Identification of RFLP and NBS/PK profiling markers for disease resistance loci in genetic maps of oats
MJ Sanz1, Y. Loarce1, A. Fominaya1, J. Vossen2, E. Ferrer1*
1 Department of Cell Biology and Genetics, University of Alcalá, Campus Universitario, 28871 Alcalá de Henares, Madrid (Spain) 2 Wageningen UR Plant Breeding, Wageningen University and Research Centre, Drovendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Supplementary Table S5. Fragments obtained from cDNA NBS/PK profiling bands from A. strigosa (Sg ), A. wiestii (Wt), MN841801-1 (Mn), Noble-2 (N) with significant identity to R genes, protein kinase genes or RGA sequences. The last nine sequences showed identity to protein sequences involved in metabolism or in other cellular processes.
Sequence Enz/primer P Size Blastx Similarity GenBank % Id. name accesión No. cDNASg1.3.1 AluI/PK1Fa - 536 pb Receptor ser/thr Kin PR5K-like (Oryza) BAD01755 75 cDNASg2.1 AluI/PK1Fa - 488 pb Wall-associated protein kinase (Oryza) AAX95007 67 cDNAWt3.1 AluI/PK1Fa - 432 pb Receptor ser/thr Kin PR5K-like (Oryza) BAD82483 73 cDNASg7.3 AluI/PK1Fa - 349 pb Protein kinase APK1B (Zea mays) NP_001150204 97 cDNAWt8.3 AluI/PK1Fa - 329 pb Putative receptor sert/thr kinase PR5K (Oryza) BAD61470 82 cDNASg9.1 AluI/PK1Fa + 313 pb Kinase R-like protein (Triticum) AAL51069 85 cDNASg9.3 AluI/PK1Fa + 313 pb Putative protein kinase (Zea) CAH39852 87 cDNAWt13.3.3 MseI/NBS5A + 300 pb Putative NBS-LRR-prot1 (Oryza) BAD36467 48 cDNASg17.7.3 MseI/PK4R1b - 355 pb SERK2 (Helianthus) AAL93162 92 cDNASg18.2 MseI/PK4R1b + 267 pb Receptor kinase ORK45 (Avena sativa) AAM09950 92 cDNAWt19.8.2 MseI/PK4R1b - 265 pb Ser/thr kinase (Glycine max) ACM89544 88 cDNASg28.6 AluI/NBS6 - 322 pb Protein resistance NBS-LRR (Avena strigosa) CAC10049 100 cDNASg23.6.1 AluI/NBS6 + 236 pb MLA27-2 (Hordeum) ACZ65496 55 cDNAMn42.2 MseI/NBS5A - 279 pb Putative rp3 disease resistance protein (Zea) AAN23088.1 56 cDNAN40.1 MseI/NBS5A - 426 pb Putative rp3 disease resistance protein (Zea) AAN23088.1 55 cDNAMn41.1 MseI/NBS5A - 300 pb Putative NBS-LRR (Oryza) AAT81724 48 cDNAMn41.3 MseI/NBS5A - 300 pb Putative NBS-LRR (Oryza) AAT81724 50 cDNAMn43.4 MseI/NBS5A - 255 pb Putative NBS-LRR (Oryza) AAT81724.1 48 cDNAMn52.4 MseI/PK3Fb - 675 pb Putative receptor LRK1 (Oryza) BAD08845.1 58 cDNAMn52.2 MseI/PK3Fb - 677 pb Putative receptor LRK1 (Oryza) BAD08845.1 49 cDNAN50.1 MseI/PK3Fb - 631 pb Putative protein kinase (Oryza) AAM14696.1 62 cDNAN50.3 MseI/PK3Fb - 629 pb Putative receptor like ser/thr kin RFK1 (Oryza) BAD03117.1 68 cDNAMn53.2 MseI/PK3Fb - 565 pb Putative kinase (Zea mays) NP001147903.1 85 cDNAMn35.3 RsaI/PK3Fb + 283 pb Receptor like protein kinase (Oryza) BAC57713.1 81 cDNAMn45.2 MseI/NBS6 + 255 pb MLA-27-2 (Hordeum) ACZ65496.1 53 cDNAMn54.1 MseI/PK3Fb - 432 pb Putative receptor LRK1 (Oryza) BAD09243.1 62 cDNAN6.2 MseI/NBS6 + 230 pb MLA-27-2 (Hordeum) ACZ65496.1 55 cDNAMn20.1 MseI/PK4R1b - 363 pb Ser/thr prot kinase prot 1 (Triticum) ABC54583.1 100 cDNAMn20.3 MseI/PK4R1b - 355 pb SERK1 (Oryza) AAU88198.1 95 cDNAMn20.4 MseI/PK4R1b - 361 pb Ankryin repeat S-palmotoyl transferase (Oryza) ABA94184 80 cDNAN39.1 MseI/NBS5A - 489 pb NAD dependent epimerase/dehidratase (Ricinus) XP_002512541 91 cDNAMn56.2 MseI/PK3Fb - 335 pb Actina (Triticum) ACU27919 99 cDNAMn35.2 RsaI/PK3Fb + 286 pb NADH-ubiquinone oxidoreductase (Zea) NP001149378.1 90 cDNASg30.6 MseI/GLPL - 256 pb Peptide methionine sulfoxide reductase (Secale) CAE92372 91 cDNASg29.6 AluI/NBS6 - 301 pb Type-IIIa membrane protein cp-wap13 (Vigna) AAB61672 91 cDNAN36.6 MseI/GLPL - 782 pb Putative elicitor-inducible cytP450 (Oryza) BAD36163 78 cDNAMn49.2 RsaI/PK1Fa - 378 pb Putative short dehydrogenase/reductase (Oryza) BAD32915 83 cDNAMn47.3 RsaI/PK1Fa - 523 pb Ser/thr protein phosphatase 2A (PP2A) BAD67828 85