Ibrahim Darwish, Prof

Ibrahim Darwish, Prof. Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. Tel : +966 14677348 (Office) 0500723818 (Mobile) Fax : +966 14676220 E-mail : [email protected]

CURRICULUM VITAE

NAME Ibrahim Ali Mohamed Darwish Birth Date: Sept 05, 1966

NATIONALITY Egyptian.

CURRENT POSITION Professor in the Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Saudi Arabia.

EDUCATION 1. Ph.D. in Pharmaceutical Analytical Chemistry. “Immunoanalytical Method for Cytidine Derivatives as Cancer Markers. Setsunan University (Japan)-Assiut University (Egypt). June 1998.

2. M.Sc. in Pharmaceutical Analytical Chemistry. “ Application of 2,2-Diphenyl- 1-picryl-hydrazyl in the Analysis of Some Pharmaceutical Compounds”. Assiut University, Egypt. June 1993.

3. B.S. in Pharmaceutical Science. Very Good with Degree of Honour. Assiut University, Egypt. May 1989.

POSITIONS 1. Professor in the Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Saudi Arabia. November 2007 – date.

2. Professor in the Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Egypt. September 2008 – date. Curriculum Vitae Ibrahim Darwish, Prof.

3. Associate Professor in the Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Egypt. September 2003 – November 2007.

4. Lecturer in the same department. July 1998 – September 2003.

5. Associate Lecturer, in the same department. August 1993 – July 1998.

6. Demonstrator, in the same department. October 1989 – June 1993.

2 Curriculum Vitae Ibrahim Darwish, Prof.

FELLOWSHIPS AND AWARDS

1. Identification as Active Recognized Researcher for Publication in ISI from King Saud University, 2009.

2. Prize of Assiut University (Egypt) for the Scientific Recognition in the Filed of Pharmaceutical Research, 2009.

3. National Encouraging Prize in the Field of Biomedical Sciences from Egyptian Academy of Scientific Research and Technology, 2005.

4. Prize of Assiut University for the Best Medical Environmental Research in Assiut University, Provided by Professor Hassan Rateb, 2002.

5. Postdoctoral Fellowship from Tulane University Health Sciences Center, New Orleans, LA, USA, June 1999 – June 2001.

6. Scholarship for Studying of Ph.D. in Japan from the Ministry of High Education, Egypt, September 1995 – September 1997.

TEACHING EXPERIENCE 1. Quantitative analytical chemistry. Undergraduate students. Assiut University, Assiut, Egypt.

2. Qualitative analytical chemistry. Undergraduate students. Al-Azhar University, Assiut branch, Assiut, Egypt.

3. Volumetric analysis. Undergraduate students. King Saud University, Saudi Arabia.

4. Instrumental analysis. Undergraduate students. King Saud University, Saudi Arabia.

5. Instrumental analysis. M.Sc. students. Assiut University, Egypt.

6. Advanced pharmaceutical analysis. M.Sc. students. Assiut University, Egypt.

7. Kinetics in pharmaceutical analysis. M.Sci. students. King Saud University, Saudi Arabia.

8. Immunoanalytical technologies in pharmaceutical analysis. Ph.D. students. Assiut University, Egypt.

9. Advanced instrumental analysis. Ph.D. students. King Saud University, Saudi Arabia.

10. Advanced separation techniques. Ph.D. students. King Saud University, Saudi Arabia.

11. Advanced electrochemical methods for analysis. M.Sci. students. King Saud University, Saudi Arabia.

MAJOR RESEARCH/TECHNOLOGY TOPICS OF INTEREST

1. Development and validation of Immunoanalytical methods for pharmaceutical compounds, biomarkers and environmental pollutants.

2. Instrumental analysis by various techniques including; HPLC, LC-MS, HPLC- diodearray, capillary electrophoresis, fluorimetry, infra red spectrometry, nuclear magnetic resonance spectrometry, mass spectrometry, and spectrophotometry.

3. Biotechnology of hybridoma production for generating of monoclonal antibodies specific to small molecular weight compounds.

4. Innovations of analytical methods for therapeutically important compounds employing simple chemical reactions.

5. Quality control for analysis of drugs in dosage forms or/and in biological fluids.

SUPERVISION OF RESEARCH PROJECTS AND THESES 1. Supervision of the Initiative Bioenvironmental Research Project. Center for Bioenvironmental Research of Tulane and Xavier Universities, LA, USA, 2000.

2. M. Sci. Analysis of certain Antiviral Drugs. Faculty of Pharmacy, Assiut University, Egypt, 2006

3. M. Sci. Analytical Methods for H2-recepor Antagonists. Faculty of Pharmacy, Assiut University, Egypt, 2007.

4. M. Sci. New Spectrophotometric Methods for Analysis of Quinolones Antibiotics. Faculty of Pharmacy, Assiut University, Egypt, 2007.

5. M. Sci. Spectrophotometric and Fluorimetric Determination of Selective Serotonin Reuptake Inhibitors Using Derivatizing Reagents. College of Pharmacy (Girls), King Saud University, Saudi Arabia, 2009.

6. M. Sci. Kinetic Spectrophotometric Determination of Fluoroquinolones Containing Mon-substituted Piprazine. College of Pharmacy (Girls), King Saud University, Saudi Arabia, 2009.

7. Ph.D. A New Immunoassay Method for Quantitation of Hydroxymethylglutaryl Coenzyme A Reductase Inhibitors in Human Plasma. College of Pharmacy, King Saud University, Saudi Arabia, 2010.

8. Development of Sensitive Immunoanalytical Technique for 2'- Deoxycytidine as a Potential Prognostic Marker for Breast Cancer Chemotherapy. Research Center, College of Pharmacy, King Saud University, Saudi Arabia.

9. Synthesis and Investigation of Some Amantadine Derivatives as Hepatic Delivery System to Enhance its Activity against HCV. Research Center, College of Pharmacy, King Saud University, Saudi Arabia.

10. Immunoanalytical Study for Evaluation of 5-Methyl- 2'-Deoxycytidine as a Diagnostic Marker for Leukemia. Research Center, College of Pharmacy, King Saud University, Saudi Arabia.

11. In Vitro Investigations of Biotransformation of Some Xenobiotics by Human Intestinal Bacteria. Research Center, College of Pharmacy, King Saud University, Saudi Arabia.

12. Development of Novel Analytical Approach for Reducing the Consumption of Organic Solvents in the Charge-Transfer Based Spectrophotometric Analysis of Some Drugs Used for Treatment of Atherosclerosis. National Plan for Science and TechnologySaudi Basic Industries Corporation (SABIC) granting program, Saudi Arabia.

13. Development of Novel Immunochemistry-Based Nanosensors for Measuring of Cancer Markers in Biological Specimens. National Plan for Science and TechnologyKing Abdulaziz City for Science and Technology, Saudi Arabia.

TRAINING COURSES AND WORKSHOPS

1. Postdoctoral training in the field of biotechnology-based assessment of environment quality. Tulane University Health Sciences Center, New Orleans, LA, USA, 1999-2001.

2. Training course in OSHA regulations/exposure control of blood borne pathogens. Tulane University Medical Centre, New Orleans, LA, USA, 1999.

3. Training course in basic laboratory safety. Tulane University Medical Centre, New Orleans, LA, USA, 2000.

4. Training course in basic laboratory safety. Tulane University Medical Centre, New Orleans, LA, USA, 2000.

5. Studies for radiation protection. Tulane University Medical Centre, New Orleans, LA, USA, 2000.

6. Time management and work stresses. Faculty of Science, Assiut University, Egypt, 2005.

7. Course and curriculum design. Assiut University, Egypt, 2005.

8. Problem solving and decision making. Assiut University, Egypt, 2005.

9. Training course in Chromatography: old and new thin-layer chromatography technique. CAMAG, Riyadh, KSA, May 22, 2006.

10. Training course in merge of communication technology with teaching. King Saud University, Riyadh, Saudi Arabia, June 2008.

REVIEWING AND/OR EDITORIAL BOARDS OF SCIENTIFIC JOURNALS

1. The International Journal of Association Official of Analytical Chemists. 7 Curriculum Vitae Ibrahim Darwish, Prof.

2. International Journal of Environmental Analytical Chemistry. 3. International Journal of Biomedical Sciences. 4. Journal of Chromatographic Sciences. 5. The Canadian Journal of Analytical Sciences and Spectroscopy. 6. Spectrochimica Acta Part A: Molecular and Bimolecular Spectroscopy. 7. Environmental Monitoring and Assessment. 8. Journal of Fluorescence. 9. Food and Agricultural Chemistry. 10. Journal of Hazardous Materials. 11. Saudi Pharmaceutical Journal. 12. Saudi Chemical Society Journal. 13. Journal of King Abdul-Aziz University Medical Sciences.

MEMBERSHIP OF SOCITIES AND COMMITTES

1. American Chemical Society, 2000 to date. 2. Egyptian Syndicate of Pharmacists, July 1989 to date. 3. Egyptian Society of Pharmacists, May 1990 to date. 4. Assiut University staff members club, October 1989 to date. 5. Member of Final Exam Organization committee, College of Pharmacy, King Saud University. 6. Member of Course and Curriculum in the Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University. 7. Member of Academic Accreditation committee, College of Pharmacy, King Saud University.

LIST OF PUBLICATIONS

1. Spectrophotometric Determination of Some Pharmaceutical Compounds Using 2,2- Dipenyl-1-Picrylhydrazyl. Kamla M. Emara, Adel-Maboud I. Mohamed, H.assan F. Askal, Ibrahim A. Darwish Analytical Letters 26 (1993) 2385-2395.

2. Spectrophotometric Method for the Determination of Penicillamine and its Dosage Form. Kamla M. Emara, Ibrahim A. Darwish, Abdel-Maboud I. Mohamed, Hassan F. Askal Alexandria Journal of Pharmaceutical Sciences 7 (1993) 197-200.

3. Chiral Analysis of 3-Methoxy-4-Hydroxyphenylglycol in Human Kenji Hirose, Kenzi Asada, Ibrahim Darwish, Toshifumi Akizawa, Masanori Yoshioka Journal of Pharmaceutical and Biomedical Analysis 15 (1997) 1241-1247.

4. Preparation of a Specific Monoclonal Antibody Against 2`- Ibrahim Darwish, Toshifumi Akizawa, Kenji Hirose, Kenzi Omura, Nawal EL- Rabbat, Masanor Yoshioka Analytica Chimica Acta 365 (1998) 121-128.

5. Competitive Immunoassay Method for 5-Methyl-2`- Ibrahim Darwish , Samy Emara , Hassan Askal , Nawal El-Rabbat , Kenzi Omura , Kenji Hirose, Toshifumi Akizawa, Masanori Yoshioka Analytica Chimica Acta 413 (2000) 79-86.

6. Enzyme-Linked Immunosorbent Assay for 2`-Deoxycytidine. Ibrahim Darwish, Samy Emara, Hassan Askal, Nawal El-Rabbat, Toshifumi Akizawa, Masanori Yoshioka Analytica Chimica Acta 404 (2000) 179-186.

7. A Validated Stability-Indicating High-Performance Liquid Chromatographic Assay of Amoxicillin. Alaa Khedr, Ibrahim Darwish Bulletin of Pharmaceutical Sciences, Assiut University 23 (2000) 11-21.

8. Antibody-Based Sensors for Heavy Metal Ions. Diane A. Blake, R. Mark Jones, Robert C. Blake II, Andrey R. Pavlov, Ibrahim A. Darwish , Haini Yu Biosensors and Bioelectronics 16 (2001) 799-809.

9. One-Step Competitive Immunoassay for Cadmium Ions: Development and Validation for Environmental Water lbrahim A. Darwish, Diane A. Blake Analytical Chemistry 73 (2001) 1889-1895.

10. Development and Validation of a One-Step Immunoassay for Determination of Cadmium in Human Serum. Ibrahim A. Darwish, Diane A. Blake Analytical Chemistry 74 (2002) 52-58.

11. Spectroscopic Analytical Study for the Charge-Transfer Complexation of Certain Cephalosporins with Chloranilic Acid. Gamal A. Saleh, Hassan F. Askal, Ibrahim A. Darwish, Abdel-Nasser A. El- Shorbagi Analytical Sciences 19 (2003) 281-287.

12. Development of Generic Continuous-Flow Enzyme Immunoassay for Analysis of Aminoglycosides in Serum. Ibrahim A. Darwish

Journal of Pharmaceutical and Biomedical Analysis 30 (2003) 1539-1548.

13. On the Perspective of Capillary Electrophoresis Modes for the Determination of Morphine in Human Plasma Without Pretreatment. Samy Emara, Ibrahim Darwish, Diaa Youssef, Tsutomu Masujima. Biomedical Chromatography 18 (2004) 21-27.

14. Monoclonal Antibodies that Exhibit Allosteric Binding Behavior. R.C. Blake II , X. Li, J.B. Delehanty, I. A. Darwish, N. Ohmura, and D.A Blake Trends in Monoclonal Antibody Research. M.A. Simmons (Ed.), Nova Science Inc., Hauppauge, New York, 2005.

15. Validated Spectrophotometric and Fluorimetric Methods for Analysis of Clozapine in Tablets and Urine Ibrahim A. Darwish, Hanaa Abdel-Wadood, Niveen Abdel-Latif Annali di Chimica 95 (2005) 345-356.

16. Development and Validation of Spectrophotometric Methods for Determination of Fluoxetine, Sertraline, and Paroxetine in Pharmaceutical Dosage Forms. Ibrahim A. Darwish Journal of AOAC International 88 (2005) 38-45.

17. Simple Fluorimetric Method for Determination of Certain Antiviral Drugs via their Oxidation with Cerium (IV). Ibrahim A. Darwish, Alaa S. Khedr, Hassan F. Askal, Ramadan M. Mahmoud IL Farmaco 60 (2005) 555-562.

18. Analytical Study for the Charge Transfer Complexes of Losartan Ibrahim A. Darwish Analytica Chimica Acta 549 (2005) 212-220.

19. Kinetic Spectrophotometric Methods for Determination of Trimetazidine Dihydrochloride. Ibrahim A. Darwish Analytica Chimica Acta 551(2005) 222-231

20. Use of Oxidation Reactions for the Spectrophotometric Determination of Acyclovir and Amantadine Hydrochloride in their Dosage Forms. Ibrahim A. Darwish, Alaa S. Khedr, Hassan F. Askal, Ramadan M. Mahmoud Analytical Chemistry: an Indian Journal 1 (2005).

21. Spectrophotometric Analysis of Selective Serotonin Reuptake Inhibitors Based on Formation of Charge-Transfer Complexes with Tetracyanoquinondimethane and Chloranilic Acid. Ibrahim A. Darwish, Ibrahim H. Refaat Journal of AOAC International 89 (2006) 362-333.

22. Generic Non-Extractive Spectrophotometric Method for Determination of 4-Quinolone Antibiotics via Formation of Ion-pair

IbrahimComplexes A. Darwish, with -Naphthol. Ibrahim H. Refaat, Hassan F. Askal, Mostafa A. El-Moez Journal of AOAC International 89 (2006) 334-340.

23. Application of Inorganic Oxidants to the Spectrophotometric Determination of Ribavirin in Bulk and Capsules. Ibrahim A. Darwish, Alaa S. Khedr, Hassan F. Askal, Ramadan M. Mahmoud Journal of AOAC International 89 (2006) 341-351.

24. Simple and Sensitive Spectrophotometric Methods for Determination of Amantadine Hydrochloride. Ibrahim A. Darwish, Alaa S. Khedr, Hassan F. Askal, Ramadan M. Mahmoud Journal of Applied Spectroscopy 37 (2006) 706-711.

25. Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Ibrahim A. Darwish International Journal of Biomedical Sciences 2 (2006) 217-235.

26. Simple Generic Enzyme Immunoassay Approach to Avert Small Molecule Immobilization Problems on Solid Phases: Application to the Determination of Tobramycin in Serum.

Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdel-Rahman A. Majed, Talanta 72 (2007) 1322-1328.

27. Evaluation of N-Bromosuccinimide as a New Analytical Reagent for the Spectrophotometric Determination of Fluoroquinolone Antibiotics

Hassan F. Askal, Ibrahim H. Refaat, Ibrahim A. Darwish, Mostafa A. Marzouq Chemical & Pharmaceutical Bullutin (Tokyo), 55 (2007) 1551-1556.

28. Sensitive Determination of Trimetazidine Dihydrochloride dihydrochloride in Spiked Human Plasma by HPLC with Fluorescence Detection after Pre-column Derivatization with 9-Fluorenylmethyl chloroformate.

Alaa S. Khedr, Mahmoud M. Sheha, Ibrahim A. Darwish Journal of Chromatography B Biomedical Applications 856 (2007) 337-342.

29. Sensitive Indirect Spectrophotometric Method for

Determination of H2-Receptor Antagonists in Pharmaceutical Formulations

Ibrahim A. Darwish, Samiha A. Abdel-Rahman, Ashraf M. Mahmoud, Ahmed I. Hassan International Journal of Biomedical sciences, 3 (2007) 124-131.

30. A Sensitive Spectrophotometric Determination of H2-Receptor Antagonists Using N-Bromosuccinimide and P- Aminophenol

Ibrahim A. Darwish, Samiha A. Abdel-Rahman, Ashraf M. Mahmoud, Ahmed I. Hassan Acta Pharmaceutica 58 (2008) 87-97.

31. Spectrophotometric Determination of H2-Receptor Antagonists via their Oxidation with Cerium (IV)

Ibrahim A. Darwish, Samiha A. Abdel-Rahman, Ashraf M. Mahmoud, Ahmed I. Hassan Spectrochimica Acta A 69 (2008) 33-40.

32. Stability-Indicating Thin-Layer Chromatographic Method for Quantitative Determination of Ribavirin.

Ibrahim A. Darwish, Alaa S. Khedr, Hassan F. Askal, Ramadan M. Mahmoud Journal Chromatographic Sciences 46 (2008) 4-9.

33. A Simple Quantitative Thin-Layer Chromatographic Method for Determination of Amantadine Hydrochloride.

Hassan F. Askal, Ibrahim A. Darwish, Alaa S. Khedr, Ramadan M. Mahmoud International Journal of Biomedical Sciences 4 (2008) 155-160.

34.Nonextractive Procedure and Precolumn Derivatization with 7- Chloro-4-nitrobenzo-2-oxa-1,3-diazole for Trace Determination of Trimetazidine in Plasma by HPLC with Fluorescence Detection Ibrahim A. Darwish, Ashraf M. Mahmoud, Nasr Y. Khalil

Journal of AOAC International 91 (2008) 1037-1044.

35.New Spectrofluorimetric Method with Enhanced Sensitivity for Determination of Paroxetine in Dosage Forms and Plasma Ibrahim A. Darwish, Sawsan M. Amer, Heba H. Abdine, Lama I. Al-Rayes

Analytical Chemistry Insights 3 (2008) 145-155.

36.A Highly Sensitive Enzyme Immunoassay for Evaluation of 2'- Deoxycytidine Plasma Level as a Prognostic Marker for Breast Cancer Chemotherapy Ibrahim A. Darwish, Ashraf M. Mahmoud, Tarek Aboul-Fadl, Abdul-Rahman A. Al- Majed, Nasr Y. Khalil

Analytica Chimica Acta 632 (2009) 266-271.

37.Spectrophotometric Study for the Reaction of Fluvoxamine 1,2- naphthoquinone-4-sulphonate: Kinetic, Mechanism, and Use for Determination of Fluvoxamine in its Dosage Forms Ibrahim A. Darwish, Heba H. Abdine, Sawsan M. Amer, Lama I Al-Rayes

Spectrochimica Acta A 72 (2009) 897-902.

38.Novel Selective Kinetic Spectrophotometric Method for Determination of Norfloxacin in its Pharmaceutical Formulations Ibrahim A. Darwish, Maha A. Sultan, Hessa A. Al-Arfaj

Talanta 78 (2009) 1383-1388.

39.Spectrofluorimetric Determination of Fluvoxamine in Dosage Forms and Plasma Derivatization with 4-Chloro-7-nitrobenzo-2- oxa-1,3-diazole Ibrahim A. Darwish, Sawsan M. Amer, Heba H. Abdine, Lama I Al-Rayes

Journal of Fluorescence 19 (2009) 463-471.

40.New non-extractive HPLC Method for Determination of Paroxetine in plasma after Precolumn Derivatization with 7- Chloro-4-nitrobenzo-2-oxa-1,3-diazole Ibrahim A. Darwish, Abdul-Rahman A. Al-Majed, Ashraf M. Mahmoud, Nasr Y. Khalil

Journal of Association Official of Analytical Chemists 92 (2009) 1349-1355.

41.Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4- sulphonate Ashraf M. Mahmoud, Nasr Y. Khalil, Ibrahim A. Darwish, Tarek Aboul-Fadl

International Journal of Analytical Chemistry 2009 (2009) 1-8. doi:10.1155/2009/810104

42.New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations Ibrahim A. Darwish, Heba H. Abdine, Sawsan M. Amer, Lama I. Al-Rayes

International Journal of Analytical Chemistry 2009 (2009) ID 257309, 9 Pages.

43.Simple Spectrophotometric Method for the Determination of Paroxetine in Tablets Using 1,2-naphthoquinone-4-sulphonate as a chromogenic Reagent

Ibrahim A. Darwish, Heba H. Abdine, Sawsan M. Amer, Lama I. Al-Rayes

International Journal of Analytical Chemistry 2009 (2009) ID 237601, 8 Pages.

44.New Highly sensitive Enzyme Immunoassay for Determination of Pravastatin in Human Plasma. Ibrahim A. Darwish, Abdul-Rahman M. Al-Obaid, Hamoud A. Al-Malaq

Talanta 79 (2009) 1478-1483.

45.Novel Enzyme-Linked Immunosorbent assay for Determination of Fluvastatin in Plasma at picogram Level. Ibrahim A. Darwish, Abdul-Rahman M. Al-Obaid, Hamoud A. Al-Malaq

Talanta 80 (2009) 179-183.

46. Preparation of A Highly Specific Polyclonal Antibody Against Fluvastatin and its Use In Development of ELISA For Determination of Fluvastatin in Plasma Ibrahim A. Darwish, Abdul-Rahman M. Al-Obaid, Hamoud A. Al-Malaq

Analytical Methods 1 (2009) 220-224.

47. Spectral Investigation and Analytical Applications of the Vinylamino-substituted Haloquinone Derivatives of Nizatidine and Ranitidine. Ibrahim A. Darwish, Samiha A. Hussein, Ashraf M. Mahmoud, Ahmed I. Hassan Int. J. Res. Pharm. Sci. (IJRPS) 1 (2010) 6-12.

48.Kinetic Spectrophotometric Method for Determination of Ciprofloxacin and Lomefloxacin in their Pharmaceutical Formulations Ibrahim A. Darwish, Maha A. Sultan, Hessa A. Al-Arfaj

Int. J. Res. Pharm. Sci. (IJRPS) 1 (2010) 43-50.

49.Selective Kinetic Spectrophotometric Method for Determination of Gatifloxacin Based on Formation of its N- Vinylchlorobenzoquinone derivative Ibrahim A. Darwish, Maha A. Sultan, Hessa A. Al-Arfaj

Spectrochim. Acta A 75 (2010) 334-339.

50. Fluorometric Study for the Reaction Between Sertraline and 7- chloro-4-nitrobenzo-2-oxa-1,3-diazole: Kinetics, Mechanism and Application for the Determination of Sertraline in Tablets. Ashraf M. Mahmoud, Ibrahim A. Darwish, Nasr Y. Hkalil. Journal of Fluorescence 20 (2010) 607-613.

51.New Sensitive HPLC Method for Evaluation of the Pharmacokinetics of New Amantadine Prodrugs as Hepatic Delivery Systems to Enhance its Activity against HCV. Ibrahim A. Darwish, Tarek Aboul-Fadl, Nasr Y. Khalil, Ashraf M. Mahmoud, Abdul- Rahman M. Al-Obaid Int. J. Res. Pharm. Sci. (IJRPS) 2 (2010) 151-157.

52.Highly Sensitive HPLC Method with Automated Co-Sense System and Fluorescence Detection for Determination of Sertraline in Human Plasma. Nasr Y. Khalil, Ashraf M. Mahmoud, Ibrahim A. Darwish, Abdul-Rahman A. Al-Majed Chromatographia 71 (2010) 825-831.

53.N-Bromosuccinimide/Fluorescein System for Spectrophotometric

Determination of H2-Receptor Antagonists In Their Dosage Forms.

Ibrahim A. Darwish, Samiha A. Abdel-Rahman, Ashraf M. Mahmoud, Ahmed I. Hassan Int. J. Res. Pharm. Sci. (IJRPS) 2 (2010) 86-93.

MANUSCRIPTS UNDER PUBLICATION

1. A Highly Sensitive and Specific Polyclonal Antibody-Based Enzyme Imunoassay for Therapeutic Monitoring and Pharmacokinetic Studies of Atorvastatin.

Ibrahim A. Darwish, Hamoud A. Al-Malaq, Abdul-Rahman M. Al-Majed Microchim. Acta (2010) In Press.

2. Novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis of losartan potassium.

Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdul-Rahman A. Al-Majed Int. J. Res. Pharm. Sci. (IJRPS), Submitted on Feb. 3, 2010.

3. Development and Validation of Enzyme-Linked Imunosorbent Assay for Determination of Rosuvastatin.

Ibrahim A. Darwish, Hamoud A. Al-Malaq, Abdul-Rahman M. Al-Majed Anal. Lett., Submitted on Feb. 15, 2010.

4. Generation of a Specific Polyclonal Antibody with High Affinity to Atorvastatin and its Employment in the Development of ELISA for Determination of Atorvastatin in Plasma.

Ibrahim A. Darwish, Hamoud A. Al-Malaq, Abdul-Rahman M. Al-Majed Anal. Sci., Submitted on March 8, 2010.

5. New highly selective reaction for the spectrophotometric determination of lomefloxacin in its pharmaceutical dosage forms.

Hassan F. Aska, Ibrahim H. Refaat, Ibrahim A. Darwish, Mostafa A. Marzouq Spectrochim. Acta, Submitted on March 8, 2010.

CONTRIBUTIONS IN BOOKS

1. Monoclonal Antibodies that Exhibit Allosteric Binding Behavior. R.C. Blake II , X. Li, J.B. Delehanty, I. A. Darwish, N. Ohmura, and D.A Blake Trends in Monoclonal Antibody Research. M.A. Simmons (Ed.), Nova Science Inc., Hauppauge, New York, 2005, pp. 1-36.

2. Antibodies to Heavy metals: Isolation, Characterization, and Incorporation into Microplate Based assays and immunosensors, In: Immunoassay and other Bioanalytical Techniques. D.A Blake, R.C. Blake II , Elizabeth R. Abboud, Xia Li, Haini Yu, Alison M. Kriegel, Mehraban Khosraviani, Ibrahim A. Darwish. J.M. Van Emon (Ed), CRC Press, Taylor & Francis Group, New York, 2007, pp. 93-111.

3. Qualitative Analytical Chemistry for Pharmacy Students. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish .رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. 2005 / 1614

4. Laboratory Manual for Qualitative Analytical Chemistry. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. .2005 /1613

5. Quantitative Analytical Chemistry: Volumetric and Gravimetric Analysis. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish .رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. 2005 / 1615

6. Practical Quantitative Analytical Chemistry: Volumetric and Gravimetric Analysis. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish .رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. 2005 / 1616

7. Quantitative Analytical Chemistry: Instrumental and Applied Analysis. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish

.رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. 2005 / 1617

8. Practical Quantitative Analytical Chemistry: Instrumental and Applied Analysis. Gamal Saleh, Hassan Askal, Ibrahim Refaat, Ibrahim Darwish .رقم اليداع بدار الكتب المصرية : Marwat Alkhair Publishing, Assiut, 2005. 2005 / 1618

CONFERENCES AND PRESENTATIONS

1. The 7th International Symposium on Pharmaceutical and Biomedical Analysis. Osaka, Japan, August, 1996. “Chiral Analysis of 3-Methoxy-4-hydroxyphenylglycol in Human Serum”. Kenji Hirose, Kenzi Asada, Ibrahim Darwish, Toshifumi Akizawa, Masanori Yoshioka.

2. The 36th Society of Japanese Journal of Clinical Chemistry. Yamanashi, Japan, October 24-25, 1996. “Preparation of Monoclonal Antibody to 5-Methyl-2`-deoxycytidine”. Ibrahim Darwish, Kenzi Omura, Kenji Hirose, Toshifumi Akizawa, Masanori Yoshioka.

3. The International Cyber Congress on Analytical Biosciences. Hiroshima, Japan, August 21-22, 1997. “Preparation of Highly Specific Monoclonal Antibody against 2`- Deoxycytidine”. Ibrahim Darwish, Toshifumi Akizawa, Kenji Hirose, Masanori Yoshioka.

4. The 4th International Symposium on Impact of Cancer Biotechnology on Diagnostic and Prognostic Indicators in Predictive Oncology and Therapy. Nice, France, October 24-27, 1998. “ Development of Enzyme Immunoassay of 2`-Deoxycytidine as a Prognostic Marker”. Masanori Yoshioka, Ibrahim Darwish, Kenji Hirose, Nawal El-Rabbat.

5. The 2nd Symposium on Environmental Hormones: Past, Present, and Future. New Orleans, LA, USA, October 18-20, 1999.

6. The 52nd Southwest/Southeast Regional American Chemical Society Meeting. New Orleans, LA, USA, December 6-8, 2000. “Development and Validation of a Simple and Sensitive Immunoassay for Cadmium Ions in Environmental Water Samples”. Ibrahim A. Darwish, Diane A. Blake.

7. The 52nd Southwest/Southeast Regional American Chemical Society Meeting. New Orleans LA, USA, December 6-8, 2000. “Single-step Immunoassay for Detection of Lead in Serum”.

Ibrahim A. Darwish, R.M. Smith, D.A. Blake.

8. The 9th Annual Scientific Meeting on Molecular and Cellular Biology. Covington, LA, USA, October 21 - 22, 2000. “Production of Mouse and Rat Monoclonal Antibodies and Stability of Hybridoma Cultures in Membrane-based High Density Cell Culture Devices”. Alison M. Kriegel, Haini Yu, Ibrahim A. Darwish, James B. Delehanty, R. Srinivasan, Diane A. Blake.

9. Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy. New Orleans, LA, USA, March 4-9, 2001.

10. The 13th Annual Tulane Health Sciences Research Meeting. New Orleans, LA, USA, April 25- 26, 2001. “Anti-Chelate Monoclonal Antibodies that Exhibit Unusual Binding Characteristics”. Alison M. Kriegel, R.Mark Jones, Ibrahim A. Darwish, Diane A. Blake.

11. The 221st National Meeting of the American Chemical Society. San Diego, California, USA, April 1-5, 2001. “Determination of Cadmium in Human Serum by a Sensitive One-Step Immunoassay”. Diane A. Blake, Ibrahim A. Darwish.

12. Assiut University 3rd Pharmaceutical Sciences Conference. Assiut, Egypt, March 5-6, 2002. “ Spectroscopic Analytical Study of Certain Cephalosporins by Charge- Transfer Complexation with p-Chloranilic Acid”. Gamal A. Saleh, Hassan F. Askal, Ibrahim A. Darwish, Abdel-Nasser El- Shorbagi.

13. Assiut University 3rd Pharmaceutical Sciences Conference. Assiut, Egypt, March 5-6, 2002. “ Basic Sciences and Biotechnology Link to Protect Human Health from Environmental Hazards”. 22 Curriculum Vitae Ibrahim Darwish, Prof.

Ibrahim A. Darwish.

14. The Pharmaceutical Society of Egypt XXVIII Conference of Pharmaceutical Sciences. Cairo, Egypt, December 17-19, 2002. “Validated Spectrophotometric and Fluorimetric Methods for Analysis of Clozapine in Tablets and Urine” Hanaa M. Abdel-Wadood, Niveen M. Abdel-Latif, Ibrahim A. Darwish

15. The 18th International Congress of Clinical Chemistry and Laboratory Medicine. Kyoto, Japan, October 20-25, 2002. “On the Perspective of Capillary Electrophoresis Modes for the Determination of Morphine in Human Plasma Without Pretreatment”. Samy Emara, Ibrahim Darwish, Diaa Youssef, Tsutomu Masujima.

16. The 19th International Congress of Biochemistry and Molecular Biology. Toronto, Canada, July 20-24, 2003. "Production and Kinetic Screening of a Secondary Antibody with a Fast Association Rate". Elizabeth R. Abboud, Ibrahim A. Darwish, Diane A. Blake.

17. Assiut University 4rd Pharmaceutical Sciences Conference. Assiut, Egypt, March 6-7, 2004. “ Advances in Immunoassay Methods for Pharmaceutical Analysis”. Ibrahim A. Darwish.

18. The 9th Ibn Sina International Conference on Heterocyclic Chemistry and its Applications. Sharm El-Sheikh, Egypt, December 11-14, 2004. “Analytical Study for the Charge-Transfer Complexation Reaction of Losartan Potassium: Application to its Analysis in Presence of Hydrochlorothiazide in their Combined Dosage Forms” Ibrahim A. Darwish.

19. The Pharmaceutical Society of Egypt IXXX Conference of Pharmaceutical Sciences& Scientific Association of the Colleges of Pharmacy in the Arab World VIII Conference. Cairo, Egypt, December 21-23, 2004.

“Simple Spectrfluorimetric Method for Determination of Certain Antiviral Drugs via their Oxidation with Cerium (IV)” Ibrahim A. Darwish, Alaa Khedr, Hassan Askal, Ramadan Mahmoud.

20. Gordon Research Conference on the Cell Biology of Metals, Lewiston, ME, July 3-8, 2005. "Antibodies to Metal-Chelate Complexes: Binding Properties and Applications in the Life Sciences". D.A. Blake, H. Yu., R.C. Blake II, X. Li, I.A. Darwish, M.B. Henry

21. The First Conference of Medical Biochemistry. Assiut, Egypt, December, 21- 23, 2005. “Analysis of Certain Fluoroquinolone Antibiotics Containing Piperazine Moiety”. Hassan F. Askal, Ibrahim H. Refaat, Ibrahim A. Darwish, Mostafa A. Marzouq

22. Assiut University 4th Pharmaceutical Sciences Conference. Assiut, Egypt, March 7-8, 2006. “Spectrophotometric Determination of Certain Fluoroquinolone Antibiotics with Acidic and Alkaline Potassium Permanganate. Hassan F. Askal, Ibrahim H. Refaat, Ibrahim A. Darwish, Mostafa A. Marzouq

23. Assiut University 4th Pharmaceutical Sciences Conference. Assiut, Egypt, March 7-8, 2006.

“Simple Colorimetric Analysis of H2-Receptor Antagonists by the Reaction with N-Bromosuccinimide and Fluorescein”. Samiha A. Rahman, Ibrahim A. Darwish, Ashraf M. Mohamed, Ahmed I. Hassan.

24. International Symposium on Food and Drug Regulation: Present and Outlook, Riyadh, Saudi Arabia, May 20-22, 2006.

25. The 7th International Saudi Pharmaceutical Conference, Riyadh, Saudi Arabia, 19-21 March, 2007.

“Spectrophotometric Determination of Nizatidine and Ranitidine via Formation of their Vinylamino-Substituted Haloquinone Derivatives”. Samiha A. Rahman, Ibrahim A. Darwish, Ashraf M. Mohamed, Ahmed I. Hassan.

26. The 4th International Symposium for Development and Environment. Riyadh, KSA, March 18-20, 2008.

27. The 8th International Saudi Pharmaceutical Conference and Exhibition, Riyadh, Saudi Arabia, 26-28 April, 2010. “Novel Enzyme-Linked Immunosorbent Assay for Determination of Rosuvastatin in Plasma at Picogram Level”. Ibrahim A. Darwish, Abdul-Rahman M. Al-Obaid, Hamoud A. Al-Malaq.

Ibrahim Darwish, Prof.