Resume: A.Raskin Anna Jokiel Raskin [email protected] (858) 692-4100 (858) 964-2284

Summary: I have worked as a research engineering PhD intern for the R&D team at Abbott Vascular involved in the development of regional drug delivery devices for the treatment of cardiovascular disease. I also have experience working in the manufacturing department at ISIS, optimizing the synthesis of oligonucleotides. As a PhD student I have applied a few engineering concepts to answer questions pertaining to the development of hypertrophy and heart failure.

Education: 2003 – Present University of California San Diego San Diego, CA PhD Student of Bioengineering: Cardiac Mechanics Research Group

. GPA 4.0 . Designed (Auto-CAD), developed, optimized, and validated mouse cardiac tissue culture chamber (6-12 hour culture) that facilitates uni-axial straining while simultaneously measuring muscle force and strain. . System is capable of inducing hypertrophy at the level of molecular biology in intact isolated cardiac tissue specimens obtained from genetically engineered mice used as models for human heart disease. . Developed experimental techniques which study how systolic and diastolic stress and strain patterns influence load induced hypertrophic gene and protein expression, cardiac remodeling, and development of heart failure. . Real time PCR techniques as well as two photon absorption techniques (confocal microscopy) used to quantify changes in gene expression, cellular signaling, and to analyze load induced cyto- architectural structural changes. . Fast Fourier analysis and cross correlation techniques implemented (Matlab) to analyze periodicity and deformation of structural proteins within cardiac tissue. . Managed two undergraduate students, who assisted with immunostaining and CAD design. . FDA regulatory class: Class 1,2,3 medical devices, IDE, 510(k), PMA, post market controls

Skills: Q-PCR, western blot, high resolution gel electrophoresis, silver staining of gels, Matlab, Visual Basic (VB), AutoCAD, immunohistochemistry, confocal microscopy, student of national center of microscopy and imaging resources, small animal microsurgery, mouse tissue culture, fluent in Polish, have studied Spanish

Graduate Student Instructor: . Graduate Biomedical Imaging Course: signal processing, image processing, sampling theory, linear systems theory, windowing, modulation, convolution, and FFT theory etc. . Mass and Heat Transfer Course: Diffusion and Convection driven processes . Bioengineering lab course: stress/strain analysis of living tissue, second order system analysis, skeletal muscle mechanics and physiology, cardiac muscle mechanics and physiology, etc. . Biomaterials Science Course: Properties of materials, polymers, hydrogels, bioresorbable and bioerodible materials, composite mechanics, fabrication, material fatigue, friction, wear, and failure

2000 – 2003 University of California San Diego San Diego, CA B.S. in Bioengineering (ABET)

. GPA 3.82 . Graduated Magna Cum Laude . Senior Project: Development of red blood cell velocity program (Matlab) based on the theory of times series cross correlation (hemodynamics research group, UCSD)

Experience: Summer 2007 Abbot Vascular (CV Medical Device) Santa Clara, California

. With minimal direction I executed research which studied the effect of drug solubility on the drug release mechanisms from drug delivery vehicles used for the treatment of cardiovascular disease . Experience with making biodegradable nanoparticle drug delivery systems Resume: A.Raskin . Came up with relevant questions . Designed in vivo and in vitro experiments to answer these questions . Summarized, analyzed, and came up with conclusions from experiments. Results and future direction of project presented to executive V.P. Abbott Vascular team. . Utilized SEM (electron microscopy), DSC, GPC, DLS analysis to characterize drug delivery vehicles

2001 – 2003 Isis Pharmaceuticals (Antisense Technology) Carlsbad, California

. Under the direction of a manager I optimized oligonucleotide synthesis and analyzed drug purity (HPLC) . Analytical model derivations for the analysis of drug purity . Assisted liaison (Lilly/ Isis) with data analysis and company presentations . Developed VB program capable of attaining from Access micro-array gene expression data in order to present data in heat map form . Worked on BIRD knowledge base Publications: . Sheikh F., Raskin A., Omens J., McCulloch A., Chen J.: A novel stretch effector within sarcomeric I band is critical for the transition to the pathological cardiac hypertrophy, JCI (In review) . Wang J, Hoshijima M, Lam J, Zhou Z, Jokiel A, Dalton ND, Hultenby K, Ruiz-Lozano P, Ross J Jr, Tryggvason K, Chien KR: Cardiomyopathy associated with microcirculation dysfunction in laminin alpha4 chain-deficient mice, J Biological Chem 2006 Jan 6;281(1):213-20 . Raskin A., Sheikh F., Lee L., Scott B., Belke D., A McCulloch, Chen J., Omens J.: Mechanical Role of FHL1 in Modulating Titin Based Passive Myocardial Tension, (In Progress to Publication) . Raskin A., Swanson E., Hoshijima M., Graves A., Omens J., McCulloch A.: Hypertrophic Gene Expression Induced by Chronic Stretch of Excised Mouse Heart Muscle, (MCB, In Press) Conferences: . Effectively communicated research results and conclusions at national and international conferences . Established collaborations with labs from the US, Canada, and Germany. Collaborations aided in experimental design and scientific discovery. . Invited lecturer at the University of Arizona, April 2008 . Abstracts:  Jokiel A., Omens J., McCulloch A.: Chronic Function in excised Heart Muscle : Role of Laminin-α4 in Cardiac Hypertrophy, Engineering Research Expo, University of California, San Diego, March 2004  Raskin A., Hoshijima M., McCulloch A., Omens J.: The cardiac tissue system: a novel technique capable of inducing hypertrophy in excised heart muscle, Biophysics, Salt Lake City, Utah, March 2006  Raskin A., Sheikh F., Omens J., McCulloch A., Chen J.: Mechanisms of mechanotransduction in FHL1 deficient cardiac muscle, Biophysics, Baltimore, Maryland, March 2007  Raskin A., Sheikh F, Hoshijima M., McCulloch A., Omens J.: Mechanical role of FHL1 in modulating titin based passive tension, Biophysics, Long Beach, California, February 2008  Raskin A., Sheikh F., Omens J., McCulloch A.: Changes in diastolic stress, not systolic stress, attenuate load induced hypertrophy in FHL1 null myocardium, FASEB, San Diego, California, April 2008 Leadership and Volunteer Activities: . Active member of Graduate Bioengineering (BE) Women’s organization at UCSD  Organized, found sponsors for, invited lecturers, and lead Graduate BE Women’s breakfast (October 2007) o Invited lecturer: Dr. Rina Schul . Volunteer of ‘Help to Poland’  Collected and accounted donations for poor children in Russia and Poland Other activities: . Long distance runner: San Diego Track Club, West Coast Runners Club Resume: A.Raskin