George Mason University Graduate Council Graduate Course Approval Form s1

George Mason University – Graduate Council Graduate Course Approval Form

All courses numbered 500 or above must be submitted to the Graduate Council for final approval after approval by the sponsoring College, School or Institute.

Graduate Council requires submission of this form for a new course or any change to existing courses. For a new course, please attach a copy of the syllabus and catalog description (with catalog credit format, e.g. 3:2:1). The designated representative of the College, School or Institute should forward the form along with the syllabus and catalog description, if required, as an email attachment (in one file) to the secretary of the Graduate Council. A printed copy of the form with signatures and the attachments should be brought to the Graduate Council meeting. Please complete the Graduate Course Coordinator Form if the proposed changes will affect other units.

Note: Colleges, Schools or Institutes are responsible for submitting new or modified catalog descriptions (35 words or less, using catalog format) to Creative Services by deadlines outlined in the yearly Catalog production calendar.

Please indicate: New___X____ Modify______Delete______

Department/Unit:____MMB______Course Subject/Number:_BIOL 675______

Submitted by:_____P. Royt______Ext:___31058______Email:[email protected]___

Course Title:__Aerosol Biology ______

Effective Term (New/Modified Courses only): _Summer 08__ Final Term (deleted courses only):______

Credit Hours: (Fixed) __4___ (Var.) ______to ______Grade Type (check one)___X__ Regular graduate (A, B, C, etc.) _____ Satisfactory/No Credit only _____ Special graduate (A, B, C, etc. + IP)

Repeat Status*(check one): _X__ NR-Not repeatable ____ RD-Repeatable within degree ____ RT-Repeatable within term *Note: Used only for special topics, independent study, or internships courses Total Number of Hours Allowed: ______

Schedule Type Code(s): 1._LEC_ LEC=Lecture SEM=Seminar STU=Studio INT=Internship IND=Independent Study 2.LAB_ LAB=Lab RCT=Recitation (second code used only for courses with Lab or Rct component)

Prereq _X__ Coreq ___ (Check one):_ Prerequisites: Undergraduate courses in physics, math, and microbiology, and permission of the Director of the Center for Biodefense. __

______Note: Modified courses - review prereq or coreq for necessary changes; Deleted courses - review other courses to correct prereqs that list the deleted course.

Description of Modification (for modified courses):______

Special Instructions (major/college/class code restrictions, if needed):______

Department/Unit Approval Signature:______Date: ______

College/School Committee Approval Signature:______Date:______

Graduate Council Approval Date:______Provost Office Signature:______George Mason University Graduate Course Coordination Form

Approval from other units:

Please list those units outside of your own who may be affected by this new, modified, or deleted course. Each of these units must approve this change prior to its being submitted to the Graduate Council for approval.

Unit: Head of Unit’s Signature: Date:

Unit: Head of Units Signature: Date:

Graduate Council approval: ______Date: ______

Graduate Council representative: ______Date: ______

Provost Office representative: ______Date: ______AEROSOL BIOLOGY SYLLABUS

Catalogue Copy

BIOL 675: Aerosol Biology (4:3:3) Prerequisites: Undergraduate courses in physics, math, and microbiology, and permission of the Director of the Center for Biodefense. This course provides students with a familiarity of state of the art aerosol equipment and techniques used in laboratory-based research pertaining to biological warfare or terrorism threats. Emphasis will be placed on biosafety procedures, techniques, and equipment used in conducting experiments with infectious organisms in a contained environment.

Instructor: Dr. M. Louise M. Pitt and other selected lecturers from USAMRIID Office: Office hours: Office phone: (301) 6194230 Email: [email protected] Web page:

The goal in teaching Aerosol Biology is to teach students the state-of-the-art principles of aerosol sciences with a particular focus on studying aerosol parameters for application in biodefense and infectious disease research. Lecture topics will include basic aerosol physics, particle dynamics, biological aerosols, and current thinking for development of animal models appropriate for the evaluation of medical countermeasures that protect against aerosol challenge. The subject matter covered in this course will provide students with an overall understanding of biological aerosols, the techniques and tools required to systematically generate and deliver biological agents to animals, and to calculate and report delivered dose. The principles will be taught by lectures listed below and will be based both on material provided by the lecturers and contained in the recommended reading materials. Students will also be expected to give a presentation on a topic mutually agreed upon by student and lecturer. Research for this presentation will include research of available scientific literature applicable to the subject.

The goal of the laboratory part of this course is to teach the basic techniques used in a state-of- the-art Aerobiology laboratory. Students will be introduced to the techniques and equipment required to generate, and characterize bioaerosols. Exercises in equipment calibration and set up, aerosol generation, aerosol sampling, respiratory measurements, assays and culture techniques, and dose calculations will provide the necessary information to perform aerosol challenges in a safe and reproducible manner.

The course will be taught within a 2 week period. The content of the course is described below in the list of lectures. Students are expected to attend all lectures and laboratory sessions. Lecture material will be presented by PowerPoint slides, and where appropriate references will be provided for the students. Full student participation is expected, questions or comments to the instructor during the class meeting are encouraged.

Four exams (60% of grade) will be given during the course. These exams will include multiple choice, calculations and written answers. Students will also be required to give a 20 minute presentation (15% of grade) to include PowerPoint slides on a selected topic. The laboratory grade will be 25% of the final grade. The grading policy to be used is described below.

LECTURE SYLLABUS

Lecture Number Topic 1. Introduction to Aerosol Biology 2. Aerosol Generation 3. Aerosol Characterization 4. Aerosol Sampling Exam # 1 5. Dose Definition and Calculation 6. Physiology of the Airways 7. Deposition, Retention, and Clearance of Inhaled Bioaerosols 8. Deployment of Aerosolized Agents, Aerosols Behavior and Infections Exam # 2 9. Aerosol Delivery and Animal Exposure 10. Animal model Development 11. Animal Models for Inhaled Biothreat Agents Exam # 3 12. Biosafety Aspects of Aerosol Inhalation 13. Pharmaceutical Aerosols 14. Communicable Aerosol Infection Exam # 4 15. Emerging Technologies for Aerosol Generation, Delivery and Characterization 16. Student Presentations

------AEROSOL BIOLOGY LABORATORY SYLLABUS

Welcome to the AEROSOL BIOLOGY Laboratory. Our goal is to teach you the basic techniques used in a state-of-the-art Aerobiology laboratory. You will be introduced to the techniques and equipment required to generate, and characterize bioaerosols. Exercises in equipment calibration and set up, aerosol generation, aerosol sampling, respiratory measurements, assays and culture techniques, and dose calculations will provide the necessary information to perform aerosol challenges in a safe and reproducible manner.

Lab # 1 (5 hr) Introduction to aerosol generators and samplers - Calibrating equipment, - SOPs - Documentation Lab # 2 (3.5 hr) Aerosol Systems – chambers and controllers - Assembling systems - Using manual manipulations to balance aerosol system - Sampling - SOPs - Documentation Lab # 3 (5 hr) Quiz - Automated bioaerosol system - Sampling - SOPs - Documentation Lab # 4 (3.5 hr) Quiz - Plethysmography - Animal restraints - SOPs - Documentation Lab # 5 (6 hr) Quiz - Particle size measurements - Aerosol concentration measurements - Humidity control - SOPs - Documentation Lab # 6 (4.5 hr) Quiz - Various generators - Particle size comparison - Aerosol characterization Lab # 7 (5.5 hr) Practical Exercise: Perform an aerosol to include equipment calibration, system set-up, aerosol generation and sample collection, calculation of dose and documentation Total hr: 33 hr Grading policy: Lecture Exam I 15 points Lecture Exam II 15 points Lecture Exam III 15 points Lecture Exam IV 15 points Presentation 15 points Lab notebook 8 points Recitation practical exercise 9 points Lab quizzes 8 points Total points = Final %

Final grades: 93-100 A 90-92 A- 87-89 B+ 83-86 B 80-82 B- 77-79 C+ 70-76 C <70 F

Recommended Texts: The Mechanics of Inhaled Pharmaceutical Aerosols. An introduction Author: Warren H. Finlay Academic Press 2001

Concepts in Inhalation Toxicology; Second Edition Edited by Roger O. McClellan and Roger F. Henderson Taylor & Francis 1995

Inhalation Aerosols Physical and Biological Basis for Therapy Edited by Anthony J. Hickey Lung Biology in Health and Disease Vol. 94 Marcel Dekker, Inc. 1996

Aerosol technology, Properties, Behavior and Measurement of Airborne Particles 2nd Edition Author: William C. Hinds Wiley Interscience 1999

Aerosol Measurement: Principles, Techniques, and Applications, 2nd Edition Edited by Klaus Willeke and Paul A. Baron John Wiley and sons, Inc. 2001

Air Sampling Instruments, 9th Edition ACIGH 2001

Textbook of Military Medicine, Part I Medical Aspects of Chemical and Biological Warfare Published by Office of the Surgeon General, DA, USA Borden Institute 1997

Biodefense: Research Methodology and Animal Models Edited by James R. Swearengen 2006 CRC Press, 2006 Aerosol Biology Schedule CRC Press, 2006

Monday Lecture 1: 0830 – 1100 (2.5 hr) Introduction to aerosol biology - Historical perspective - Course Overview and Expectations - Receive Presentation Topic

Lecture 2: 1200 – 1400 (2 hr) Aerosol generation - Introduction to nebulizers - Other generation technologies

Lecture 3: 1500 – 1700 (2 hr) Aerosol Characterization Tuesday Lecture 4: 0830 – 1100 (2.5 hr) - Aerosol Sampling

Lecture 5: 1200 – 1430 (2.5 hr) - Dose Definition - Dose Calculation Wednesday Quiz # 1: 0830 – 0930 (1 hr)

Lecture 6: 1000 – 1200 (2 hr) - Physiology of airways

Laboratory # 1: 1230 – 1730 (5 hr) Thursday Lecture 7: 0830 – 1100 (2.5 hr) - Deposition, Retention and Clearance

Lecture 8: 1130 - 1330 (2 hr) - Deployment of bioagents - Aerosol behavior - Infection

Laboratory # 2: 1400 – 1730 (3.5 hr) Friday Quiz # 2: 0830 – 0930 (1 hr)

Lecture 9: 1000 – 1200 (2 hr) - Aerosol Delivery in the laboratory - Animal Exposure

Laboratory # 3: 1230 – 1730 (5 hr) Monday Lecture 10: 0830 - 1100 (2 hr) - Animal Models 1

Lecture 11: 1130 - 1330 (2 hr) - Animal Models 2 Laboratory # 4: 1400 – 1730 (3.5 hr) Tuesday Quiz # 3 0830 – 0930 (1 hr)

Lecture 12: 0830 – 1030 (2 hr) - Biosafety Aspects

Laboratory # 5: 1130 – 1730 (6 hr) Wednesday Lecture 13: 0830 – 1000 (1.5 hr) - Pharmaceutical Aerosols Lecture 14: 1100 – 1200 (2 hr) - Communicable Aerosols

Laboratory # 6: 1300 – 1730 (4.5 hr) Thursday Quiz # 4 0830 – 0930 (1 hr)

Lecture 15: 1000 - 1130 (1.5 hr) - Emerging Technologies

Laboratory # 7: 1200 – 1730 (5.5 hr) - Practical Exercise Friday Lecture 16: 0900 - 1200 1300 – 1700 (7 hr) - Student Presentations