UCSF Lab Standard Operating Procedure s37

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class: Fowler’s Solution

Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Fowler’s solution is a 1% solution of potassium arsenite. Potassium arsenite is classified by IARC as Group 1, carcinogenic to humans. Side effects from prolonged exposure include liver cirrhosis, urinary bladder cancer, and skin cancers. Symptoms of arsenic poisoning may result from exposure to this chemical. Fowler’s solution is named after the doctor in England who proposed its use in 1786. It was prescribed in the United States until the 1950s to treat a range of ailments including malaria, chorea, and syphilis but was banned due to its toxicity.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 1332-10-1

Class: Carcinogen, toxic

Molecular Formula: KH2AsO3

Form (physical state): Liquid

Color: Colorless

Boiling point: N/A

Potential Hazards/Toxicity

Fowler’s Solution 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Fowler’s solution is a 1% solution of potassium arsenite. Potassium arsenite is classified by IARC as Group 1, carcinogenic to humans. It is toxic if inhaled or ingested. It may be harmful if absorbed through the skin. It may cause respiratory tract, gastrointestinal tract, skin, and eye irritation. Symptoms of exposure include burning, coughing, headache, muscle cramps/spasms, nausea, vomiting, diarrhea, shock, and death. Prolonged exposure may cause dermatitis. Exposure may affect the peripheral nervous system, mucous membranes, bone marrow, kidneys and liver, resulting in neuropathy, pigmentation disorders, perforation of nasal septum, lesions of blood cells, kidney impairment, and cirrhosis. Chronic exposure to inorganic arsenic compounds can cause skin, liver, and lung cancer. Inorganic arsenic-containing compounds have a permissible exposure limit (PEL) of 10 µg/m3.

Engineering Controls

Chemical fume hood. Adequate exhaust and capture filtration.

Personal Protective Equipment (PPE)

Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand Protection Handle with gloves. Nitrile gloves are recommended.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Fowler’s solution.

Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/

Eye Protection ANSI approved, tight-fitting glasses/goggles. Face shields are recommended.

Skin and Body Protection Appropriately fitting lab coat, long pants, closed-toe shoes.

Hygiene Measures Avoid contact with skin, eyes, and clothing. Wash hands before breaks and after handling.

First Aid Procedures

If inhaled Move person into fresh air. If not breathing, give artificial respiration. Consult a physician.

In case of skin contact Wash off with soap and plenty of water for at least 15 minutes while removing contaminated clothing. Take victim immediately to hospital.

Fowler’s Solution 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) In case of eye contact Flush eyes with plenty of water for at least 15 minutes. Consult a physician.

If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.

Special Handling and Storage Requirements

Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion. Provide adequate exhaust ventilation. Ensure normal measures for preventative fire protection.

Conditions for safe storage: Keep container tightly closed in a dry and well-ventilated area. Opened containers must be carefully resealed and kept upright to prevent leakage. Incompatible with strong oxidizing agents and strong acids.

Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Fowler’s Solution 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP: ______(g , ml) to ______(g, ml)

Temperature range covered by this SOP: __ °C – __ °C

General Overview and Purpose: Enter the experimental purpose

Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

Any deviation from this SOP requires approval from the Principal Investigator.

Fowler’s Solution 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)