Sveučilište U Splitu Medicinski Fakultet

University of Split School of Medicine Department of Medical Microbiology and Parasitology MEDICAL STUDIES IN ENGLISH

Detailed schedule of teaching units: lectures, seminars and practicals for the subject “Basics of Medical Microbiology and Parasitology” in academic year 2016./2017.

The practicals are held in Practical room of the Department of Medical Microbiology and Parasitology on the 1st floor of PAC (Pathology-Anatomy Complex).

Thursday, Lecture B1 (4h): Prof. Marija Tonkić, MD. PhD. Practical B1(4h): Introduction to microbiological laboratory and 10.11. 2016. Introduction to medical microbiology. Bacterial structure, the basics of safe laboratory work. Laboratory-acquired infections. 8,00 – 11,15 physiology and genetics. Pathogenesis of bacterial diseases. Microscopic examination of principal bacterial shapes. Differential Amfiteatar, PAC Bacterial antigens and vaccines. staining in bacteriology. Cultivation of bacteria. Media types. Group 1: 11,20 – 14,35 Dr. Žana Rubić, MD. Group 2: 14,40 – 17,55 A/Prof. Vanja Kaliterna, MD. PhD.

Friday, Lecture B2 (2h): A/Prof. Ivana Goić Barišić, MD. PhD. Practical B1(4h): Introduction to microbiological laboratory and 11. 11. 2016. Antibacterial agents. Antimicrobial resistance in bacteria. the basics of safe laboratory work. Laboratory-acquired infections. 8,00 – 9,35 Microscopic examination of principal bacterial shapes. Differential Amfiteatar, PAC staining in bacteriology. Cultivation of bacteria. Media types. Group 3: 9,40 – 12,55 A/Prof. Vanja Kaliterna, MD. PhD. Group 4: 13,00 – 16,15 Dr. Žana Rubić, MD.

1 Monday, Seminar B1 (2h): A/Prof. Ivana Goić Barišić, MD. PhD. Practical B2 (4h): Performing, reading and interpretation of the 14. 11. 2016. Genera Streptococcus, Staphylococcus, Enterococcus, Neisseria, antibiogram (disk diffusion method, broth dilution, agar dilution, Haemophilus E-test). Collecting and inoculating of throat swab. Principles of Classroom, PAC 8,00 – 9,35 Seminar group II isolation and identification of pyogenic cocci. 9,40 – 11,15 Seminar group I Group 4: 9,40 – 12,55 A/Prof. Vanja Kaliterna, MD. PhD. Group 3: 13,00 – 16,15 Dr.Merica Carev, MD. Tuesday, Seminar B2 (2h): Dr. Anita Novak, MD. Practical B2 (4h): Performing, reading and interpreting the 15.11. 2016. Characteristics of bacteria from the family Enterobacteriaceae. antibiogram (disk diffusion method, broth dilution, agar dilution, Genera Bordetella, Brucella. E-test). Collecting and inoculating throat swab. Principles of Microscop., PAC 8,00 – 9,35 Seminar group I isolation and identification of pyogenic cocci. 9,40 – 11,15 Seminar group II Group 2: 9,40 – 12,55 Dr.Merica Carev, MD. Group 1: 13,00 – 16,15 A/Prof. Vanja Kaliterna, MD. PhD. Wednesday, Seminar B3 (2h): A/Prof. Ivana Goić Barišić, MD. PhD. Practical B3 (4h): Macroscopic and biochemical identification 16.11. 2016. Gram-negative nonfermenting bacteria – genera Pseudomonas, of Enterobacteriaceae. Serotyping. Serological testing (Widal, Acinetobacter. Gram-negative, curved, rod-shaped bacteria – agglutination of Yersiniae). Characteristics of genera Bordetella, genera Vibrio, Helicobacter, Campylobacter. Anaerobic bacteria Brucella. ASO test. – genera Clostridium, Lactobacillus, Actinomyces; family – Group 1: 9,40 – 12,55 Dr. Anita Novak, MD. PhD. Microscop., PAC Bacteroideaceae Group 2: 13,00 – 16,15 Dr. Žana Rubić, MD. 8,00 – 9,35 Seminar group I Thursday, Seminar B3 (2h): A/Prof. Ivana Goić Barišić, MD. PhD. Practical B3 (4h): Macroscopic and biochemical … 17.11. 2016. Gram-negative nonfermenting bacteria – genera … Group 3: 9,40 – 12,55 Dr. Žana Rubić, MD. Microscop., PAC 8,00 – 9,35 Seminar group II Group 4: 13,00 – 16,15 Dr. Anita Novak, MD. PhD.

Friday, Lecture B3 (3h): Prof. Marija Tonkić, MD. PhD. Practical B4 (4h): Pseudomonas on OA, resistant strains. 18. 11. 2016. Gram-negative spiral bacteria – family Spirochaetaceae. Campylobacter - cultivation and microscopy. Vibrio – culture. 8,00 – 10,25 Cell wall-defective bacteria – family Mycoplasmataceae. Obligate Helicobacter in tissue sample. Collecting and transporting of Amfiteatar, PAC intracellular bacteria: Rickettsiaceae, Chlamydiaceae specimens for cultivation of anaerobic bacteria. Principles of anaerobic cultivation. Neisseria spp., Haemophilus. Group 2: 11,20 – 14,35 Dr. Žana Rubić, MD. Group 1: 14,40 – 17,55 A/Prof. Vanja Kaliterna, MD. PhD.

2 Monday, Seminar B4 (2h): A/Prof. Ivana Goić Barišić, MD. PhD. Practical B4 (4h): Pseudomonas on OA, resistant strains… 21. 11. 2016. Genera – Bacillus, Corynebacterium, Listeria, Legionella. Acido- Group 3: 9,40 – 12,55 A/Prof. Vanja Kaliterna, MD. PhD. resistant bacteria – genus Mycobacterium. Group 4: 13,00 – 16,15 Dr. Žana Rubić, MD. Microscop., PAC 8,00 – 9,35 Seminar group II 9,40 – 11,15 Seminar group I Tuesday, Practical B5 (4h): B. anthracis – presentation of spores and 22.11. 2016. capsule, B. subtillis – spores cultivation. Corynebacterium – cultivation, staining and microscopy. Listeria – culture, microscopy. Acid-fast staining (Ziehl-Nielsen). Specimens collecting, transporting and processing for isolation of mycobacteria. Cultivation of mycobacteria. Test of resistance to tuberculostatics. Serologic tests for T. pallidum. Mycoplasma. Ureaplasma – culture. Group 1: 8,00 – 11,00 Dr. Žana Rubić, MD. Group 2: 11,00 – 14,00 Dr. Anita Novak, MD. PhD. Group 4: 14,00 – 17,00 Dr. Žana Rubić, MD. Group 3: 17,00 – 20,00 A/Prof. Vanja Kaliterna, MD. PhD. Wednesday, Repeating 23. 11. 2016. Thursday, KNOWLEDGE TEST (Bacteriology) - exam 24. 11. 2016. 11,20 –12,55 PAC(Microscop.,,Classroom,Comp.r.) Friday, Lecture M (2h): A/Prof. Ivana Goić Barišić, MD. PhD. 25. 11. 2016. Introduction to medical mycology. Morphology and reproduction 9,40 – 11,15 of the fungi. Fungal diseases - pathogenesis. Antifungal agents. BR,Firule Seminar M (2h): Medically important fungi. Microscop., PAC 11,20–12,55 Seminar group I Dr. Anita Novak, MD. PhD. Classroom, PAC 11,20–12,55 Seminar group II Dr. K. Šiško Kraljević, MD. PhD.

3 Monday, Practical M (2h): Yeasts and moulds – macro and 28. 11. 2016. micromorphology. Principles of isolation and identification od medically important yeasts and moulds. Serological diagnosis of mycoses. Diagnosis of dermatomycosis. Group 4: 8,00 – 9,35 Dr.Merica Carev, MD. Group 3: 9,40 – 11,15 Dr.Merica Carev, MD. Group 2: 11,20 – 12,55 Dr. Žana Rubić, MD. Group 1: 13,00 – 14,35 Dr. Žana Rubić, MD. Tuesday, Lecture P (3h): Prof. Marija Tonkić, MD. PhD. 29. 11. 2016. Introduction to medical parasitology. Medical protozoology. 8,00 – 10,25 Blood and tissue protozoa - genera: Toxoplasma, Plasmodium, Amfiteatar,PAC Leishmania.

Seminar P1 (2h): Protists of gastrointestinal and urogenital Microscop., PAC system – genera: Giardia, Entamoeba, Cryptosporidium, Classroom, PAC Trichomonas. 11,20 – 12,55 Seminar group I Dr. Anita Novak, MD. PhD. 11,20 – 12,55 Seminar group II Dr. K. Šiško Kraljević,MD.PhD. Wednesday, Seminar P2 (2h): Dr. Anita Novak, MD. PhD. Practical P1 (4h): Diagnosis of toxoplasmosis, leishmaniasis and 30. 11. 2016. Nematodes and Cestodes: Trichinella, Trichuris, Strongyloides, malaria. Giemsa-Romanowsky-stained biopsy specimen, thick and Enterobius, Ascaris, Taenia, Echinococcus. thin blood films. Preparing, staining and microscopy of specimen Microscop., PAC 9,40 – 11,20 Seminar group II (recognition of parasitic forms). Serological diagnostic testing. Group 1: 8,00 – 11,15 Dr. Žana Rubić, MD. Group 2: 11,20 – 14,35 Dr.Merica Carev, MD.

Thursday, Seminar P2 (2h): Dr. Katarina Šiško Kraljević, MD. PhD. Practical P1 (4h): Diagnosis of toxoplasmosis, leishmaniasis and 1. 12. 2016. Nematodes and Cestodes: Trichinella, Trichuris, Strongyloides, malaria. Giemsa-Romanowsky-stained biopsy specimen, thick and Enterobius, Ascaris, Taenia, Echinococcus. thin blood films. Preparing, staining and microscopy of specimen Microscop., PAC 8,00 – 9,35 Seminar group I (recognition of parasitic forms). Serological diagnostic testing. Group 3: 8,00 – 11,15 Dr.Merica Carev, MD. Group 4: 11,20 – 14,35 Dr. Žana Rubić, MD.

4 Friday, Practical P2 (4h): Diagnosis of intestinal parasitosis. Microscopy 2. 12. 2016. of direct wet mount, Lugol's iodine and concentration method (MIFC). Micromorphology of protozoan cysts, eggs and larval helmints. Recognition of adult forms. Graham's anal swab. Diagnosis of echinococcosis and trichinellosis. Group 2: 8,00 – 11,15 Dr. Vanja Kaliterna, MD. Group 1: 11,20 – 14,35 Dr. Žana Rubić, MD.

Monday, Practical P2 (4h): Diagnosis of intestinal parasitosis. Microscopy 5. 12. 2016. of direct wet mount, Lugol's iodine and concentration method ... Group 4: 8,00 – 11,15 Dr. Žana Rubić, MD. Group 3: 11,20 – 14,35 Dr. Vanja Kaliterna, MD. Tuesday, Repeating 6. 12. 2016. Wednesday, KNOWLEDGE TESTING (Mycology+Parasitology) - exam 7. 12. 2016. 11,20 – 12,05 PAC(Amfiteatar, Microsc., Computer room)

Thursday, Lecture V1 (3h):. Dr. Irena Tabain, MD. PhD. Practical V1 (3h): Clinical samples in virology. Cultivation of 8. 12. 2016. Introduction to virology. Chemical composition and structure of viruses in laboratory animals. 8,00-10,25 viruses. Viral antigens and hemagglutination. Replication of Cultivation of viruses in chicken embryo - inoculation. Cultivation Amfiteatar, PAC viruses. Pathogenesis of viral diseases. Viral interference and of viruses in cell culture. Microscopy of cythopatic effect. interferon. Chemoprophylaxis and therapy of viral diseases. Viral Microscopy of intracellular inclusions. vaccines. Prions. Group 1: 10,30 – 12,55 Dr. Irena Tabain, MD. PhD.

Friday, Seminar V1 (2h) DNA viruses: Parvoviridae, Papovaviridae, Practical V1 (3h): Clinical samples in virology. Cultivation of 9. 12. 2016. Adenoviridae, Poxviridae viruses in laboratory animals. 8,00-9,35 Seminar group I Dr. Irena Tabain, MD. PhD. Cultivation of viruses in chicken embryo - inoculation. Cultivation Classroom, PAC 9,40-11,15 Seminar group II Dr. Irena Tabain, MD. PhD. of viruses in cell culture. Microscopy of cythopatic effect. Microscopy of intracellular inclusions. Group 3: 11,20 – 13,45 Dr. Irena Tabain, MD. PhD.

5 Monday, Seminar V2 (2h): Herpesviridae. Hepatitis B,C and D Practical V1 (3h): Clinical samples in virology. Cultivation of 12. 12. 2016. 8,00-9,35 Seminar group II Dr. K. Šiško Kraljević, MD. PhD. viruses in laboratory animals. 9,40-11,15 Seminar group I Dr. K. Šiško Kraljević, MD. PhD. Cultivation of viruses in chicken embryo - inoculation. Cultivation Classroom, PAC of viruses in cell culture. Microscopy of cythopatic effect. Microscopy of intracellular inclusions. Group 2: 11,20 – 13,45 Dr. Irena Tabain, MD. PhD. Group 4: 13,50 – 16,15 Dr. Irena Tabain, MD. PhD.

Tuesday, Lecture V2 (2h): Dr. Irena Tabain, MD. PhD. Practical V2 (4h): Serological methods in diagnosing viral 13. 12.2016. Flaviviridae, Togaviridae, Bunyaviridae, Filoviridae, Arenaviridae. diseases. Rapid tests and methods of molecular biology in 8,00-9,35 virology. Amfiteatar, PAC Group 4: 9,40 – 12,55 Dr. Irena Tabain, MD. PhD. Group 2: 13,00 – 16,15 Dr. Irena Tabain, MD. PhD.

Wednesday, Seminar V3 (2h): RNA viruses: Picornaviridae (Enterovirus, Practical V2 (4h): Serological methods in diagnosing viral 14. 12. 2016. Hepatovirus), Caliciviridae. Rhabdoviridae, diseases. Rapid tests and methods of molecular biology in 8,00-9,35 Seminar group II Dr. Irena Tabain, MD. PhD. virology. Classroom, PAC 9,40-11,15 Seminar group I Dr. K. Šiško Kraljević, MD. PhD. Group 1: 11,20 – 14,35 Dr. Irena Tabain, MD. PhD.

Thursday, Seminar V4 (2h): Orthomyxoviridae, Paramyxoviridae, Practical V2 (4h): Serological methods in diagnosing viral 15. 12.2016. Coronaviridae. diseases. Rapid tests and methods of molecular biology in Classroom, PAC 8,00-9,35 Seminar group I Dr. Irena Tabain, MD. PhD. virology. 9,40-11,15 Seminar group II Dr. Irena Tabain, MD. PhD. Group 3: 11,20 – 14,35 Dr. Irena Tabain, MD. PhD.

Friday, Seminar V5 (2h): Reoviridae, Retroviridae. 16.12.2016. 8,00-9,35 Seminar group II Dr. K. Šiško Kraljević, MD. PhD. 9,40-11,15 Seminar group I Dr. K. Šiško Kraljević, MD. PhD.

6 Monday, KNOWLEDGE TESTING (Virology) - exam 19. 12. 2016. 11,20 – 12,05 PAC(Microscop. ,Classroom, Seminar room 2)

Tuesday, KNOWLEDGE TESTING other partial exams 20. 12.2016. 9,40-11,15 PAC(Clasroom)

Wednesday, Practical/ Oral exam 21.12.2016.

Thursday, Practical/ Oral exam 22.12.2016.

EXAMS 1. 21.-22. 12. 2016. 2. 21.07. 2017. 3. 30.08. 2017. 4. 13. 09. 2017. Head of the Department: Prof. Marija Tonkić, MD. PhD