Not Finding Nemo: Limited Reef-Scale Retention in a Coral Reef Fish

Electronic Supplementary Material

Not finding Nemo: limited reef-scale retention in a coral reef fish

Gerrit B. Nanninga  Pablo Saenz-Agudelo  Peng Zhan  Ibrahim Hoteit  Michael L. Berumen

1 ESM Table 1: Summary statistics for the 30 microsatellite loci used in parentage analysis (related to the Experimental Procedures)

Adults Locus N A Ho He F Abic01 312 14 0.564 0.671 0.160 Abic02 312 6 0.729 0.730 0.002 Abic03 312 14 0.908 0.869 -0.044 Abic04 312 29 0.889 0.906 0.020 Abic05 311 21 0.623 0.687 0.093 Abic06 312 7 0.494 0.507 0.027 Abic07 312 11 0.809 0.815 0.008 Abic08 312 27 0.901 0.912 0.012 Abic10 312 11 0.796 0.786 -0.013 Abic11 311 25 0.735 0.724 -0.015 Abic12 312 23 0.917 0.918 0.001 Abic13 311 11 0.751 0.739 -0.015 Abic14 311 31 0.827 0.866 0.044 Abic17 312 7 0.704 0.729 0.035 Abic18 312 14 0.739 0.714 -0.035 Abic19 312 8 0.771 0.777 0.008 Abic20 312 10 0.745 0.785 0.051 Abic21 312 14 0.857 0.838 -0.022 Abic22 312 16 0.914 0.884 -0.034 Abic23 312 26 0.873 0.863 -0.011 Abic24 312 11 0.646 0.633 -0.022 Abic25 310 18 0.846 0.825 -0.025 Abic26 312 8 0.650 0.633 -0.026 Abic28 312 16 0.854 0.845 -0.010 Abic29 312 6 0.637 0.634 -0.005 Abic31 311 9 0.812 0.801 -0.013 Abic32 312 8 0.726 0.718 -0.011 Abic33 312 16 0.742 0.714 -0.039 Abic34 312 11 0.656 0.653 -0.005 Abic35 312 11 0.755 0.771 0.021

2 Juveniles Locus N A Ho He F Abic01 352 15 0.608 0.706 0.139 Abic02 352 7 0.736 0.733 -0.003 Abic03 352 13 0.869 0.871 0.001 Abic04 352 31 0.909 0.910 0.001 Abic05 351 25 0.652 0.678 0.038 Abic06 352 7 0.469 0.454 -0.034 Abic07 351 12 0.803 0.817 0.017 Abic08 352 29 0.920 0.913 -0.008 Abic10 352 11 0.767 0.792 0.031 Abic11 352 20 0.716 0.717 0.002 Abic12 351 22 0.909 0.923 0.015 Abic13 351 14 0.758 0.747 -0.014 Abic14 350 30 0.837 0.874 0.043 Abic17 352 8 0.736 0.725 -0.015 Abic18 352 17 0.702 0.712 0.015 Abic19 352 9 0.778 0.765 -0.018 Abic20 352 10 0.761 0.788 0.034 Abic21 353 12 0.856 0.821 -0.042 Abic22 353 16 0.875 0.889 0.016 Abic23 352 24 0.841 0.845 0.005 Abic24 351 10 0.624 0.631 0.012 Abic25 339 18 0.785 0.806 0.027 Abic26 352 7 0.631 0.624 -0.010 Abic28 352 14 0.852 0.845 -0.009 Abic29 352 7 0.580 0.623 0.070 Abic31 348 10 0.802 0.809 0.009 Abic32 352 7 0.744 0.712 -0.046 Abic33 352 16 0.702 0.711 0.013 Abic34 352 13 0.653 0.647 -0.010 Abic35 352 10 0.730 0.747 0.023

Observations include: number of genotyped individuals (N), number of alleles (A), observed heterozygosiy (Ho), expected heterozygosity (He), and Fixation Index (F)

3 Fig S1: GPS locations of anemones at the focal reef (grey circles). Yellow circles: parent-offspring assignments, arrows indicate the direction of offspring movements.

Fig S2: Average horizontal current speeds across the vertical profile of the model domain.