UCSF Lab Standard Operating Procedure s9

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class: Hydrazine

Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.

Type of SOP: Process Hazardous Chemical Hazardous Class

Purpose

Hydrazine is a select carcinogen and a reproductive toxin. It is air, moisture, heat, light and friction sensitive chemical. If not stored and handled properly, this can pose a serious threat to the health and safety of laboratory personnel, emergency responders and chemical waste handlers.

Common Uses: Hydrazine is mainly used as a foaming agent in preparing polymer foams, but significant applications also include its uses as a precursor to polymerization catalysts and pharmaceuticals. Additionally, hydrazine is used in various rocket fuels and to prepare the gas precursors used in air bags (Sodium azide, the gas-forming agent in air bags, is produced from hydrazine by reaction with sodium nitrite). Hydrazine is used within both nuclear and conventional electrical power plant steam cycles to control concentrations of dissolved oxygen in an effort to reduce corrosion. Hydrazine is a useful building block in organic synthesis of pharmaceuticals and pesticides. It is used as an oxygen scavenger and corrosion inhibitor in water boilers and heating systems. It is also used to reduce metal salts and oxides to the pure metals in electroless nickel plating and plutonium extraction from nuclear reactor waste. Some color photographic processes also use a weak solution of hydrazine as a stabilizing wash, as it scavenges dye coupler and unreacted silver halides. In addition, a semiconductor deposition technique using hydrazine has recently been demonstrated, with possible application to the manufacture of thin-film transistors used in Liquid Crystal Displays (LCD systems).

Physical & Chemical Properties/Definition of Chemical Group

Hydrazine 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) CAS# - 302-01-2

Physical State: Liquid Appearance: Colorless Odor: Strong odor - Ammonia-like Vapor Pressure: 10 mm Hg @ 20 deg C Vapor Density: 1.1 (air=1) Viscosity: 0.90 Boiling Point: 113 deg C Freezing/Melting Point: 1.4 deg C Solubility: Soluble Specific Gravity/Density: 1.01 (Water=1)

Molecular Formula: N2H4 Molecular Weight: 32.05

Potential Hazards/Toxicity

Danger!

Strong reducing agent

Fire and explosion risk in contact with oxidizing agents

May be fatal if absorbed through the skin

Causes eye and skin burns

Causes digestive and respiratory tract burns

Flammable liquid and vapor

Harmful if inhaled or swallowed

May cause allergic skin reaction

Cancer suspect agent

May cause blood abnormalities

May cause liver and kidney damage

Potential Health Effects

Eye: May cause irreversible eye injury. Exposure to the vapors or liquid may cause temporary blindness. Causes severe eye irritation and burns.

Skin: May be fatal if absorbed through the skin. Prolonged and/or repeated contact may cause irritation and/or dermatitis. May cause skin sensitization, an allergic reaction, which becomes evident upon re- exposure to this material. Contact with liquid is corrosive and causes severe burns and ulceration. Contact with the skin may dissolve hair.

Hydrazine 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Ingestion: Harmful if swallowed. Causes gastrointestinal irritation with nausea, vomiting and diarrhea. May cause liver and kidney damage. Causes digestive tract burns with immediate pain, swelling of the throat, convulsions, and possible coma. Exposure may cause anemia and other blood abnormalities.

Inhalation: Harmful if inhaled. Irritation may lead to chemical pneumonitis and pulmonary edema. May cause liver and kidney damage. Causes severe irritation of upper respiratory tract with coughing, burns, breathing difficulty, and possible coma. Vapors may cause dizziness, nausea, itching, burning, and swelling of the eyes.

Chronic: Repeated inhalation may cause chronic bronchitis. May cause cancer according to animal studies. Repeated exposure may cause sensitization dermatitis. May cause reproductive and fetal defects.

Permissible Exposure Limit (PEL) - OSHA Vacated PELs: 0.1 ppm TWA; 0.1 mg/m3 TWA

National Fire Protection Association – NFPA ratings:

Health: 3

Flammability: 3

Reactivity: 3

Engineering Controls

Store & handle inside a glove box (under an inert atmosphere – preferably under Nitrogen). For additional information, please refer to the MSDS (i.e., sections on ‘Stability & Reactivity’ and ‘Handling & Storage’) from the same manufacturer.

Personal Protective Equipment (PPE)

Respiratory protection

If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand protection

Handle with Neoprene/Nitrile gloves.

Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf

OR http://www.allsafetyproducts.biz/page/74172

Hydrazine 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) OR http://www.showabestglove.com/site/default.aspx

Gloves must be inspected prior to use.

Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with hydrazine.

Wash with warm water and soap, dry hands.

Eye protection

Safety goggles.

Skin and body protection

Fire resistant lab coat (100% cotton based)

Cotton based clothing/attire.

Full length pants

Close toed shoes

Hygiene measures

Avoid contact with skin, eyes and clothing.

Wash hands before breaks and immediately after handling hydrazine.

First Aid Procedures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately. Do NOT allow victim to rub eyes or keep eyes closed.

Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Discard contaminated clothing in a manner which limits further exposure.

Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.

Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. Do NOT use mouth-to-mouth resuscitation. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask.

Hydrazine 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Notes to Physician: Treat symptomatically and supportively.

Special Handling and Storage Requirements

Handling

 Wash thoroughly after handling.  Remove contaminated clothing and wash before reuse.  Ground and bond containers when transferring material (i.e., if in a metal container). For safety reasons, transfer from glass to glass container using proper techniques (often purged with nitrogen).  Never add water to this product.  Avoid shock and friction.  Use spark-proof tools and explosion proof equipment.  Do not get in eyes, on skin, or on clothing.  Empty containers retain product residue, (liquid and/or vapor), and can be dangerous.  Keep container tightly closed.  Do not ingest or inhale.  Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames.  Keep away from heat, sparks and flame.  Use only with adequate ventilation or respiratory protection.

Storage

 Keep locked up. Keep container dry.  Keep away from heat, sparks & flame.  Keep away from sources of ignition.  Keep away from acids and moisture.  Do not store in direct sunlight or strong incandescent light.  Storage under a nitrogen blanket has been recommended OR inside a glove box (under inert atmosphere).  Isolate from oxidizing materials and acids.

 All hydrazine containers must be stored using secondary container (tray/tub) with proper signage/caution label.  Hazard communication: Warning/Caution label is required on each hydrazine container, secondary containment and designated storage cabinets/areas. Warning/Caution label must state the following:

“CARCINOGEN / CANCER HAZARD or SUSPECT CANCER AGENT & Reproductive Toxin”

Chemical Stability: Thermally unstable.

Conditions to Avoid: Light, ignition sources, moisture, temperatures above 150°C.

Hydrazine 5 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Incompatibility

 Substance is highly reactive reducing agent.  Incompatible with oxidizing agents (including air), acids, and some metal oxides and metals. Substance may spontaneously ignite in air when in contact with porous materials.  Ignites on contact with dinitrogen oxide and tetroxide, hydrogen peroxide, tetryl, and nitric acid. Explodes on contact with dicyanofurazan, n-halomides, potassium, silver compounds, sodium hydroxide, titanium compounds, and trioxygen difluoride.  Explosive compounds may result from contact with air - chloromethylnitrobenzene, lithium perchlorate, metal salts, methanol + nitromethane, sodium, and sodium perchlorate.  Also incompatible with barium oxide or calcium oxide, benzeneseleninic acid or anhydride, calcium, carbon dioxide + stainless steel, 1-chloro-2,4-dinitrobenzene, cotton waste + heavy metals, (difluoroamino) difluoroacetonitrile, iodine pentoxide, rust, ruthenium(III) oxide, thiocarbonyl azide thiocyanate,

Hazardous Decomposition Products: Oxides of nitrogen, ammonia and/or derivatives.

Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Hydrazine 6 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP: ______(g , ml) to ______(g, ml)

Temperature range covered by this SOP: __ °C – __ °C

General Overview and Purpose: Enter the experimental purpose

Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

Any deviation from this SOP requires approval from the Principal Investigator.

Hydrazine 7 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)