Supplementary information
Characterisation of Klebsiella sp. S1: a bacterial producer of secoisolariciresinol through biotransformation
Yu-Jie Zhou1,2, Songling Zhu1,2, Dong-Hui Yang3, Dan-Dan Zhao1,2, Jia-Jing Li1,2, Shu-Lin Liu1,2,4* 1Systemomics Center, College of Pharmacy, and Genomics Research Center (State- Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, China. 2HMU-UCFM Centre for Infection and Genomics, Harbin Medical University, Harbin, China 3Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, China 4Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada. *Corresponding author: Shu-Lin Liu E-mail: [email protected] Tel: +8645186692236, Fax: +8645187086735 Table S1. Predicted coding sequences unique to Klebsiella S1 Gene_id {KEGG} Annotation {SwissProt} Klebsiella-S1GL001339 {NA} Klebsiella-S1GL001354 {NA} Klebsiella-S1GL002682 {NA} Klebsiella-S1GL001640 {K09961 K09961 hypothetical protein -- Unclassified; Poorly Characterized; Function unknown} Klebsiella-S1GL004549 {NA} Klebsiella-S1GL001035 {NA} Klebsiella-S1GL003329 {NA} Klebsiella-S1GL001360 {NA} Klebsiella-S1GL003350 {K02371 fabK enoyl-[acyl carrier protein] reductase II 1.3.1.- Metabolism; Lipid Metabolism; Fatty acid biosynthesis [PATH:ko00061] Metabolism; Lipid Metabolism; Lipid biosynthesis proteins [BR:ko01004]} Klebsiella-S1GL004180 {NA} Klebsiella-S1GL003232 {NA} Klebsiella-S1GL003230 {K01104 E3.1.3.48 protein-tyrosine phosphatase 3.1.3.48 Unclassified; Cellular Processes and Signaling; Signal transduction mechanisms} Klebsiella-S1GL001658 {NA} Klebsiella-S1GL003785 {NA} Klebsiella-S1GL000774 {NA} Klebsiella-S1GL001649 {NA} Klebsiella-S1GL001355 {NA} Klebsiella-S1GL001353 {NA} Klebsiella-S1GL000592 {NA} Klebsiella-S1GL004550 {K00064 E1.1.1.122 D-threo-aldose 1-dehydrogenase 1.1.1.122 Metabolism; Carbohydrate Metabolism; Ascorbate and aldarate metabolism [PATH:ko00053]} Klebsiella-S1GL001628 {NA} Klebsiella-S1GL003596 {NA} Klebsiella-S1GL002678 {NA} Klebsiella-S1GL002575 {K06885 K06885 -- -- Unclassified; Poorly Characterized; General function prediction only} Klebsiella-S1GL001346 {NA} Klebsiella-S1GL004548 {NA} Klebsiella-S1GL000777 {NA} Klebsiella-S1GL004129 {K09690 ABC-2.LPSE.P lipopolysaccharide transport system permease protein -- Environmental Information Processing; Membrane Transport; Transporters [BR:ko02000] Environmental Information Processing; Membrane Transport; ABC transporters [PATH:ko02010]} Klebsiella-S1GL002749 {NA} Klebsiella-S1GL004373 {NA} Klebsiella-S1GL004175 {NA} Klebsiella-S1GL002679 {NA} Klebsiella-S1GL001648 {NA} Klebsiella-S1GL001659 {NA} Klebsiella-S1GL003597 {K07741 K07741 anti-repressor protein -- Unclassified; Genetic Information Processing; Transcription related proteins} Klebsiella-S1GL001343 {NA} Klebsiella-S1GL003231 {K00903 E2.7.10.- protein-tyrosine kinase 2.7.10.- Unclassified; Cellular Processes and Signaling; Signal transduction mechanisms} Klebsiella-S1GL003233 {NA} Klebsiella-S1GL001042 {NA} Klebsiella-S1GL002576 {NA} Klebsiella-S1GL002596 {NA} Klebsiella-S1GL001347 {NA} Klebsiella-S1GL003157 {NA} Klebsiella-S1GL004176 {NA} Klebsiella-S1GL002691 {NA} Klebsiella-S1GL002722 {K06919 K06919 putative DNA primase/helicase -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL001655 {NA} Klebsiella-S1GL003782 {NA} Klebsiella-S1GL002689 {NA} Klebsiella-S1GL003380 {NA} Klebsiella-S1GL004768 {K11911 vasL type VI secretion system protein VasL -- Environmental Information Processing; Membrane Transport; Secretion system [BR:ko02044]} Klebsiella-S1GL003352 {NA} Klebsiella-S1GL004368 {NA} Klebsiella-S1GL002680 {NA} Klebsiella-S1GL000601 {NA} Klebsiella-S1GL003602 {NA} Klebsiella-S1GL001344 {NA} Klebsiella-S1GL000593 {K07493 K07493 putative transposase -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL003234 {NA} Klebsiella-S1GL002595 {NA} Klebsiella-S1GL000776 {NA} Klebsiella-S1GL002671 {NA} Klebsiella-S1GL001361 {NA} Klebsiella-S1GL001041 {NA} Klebsiella-S1GL003351 {NA} Klebsiella-S1GL002747 {NA} Klebsiella-S1GL002688 {NA} Klebsiella-S1GL003328 {K01506 E3.5.-.- -- -- Unclassified; Metabolism; Others} Klebsiella-S1GL004127 {NA} Klebsiella-S1GL001037 {NA} Klebsiella-S1GL003783 {NA} Klebsiella-S1GL004181 {NA} Klebsiella-S1GL003606 {K06919 K06919 putative DNA primase/helicase -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL002690 {NA} Klebsiella-S1GL003603 {NA} Klebsiella-S1GL001345 {NA} Klebsiella-S1GL000600 {NA} Klebsiella-S1GL003595 {NA} Klebsiella-S1GL002712 {NA} Klebsiella-S1GL002748 {K06919 K06919 putative DNA primase/helicase -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL001356 {NA} Klebsiella-S1GL003235 {NA} Klebsiella-S1GL004376 {NA} Klebsiella-S1GL001670 {NA} Klebsiella-S1GL000771 {K07489 K07489 transposase -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL004374 {NA} Klebsiella-S1GL002674 {NA} Klebsiella-S1GL003330 {NA} Klebsiella-S1GL004130 {K09691 ABC-2.LPSE.A lipopolysaccharide transport system ATP-binding protein -- Environmental Information Processing; Membrane Transport; Transporters [BR:ko02000] Environmental Information Processing; Membrane Transport; ABC transporters [PATH:ko02010]} Klebsiella-S1GL003604 {NA} Klebsiella-S1GL003609 {NA} Klebsiella-S1GL001340 {NA} Klebsiella-S1GL002746 {NA} Klebsiella-S1GL002711 {NA} Klebsiella-S1GL002693 {NA} Klebsiella-S1GL004183 {NA} Klebsiella-S1GL000770 {NA} Klebsiella-S1GL003332 {NA} Klebsiella-S1GL000599 {NA} Klebsiella-S1GL001350 {NA} Klebsiella-S1GL001654 {NA} Klebsiella-S1GL003349 {NA} Klebsiella-S1GL002681 {NA} Klebsiella-S1GL002536 {NA} Klebsiella-S1GL004178 {NA} Klebsiella-S1GL000594 {NA} Klebsiella-S1GL002692 {NA} Klebsiella-S1GL002745 {NA} Klebsiella-S1GL004553 {NA} Klebsiella-S1GL001645 {NA} Klebsiella-S1GL001669 {NA} Klebsiella-S1GL002736 {NA} Klebsiella-S1GL000598 {NA} Klebsiella-S1GL003327 {NA} Klebsiella-S1GL001622 {NA} Klebsiella-S1GL003220 {K01247 alkA DNA-3-methyladenine glycosylase II 3.2.2.21 Genetic Information Processing; Replication and Repair; Base excision repair [PATH:ko03410] Genetic Information Processing; Replication and Repair; DNA repair and recombination proteins [BR:ko03400]} Klebsiella-S1GL004525 {NA} Klebsiella-S1GL000773 {NA} Klebsiella-S1GL003599 {NA} Klebsiella-S1GL001342 {NA} Klebsiella-S1GL001351 {NA} Klebsiella-S1GL004172 {K07733 alpA prophage regulatory protein -- Genetic Information Processing; Transcription; Transcription factors [BR:ko03000]} Klebsiella-S1GL003598 {NA} Klebsiella-S1GL001349 {NA} Klebsiella-S1GL003848 {K00883 E2.7.1.58, dgoK 2-dehydro-3- deoxygalactonokinase 2.7.1.58 Metabolism; Carbohydrate Metabolism; Galactose metabolism [PATH:ko00052]} Klebsiella-S1GL001045 {NA} Klebsiella-S1GL004132 {NA} Klebsiella-S1GL001033 {NA} Klebsiella-S1GL002709 {NA} Klebsiella-S1GL004375 {K03658 helD DNA helicase IV 3.6.4.12 Genetic Information Processing; Replication and Repair; DNA repair and recombination proteins [BR:ko03400]} Klebsiella-S1GL000597 {NA} Klebsiella-S1GL003331 {K00540 E1.-.-.- -- -- Unclassified; Metabolism; Others} Klebsiella-S1GL004552 {NA} Klebsiella-S1GL003354 {NA} Klebsiella-S1GL004524 {NA} Klebsiella-S1GL000772 {K07480 insB insertion element IS1 protein InsB -- Unclassified; Genetic Information Processing; Replication, recombination and repair proteins} Klebsiella-S1GL003236 {K00754 E2.4.1.- -- -- Metabolism; Carbohydrate Metabolism; Fructose and mannose metabolism [PATH:ko00051]} Klebsiella-S1GL001348 {NA} Klebsiella-S1GL002669 {NA} Klebsiella-S1GL004551 {K00100 E1.1.1.- -- -- Metabolism; Carbohydrate Metabolism; Fructose and mannose metabolism [PATH:ko00051] Metabolism; Carbohydrate Metabolism; Butanoate metabolism [PATH:ko00650] Metabolism; Lipid Metabolism; Linoleic acid metabolism [PATH:ko00591] Metabolism; Xenobiotics Biodegradation and Metabolism; Chloroalkane and chloroalkene degradation [PATH:ko00625] Metabolism; Xenobiotics Biodegradation and Metabolism; Bisphenol degradation [PATH:ko00363]} Klebsiella-S1GL001352 {NA} Klebsiella-S1GL003797 {K01993 ABC-2.TX HlyD family secretion protein -- Unclassified; Cellular Processes and Signaling; Membrane and intracellular structural molecules} Klebsiella-S1GL001664 {NA} Klebsiella-S1GL000775 {NA} Klebsiella-S1GL001034 {K01153 hsdR type I restriction enzyme, R subunit 3.1.21.3 Unclassified; Genetic Information Processing; Restriction enzyme} Klebsiella-S1GL002725 {NA} Klebsiella-S1GL003379 {NA} Klebsiella-S1GL002684 {NA} Klebsiella-S1GL001341 {NA} Klebsiella-S1GL001046 {NA} Klebsiella-S1GL000662 {NA} Klebsiella-S1GL004131 {NA}