Before Sending Your Order to 1St BASE, Please Check That You Have

Axil Scientific Pte Ltd First BASE Laboratories Sdn Bhd (604944-X) Molecular Biology Services 41 Science Park Road, #01-22/23 The Gemini Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, PCR Optimization Order Form Singapore Science Park II, Singapore 117610 Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia T: +65 6775 7318 T: +603 8943 3252 F: +65 6775 7211 F: +603 8943 3243 E: [email protected] E: [email protected]

*Required Fields CUSTOMER DETAILS *Name:

Reference ID: *Institute Name / Department *Address:

*Contact Number: (Office) (Mobile) *Email Address: Principal Investigator / Supervisor:

TYPE OF SERVICE (please choose ONE only) Please tick ( √ ) Service Name Description

SERVICE A. - PCR optimization WITH Primer Design Primers will be designed and synthesized at 1st BASE

SERVICE B - PCR optimization WITHOUT Primer Design Primer sequences will be submitted by customer for synthesis at 1st BASE

of 4 Axil Scientific Pte Ltd First BASE Laboratories Sdn Bhd (604944-X) Molecular Biology Services 41 Science Park Road, #01-22/23 The Gemini Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, PCR Optimization Order Form Singapore Science Park II, Singapore 117610 Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia T: +65 6775 7318 T: +603 8943 3252 F: +65 6775 7211 F: +603 8943 3243 E: [email protected] E: [email protected]

Service A – PCR Optimization WITH Primer Design Target DNA Template No. GenBank Region Name Size No. Name Organism Type of Conc. Purity Vol. Accession (Start..End) (bp) DNA (ng/l) OD260/280 (l) Number □ gDNA □ cDNA

1 1 2 3 4 5 2 1 2 3 4 5 3 1 2 3 4 5

Service B – PCR Optimization WITHOUT Primer Design PCR Primers DNA Template PCR product No Primer Name Tm (oC) No. Name Organism Type of Conc. Purity Vol. Name Size DNA Sequence (5’ to 3’) (ng/g) OD260/280 (l) (bp)

1 Forward 1 Insert sequence 2 Reverse 3 Insert sequence 4 5 2 Forward 1 Insert sequence 2 Reverse 3 Insert sequence 4 5 3 Forward 1 Insert sequence 2 Reverse 3 Insert sequence 4 5

Add-on Services (Note: additional fees are applicable, please submit DNA Sequencing Order Form or PCR Production Cloning Submission Form separately) Quantity Type of Services

Blunting of 3’dA-overhang PCR product

Sequencing of PCR product (bi-directional)

PCR product cloning

Before sending your order to 1st BASE, please check that you have:

 Prepared ≥ 3 – 5 µg DNA template in either TE or 10mM Tris-HCl (pH8.0) Elution Buffer

 Purified DNA meets OD260/280= 1.8 to 2.0  Prepared ≥ 100 µl of 10 µM primer in 1x TE buffer  Prepared samples in 1.5ml microcentrifuge tubes with at least 10 µl of contents. Each tube should be clearly labeled using a permanent marker, with caps sealed with parafilm. DNA should be shipped 4oC.

Optional: If PCR Optimization has been done before, please attach PCR setup, thermal cycling condition, labelled gel photo for our reference