Nucleic Acid Isolation and Purification Manual
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Nucleic Acid Isolation and Purification Manual last update April 27, 2000
This 180-page manual collects a wide variety of reliable protocols and technical tips and will enable you to quickly find the most optimal method for your specific needs.
Below is the table of contents. To select a chapter section, click on the corresponding page number. The chapters below are in Acrobat PDF format and must be viewed, searched, and printed with version 3.0 or above of the free Adobe Acrobat Reader
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Chapter 1: Introduction
Selection Chart 3 Product Information and Major Subsequent Applications 4 Overview of Nucleic Acid Purification and Isolation 6 Methods described in this Manual 7
Chapter 2: High PureTM Technology and Silica Adsorption
Overview of Silica Adsorption 10 High PureTM PCR Template Preparation Kit 13 High PureTM PCR Product Purification Kit 20 High PureTM Plasmid Isolation Kit 27 High PureTM RNA Isolation Kit 32 High PureTM Viral RNA Kit 38 High PureTM Viral Nucleic Acid Kit 42 General Troubleshooting Procedure for all High PureTM Kits 46 PCR Clean Up Kit 53 Agarose Gel DNA Extraction Kit 58
Chapter 3: Solution-based Isolation
Overview of Solution-based Isolation 64 Split SecondTM DNA Preparation Kit 66 DNA Isolation Kit for Mammalian Blood 72 Plant DNA Isolation Kit 79 TriPureTM Isolation Kit 86
Chapter 4: Affinity Purification
Chapter 4a: Kits for Genetic Research
Overview of Kits for Genetic Research 94 mRNA Capture Kit 96 mRNA Isolation Kit 101 Triple HelixTM Plasmid Insert DNA Isolation Kit 108
Chapter 4b: Kits for Isolation of mRNA for Biomedical Research
Overview of mRNA Isolation Kits for Biomedical Research 114 RNA/DNA Stabilization Reagent for blood/Bone Marrow 118 mRNA Isolation Kit for Blood/Bone Marrow 119 mRNA Isolation Kit for White Blood Cells 126
Chapter 5: Gel Filtration
Overview of Gel Filtration 134 Quick SpinTM Columns for radiolabeled DNA/RNA purification 136 Quick SpinTM Columns Linkers 6 140
Chapter 6: Premium Performance Products for PCR and RT-PCR
Introduction 146 TitanTM One Tube RT-PCR System 147 Other Options for RT-PCR and Reverse Transcription 148 ExpandTM PCR System 149 ExpandTM High Fidelity 150 ExpandTM Long Template 151 ExpandTM 20 KbPLUS 151
Chapter 7: Appendix
Tips for Handling Nucleic Acids 154 Conversion Tables and Formulas 157 Buffers and Gels for Electrophoresis 161 Fragment Sizes of DNA Molecular Weight Markers and Recommended Types of Agaroses 163 Other Useful Information 164
Chapter 8: Ordering Guide
Ordering Guide 168
Chapter 9: Index
Index 178 Disclaimers ii
Editorial Management Richard Bauer-Hofmann, Ph.D. Doris Eisel Joe Keesey, Ph.D. Balwant Patel, Ph.D. Peter Rehberger Doris Schmitt, Ph.D. Ulrich Schuch, Ph.D. Thomas Walter, Ph.D.
Art Direction and Typesetting: typoPlus Foll + Schulz GmbH, Mannheim