.261 H2B1 Histone 2B1 NP_003509 55 2 7 13.8/10.31 transcription regulation 8 0.439 ACTG Actin Gamma 1 NP_001605 175 7 25 42.1/5.31 cell motility

9 0.349 TPM4 Tropomyosin alpha-4 NP_003281 72 2 12 28.4/4.67 actin binding chain 10 0.444 VIM Vimentin NP_003371 115 13 28 53.5/5.06 signaling (Wnt pathway) 11 0.105 FABPE Fatty Acid binding NP_001435 72 2 14 15.3/6.84 transporter protein, epidermal activity, PPARg signaling 12 0.384 UBCEP80 40 S ribosomal protein NP_002286 174 7 36 32.8/4.79 protein modification 13 0.215 NPM Nucleophosmin NP_002511 513 8 34 32.6/4.67 Binds to single stranded nucleic acids 14 0.439 PCBP1 Poly rC binding protein NP_005007 334 8 25 38.0/6.66 Binds to single 1 stranded nucleic acids