OIE Reference Laboratory Reports s11

OIE Reference Laboratory Reports Activities in 2011

Name of disease (or topic) for Taura syndrome which you are a designated OIE Reference Laboratory:

Address of laboratory: Aquaculture Pathology Laboratory Department of Veterinary Science and Microbiology University of Arizona Building 90, Room 202 Pharmacy/Microbiology Tucson, AZ 85721 UNITED STATES OF AMERICA

Tel.: (1.520) 621.84.14

Fax: (1.520) 621.48.99

e-mail address: [email protected]

website: http://microvet.arizona.edu/research/aquapath/index.htm

Name (including Title and Dr Donald V. Lightner Position) of Head of Laboratory Professor (Responsible Official):

Name(including Title and Dr Donald V. Lightner Position) of OIE Reference Professor Expert:

Name (including Title and Dr Donald V. Lightner Position) of writer of this report & Linda Nunan, Assistant Staff Scientist (if different from above):

Annual reports of OIE Reference Centres, 2011 1 Taura syndrome

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Test For Specificity Total

PCR Surveillance & SPF All known TS viral 1412 verification genotypes

Histology Initial diagnosis & Lesions 2696 surveillance

ISH Confirmatory diagnosis All known TS viral 7 genotypes

Real-time PCR Confirmatory diagnosis & All known TS viral 27 quantification of viral load genotypes

2. Production and distribution of diagnostic reagents

Date Country Material provided Quantity sent

05/26/2011 Mexico TSV-infected tissue 1 vial

05/31/2011 Argentina TSV-infected tissue 1 vial

06/01/2011 Brazil TSV + control plasmid 1 vial

06/01/2011 Saudi Arabia TSV-infected tissue 1 vial

06/11/2011 India TSV-infected tissue 1 vial

07/14/2011 Malaysia TSV-infected tissue 1 vial

09/22/2011 Ecuador TSV-infected tissue 1 vial

09/29/2011 Vietnam TSV-infected tissue 1 vial

10/06/2011 Singapore TSV + control plasmid 1 vial

10/06/2011 Chinese Taipei TSV-infected tissue 1 vial

11/23/2011 Saudi Arabia (3) TSV-infected tissue; geographic isolates 3 vials

2 Annual reports of OIE Reference Centres, 2011 Taura syndrome

Part II: Activities specifically related to the mandate of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results

b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results

Ring trials: Taura syndrome virus detection by RT-PCR

Country Month & Year No. Labs participating Correct results

Ecuador August 2011 1 YES

Madagascar February 2011 1 YES

August 2011 1 YES

Saudi Arabia February 2011 1 NO

Mexico February 2011 7 Lab 1: YES Lab 2: YES Lab 3: YES Lab 4: YES Lab 5: NO Lab 6: N-An Lab 7: N-An

August 2011 5 Lab 1: YES Lab 2: YES Lab 3: YES Lab 4: YES Lab 5: YES

Panama February 2011 1 YES

India August 2011 1 NO

Vietnam February 2011 2 Lab 1: YES Lab 2: YES

August 2011 1 YES

N-An = not analyzed

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

See section Part I-2  Taura syndrome virus infected shrimp tissue  Plasmid DNA for use as positive control in RT-PCR reactions

5. Research and development of new procedures for diagnosis and control

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned

 Quality assurance of sample material provided is achieved by pre-testing the sample material for the pathogen of interest, by PCR, prior to shipment.  Biosafety and biosecurity is achieved by following federal regulations for disposal of infectious materials and through the inland location of the laboratory, as no marine pathogens would be able to survive/remain infectious in the natural environment of the Sonora Desert.

8. Provision of consultant expertise to OIE or to OIE Member Countries

Chapters for the OIE Aquatic Animal Health Code – assisted in preparation Lightner DV. 2011. Aquatic Animal Health Code, 14th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris: - Infectious hypodermal and haematopoietic necrosis, Chapter 9.2. - Infectious myonecrosis, Chapter 9.3. - Necrotizing Hepatopancreatitis, Chapter 9.4. - Taura syndrome, Chapter 9.5. - White spot disease, Chapter 9.6. - White tail disease, Chapter 9.7. - Yellow head disease, Chapter 9.8.

Chapters for the OIE Manual of Diagnostic Tests for Aquatic Animals Lightner DV. In press (2012). Diagnostic Manual for Aquatic Animal Diseases, 7th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris: - Disinfection of Crustacean Farms, Section C of Methods for Disinfection of Aquaculture Establishments. - Diseases of Crustaceans, General Information. - Infectious Hypodermal and Hematopoietic Necrosis. - Infectious Myonecrosis. - Taura Syndrome. - Tetrahedral Baculoviruosis (Baculovirus penaei). - Spherical Baculovirosis (Penaeus monodon-type baculovirus).

Advisor to the Aquatic Animal Health Standards Commission, Office International Des Epizooties, Paris. Term 2009-2011, Advisor (~10 days/year). I was appointed to a third 3-year term by the OIE Central Bureau, beginning in May 2009, on the OIE Aquatic Animal Health Standards Commission (AAHSC) which meets 2 times per year to consider new diagnostic methods for OIE listed and notifiable aquatic animal diseases; to consider changes to the OIE Aquatic Animal Code; and to make recommendations to the Director General and to the OIE World Assembly of Member Country Delegates. In 2011, I participated in the AAHSC meeting of October 3-7 at the OIE Headquarters in Paris.

9. Provision of scientific and technical training to personnel from other OIE Member Countries

• Hosted Borlaug scholar Mr Bambang Hanggono, of the Indonesian Government’s Situbondo Laboratory. Mr Hanggono visited the Aquaculture Pathology Laboratory for 12 weeks (17 September to 3 December, 2011). Mr Hanggono’s training emphasized histopathology and molecular diagnostic tests for major shrimp diseases including Taura syndrome virus.

10. Provision of diagnostic testing facilities to other OIE Member Countries

The UAZ lab received and tested confirmatory samples from the Kingdom of Saudi Arabia for TSV.

11. Organisation of international scientific meetings on behalf of OIE or other international bodies

12. Participation in international scientific collaborative studies

• Development of qPCR kits with Life Technologies of Singapore & USA. Provided samples of frozen shrimp tissue infected with IMNV and genome sequence information to Life Technologies (formerly Applied Biosystems) of Singapore & USA for development of qPCR/RT-PCR test kits.

In late 2011, we received a real time thermocycler for use in validating shrimp virus kits for Life Technologies.

13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

 Presentations at international conferences and meetings

Invited speaker OECD Symposium, Halifax, Canada. Given by OECD Co-operative Research Program, Paris, 8/7/2011.

Invited keynote speaker, Honduras. Given by V Simposio Nicovita, Honduras, 11/24/2011. (100%).

Invited keynote speaker, Ecuador. Given by XIII Congreso Ecuatoriano de Acuicultura and Aquaexpo, 10/20/2011.

Invited speaker - OIE Global Conference on Aqiuatic Animal Health, Panama. Given by OIE , 6/29/2011.

Invited keynote speaker, Panama City, Panama. Given by El Comite Organizador del X Simposio International de Camaron de Cultivo y Exhibicion Comercial, 5/13/2011.

 Scientific publications in peer-reviewed journals

Lightner D.V. 2011. Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review. Journal of Invertebrate Pathology 106: 110-130.

Lightner, D.V. 2011. Status of shrimp diseases and advances in shrimp health management. pp. 121-134, in M.G. Bondad-Reantaso, J.B. Jones, F. Corsin and T. Aoki (editors), Diseases in Asian Aquaculture VII. Fish Health Section, Asian Fisheries Society, Selangor, Malaysia. 385 p.

Bakonyi, T., B.C. Bonning, D.V. Lightner, Y.J. Chen, N. Nakashima, S.M. Valles and P. Christian. 2012. Dicistroviridae. In A.M.Q. King, M.J. Adams, E.B. Carstens and E.J. Lefkowitz, editors: Virus Taxonomy. 9th Report of the International Committee on Taxonomy of Viruses, Oxford, Elsevier, pp. 840 - 845.

Aranguren L.F., M. Salazar, K. Tang and D. Lightner. In Press. Characterization of a highly pathogenic strain of Taura syndrome virus (TSV) from Colombian shrimp farms. J. Virus Research.

Lightner, D.V. and R.M. Redman. In Press. Development of specific pathogen-free (SPF) shrimp stocks and their application to sustainable shrimp farming. Woodhead Publishing, LTD., Cambridge. UK.

Lightner DV. "In press" 2012. Diagnostic Manual for Aquatic Animal Diseases. 7th Edition. World Organization for Animal Health ( Office International des Epizooties = OIE), Paris:

- Taura Syndrome

Aranguren L.F., K. Tang and D. Lightner. Submitted. Protection from yellow head virus (YHV) infection in Penaeus vannamei pre-infected with Taura syndrome virus (TSV). Diseases of Aquatic Organisms.

 Other communications

Popular Press

Lightner, D.V. 2011. Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. . Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. Panorama Acuícola 16(4): 8-20.

Arce, S.M., S.M. Moss and D.V. Lightner. 2011. Biosecurity principles for sustainable production using SPF shrimp. Global Aquaculture Advocate. May/June 2011, 14-16.

Pantoja, C.R., S.A. Navarro and D.V. Lightner. 2011. Ring test compare PCR results from diagnostic laboratories. Global Aquaculture Advocate. May/June 2011, 36-37.

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Annual reports of OIE Reference Centres, 2011 7