Program of Events

Tuesday, November 11

2:00 - 4:20 pm Course registration: Marley Building Lobby, University of Arizona.

4:30 pm Transportation to Temple of Music and Art.

5:00 - 5:30 pm Mixer.

5:30 pm Group Dinner sponsored by Telechem: Temple of Music and Art.

7:00 - 7:45 pm Lecture 1. Introduction to the workshop and to microarrays (David Galbraith): Temple of Music and Art.

8:00 pm onwards Discussion of participants’ goals.

Wednesday, November 12

All further lectures and lab sessions will be in the Marley Building.

8:30 – 9:20 am Lecture 2. Microarray production (David Galbraith).

9:20 - 9:45 am Lecture 3. Involving statisticians in experimental design (Cheryl Vanier; David Henderson).

9:45- 10:00 am Coffee Break. Sponsored by Agilent.

10:00 – 12:30 pm Lab session 1. (a) Target preparation (RNA isolation and precipitation). (b) RNA amplification.

12:30 – 1:30 pm Lunch sponsored by Becton-Dickinson.

1:30 – 3:00 pm Lab session 2. (a) Target preparation (Quality checking by RNA gel electrophoresis, and Quantification of RNA using spectrophotometers). (b) RNA amplification (continued).

Demonstration of Bioanalyzer instrumentation (Lenore Kelly).

1:30-3:00 pm Demonstration of Microarray Printers (small groups to Marley room 814). 3:00 – 3:30 pm Coffee Break. Sponsored by PerkinElmer Life and Analytical Sciences.

3:30 ~ *6:30 pm Lab session 3. (a) Target labeling. Array cross-linking and hybridization demonstration. Demonstration of Hybridization Workstations. (b) RNA amplification -- labeling of products.

Evening Group Dinner (no host) at Gentle Ben’s.

Thursday, November 13

8:30-9:00 am Genetix desktop microarray printer demonstration (Jim Bull). Breakfast provided.

9:00 – 9:50 am Lecture 4. Issues in experimental setup and hybridization (Rangasamy Elumalai).

10:00 - 10:45 am Lecture 5. Methods for amplifying microarray signals (Fang Cheng Gong).

10:45 - 11:15 am Coffee Break.

11:15 – 1:00 pm Lab session 4. Target preparation (purification steps).

1:00 – 2:00 pm Lunch sponsored by Amersham. Presentation on Amersham’s amplification techniques.

2:00 – 2:45 pm Lecture 6. Options in profiling platforms (Michael Deyholos, University of Alberta).

2:45 ~ 5:30 pm Lab session 5. Microarray cross linking and hybridization – participants using their materials.

5:30 - 8:00 pm Group Dinner at Marriott Hotel. Sponsored by Invitrogen.

8:30 pm Lab session 5a. Put arrays to hybridize overnight. Friday, November 14

8:30 am - 12:00 pm Lab session 6. Microarray washing and scanning -- participants. Data extraction and analysis -- participants.

After scanning, and before spot finding during lab 6: Lecture 7 on spot finding/morphology/parameters issues (Cheryl Vanier).

10:15 – 10:45 am Coffee break sponsored by Invitrogen. Group Photograph.

12:00 – 1:00 pm Lunch sponsored by Biodiscovery.

1:15 - 2:15 pm. Guest lecture: Dr. Robert Tempelman (Michigan State University). “Statistical aspects of normalization and replication in microarray experiments”.

2:15 – 3:00 pm Lab session 7. Complete spot finding. Data extraction. Introduction to Genesight; upload data.

3:00 - 3:45 pm. Lecture 8. Statistical Issues I: Experimental design and data analysis. (Cheryl Vanier).

2:15 - 5:00 pm Demonstration of PerkinElmer Life and Analytical Sciences microarray scanner (small groups).

3:45 - 4:15 pm. Coffee break. Sponsored by PerkinElmer Life and Analytical Sciences.

4:15 - 4:45 pm Guest lecture: Todd Martinsky (Telechem, Inc.). “Microarray manufacturing”.

4:45 - 7:00 pm. Lab session 7: BioDiscovery: visualizing data, available normalization and transformation procedures, links to annotation, and background correction.

7:00 pm Dinner on your own.

Saturday, November 15

9:00 – 10:00 am Lecture 9. Statistical issues II: Clustering, and Bayesian statistics for microarrays (David Henderson).

10:00 - 10:30 am Lab session 8.1. Biodiscovery’s methods for differential expression detection 10:30 - 11:00 am Coffee break.

11:00 - 12:30 pm Lab session 8.2. Statistical analysis of microarray data: Dave Henderson’s software, SAS.

12:30 – 1:30 pm Lunch sponsored by Biomicrosystems.

1:30 - 4:00 pm Lab session 8.2. (continued) and 8.3. Comparison of results from statistical analyses, multiple comparison issues.

4:00 - 5:00 pm Course wrap-up (David Galbraith) and departures. Note: lab will remain open as long as is required by the participants to complete their tasks.