FIG. 1 of Supplementary Material. TLC of Chloroform/Methanol Extracts from M. Vaccae (Lanes

1 2FIG. 1 of supplementary material. TLC of chloroform/methanol extracts from M. 3vaccae (lanes 1, 2), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 47, 8) and M. parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies 5and in even lanes, extracts from rough colonies. Lane 11, GPLs of M. smegmatis 6mc2155. TLC eluted with chloroform/methanol/water (90:10:1, v/v/v) and revealed with 7anthrone. 8 9FIG. 2 of supplementary material. TLC of chloroform/methanol extracts from M. 10vaccae (lanes 1, 2), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 117, 8) and M. parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies 12and in even lanes, extracts from rough colonies. TLC eluted with chloroform/methanol 13(85:15, v/v) and revealed with anthrone. DMTs, dimycoloyl trehaloses. 14 15FIG. 3 of supplementary material. TLC of chloroform/methanol extracts from M. 16vaccae (lanes 1, 2), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 177, 8) and M. parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies 18and in even lanes, extracts from rough colonies. Lanes 11, 12 and 13, purified triacyl 19trehaloses (TATs), monomycoloyl trehaloses (MMTs) and diacyl trehaloses (DATs), 20respectively. TLC eluted with chloroform/methanol/water (30:8:1, v/v/v) and revealed 21with anthrone. 22 23FIG. 4 of supplementary material. TLC of chloroform/methanol extracts from M. 24vaccae (lanes 1, 2), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 257, 8) and M. parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies 26and in even lanes, extracts from rough colonies. TLC eluted with 27chloroform/methanol/water (60:35:8, v/v/v) and revealed with anthrone. PIMs, 28phosphatidylinositol mannosides. 29 30FIG. 5 of supplementary material. TLC of chloroform/methanol extracts from M. 31vaccae (lanes 1, 2), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 327, 8) and M. parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies 33and in even lanes, extracts from rough colonies. TLC eluted with 34chloroform/methanol/water (60:35:8, v/v/v) and revealed with molybdenum blue 35reagent. PHLs, phospholipids. 36 37FIG. 6 of supplementary material. TLC of methyl mycolates from M. vaccae (lanes 1, 382), M. gilvum (lanes 3, 4), M. obuense (lanes 5, 6), M. chubuense (lanes 7, 8) and M. 39parafortuitum (lanes 9, 10). In odd lanes, extracts from smooth colonies and in even 40lanes, extracts from rough colonies. TLC was developed three times with hexane/ether 41(85:15, v/v) and revelated with molybdophosphoric acid. I, -mycolates; II, ’- 42mycolates; IV, ketomycolates; VI, dicarboxymycolates. 43 44FIG. 7 of supplementary material. GC-MS spectra of neutral products obtained from 45saponification of chloroform/methanol extracts of M. chubuense, (A) smooth and (B) 46rough colonies. The pick eluted at 12.3 corresponds to 1-tetradecanol. 47