The Role of Environmental Factors in the Induction of Oxidative Stress in Zebra Mussel

AQUATIC ECOLOGY

The role of environmental factors in the induction of oxidative stress in zebra mussel (Dreissena polymorpha)

Adrianna Wojtal-Frankiewicz1*, Joanna Bernasińska2, Piotr Frankiewicz1, Krzysztof Gwoździński2 & Tomasz Jurczak1

1Department of Applied Ecology, University of Lodz 90-237 Lodz, Banacha 12/16 Str, Poland *Corresponding author: adwoj @ biol.uni.lodz.pl tel. (48) 042-635-43-30 ; fax. (48) 042-665-58-19

2Department of Molecular Biophysics, University of Lodz 90-236 Lodz, Pomorska 141/143 Str, Poland

Online resources 4. Physical parameters and ion concentrations in the Sulejow Reservoir and Lake Białe within the studied seasons. 1 Spring Summer Late Summer Autumn Sulejow Białe Sulejow Białe Sulejow Białe Sulejow Białe 2010 Physical parameters Temperature (oC) 17.60 17.2 - - 24.50 22.70 11.50 11.50 Oxygen con. (mg L-1) 5.67 6.83 - - 9.32 7.50 6.86 9.27 pH 7.48 7.45 - - 8.86 8.30 7.72 7.46 Conductivity (S cm-1) 276 281 - - 300 318 357 336

Ions (mg L-1) phosphate 0.040 0.004 - - 0.003 0.004 0.039 0.005 nitrate 2.37 0.49 - - 0.28 0.04 4.14 0.05 chloride 8.24 17.22 - - 12.89 16.44 13.34 16.87 sodium 3.66 11.18 - - 7.91 10.76 8.11 11.17 potassium 6.29 32.29 - - 10.44 32.14 9.79 32.05 sulfates 27.40 45.48 - - 26.76 44.39 22.97 44.62 magnesium 1.12 2.62 - - 1.32 1.98 1.29 1.78 calcium 48.80 36.82 - - 50.07 36.79 52.68 41.47

2011 Physical parameters Temperature (oC) 21.60 23.00 21.7 22.50 22.50 21.60 18.20 18.80 Oxygen con. (mg L-1) 9.04 10.3 9.55 11.35 11.56 8.41 10.84 8.98 pH 8.31 8.94 9.34 9.15 9.99 9.4 9.25 8.87 Conductivity (S cm-1) 344 295 308 309 298 306 298 313

Ions (mg L-1) phosphate 0.05 0.03 0.03 0.02 0.01 0.08 0.01 0.04 nitrate 0.50 0.04 0.79 0.05 0.48 0.05 0.08 0.11 chloride 14.93 18.84 15.33 19.30 15.90 18.94 15.28 19.92 sodium 8.83 11.90 8.69 12.18 9.85 12.05 8.88 12.73 potassium 8.51 23.44 8.94 23.75 8.65 23.73 9.13 24.21 sulfates 30.65 45.99 28.00 46.27 26.69 45.90 25.06 47.57 magnesium 0. 96 1.88 1.57 1.79 1.14 1.87 1.14 2.20 calcium 48.84 39.19 43.13 33.33 51.49 40.29 44.31 42.55

2012 Physical parameters Temperature (oC) 17.20 18.70 25.00 18.50 22.10 20.50 15.40 15.30 Oxygen con. (mg L-1) 17.37 18.64 11.92 9.21 12.30 8.53 11.08 9.52 pH 9.95 9.97 9.00 8.60 8.50 8.00 8.66 8.28 Conductivity (S cm-1) 303 334 329 359 325 345 323 349

Ions (mg L-1) phosphate 0.08 0.03 0.05 0.03 0.15 0.04 0.11 0.05 nitrate 0.21 0.03 0.12 0.04 0.04 0.05 0.01 0.09 chloride 13.16 16.94 11.11 17.14 13.26 17.91 13.51 18.22 sodium 6.59 9.86 6.44 10.15 6.58 10.77 6.24 11.05 potassium 9.55 23.11 17.67 23.09 11.13 23.47 13.28 23.77 sulfates 32.10 45.55 25.39 45.21 26.80 46.44 23.89 46.54 magnesium 0.86 1.74 0.86 1.72 0.87 1.75 1.22 1.79 calcium 63.22 40.63 65.30 41.28 66.45 41.33 38.62 41.99