1. These Two Rna Viruses Do Not Have Lipid Envelopes

NAME______

VIROLOGY TEST #3, SAMPLE R. H. REEVES

THE FIRST TWENTY QUESTIONS ARE WORTH TWO POINTS EACH. THERE IS ONLY ONE CORRECT ANSWER. PLEASE CIRCLE THE CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE QUESTION NUMBER.

1. THESE TWO RNA VIRUSES DO NOT HAVE LIPID ENVELOPES A. HEPATITIS A VIRUS AND MEASLES VIRUS B. COXSACKIE A VIRUS AND INFLUENZA C VIRUS C. HUMAN CORONA VIRUS AND RUBELLA VIRUS D. POLIOVIRUS-1 AND COLORADO TICK FEVER VIRUS E. MUMPS VIRUS AND RUBELLA VIRUS

2. THIS VIRUS HAS A DOUBLE-STRANDED GENOME WHICH IS CAPPED ON ONE 5’-END. A. LASSA FEVER VIRUS B. HUMAN ROTAVIRUS C. HEPATITIS A VIRUS D. MUMPS VIRUS E. INFLUENZA A VIRUS

3. CIRCULARIZED SINGLE-STRANDED RNA IS FOUND IN THE VIRION OF A. HEPATITIS A VIRUS B. INFLUENZA A VIRUS C BUNYAMWERA VIRUS D. EASTERN EQUINE ENCEPHALITIS VIRUS E. ROUS SARCOMA VIRUS

4. THE GENE ORDER OF ROUS SARCOMA VIRUS IS A. gag env pol src B. src gag env pol C. env pol gag src D. pol gag src env E. gag pol env src

5. THE GENOMIC RNA OF POLIOVIRUS TYPE 3 A. SERVES AS A MESSENGER RNA B. HAS A 5' CAP C. BOTH OF THE ABOVE D. NEITHER OF THE ABOVE

6. THE VIRUS FAMILY THAT HAS AN AMBISENSE GENOME IS A. REOVIRIDAE B. ARENAVIRIDAE C. BOTH OF THE ABOVE D. NEITHER OF THE ABOVE MICROBIOLOGY TEST #3 PAGE 3 KEY FOR QUESTIONS 14-28. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY WORD MAY BE USED MORE THAN ONCE.

A. EASTERN EQUINE ENCEPHALITIS VIRUS B. RHINOVIRUS TYPE 23 C. MEASLES (RUBEOLA) VIRUS D. ROTAVIRUS E. LASSA FEVER VIRUS F. INFLUENZA A VIRUS G. ROUS SARCOMA VIRUS I. RABIES VIRUS

_____14. ITS M2 PROTEIN INTERACTS WITH AMANTIDINE

_____15. ITS CLOSE RELATIVE IS THE TARGET OF THE SALK VACCINE

_____16. ITS ONCOGENE IS A PROTEIN KINASE

_____17. THE CURRENT PEDIATRIC VACCINE FOR THIS VIRUS USES AN ATTENUATED VIRUS

_____18. THE MAJOR CAUSE OF INFANTILE GASTROENTERITIS

_____19. ESTABLISHES “LATENCY” IN NERVOUS TISSUE (SSPE)

_____20. HORSES AND MAN ARE “DEAD END” HOSTS

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21. EXPLAIN WHY THE CELLULAR ONCOGENES (C-0NCs) THAT HAVE BEEN CHARACTERIZED ALWAYS HAVE INTRONS, WHEREAS THE ONCOGENES THAT HAVE BEEN FOUND IN RNA TUMOR (SARCOMA) VIRUSES (V-ONCs) HAVE ANALOGOUS CODING SEQUENCES BUT NEVER HAVE INTRONS.

22. THE MONONEGAVIRALES VIRUSES MAKE THEIR mRNAs BY A “STUTTERING” MECHANISM. EXPLAIN.

23. THE REPLICATION OF INFLUENZA VIRUS IS EFFECTIVELY INHIBITED BY THE DRUG ALPHA-AMINITIN, WHICH STRONGLY INHIBITS THE HOST ENZYME RNA POLYMERASE II. THIS DRUG, HOWEVER, HAS LITTLE EFFECT ON THE REPLICATION OF OTHER RNA VIRUSES. EXPLAIN.