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United States Patent (19) 11 E Patent Number: Re United States Patent (19) 11 E Patent Number: Re. 32,848 Berke et al. 45 Reissued Date of Patent: Jan. 31, 1989 54 ANTI-MICROBIAL REACTION PRODUCT 51) Int. Cl." ............................................ CO7D 233/60 OF #9 ALLANTON AND FORMALDEHYDE 52 U.S. C. ..................................... 548/311 (75) Inventors: Philip A. Berke, Madison; William E. 58) Field of Search ......................................... 390/548 Rosen, Summit, both of N.J. (56) References Cited 73 Assignee: Sutton Laboratories, Inc., Chatham, U.S. PATENT DOCUMENTS N.J. 3,248,285 4/1966 Berke .................................. 514/390 21 Appl. No.: 940,241 Primary Examiner-Jane T. Fan 22 Filed: Dec. 10, 1986 Attorney, Agent, or Firm-Bacon & Thomas 57 ABSTRACT Related U.S. Patent Documents N-hydroxymethyl)-N-(1,3-dihydroxymethyl-2,5-diox Reissue of: o-4-imidazolidinyl)-N'-(hydroxymethyl)urea. A reac (64) Patent No.: 4,487,939 tion product prepared by the reaction of allantoin and Issued: Dec. 11, 1984 formaldehyde is disclosed. The compound reaction Appl. No.: 214,993 product exhibits activity against bacteria, mold and yeast Fied: Dec. 10, 1980 and hence is useful as a preservative for products sus U.S. Applications: ceptable to microbial contamination. 62) Division of Ser. No. 70,502, Aug. 28, 1979, Pat. No. 4,271,176. 5 Claims, No Drawings Re. 32,848 2 though they are susceptible to deactivation. Hence, ANT-MICROBIAL REACTION PRODUCT OF #9 there exists a need for a single compound product ALLANTON AND FORMALDEHYDE which is effective against microbial contamination by yeast and mold as well as bacteria and which is easily Matter enclosed in heavy brackets I appears in the 5 incorporated into products, particularly cosmetic prod original patent but forms no part of this reissue specifica ucts, without suffering a significant reduction in activ tion; matter printed in italics indicates the additions made ity, by reissue. SUMMARY OF THE INVENTION This is a division of application Ser. No. 70,502, filed 10 Accordingly, it is an object of the invention to pro Aug. 28, 1979, now U.S. Pat, No. 4,271,176, issued June wide a compound reaction product which exhibits 2, 1981. activity against bacteria, yeast and mold and which is easily incorporated into a variety of products suscepti BACKGROUND OF THE INVENTION ble to microbial contamination without losing activity. 1. Field of the Invention 15 Another object of the invention is to provide a pre The present invention is directed to a compound servative composition containing a single compound reaction product obtained by the reaction of allantoin reaction product as the sole active ingredient against with formaldehyde. The compound reaction product bacteria, yeast or mold. exhibits activity against bacteria, mold and yeast and Still another object of the invention is to provide a hence is useful as a preservative for products susceptible method for preserving products against contamination to microbial contamination. by bacteria, mold and yeast. 2. Description of the Prior Art Other objects and advantages of the invention will be U.S. Pat. No. 3,248,285 describes a series of com evident to those of ordinary skill in the art upon review pounds prepared by condensing allantoin with formal of the detailed description of the invention contained dehyde which exhibits bacteriostatic and/or bacteri 25 cidal activity. Because of this activity, the compounds herein. are effective as preservatives in cosmetics and other DETALED DESCRIPTION OF THE products susceptible to bacterial contamination. How INVENTION ever, since cosmetics and most other perishable prod The foregoing objects and advantages are provided ucts are subject to contamination by yeast and mold as well as bacteria, the compounds must be used in combi by a compound represented by the structural formula: nation with other preservatives which are effective against yeast and mold. Such preservatives are typically CH2OH referred to as antifungal agents. O Generally, the parabens, i.e., esters of p-hydroxyben 35 HocHNHCON-CH-c1a zoic acid, are used as the additional preservative com ponents which provide protection against yeast and HOCH2N C NCH3OH mold. Although the combination of parabens with the I aforementioned bacteria-combating compounds works O well and is widely used in the cosmetic industry, it presents certain heretofore unsolved problems. In par named N-(hydroxymethyl)-N-(1,3-dihydroxymethyl ticular, the parabens, as well as a great many other 2,5-dioxo-4-imidazolidinyl)-N'-(hydroxymethyl)urea antifungal agents, are easily inactivated or deactivated. reaction product formed by condensing formaldehyde and Inactivation of parabens and like compounds may be allantoin in the proportion of four moles of formaldehyde attributed to several factors including the migration of 45 to one mole of allantoin. Unlike the numerous other the compounds from the aqueous phase of an emulsion compounds which fall within the generic structural to the oil phase, the binding of the compounds by com formula disclosed in U.S. Pat. No. 3,248,285, this com monly used nonionic emulsifiers, and/or the interfering pound reaction product is not only active against bac effect of other components in the product sought to be teria, but is also especially effective against yeast and protected. Inactivation is particularly acute in cosmet SO mold. Hence, using this compound reaction product, ics since nonionic emulsifiers inactivate antifungal it is possible to preserve formulations, especially emul agents and are common ingredients in cosmetic emul sions, which have previously been vulnerable to con sions such as creams and lotions. Thus, cosmetic formu tamination by yeast and mold even when protected by lators are often unable to adequately preserve such conventional antifungal agents. Moreover, because of emulsions against yeast or mold contamination. 55 its general effectiveness against a wide range of micro In contrast to the parabens and other antifungal bial contaminants, the compound reaction productof agents, compounds prepared in accordance with the the invention can be used as the sole preservative con disclosure of U.S. Pat. No. 3,248,285 are not inactivated ponent in perishable product formulations. by nonionic emulsifiers, do not migrate from the aque N-(hydroxymethyl)-N-(1,3-dihydroxymethyl-2,5- ous to the oil phase, and are not deleteriously affected dioxo-4-imidazolidinyl)-N'-(hydroxymethyl)urea. The by other ingredients in the various preserved products. reaction product of the invention is prepared by condens Unfortunately, the compounds heretofore prepared in ing allantoin and formaldehyde in precise quantities. accordance with the teachings of U.S. Pat. No. The reaction may be conducted in the presence of a 3,248,285 exhibit only bacteriostatic and/or bactericidal basic catalyst, such as sodium hydroxide. The reactants activity. They do not exhibit adequate antimicrobial 65 are mixed in a ratio of one mole of allantoin for every activity against yeast and mold. Parabens or similar four moles of formaldehyde optionally with the basic compounds are, therefore, still required to adequately catalyst, in an aqueous medium and heated to reflux. A protect cosmetic and other perishable products even clear, aqueous solution is obtained. Drying the solution Re. 32,848 3 4. produces the N-(hydroxymethyl)-N-(1,3-dihydrox N, 19.85%. The power was completely soluble in water ymethyl-2,5-dioxo-4-imidazolidinyl)-N'-(hydroxyme as a 30% solution. thyl)urea reaction product as a water-soluble white powder residue. (B) Preparation of Because the compound reaction product of the 5 N-(hydroxymethyl)-N-(1,3-dihydroxymethyl-2,5- invention is water-soluble, it may be readily incorpo dioxo-4-imidazolidinyl)-N'-(hydroxymethyl)urea rated into aqueous solutions or the aqueous phase of reaction product without catalysis emulsions. In solid formulations, the compound it is Addition of 1053 g of allantoin to 2160 g of 37% incorporated in powder form. The amount of the formaldehyde with stirring gave a white suspension. compound reaction product required to provide ade- 10 The suspension was stirred and heated to 85 C., and quate protection against microbial contamination will, held at that temperature for 1 hour. Cooling to room of course, vary, depending upon the particular product temperature gave a clear colorless solution of N-(hy being preserved. Those of skill in the art can easily droxymethyl)-N-(1,3-dihydroxymethyl-2,5-dioxo-4- determine dose levels in particular applications without imidazolidinyl)-N'-(hydroxymethyl)urea. Removal of undue experimentation. 15 water at reduced pressure left a white powder. The compound reaction product of the invention, thus, provides a significant improvement over similar EXAMPLE 2. preservative compounds heretofore available. Since it is Activity against yeast and mold: 0.3% test solutions. effective against bacteria, mold and yeast, it can be used A 0.5 ml. aliquot of a 24 hour A.O.A.C. yeast broth as the sole preservative component in many products. 20 culture was added to 4.5 ml. of the test solution and This eliminates the need to include antifungal preserva mixed well. The test solution was then stored at 35 C. tives such as parabens which are susceptible to deacti in an incubator for 3 days. A 0.1 ml. aliquot was asepti vation in various, common environments. The com cally removed from the test solution and placed into a pound reaction product of the invention is, therefore, a tube containing Sabourand Liquid Medium with Le dependable, effective preservative which offers signifi- 25 theen. The inoculated subculture tube was incubated at cant advantages over the individual preservatives, as 35 C. for 2 days and then examined for the presence of well as preservative combinations, currently recognized growth. and employed by those of skill in the art. The growth of a 7-10 day mold slant was washed off To further illustrate the various advantages provided with 10 ml. of sterile saline.
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