Home Address: 16 Persimmon Lane

Name: Richard M. Niles

Home Address: 16 Persimmon Lane Huntington, WV 25701

Office Address: Dept. of Biochemistry & Molecular Biology Joan C Edwards School of Medicine Marshall University 1542 Spring Valley Drive Huntington, WV 25755-9330

Phone: (304) 696-7323 (office) (304) 696-7253 (FAX) Email: [email protected]

Date of Birth: May 8th, 1946

Marital Status: Married, two sons

Education: B.A. Biology - Rhode Island College 1968 M.S. Botany - Univ. of New Hampshire 1970 Ph.D. Plant Pathology/Biochemistry Univ. of Massachusetts - Amherst 1972

Professional Experience:

2005- Member of Board of Directors for the Marshall University Research Corporation

2004- Senior Associate Dean for Graduate Education and Research Joan C. Edwards School of Medicine Marshall University

2003- Director for Research Edwards Cancer Center Joan C. Edwards School of Medicine Marshall University Huntington, WV

1992- Professor and Chairman Dept. of Biochemistry & Molecular Biology

1 Joan C. Edwards School of Medicine Marshall University Huntington, WV

2002- Co-Director DNA MicroArray Core Facility

1997-2000 Director, Medical Sciences M.S. Program Joan C. Edwards School of Medicine Marshall University Huntington, WV

1987 - 1992 Professor of Biochemistry, Boston University School of Medicine, Boston, MA.

1985 Visiting Scientist, Dana Farber Cancer Institute Harvard University School of Medicine, Boston, MA

1980 - 1987 Associate Professor of Biochemistry, Boston University School of Medicine, Boston, MA

1975 - 1980 Assistant Professor of Biochemistry Boston University School of Medicine, Boston, MA.

1974 - 1975 Research Associate, Cell Biology, Institute for Cancer Research, Philadelphia, PA.

1972 - 1974 Postdoctoral Research Associate in Biochemistry (Dr. R.W. Butcher's Laboratory), University of Massachusetts Medical Center, Worcester, MA.

1970 - 1972 Research Assistant in Plant Pathology, University of Massachusetts, Amherst, MA.

1968 - 1970 Laboratory Instructor, Introductory Botany University of New Hampshire, Durham, NH.

Societies: American Society for Biochemistry and Molecular Biology American Society for Nutritional Sciences American Association for Cancer Research American Association for the Advancement of

2 Science American Society for Microbiology Pan American Pigment Cell Society Society for Melanoma Research

Peer Review Activity: Ad hoc reviewer for Molecular Biology Study Section, NIH, 1984-1985

Ad hoc reviewer for Cancer Therapeutics Study Section, NIH, 1987

Ad hoc reviewer for VA Merit Review Board 1987, 1988, 1996, 1997, 2000

Member, Special Review Committee - Program Project Grants, National Cancer Institute, 1988

Member, Special Review Committee, Division of Research Resources, NIH, 1989.

Member, Review Panel II, American Institute for Cancer Research (AICR), 1989-2005

Peer Reviewer for U.S. Army Breast Cancer Research Program, 1994

Member Special Review Committee, NHLBI, NIH, Aug. 1995

Appointed Chairman of Review Panel II for the AICR, 1995-2005

Member Special Review Committee for SCOR NHLBI, NIH, March 1996

Member Pathobiology Review Panel for US Army Breast Cancer Research Program, Sept. 1995, 1996, 1997, 1998

Appointed Chair of Pathobiology Review Panel for US Army Breast Cancer Research Program, 1999, 2000

Member, Special Review Panel for US Army Breast Cancer “Concept” Grant Program, May 2000

3 Ad hoc reviewer for NIH Metabolic Pathology Study Section, Nov., 2000, Feb, 2001

Permanent member NIH Metabolic Pathology Study Section, June, 2001-2003

Permanent member Oncological Sciences Merit Review Panel for the VA Research Service, 2002-2005

Chair, Training Grant Review Panel for US Army Breast Cancer Research Program, 2002

Panel Member NCI Special Emphasis Panel to review Cooperative Grants for Nutritional Modulation of Cancer, Nov. 2002

Charter Member NIH Chemo and Dietary Prevention of Cancer Study Section 2003-2004

Chair, Cell Biology I Grant Review Panel US Army Prostate Cancer Research Program, April, 2003

Panel Member NCI Special Emphasis Panel To review RFA grants on Molecular Targets of Nutrients in Prostate Cancer. Nov, 2003

Chair, Cell Biology I Grant Review Panel, US Army Prostate Cancer Research Program, April, 2005

Ad hoc member US Army Breast Cancer Programatic Review Panel, April , 2005

Chair, Cell Biology 4 Grant Review Panel US Army Breast Cancer Research Program, Aug. 2005

Reviewer for numerous publications such as J. Cell. Physiol., Cancer Res., J. Biol. Chem., Science, and Oncogene

Ad hoc member of the US Army Breast Cancer Integration Panel, Nov., 2006, 2007

4 Awards: “Researcher of the Year Award” 1999-2000 Sigma Xi – Marshall University Chapter

Alumni Achievement Award - 2001 Rhode Island College Providence, RI

Marshall University Distinguished Artists and Scholars Award, 2002-2003

Invited Talks: Control of Melanoma Metastasis Yale University School of Medicine New Haven, CT, April, 1983

Hormonal Control of the Metastatic Process Sloan-Kettering Cancer Center, NY Dec., 1984

Melanogenesis and Metastasis Rockefeller University, NY Dec., 1984

Vitamin A and Cancer University of New England College of Osteopathic Medicine Biddeford, ME, April 1986

Growth and Differentiation of Lung Epithelial Cells, American Society for Cell Biology National Meeting, 1987 (Co-Organizer)

International Breast Cancer Symposium, Shenyang, People's Republic of China, Oct., 1988.

Retinoids and Tracheal Epithelial Cells Tufts University School of Medicine Boston, MA, Oct. 1991

Retinoids and Melanoma Differentiation University of Louisville School of Medicine

5 Louisville, KY, April, 1992

Control of Melanoma Differentiation by Vitamin A, Annual AICR Symposium Washington, DC, Sept. 1992

Vitamin A and Melanoma Differentiation Marshall University School of Medicine Huntington, WV, Nov., 1992

Retinoids, Vitamins A and D in Cancer Prevention and Therapy Annual AICR Symposium Washington, DC. Sept. 1994 (Session Chair)

Retinoids and Tumor Cell Differentiation Association of Medical and Graduate Departments of Biochemistry, Jan 1996 Annual Meeting, San Juan, Puerto Rico

Vitamin A and Cancer Cell Differentiation University of Connecticut Storrs, CT., Aug. 1996

PKC and Melanogenesis CBRC - Mass. General Hospital Charlestown, MA, Oct. 1996

Retinoids and PKC in Melanoma Differentiation Dept. of Biochemistry Boston University School of Medicine Boston, MA, Oct., 1996

Control of Melanoma Growth and Differentiation by Retinoids, National Cancer Institute Bethesda, MD, Dec., 1997

Retinoids and Melanoma Differentiation Wake Forest University Comprehensive Cancer Center, Winston-Salem, NC, Feb. 1998

Regulation of Melanoma Growth and Differentiation by Retinoids Washington State University Pullman, WA., April, 1998

6 Induction of Melanoma Differentiation by Vitamina A (Retinoids) University of Osteopathic and Health Sciences Des Moines, Iowa, Nov., 1998

Control of Melanoma Growth and Differentiation by Vitamin A (Retinoids) West Virginia University Health Sciences Center, Morgantown, WV, March, 1999

Role of Protein Kinase C in the Induction of Melanoma Differentiation by Retinoids University of Louisville Louisville, KY, March, 1999

Regulation of Melanoma Growth and Differentiation by Retinoids Southern Research Institute Birmingham, AL, April, 1999

Involvement of PKC in the Regulation of Melanoma Proliferation and Differentiation by Vitamin A (Retinoids) Cornell University Ithaca, NY, May, 1999

Regulation of Melanoma Growth and Differentiation by Retinoids and Protein Kinase C Department of Biochemistry Wake Forest University School of Medicine Winston-Salem, NC, Sept. 1999

Opportunities for Biotechnology Industry in the Tri-State Area Huntington Rotary Club Huntington, WV, May 2000

Identification of Retinoid-Regulated Genes in Melanoma Cells Through the Use of DNA Arrays Experimental Biology Meeting (Workshop Talk), Orlando, FL, March, 2001

7 Retinoic Acid and Protein Kinase C: Role in Melanoma Growth and Pigmentation Avon, Inc., Corporate Research Suffern, NY, April, 2001

Identification of the Transcription Factor Tbx2 as a Vitamin A-Regulated Gene in Mouse Melanoma Cells AICR Annual Research Conference on Nutrition and Cancer Washington, D.C., July, 2001

Vitamin A (Retinoids) Regulation of Melanoma Growth and Differentiation University of Southern Maine/Maine Medical Center Research Institute Portland, ME, Oct., 2001

Vitamin A (Retinoids) Induction of Melanoma Growth Arrest and Differentiation Experimental Biology Meeting (Symposium Talk), New Orleans, LA, April, 2002

Signal Transduction Pathways Involved in Retinoid Regulation of Melanoma Growth and Differentiation University of Vermont Cancer Center Burlington, VT Dec., 2002

Vitamin A Regulation of Melanoma Growth Arrest and Differentiation Department of Nutritional Sciences Rutgers, The State University of New Jersey New Brunswick, NJ, April, 2003

Signaling Pathways Involved in Vitamin A- Induction of Growth Arrest and Differentiation in Melanoma Department of Human Nutrition The Ohio State University Columbus, OH, May, 2003

Signal Transduction Involved in Vitamin A Regulation of Melanoma Growth and Differentiation

8 Department of Biochemistry and Molecular Biology University of South Florida Jan. 2004

Signaling Pathways in Retinoid-induced Melanoma Growth Arrest and Differentiation Southern Research institute, Birmingham, AL, June 2004

Use of Gene Arrays to Identify Novel Retinoic Acid-Regulated Genes in Melanoma Cells, WVU, Morgantown, WV, Oct. 2006

Gene Expression and Epigenetics: Retinoid Regulation of Melanoma Growth and Differentiation. Department of Nutritional Sciences University of Texas – Austin Nov 2007

External Service: Appointed Member of Executive Committee for AICR Annual Conferences, 1995-present

Session Chair: Retinoids and Cell Differentiation, American Association for Cancer Research Meeting, Washington, D.C. April, 1996

Organized and Chaired Session on “Resveratrol in Cancer Prevention and Treatment” for 9th AICR Annual Research Conference, Washington, D.C., 1999

Organized and Chaired Session on “Vitamins, Antioxidants and Trace Elements in Breast and Prostate Cancer Prevention and Treatment” for 10th AICR Annual Research Conference Washington, D.C., 2000

Member of Steering Committee for the Nutrition-Gene Interaction Research Interest Section of the American Society for Nutritional Sciences, 2001-2003

Chair for Mini-Symposum “Genomics and Nutrition”

9 Experimental Biology 2001 (ASNS) Orlando, FL, April, 2001

Organized and Chaired Session on “Molecular Action of Nutrients in Cancer Prevention” for the 11th AICR Annual Research Conference, Washington, D.C., July, 2001

Chair for Mini-Symposium “Molecular Mechanism for Nutrient Prevention of Cancer” Experimental Biology 2002 (ASNS) New Orleans, LA, April, 2002

Chair for Mini-Symposium “Genomics and Nutrition” Experimental Biology 2002 (ASNS) New Orleans, LA, April, 2002

Co-Chair for Symposium “Retinoids and Cancer” Experimental Biology 2002 (ASNS) New Orleans, LA, April, 2002

Organized and Chaired Session on Vitamins and Treatment/Prevention of Cancer. AICR/WCRF International Research Conference on Food, Nutrition and Cancer, Washington, D.C. July, 2004

Organized and Chaired Session on Animal Models for the Study of Nutrition and Cancer AICR/WCFR International Research Conference on Food, Nutrition and Cancer, Washington, DC, July, 2005

University Memberships (Boston University School of Medicine) Graduate Faculty Interdepartmental Biochemistry Program Hubert H. Humphrey Cancer Center

Teaching: (Boston University School of Medicine)

1976-1977 Medical Biochemistry (24 contact hrs.) 1977-1992 Cellular Biochemistry (18 contact hrs.) 1978-1983 Biochemistry Seminar (60 contact hrs.)

10 1986-1992 Molecular Mechanisms in Growth and Development (18 contact hrs.) 1989 MMEDIC Biochemistry (medical biochemistry course for undergraduates - 16 contact hrs) 1990-1992 Course manager for Dental Biochemistry

Teaching: (Marshall University School of Medicine)

1992-present Medical Biochemistry (22 contact hrs.) 1992-present Molecular and Cell Biology (6 contact hrs.) 1993-2000 Molecular Mechanisms in Growth and Differentiation (30 contact hrs.) 1993-1997 Undergraduate Biochemistry (20 contact hrs.) 1994-present Medical Cell Biology (4 contact hrs.) 1996-present Molecular Signal Transduction (4 contact hrs.) 2000- Graduate Biochemistry (16 contact hrs.)

Graduate Students: David Krah, Ph.D., 1976-1982, practicing Opthalmalogist, Florida (Boston Univ. Snezna Rogelj, Ph.D., 1979-1984, Staff Sch. of Med.) Scientist, Los Almos National Laboratory, New Mexico Maryann Golar, Ph.D., 1981-1987, Associate Professor of Biology, Bridgewater State College, Bridgewater, MA John Stoddard, Ph.D., 1985-1989, Assistant Res. Prof, Dept of Biochemistry, Boston University School of Medicine Beth Goldstein, Ph.D., 1985-1989, (M.D. Univ, of Nebraska, 1993), Pediatric Oncology Fellow, Mount Sinai Hospital, New York Susan Rosenbaum, Ph.D., 1984-1991, Staff Scientist, USDA Nutrition Research Center, Tufts University, Boston, MA Helen Kindregen, Ph.D. , 1989-1993, Biotech patent lawyer, Boston, MA Naddem Afridi, M.D., Ph.D. , 1989-1993, Otolaryngology Fellow, Boston University Medical Center Joachim Gruber, Ph.D. , 1990-1993, 4th year medical student, Boston University School of Medicine Yonghong Xiao, Ph.D. , 1990-1994, Assistant Scientist, Immunogen, Cambridge, MA Arlene Nathanson, M.S., 1978-1981, District Sales Manager, Millipore Corp, Bedford, MA

11 Kenneth Ludwig, M.S., 1978-1982, Project Manager, Millipore Corp, Bedford, MA Susan Siegal, M.S., 1982-1987, District Sales Manager, Amersham Corp, Chicago, IL Paul O'Neil, M.S. 1983-1985, Project Manager Amicon, Lexington, MA Betsy Winslow, M.S., 1985-1988, won UpJohn travel award for outstanding student presentation at the 1988 annual meeting of the American Association for Cancer Research, Science Teacher, Portland, ME School System

Postdoctoral Fellows: Margaret Brody, Ph.D., 1980-81 current status unknown (Boston University) Rick O'Brien, M.D., 1982-84, Associate Professor of Medicine, National Jewish Hospital, Denver, CO Harold Farber, M.D., 1982-84, Associate Professor of Medicine, Boston University School of Medicine Tim Quick, Ph.D., 1990-1994, Johns Hopkins Fellow at USAID, Washington, D.C.

Graduate Students: Sejal Desai, Ph.D. , 1992-1997, Senior Scientist, Clontech, Palo Alto, CA (Marshall Univ.) awarded best Basic Science Research Marshall University Research Day, 1996 Harish Mahalingham, Ph.D. , 1992-1997, Director Product Safety and Efficacy, L’Oreal New Jersey won Young Investigator Award for the most outstanding research presentation at the 1995 annual meeting of the Pan-American Society for Pigment Cell Research Jianming Han,Ph.D. 1997-2004, Resident in Internal Medicine, Mercy Hospital, Toledo, OH Ying Huang,Ph.D. 1998-2003, Postodoctoral Fellow, Univ. of Michigan – Awarded best oral research presentation, Joan C. Edwards School of Medicine Research Day, 2003 Travel award recepient from ASBMB for presentation of research at EB-’03 in San Diego, CA Caroline Mills, 2001-2007 Sandeep Joshi 2006-present

12 Won Minority Scholar Award for presentation at American Association for Cancer Research Meeting, Washington, DC, April, 2006

Postdoctoral Fellows: Shouting Huang, Ph.D., 1992-1994, Res. Assoc. Emory University School of Medicine (Marshall Univ.) Dinakar Desai, Ph.D., 1992-1996, Assistant Res. Prof. ,Mayo Clinic, Rochester, MN Awarded a Japanese Society for the Promotion of Science Fellowship through the NIH- Fullbright program, to carry out a collaborative project between my laboratory and Dr. S. Ohno's lab at Yokohama City University School of Medicine in Japan Jian-Liang Wang, M.D. 1996-1998, Senior Res. Assoc. Univ. North Carolina, Chapel Hill, N.C. Goran Boskovic, Ph.D. 1996-2004,Manager, Marshall University Microarray Core Facility; won travel award from the ASBMB to attend and present at EB’02, New Orleans, April 2002 Jennifer Minigh, Ph.D., 2003-2005 Sarah Miles, Ph.D. 2005-present Jan Fan, Ph.D. 2006-present

Committee Assignments: (Boston Univ. School of Medicine)

University Faculty Council, 1985-1989 Advisory Panel for Facilities Planning and Management, reports to V.P. for Finance of Boston University, 1988-1992

Chairman, Institutional Biosafety Committee 1991-1992 Chairman, Blood-Borne Pathogens Task Force, 1991-1992

Medical School M.D./Ph.D. Admission Committee, 1980-1985 Scientific Advisory Committee, Hubert H. Humphrey Cancer Center, 1983-1992 Radiation Safety Committee, 1989-1992 Medical Student Advisor Program 1990- 1992 Chemical Safety Committee 1990-1992

13 Biohazard Safety Coordinating Committee 1991-1992

Graduate School Academic Policy Committee, 1980-1983 Committee on Interdisciplinary Programs, 1989-1992

Departmental Admissions Committee, 1980-1983 Appointments and Promotions Committee, 1984-1988 Parent Committee for Qualifying Examinations,1986-1988 Chairman, Student Affairs Committee, 1990- 1992

Committe Assignments (Marshall Univ. School of Medicine)

University Biotech/Science Building Steering Committee. 2004- University Research and Creative Activities Council, 2002- Institutional Biosafety Committee, 1992- 2000 Faculty Senate, 1993-1995 Patent Policy Committee, 1994-96 Presidential Commission to Study the Future of Science at Marshall University Faculty Senate Committee on Research 1995- 1999 (Chair, 1998-1999 ) Steering Committee for New Biomedical Sciences Building. Fall 2000 -2002 SubCommitte on Research for University Strategic Planning Committee, Fall, 2000

Medical School Dean's Advisory Committee, 1992-present Graduate Affairs Committee, 1992-present Search Committee for Dept. of Microbiology Chair, 1993 Director, Visiting Scholars Program, 1993- 1996 Admissions Committee, 1995-1998 Chair - Committe to establish a course in Medical Cell Biology, 1994-1995

14 Health Line for Medical Students (member of executive committee), 1998-present Task Force for Basic Science and Translational Research-Planning for Edwards Cancer Center, Fall 2000-present Research Day Committee, 2001-present SubCommittee Chair on Basic Science Departments and Graduate Education in Basic Science Department for LCME self study, 2002 Chair – committe to study revision of BMS Ph.D. program

Community Activities (Huntington, WV) Member of Our Lady of Fatima Men's Club Volunteer - Habitat for Humanity Volunteer - Huntington City Mission Member Huntington Chamber of Commerce Mentor, through the Total Village program, for Middle and early High School students interested in science

RESEARCH SUPPORT

Principal Investigator Biochemical Factors Involved in Tumor Cell Metastasis American Cancer Society - Massachusetts Division $13,004 - 12/1/75 - 11/30/76

Principal Investigator The Cyclic Nucleotide System of Type II Pneumonocytes NIH $30,000 - 6/1/76 - 5/31/78

Principal Investigator Biochemical Factors Involved in Tumor Cell Metastasis NIH $100,000 - 6/1/76 - 5/31/79

Principal Investigator The Role of Cyclic Nucleotides in Cellular Transformation and Replication American Cancer Society $37,000 - 7/1/77 - 6/30/78

Principal Investigator Cyclic AMP and Cell Replication

15 American Cancer Society $38,324 - 7/1/78 - 6/30/79

Principal Investigator Cyclic AMP Metabolism in Cultured Epithelial Cells NIH $130,567 - 5/1/79 - 4/30/82

Principal Investigator Cyclic AMP and Cell Replication American Cancer Society $47,905 - 7/1/79 - 6/30/80

Principal Investigator The Effect of Retinoids on Growth and Differentiation NIH $107,898 - 7/1/82 - 6/30/84

Principal Investigator Regulation of Melanoma Growth and Differentiation by Retinoids NIH $219,174 - 7/1/84 - 6/30/87

Principal Investigator In Vitro Studies of Airway Epithelia NIH $560,000 - 12/1/86 - 11/30/91

Principal Investigator Regulation of Tumor Cell Growth by Vitamin A American Institute for Cancer Research $109,903 - 1/1/88 - 12/31/89

Principal Investigator Regulation of Growth and Differentiation by Retinoids NIH $312,589 - 4/1/88 - 3/31/91

Principal Investigator Regulation of Tumor Cell Growth by Vitamin A American Institute for Cancer Research $110,000 - 1/1/90 - 12/31/91

Principal Investigator Regulation of Tracheal Epithelial Cell Growth and Differentiation NIH (NHLBI) $663,034 - 4/1/90 - 3/31/95

16 Principal Investigator Characterization of Retinoic Acid Receptors and Their Role in Chemoprevention of Cancer American Institute for Cancer research $110,000 - 7/1/92 - 6/30/94

Principal Investigator The Role of Protein Kinase C in Retinoid-Induced Melanoma Differentiation NIH (NCI) $329,647 - 7/1/92 - 6/30/95

Obtained Burroughs-Wellcome Visiting Professorship Award for Department of Biochemistry & Molecular Biology At Marshall University (Dr. Russell Hilf, Rochester University School of Medicine), March 1994

Principal Investigator Control of Nuclear Retinoid Receptor Expression American Institute for Cancer Research $110,000 - 1/1/95 - 12/31/97

Mentor for Dr. Elsa Mangiarua's (Dept. of Physiology, Marshall University School of Medicine) NIH-Minority Faculty Development Award, 1996-2000

Principal Investigator Retinoids and Protein Kinase C in Melanoma Differentiation NIH (NCI) $421,134 - 4/1/96 - 3/31/99

Co-Investigator (PI Dr. Gary Rankin, Chair of Pharmacology, Marshall University School of Medicine) Chemical-Induced Nephrotoxicity: Succinimides NIH $1,152,124 - 4/01/98 - 03/31/03

Principal Investigator Induction of Melanoma Differentiation by Retinoic Acid: Early Response Genes AICR $164,835 – 1/1/00 – 12/31/01

Principal Investigator Retinoids and Protein Kinase C in Melanoma Differentiation NIH $1,050,000 – 4/1/00 – 3/31/05

Obtained Burroughs-Wellcome Visiting Professorship Award for Department of Biochemistry & Molecular Biology, Marshall University to sponsor Dr. Daria Mochly-Rosen from Stanford University, Nov. 2000

17 Co-Investigator Molecular Sciences NSF-EPSCoR $3,000,000 – 1/1/02 – 12/31/04

Principal Investigator Minority Supplement to Retinoids and Protein Kinase C in Melanoma Differentiation NIH $103, 499 – 6/1/02 – 3/31/05

Principal Investigator Foundation of Basic and Translational Research for the Edwards Cancer Center West Virginia State Challenge Grant $2,398,050 – 10/1/02 – 6/30/08

Principal Investigator Resveratrol and Human Melanoma NIH $146,000 – 5/1/03 – 3/31/05

Principal Investigator Center of Biomedical Research Excellence – “Transcription Factors in Cancer” NIH, NCRR $9.3 million – 9/23/04 – 7/31/09

Principal Investigator Use of Phytochemicals to activate expression of silenced genes in melanoma cells, RO3 NIH, NCI $140,000 – 11/1/06 – 10/30/08

Publications

1. Niles, R.M. and Rich, A.E. The Effect of Various Peptone Sources on Germ Tube Elongation in Uromyces Phaseoli. Phytopathology 61: 562-564 1971.

2. Niles, R.M. and Mount, M.S. Chromatin Directed RNA synthesis: Comparison of Chromatins from Healthy, Avirulent-Inoculated, and Tumorous Vicia Faba Tissue. Plant Physiol. 52: 368-372, 1973.

3. Niles, R.M. and Mount, M.S. Failure to Detect 3',5'-Cyclic AMP in Broad Bean and Crown-Gall Tumorous Broad Bean Tissue. Plant Physiol. 54: 372-373, 1974.

18 4. Niles, R.M. and Mount, M.S. Partial Purification and Properties of Cyclic Nucleotide Phosphodiesterase from Carrot. Phytochemistry 13: 2735-2 740, 1974.

5. Chibber, B.A., Niles, R.M., Prehn, L., and Sorof, S. High Extracellular Proteolytic Activity of Tumors and Control Normal Tissues. Biochem. Biophys. Res. Comm. 65: 806-812, 1975.

6. Niles, R.M., Makarski, J.S. and Rutenburg, A.M. Inhibition of Human Prostatic Epithelial Cell Replication by Cyclic AMP and Selected Analogs. Exp. Cell Res. 102: 95-103, 1976.

7. Niles, R.M., Makarski, J.S., Kim, J.H., and Rutenburg, A.M. Adenylate Cyclase Activity in Cultured Epithelial Cells. In Vitro 13: 467-472,1977.

8. Niles, R.M., Makarski, J.S., and Benfield, J.R. Examination of Hormonally Regulated Functions in Differentially Metastasizing Melanoma Cells. Surgical Forum 28: 145-147, 1977.

9. Makarski, J.S. and Niles, R.M. Loss of Adenylate Cyclase Hormonal Sensitivity in Chemically Transformed Epithelial Cells. Exp. Cell Res.114: 191-195, 1978.

10. Niles, R.M. and Makarski, J.S. Control of Melanogenesis in Mouse Melanoma Cells of Varying Metastatic Potential. J. Natl. Cancer Inst. 61: 523-526, 1978.

11. Niles, R.M. and Makarski, J.S. Hormonal Activation of Adenylate Cyclase in Mouse Melanoma Variants. J. Cell Physiol. 96: 355-360,1978.

12. Hubbard, J.P., Williams, J.D., Niles, R.M., and Mount, M.S. The Relationship Between Glucose Repression of Endo-Polygalacturonate Trans-Eliminase and Adenosine 3',5'-Cyclic Monophosphate Levels in Erwinia Carotovora. Phytopathology 68: 95-99, 1978.

13. Niles, R.M. and Makarski, J.S. Regulation of Phosphatidylcholine Metabolism in a Model Alveolar Type 2 Cell Line. J. Biol. Chem. 254: 4324- 4326, 1979.

14. Niles, R.M., Ludwig, K., and Makarski, J.S. Differential Growth Inhibition in Two Human Carcinoma Cell Lines by Cyclic AMP Analogs. J. Natl. Cancer Inst. 63: 909-911, 1979.

15. Niles, R.M. and Logue, M.P. Isolation and Characterization of an MSH- Growth Resistant Variant of B16 Mouse Melanoma. J. Supramolec.Struc. 11: 251-258, 1980.

19 16. Smith, B.D. and Niles, R.M. Characterization of Collagen Synthesized by Normal and Chemically Transformed Rat Liver Epithelial Cell Lines. Biochemistry 19: 1820-1825, 1980.

17. Ludwig, K.W., Loewy, B., and Niles, R.M. Retinoic Acid Increases Cyclic AMP-Dependent Protein Kinase Activity in Murine Melanoma Cells. J. Biol. Chem. 255: 5999-6002, 1980.

18. Ludwig, K.W, and Niles, R.M. Suppression of Cyclic AMP-Dependent Protein Kinase Activity in Murine Melanoma Cells by 12-O- tetra- decanoylphorbol-13-acetate. Biochem. Biophys. Res.Comm. 95:296-303, 1980.

19. Niles, R.M. and Logue M.P. Isolation and Characterization of a Variant of B16 Mouse Melanoma Resistant to MSH-Induced Growth Inhibition. In Tumor Cell Surfaces and Malignancy, R.O. Hynes and C.F.Fox editors Alan R. Liss, Inc, NY., 1980.

20. Niles, R.M. and Loewy, B.P. Endogenous Inhibitor of Cyclic Nucleotide Phosphodiesterase in Cultured Human Epithelial Cells. Biochem. Biophys. Res. Comm. 99: 236-242, 1981.

21. Niles, R.M., Loewy, B., and Krah, D. Differential Sensitivity of Normal and Chemically Transformed Epithelial Cells to Cholera Toxin. Cancer Res. 41: 4075-4079, 1981

22. Niles, R.M., Loewy, B., and Krah, D. Temperature-Dependent Alteration of Cellular Morphology by Cholera Toxin in Rat Liver Epithelial Cells which are ts for Maintenance of Transformed Properties. J. Cell. Physiol. 113: 35-39, 1982.

23. Rogelj, S., Loewy, B., and Niles, R.M. The Effect of Retinoic Acid on Cyclic AMP Binding Proteins in Mouse Melanoma Cells. Eur. J. Biochem. 139: 352-359, 1984.

24. Maksvytis, H.J., Niles, R.M., Simanovsky, L., Minassian, I.A., Richardson, L.L., Hamosh, M., Hamosh, P., and Brody, J.S. In Vitro Characteristics of the Lipid-filled Intersitial Cell Associated with Postnatal Lung Growth: Evidence for Fibroblast Heterogeneity. J. Cell. Physiol. 118: 113-123, 1984.

25. Niles, R.M., Loewy, B., and Rogelj, S. The Effect of Retinoic Acid on Protein Phosphorylation in Mouse Melanoma Cells. Exp Cell Res.164: 437-448, 1986.

20 26. Niles, R.M., Christensen, T.G., Breuer, R., Stone, P.J., and Snider,G.L. Serine Proteases Stimulate Mucus Glycoprotein Release From Hamster Tracheal Ring Organ Culture. J. Lab. Clin., Med. 108: 489-497, 1986.

27. Niles, R.M. Chemical Induction of Tumor Cell Differentiation. Surv. Synth. Path. Res. 4: 282-295, 1985.

28. Niles, R.M., Wilhelm, S.A., Steele, G.D., Burke, B., Christensen, T., Dexter, D., O'Brien, M.J., Thomas, P., and Zamcheck, N. Isolation and Characterization of a Poorly/Undifferentiated Human Colon Carcinoma Cell Line (MIP-101). Cancer Invest. 5: 545-552, 1987.

29. Niles, R.M., Wilhelm, S.A., Thomas, P., and Zamcheck, N. The Effect of Sodium Butyrate and Retinoic Acid on Growth and CEA Production in a Series of Human Colorectal Tumor Cell Lines Representing Different States of Differentiation. Cancer Invest. 6: 39-45, 1988.

30. Kim, K.C., Wasano, K., Niles, R.M., Schuster, J.E., Stone, P.J., and Brody, J.S. Human Neutrophil Elastase Releases Cell Surface Mucins from Primary Cultures of Tracheal Epithelial Cells. Proc. Natl. Acad. Sci. USA 84: 9304-9308, 1987.

31. Wasano, K., Kim, K.C., Niles, R.M., and Brody, J.S. Membrane Differentiation Markers of Airway Epithelial Secretory Cells. J. Histochem.Cytochem. 36: 167-178, 1988.

32. Niles, R. Retinoic Acid-Induced Arrest of Mouse Melanoma Cells in G1 Without Inhibition of Protein Synthesis. In Vitro 23: 893-894,1987.

33. Niles, R.M., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., and Brody, J. Characterization of Extended Primary and Secondary Cultures of Hamster Tracheal Epithelial Cells. In Vitro 24: 457-462, 1988.

34. Breuer, R., Christensen, T., Niles, R., and Snider, G. Human Neutrophil Elastase Causes Glycoconjugate Release From the Epithelial Cell Surface of Hamster Trachea in Organ Culture. American Rev. Respir. Dis.139: 779-782, 1989.

35. Stoddart, J.H., Lane, M.A., and Niles, R.M. Sodium Butyrate Suppresses the Transforming Activity of an Activated N-ras Oncogene in Human Colon Carcinoma Cells. Exp. Cell Res. 184: 16-27, 1989.

36. Niles, R.M. and Loewy, B.P. Induction of Protein Kinase C in Melanoma Cells by Retinoic Acid. Cancer Res. 49: 4483-4487, 1989.

37. Golar, M.A., Goldstein, B., and Niles, R.M. Interaction Between Retinoic Acid and Cyclic AMP in the Regulation of Laminin Production

21 During F9 Teratocarcinoma Differentiation. Life Sci. Adv. 7: 181-186, 1988.

38. Goldstein, B., Rogelj, S., Siegal, S., Farmer, S.R., and Niles, R.M. Cyclic AMP-Mediated Induction of F9 Teratocarcinoma Differentiation in the Absence of Retinoic Acid. J. Cell. Physiol. 143: 205-212, 1990.

39. Niles, R.M., Loewy, B., and Brown, K. The Effect of Retinoic Acid on Growth and Proto-oncogene Expression in Hamster Tracheal Epithelial Cells. Amer. J. Respir. Cell Molec. Biol. 2: 365-371, 1990.

40. Niles, R.M., and Loewy, B. B16 Mouse Melanoma Cells Selected for Resistance to Cyclic AMP-Mediated Growth Inhibition are Cross Resistant to Retinoic Acid-Induced Growth Inhibition. J. Cell. Physiol. 147: 176-181, 1991.

41. Goldstein, B. Kindregan, H., and Niles, R.M. Effect of Growth Medium and Cyclic AMP Analogs on cAMP-Induced Differentiation of F9 Teratocarcinoma Cells. In Vitro 27: 158-162, 1991.

42. Rosenbaum, S., and Niles, R.M. Regulation of Protein Kinase C Gene Expression by Retinoic Acid in B16 Mouse Melanoma Cells. Arch. Biochem. Biophys. 294: 123-129, 1992.

43. Gruber, J., Ohno, S., and Niles, R.M. Increased Expression of PKC Plays a Key Role in Retinoic Acid-Induced Melanoma Differentiation. J. Biol. Chem. 267: 13356-13360, 1992.

44. Christensen, T.G., Breuer, R., Haddad, C.E., and Niles, R.M. Quantitative Ultrastructural Analysis of the Relationship Between Cell Growth, Shape Change, and Mucosecretory Differentiation in Cultured Hamster Tracheal Epithelial Cells Exposed to Retinoic Acid. Am J. Respir. Cell Mol. Biol. 9: 287-294, 1993.

45. Niles, R.M., Thompson, N.L., and Fenton, F. Expression of TGF- During In Vitro Differentiation of Hamster Tracheal Epithelial Cells. In Vitro : Cell. and Develop. Biol. 30A: 256-262, 1994.

46. Niles, R.M. Interactions Between Retinoic Acid and Protein Kinase C in Induction of Melanoma Differentiation. In: Diet and Cancer: Markers, Prevention and Treatment, pp 37-57 Plenum Press, NY, 1994.

47. Quick, T.C., Traish, A.M., and Niles, R.M. Characterization of Human Retinoic Acid Receptor Expressed in Recombinant Baculovirus-Infected Sf9 Insect Cells. Receptor, 4: 65-79, 1994.

22 48. Kindregan, H., Ohno, S., and Niles, R.M. Characterization of Conventional PKC Isotype Expression During F9 Teratocarcinoma Differentiation: Overexpression of PKCAlters the Expression of Some Differentiation-Dependent Genes. J. Biol. Chem. 269: 27756-27761, 1994.

49. Niles, R.M. Use of Vitamins A and D in Chemoprevention and Therapy of Cancer: Control of Nuclear Receptor Expression and Function. In: Diet and Cancer: Molecular Mechanisms of Interaction pp 1-15, Plenum Press, N.Y., 1995.

50. Desai, D., and Niles, R.M. Expression and Regulation of RXRs in B16 Mouse Melanoma Cells. J. Cell. Physiol., 165: 349-357, 1995.

51. Gruber, J.R., Desai, S., Blusztajn, J.K., and Niles, R.M. Retinoic Acid Specifically Increases Nuclear PKC and Stimulates AP-1 Transcriptional Activity in B16 Mouse Melanoma Cells. Exp. Cell Res. 221: 377-384, 1995

52. Dawson, M.I., Chao, W-R., Pine, P., Jong, L., Hobbs, P.D., Rudd, C. Quick, T.C., Niles, R.M., Zhang, X., Lombardo, A., Ely, K.R., Shroot, B., and Fontana, J.A. Correlation of Retinoid Binding Affinity to RAR with Retinoid Inhibition of Growth of Estrogen Receptor-Positive MCF-7 Mammary Carcinoma Cells. Cancer Res., 55: 4446-4451, 1995.

53. Xiao, Y., Desai, D., Quick, T.C., and Niles, R.M. Control of Retinoic Acid Receptor Expression and Function in Mouse Melanoma by Cyclic AMP. J. Cell. Physiol. 167: 413-421, 1996.

54. Niles, R.M., and Combs, R. The Relationship between Susceptibility to Retinoic Acid Treatment and Protein Kinase CExpression in Murine Melanoma Cell Lines. Exp. Cell Res. 223: 21-28, 1996.

55. Huijzer, J.C., McFarland, M., Niles, R.M., and Meadows, G.G. Phorbol 12-Myristate 13-Acetate Enhances nm23 Gene Expression in Murine Melanocytes, but not in Syngeneic B16-BL6 Melanoma Variants. J. Cell. Physiol. 166: 487-494, 1996.

56. Desai, D., Michalak, M., Singh, N.K., and Niles, R.M. Inhibition of Retinoic Acid Receptor Function and Retinoic Acid-regulated Gene Expression in Mouse Melanoma Cells by Calreticulin: A Potential Pathway for Cyclic AMP Regulation of Retinoid Action. J. Biol. Chem. 271: 15153-15159, 1996.

23 57. Niles, R.M., Mahalingam, H., Vaughn, J., Novotny, J., and Gruber, J.R. Regulation of Melanogenesis in B16 Mouse Melanoma Cells by Protein Kinase C. J. Cell. Physiol. 168: 549-558, 1996.

58. Desai, S., and Niles, R.M. Characterization of Retinoic Acid-Induced AP-1 Activity in B16 Melanoma Cells. J. Biol. Chem. 272: 12809-12815, 1997.

59. Klinge, C.M., Bodenner, D.L., Desai, D., Niles, R.M., and Traish, A.M. Binding of Type II Nuclear Receptors and Estrogen Receptor to Full and Half-Site Estrogen Response Elements in vitro. Nucleic Acids Res. 25: 1903-1912, 1997.

60. Wang, S., Desai, D., Wright, G., Niles, R.M., and Wright, G.L. Effects of Protein Kinase COverexpression on A7r5 Smooth Muscle Cell Proliferation and Differentiation. Exp. Cell Res. 236: 117-126, 1997

61. Mahalingam, H., Watanabe, A., Tachibana, M., and Niles, R.M. Characterization of Density-Dependent Regulation of the Tyrosinase Gene Promoter: Role of Protein Kinase C. Exp. Cell Res. 237: 83-92, 1997.

62. Niles, R.M. Control of Retinoid Nuclear Receptor Function and Expression. In Subcellular Biochemistry, Vol. 30: Fat-Soluble Vitamins, pp. 3-28, Quinn and Kagan eds. Plenum Press, New York, 1998.

63. Desai, D.S., Hirai, S-i., Karnes, Jr., W.E., Niles, R.M., and Ohno, S. Cloning and Characterization of the Murine PKC promoter; Identification of a Retinoic Acid Response Element. Biochem. Biophys. Res. Comm. 263: 28-34, 1999.

64. Desai,S.H., Boskovic, G., Eastham,L., Dawson,M., and Niles, R.M. Effect of Receptor-Selective Retinoids on Growth and Differentiation Pathways in Mouse Melanoma Cells. Biochem. Pharmacol. 59: 1265- 1275, 2000.

65. Niles, R.M. Recent Advances in the Use of Vitamin A (Retinoids) in the Prevention and Treatment of Cancer. Nutrition 16: 1084-1089, 2000

66. Niles, R.M. Vitamin A and Cancer. Nutrition 16: 573-576, 2000

67. Geng, W.D., Boskovic, G., Fultz, M.E., Niles, R.M., Ohno, S., and Wright, G.L. Regulation of Expression and Activity of Four PKC Isozymes in Confluent and Mechanically Stimulated UMR-108 Osteoblastic Cells. J. Cell. Physiol. 189: 216-228, 2001

24 68. Niles, R.M. The Use of Retinoids in the Prevention and Treatment of Skin Cancer. Expert Opin. Pharmacother. 3: 1-5, 2002

69. Boskovic, G., and Niles, R.M. Regulation of RAR by Protein Kinase C in Mouse Melanoma Cells., J. Biol. Chem. 277: 26113-26119, 2002

70. Huang, Y. and Niles, R.M. Inhibition of AP-1 Transcriptional Activity Attenuates Retinoic Acid-Induced Growth Arrest and Differentiation of Mouse Melanoma Cells. J Cell. Physiol. 194: 162-170, 2002

71. Niles, R.M. Vitamin A (Retinoids) Regulation of Mouse Melanoma Growth and Differentiation. J. Nutr. 133: 282-286, 2003

72. Niles, R.M., McFarland, M., Weimer, M.B., Redkar, A., Fu, Y.-M., and Meadows, G.G. Resveratrol is a Potent Inducer of Apoptosis in Human Melanoma Cells. Cancer Letters 190: 157-163, 2003

73. Boskovic, G. and Niles, RM. Identification of Tbx-2 as an Immediate Early Gene Target of Retinoic Acid in B16 Mouse Melanoma Cells. Exp. Cell Res. 295: 281-289, 2004

74. Niles, R.M. Signaling Pathways in Retinoid Chemoprevention and Treatment of Cancer. Mutat. Res. 555:81-96, 2004

75. Herdman, M., Marcelo, A., Niles, R.M., and Kiningham, K. Thimerosal Induces Apoptosis in a Neuroblastoma Model via the c-Jun N-Terminal Kinase Pathway. Toxicol Sci 92: 246-253, 2006.

76. Niles, R.M., Cook, C.P., Meadows, G.G., Fu, Y.-M., McLaughlin, J.L., and Rankin, G.O. Resveratrol is Rapidly Metabolized in Athymic (Nu/Nu) Mice and Does Not Inhibit Human Melanoma Xenograft Tumor Growth. J. Nutrition 136:2542-2546, 2006.

77. Niles, R.M. Biomarker and Animal Models for Assessment of Retinoid Efficacy in Cancer Chemoprevention. Acta Pharmacologica Sinica 28: 1383-1391, 2007.

78. Eastham, L.L., Mills, C.N., and Niles, R.M. PPAR alpha/gamma Expression and Activity in Mouse and Human Melanocytes and Melanoma Cells. Pharm. Res. Epub, 2008.

79. Huang, Y., Minigh, J., Miles, S., and Niles, R.M. Retinoic acid Decreases ATF-2 Phosphorylation and Sensitizes Melanoma Cells to Taxol- Mediated Growth Inhibition. J. Mol. Signalling Epub 2008.

25 References

Gary G. Meadows, Ph.D. Dorothy O. Kennedy Distinguished Professor Director, Cancer Prevention and Research Center Department of Pharmaceutical Sciences Box 646510, 102 Wegner Hall on Stadium Way Washington State University College of Pharmacy Pullman, WA 99164-6510. Phone (509) 335-4753 FAX (509) 335-0162 [email protected]

Frank Torti, M.D. Director, Comprehensive Cancer Center Wake Forest University Medical Center Medical Center Boulevard Winston-Salem, NC 27157-1082 (910) 716-7971 [email protected]

Howard Aulick, Ph.D. Vice-President for Research Marshall University Associate Dean for Research and Graduate Education Joan C. Edwards School of Medicine Marshall University 1542 Spring Valley Drive Huntington, WV 25704 Phone (304) 696- [email protected]

Gary O. Rankin, Ph.D. Professor and Chair Department of Pharmacoloy Marshall University School of Medicine 1542 Spring Valley Drive Huntington, WV 25755 (304) 696-7313 [email protected]

Ritva Butrum, Ph.D. Vice-President for Research American Institute for Cancer Research Washington, DC 20009 (202) 328-7744 [email protected]