Journal: Plant and Soil Article Title: Effect of Nitrogen on Uptake, Remobilization and Partitioning of Zinc and Iron throughout Wheat Development Authors: Umit Baris Kutmana, Bahar Yildiza, Ismail Cakmaka,* Affiliation: aFaculty of Engineering and Natural Sciences, Sabanci University, 34956, Istanbul, Turkey * Corresponding author E-mail: [email protected]

Online Resource 1: Three-way analysis of variance (ANOVA) of the effects of Zn supply, N supply, harvest stage and their interactions on selected traits of durum wheat (Triticum durum cv. Balcali2000) grown under greenhouse conditions

Leaf Stem Husk MSb Grain TLc Grain Tot. Grain Source of Variation DWa DW DW Yield Yield Yield Zinc Supply (A) <0.001* <0.001 <0.001 <0.001 <0.001 <0.001 Nitrogen Supply (B) <0.001 <0.001 0.331 0.011 <0.001 <0.001 Harvest Stage (C) <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 A x B <0.001 <0.001 <0.001 0.043 <0.001 <0.001 A x C <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 B x C <0.001 <0.001 0.009 <0.001 <0.001 <0.001 A x B x C <0.001 <0.001 0.024 0.004 <0.001 <0.001

Shoot Zn Shoot Fe Shoot N MS Grain TL Grain Tot. Grain Source of Variation Content Content Content Zn Conc. Zn Conc. Zn Yield Zinc Supply (A) <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 Nitrogen Supply (B) <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 Harvest Stage (C) <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 A x B <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 A x C <0.001 0.003 0.008 0.790 <0.001 <0.001 B x C <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 A x B x C <0.001 <0.001 <0.001 <0.001 0.014 <0.001

MS Grain TL Grain Tot. Grain MS Grain TL Grain Tot. Grain Source of Variation Fe Conc. Fe Conc. Fe Yield N Conc. N Conc. N Yield Zinc Supply (A) <0.001 <0.001 <0.001 <0.001 <0.001 <0.001 Nitrogen Supply (B) 0.184 0.136 <0.001 <0.001 <0.001 <0.001 Harvest Stage (C) <0.001 <0.001 <0.001 0.001 <0.001 <0.001 A x B <0.001 0.736 <0.001 <0.001 <0.001 <0.001 A x C <0.001 <0.001 <0.001 0.125 <0.001 <0.001 B x C 0.311 <0.001 <0.001 <0.001 <0.001 <0.001 A x B x C <0.001 0.331 <0.001 0.353 <0.001 <0.001

* Values are F probabilities. a Dry weight; b Main Stem; c Tiller