Table S1. Clinical Characteristics of Patients Excluded Due to Loss of Follow up and Incomplete

Table S1. Clinical characteristics of patients excluded due to loss of follow up and incomplete laboratory data (n=3439) and patients included in the current analysis (n=4080). Excluded Included Characteristics p-value n = 3439 n = 4080

Demographics age [years] 58.4 ± 15.4 58.9 ± 13.4 0.14 ≥ 65 years, n (%) 1291 (37.6) 1476 (36.2) 0.21 Female gender, n (%) 1981 (57.6) 2457 (60.2) 0.02 Smoker, n (%) 427 (18.8) 630 (15.4) < 0.01

Clinics Waist [cm] 95.6 ± 14.5 95.3 ± 15.3 0.48 BMI [kg/m²] 27.4 ± 5.0 27.4 ± 5.1 0.59 ≥ 25 kg/m², n (%) 711 (33.0) 1414 (34.7) 0.19 Hypertension, n(%) 1179 (47.8) 1915 (46.9) 0.52 BP systolic [mmHg] 133.4 ± 18.6 132.4 ± 18.3 0.03 BP diastolic [mmHg] 80.1 ± 9.7 79.8 ± 9.4 0.13 Coronary heart disease, n (%) 460 (18.6) 562 (13.8) 0.01 Diabetes mellitus, n (%) 520 (21.1) 847 (20.8) 0.77 HbA1c [mg/dL] 5.5 (5.2-5.8) 5.5 (5.2-5.8) 0.83 Hyperlipidaemia, n (%) 1058 (42.9) 1754 (43.0) 0.93 LDL-C[mg/dL] 127.3 ± 34.2 126.1 ± 32.4 0.20 CKD MDRD 767 (38.3) a 1474 (36.1) 0.09 eGFR MDRD [mL/min/1.73m²] 65.1 ± 17.5 a 66.0 ± 15.9 0.56 CKD CKD-EPI 613 (30.6) a 1139 (27.9) 0.03 eGFR CKD-EPI [mL/min/1.73m²] 69.5 ± 19.1 a 70.9 ± 17.6 0.01 a Creatinine plasma concentration available in n= 3170 participants. A random sample of 55,518 patients was recruited on two pre-defined half-days in September 2003. Thereof, a representative sample of 7519 subjects received comprehensive laboratory examinations. This sample was prospectively followed until end of 2007. A total of 3439 patients had to be excluded because they were lost to follow up (n=3170) or because one or more items required for the calculation of the eGFR-MDRD or the eGFR-CKD-EPI (n = 269). This left 4080 patients for the analyses. Continuous data are presented as means ± standard deviations or medians with interquartile ranges; categorical variables as absolute and relative frequencies. Smoker, use of any tobacco product in the last 4 weeks; BMI, body mass index; Hypertension, systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg; HbA1c, glycated haemoglobin A1c; diabetes mellitus, HbA1c ≥ 6.5% and/or fasting glucose ≥ 126 mg/dL; LDL-C, low density lipoprotein cholesterol; hyperlipidaemia, LDL-C ≥ 115 mg/dL, CKD, chronic kidney disease; eGFR MDRD, glomerular filtration rate estimated with the Modification of Diet in Renal Disease (MDRD) equation; eGFR CKD-EPI, glomerular filtration rate estimated with the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation.

Table S2. Prevalence of eGFR categories estimated with the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula in 2004.

Age 18-29 30-39 40-49 50-59 60-69 70-79 80+ Total N 52 391 744 855 1145 763 130 4080 eGFR ≤ 29 0.0 0.0 0.0 0.4 0.5 1.6 6.2 0.7 30 – 44 0.0 0.5 0.5 2.6 4.6 13.8 21.5 5.2 45 – 59 1.9 8.7 12.8 19.8 26.4 32.5 36.2 22.0 ≥ 60 98.1 90.8 86.7 77.3 68.5 52.2 36.2 72.1 Age (years); N, number of participants in each age category; eGFR (mL/min/1.73m²); all entries are column percentages.

Table S3. Prevalence of eGFR categories estimated with the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula in 2007.

Age 18-29 30-39 40-49 50-59 60-69 70-79 80+ Total N 52 391 744 855 1145 763 130 4080 eGFR ≤ 29 0.0 0.0 0.1 0.3 1.0 2.5 5.9 1.3 30 – 44 0.0 0.0 0.5 1.6 4.8 18.0 29.6 7.9 45 – 59 0.0 5.3 8.8 17.3 32.8 38.3 44.6 25.8 ≥ 60 100 94.7 90.5 80.7 61.5 41.3 19.9 65.0 Age (years); N, number of participants in each age category; eGFR (mL/min/1.73m²); all entries are column percentages.

Table S4. Prevalence of eGFR categories estimated with the Modification of Diet in Renal Disease (MDRD) formula for 2004.

Age 18-29 30-39 40-49 50-59 60-69 70-79 80+ Total N 52 391 744 855 1145 763 130 4080 eGFR ≤ 29 0.0 0.0 0.1 0.4 0.6 1.4 4.6 0.6 30 – 44 0.0 1.3 2.2 4.7 6.5 14.2 23.1 6.7 45 – 59 19.2 18.9 27.0 30.8 27.3 34.7 36.2 28.8 ≥ 60 80.8 79.8 70.7 64.2 65.6 49.7 36.2 63.9 Age (years); N, number of participants in each age category; eGFR (mL/min/1.73m²); all entries are column percentages. Table S5. Prevalence of eGFR categories estimated with the Modification of Diet in Renal Disease (MDRD) formula for 2007.

Age 18-29 30-39 40-49 50-59 60-69 70-79 80+ Total N 52 391 744 855 1145 763 130 4080 eGFR ≤ 29 0.0 0.0 0.1 0.5 1.0 2.4 3.8 1.1 30 – 44 0.0 1.6 1.8 3.7 7.1 18.0 31.0 9.3 45 – 59 3.6 15.9 27.0 32.9 35.2 40.3 44.9 34.0 ≥ 60 96.4 82.5 71.1 62.9 56.8 39.4 20.2 55.6 Age (years); N, number of participants in each age category; eGFR (mL/min/1.73m²); all entries are column percentages.

Table S6. Baseline characteristics according to sex in 2004 and 2007 p-value men women (men/women) Characteristics 2004 2007 2004 2007 2004 2007 age, years 60.0 ± 12.0 63.0 ± 12.0 58.0 ± 14.0 61.0 ± 14.0 0.01 0.01 <45 y, n (%) 232 (14.3) 168 (10.4) 560 (22.8) 418 (17.0) 0.01 0.01 45-65 y, n (%) 752 (46.3) 649 (40.0) 1060 (43.1) 983 (40.0) 0.04 1.00 65-75 y, n (%) 480 (29.6) 530 (32.7) 561 (22.9) 641 (26.1) 0.01 0.01 75+ y, n (%) 159 (9.8) 276 (17.9) 276(11.2) 415 (16.9) 0.15 0.93 Waist [cm] 103.0 ± 13.0 104.0 ± 12.0 90.0 ± 15.0 91.0 ± 15.0 0.01 0.01 BMI [kg/m²] 28.1 ± 4.3 28.2 ± 4.5 27.1 ± 5.5 27.2 ± 5.5 0.01 0.01 Smoker, n (%) 278 (17.3) 216 (14.8) 352 (14.4) 281 (12.6) 0.02 0.05 Hypertension, n (%) 830 (51.1) 940 (57.9) 1085 (44.2) 1203 (49.0) 0.01 0.01 BP systolic [mmHg] 135 ± 16 134 ± 16 131 ± 19 131 ± 18 0.01 0.01 BP diastolic [mmHg] 81 ± 9 80 ± 9 79 ± 10 79 ± 10 0.01 0.01 CHD, n (%) 321 (19.8) 391 (24.1) 241 (9.8) 271 (11.0) 0.01 0.01 Diabetes mellitus, n (%) 423 (26.1) 471 (29.1) 424 (17.3) 480 (19.5) 0.01 0.01 HbA1c [mg/dL] 5.7 ± 0.8 5.8 ± 0.9 5.6 ± 0.7 5.7 ± 0.7 0.01 0.01 Hyperlipidaemia, n (%) 795 (49.0) 834 (51.4) 959 (39.0) 1050 (42.7) 0.01 0.01 LDL-C [mg/dL] 123 ± 23 136 ± 38 128 ± 32 144 ± 38 0.01 0.01 CKD MDRD, n (%) 469 (28.9) 540 (33.3) 1005 (40.9) 1273 (51.8) 0.01 0.01 eGFR MDRD [mL/min/1.73m²] 69.3 ± 16.2 65.0 ± 13.6 63.8 ± 15.3 58.8± 12.2 0.01 0.01 CKD CKD-EPI, n (%) 381 (23.5) 454 (28.0) 758 (30.9) 972 (39.6) 0.01 0.01 eGFR CKD-EPI [mL/min/1.73m²] 72.8 ± 17.3 68.0 ± 15.6 69.7 ± 17.7 63.7 ± 15.0 0.01 0.01 Continuous data are presented as means ± standard deviations or medians with interquartile ranges; categorical variables as absolute and relative frequencies; p-values, comparison between men and women in 2004 and 2007, respectively. Smoker, use of any tobacco product in the last 4 weeks; BMI, body mass index; Hypertension, systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg; CHD, coronary heart disease; HbA1c, glycated haemoglobin A1c; diabetes mellitus, HbA1c ≥ 6.5% and/or fasting glucose ≥ 126 mg/dL; LDL-C, low density lipoprotein cholesterol; hyperlipidaemia, LDL ≥ 115 mg/dL, CKD, chronic kidney disease; eGFR MDRD, glomerular filtration rate estimated with the Modification of Diet in Renal Disease (MDRD) equation; eGFR CKD-EPI, glomerular filtration rate estimated with the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation.