The Wonderful World of the Microscope

The Wonderful World of the Microscope Anton van Leeuwenhoek - Dutch Businessman 1600’s - Invented first powerful microscope (300X) - Examined pond water & saw “little animals”

TYPES OF MICROSCOPES 1. Simple Microscope- 1 lens. Ex. Magnifying lens 2. Compound Light Microscope- uses light and many lenses to view small objects. *Our Lab* 3. Electron Microscope- uses a beam of electrons to view extremely small objects.

Magnification- Enlargement of a small object. Ocular Lens X Objective Lens = Total Magnification 10X X 4X = 40X 10X X 10X = 100X 10X X 40X = 400X

Proper Carrying Procedure One hand on arm, one hand on base, electric cord securely wrapped and looking forward.

Proper Microscope Technique 1. Put slide under stage clips. 2. Focus using low power (4X). Coarse -> Fine 3. Switch to higher power (10X or 40X). *ONLY USE FINE ADJUSTMENT ON 40X LENS! Parts of a Compound Light Microscope Eyepiece- piece you look through, magnifies 10 times (10x)

Arm- supports body tube

Body Tube- tube of mirrors connecting objective lens and eyepiece

Stage- holds the slide

Stage Clips- holds slides in place

Stage Opening- circle where light passes through

Coarse Adjustment- used for FIRST focusing, moves body tube

Fine Adjustment- sharpens image, final focusing

Base- bottom

Illuminator/Light-

Diaphragm- controls the amount of light

Low Power Objective- 4x or 10x; locates specimen

High Power Objective- 40x; greater magnification

Revolving Nosepiece- moves lens into place

Slide- holds the specimen (glass or plastic)

Slide Cover- covers specimen so it doesn’t move

Lens Paper- cleans lenses