1St BASE Custom Pab Production Order Form

Axil Scientific Pte Ltd First BASE Laboratories Sdn Bhd (604944-X) Antibody Production 41 Science Park Road, #01-22/23 The Gemini Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, Seksyen 2, PAb 8-week Project Information Singapore Science Park II, Singapore 117610 43300 Seri Kembangan, Selangor, Malaysia T: +65 6775 7318 T: +603 8943 3252 F: +65 6775 7211 F: +603 8943 3243 E: [email protected] E: [email protected]

Name of Researcher: Email: Address: Contact No. Purchase Order Number: Project Name:

Please choose preferred package: PAb 8-week Package 1, 2, 3, 4 (see overleaf for details) Immunogen Details: *Immunogen provided by Researcher (for Package 1 only)

Protein / Peptide Size: kDa Amount: mg

Form of Protein: Solution (Concentration: mg/ml) (Buffer contains: ) Lyophilised Gel Strip

* Note: 4 mg of soluble Immunogen required for Affinity Purification and 1 mg for each ELISA Testing (2 animals)

Additional Services: Conjugation of Immunogen to KLH carrier protein Note: 3mg of antigen in 1xPBS required (min. of 1mg/ml)

Peptide Synthesized by 1st BASE (included in Package 2, 3 and 4) Sequence: NH2 - -OH Note: a) FREE up to 15 residues, > 70% purity for Package 2, 3 and 4 Optional purification of peptides: b) Recommended < 30 residues for other Custom Package Purity > 80% by HPLC (charges applied) c) Allow 2 weeks of synthesis Purity > 95% by HPLC (charges applied)

Shipment Information: Use 1st BASE’s Fedex Account Charge to my FedEx Account (No. )

Preference: Ship 2ml Aliquots of Bleeds 1 & 2 (note: additional charges may apply) Ship after Bleed 2 & at end of project (total of 2 shipments) (note: additional charges may apply) Upon Project Completion

Custom PAb Packages and Services

of 2 Please Package Details Remarks Please Additional Details Remarks Tick Tick Services:

PAb 8- Polyclonal Antibody Production - 2 Rabbits *2mg of ELISA Testing ELISA Testing and Reporting, on top of 0.4mg week (*Immunogen provided by researcher) antigen in and Reporting ELISA on Bleed 1 (which already included in soluble Package Includes: 1xPBS (additional) Packages 1 to 4) antigen per 1 -Immunization and anti-sera production (75ml/rabbit) preferred Please indicate which Bleed? 2, 3 ELISA -ELISA Testing for Bleed 1 (min. of 1mg/ml)

PAb 8- Polyclonal Antibody Production - 2 Rabbits Protein A / G Protein A/G Purification (25ml of purified week Includes: Purification anti-sera). Package -Peptide synthesis (15aa), Purification (> 70% Purity) (25ml) Please state which bleed? 1, 2, 3 2 and Conjugation -Immunization and anti-sera production (75ml/rabbit) -ELISA Testing for Bleed 1

PAb 8- Polyclonal Antibody Production - 2 Rabbits Affinity Affinity Column Preparation and week Includes: Purification Purification of Bleeds (25ml of purified Package -Peptide synthesis (15aa), Purification (> 70% Purity) (25ml) anti-sera) . Contact us Min. 3mg 3 and Conjugation Please state which bleed? 1, 2, 3 of soluble -Immunization and anti-sera production (62.5mL/rabbit) for a free peptide antigen -Affinity Purification on Bleed 3 for 12.5mL/ rabbit required (includes reusable column) antigenicity -ELISA Testing on Bleed 1 analysis! PAb 8- Polyclonal Antibody Production - 2 Rabbits Large scale Large scale Affinity Column Preparation week Includes: Affinity and Purification (150-200ml of purified Package -Peptide synthesis, Purification (> 70% Purity) Purification serum) 4 and Conjugation - Immunization and anti-sera production - Large scale Affinity Purification for all Bleeds (75mL/ rabbit) - ELISA Testing on Bleed 1 Rabbit Exsanguination of Animals for Project Exsanguination Termination (Terminal bleed will be about 60ml per animal) Project Project Extension (28 days) includes Extension -1 Additional Boost and -2 Bleeds (25ml each) for 2 rabbits Custom Screening of PAb serum on a choice of Antibody IHC tissue panel. Screening-IHC Custom Screening of PAb serum on choice of Antibody 14 Western Blot lysates Screening-WB