SUPPORTING INFORMATION

A new rhodamine based ‘turn-on’ Cu2+ ion selective chemosensor in aqueous system applicable in bioimaging

ABHISHEK MAJI, SOMENATH LOHAR, SIDDHARTHA PAL and PABITRA CHATTOPADHYAY* Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal 713

104, India

CONTENTS

S1 HRMS spectrum of L...... Figure S1 1H NMR spectrum of L...... ….………………………………...... Figure S2 FT-IR spectrum of L………………………………………...... Figure S3 Interference of different cations in presence of L and Cu2+ ions……… Figure S4 Mass spectra of L due to the addition of Cu2+ ions Figure S5 FT-IR spectrum of L in presence of Cu2+ ions ………………...... Figure S6 Emission profile of L in presence of Cu2+ ions with increase in time...... Figure S7 Linear response curve of L as a function of Cu2+ ions concentration…. Figure S8 Cytotoxic effect of L.….………………………………………………. Figure S9

S2 Figure S1. HRMS spectrum of L

S3 Figure S2. 1H NMR spectrum of L

S4 Figure S3. FT-IR spectrum of L

Figure S4. Interference of different ions (50 µM) in presence of L (20.0 µM) and Cu2+ ions (20.0 µM) in 10% ACN 20 mM HEPES buffer (pH 7.4) at 25 oC (A) Ni2+, (B) Cr3+, (C) Al3+, (D) Mg2+, (E) Zn2+, (F) Pb2+, (G) Fe2+, (H) Fe3+, (I) Cd2+, (J) Co2+, (K) Mn2+, (L) Ca2+, (M) Ba2+, (N) Na+, (O) K+ and (P) Hg2+.

S5 Figure S5. Mass spectra of L due to the addition of Cu2+ ions

Figure S6. FT-IR spectra of L in presence of Cu2+ ions

2+ Figure S7. Emission (at em = 572 nm) profile of L in presence of Cu ions (1 equivalent) with increase in time (min.)

S6 Figure S8. The linear response curve of the emission of L at 572 nm depending on Cu2+ ions concentration

Figure S9. Cytotoxic effect of L (5, 10, 25, 50 and 100 μM) in AGS cell incubated for 8 h

S7