Hptlc Method Development of Extract Of

“HPTLC METHOD DEVELOPMENT OF EXTRACT OF LEAVES OF CASSIA SOPHERA LINN.”

M.PHARM DISSERTATION PROTOCOL

Submitted to

Rajiv Gandhi University of Health Sciences Bangalore, Karnataka.

Miss. KIBILE MAHESHWARI RAGHUNATH B. Pharm

Under the Guidance of Dr. KISHORESINGH. CHATRAPATI M.Pharm., Ph. D

DEPARTMENT OF PHARMACEUTICAL CHEMISTRY R.M.E.S’S COLLEGE OF PHARMACY GULBARGA-585102 2012-2013 RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, BANGALORE, KARNATAKA

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. NAME OF THE MISS. KIBILE MAHESHWARI RAGHUNATH CANDIDATE AND C/O- GURUDATTA SILVER MARCHANT, ADDRESS PANDIT COMPLEX, SARAF BAZAR, GULBARGA- 585102

2. NAME OF THE R.M.E.S’S COLLEGE OF PHARMACY, INSTITUTION GULBARGA-585102.

3. COURSE OF STUDY MASTER OF PHARMACY (PHARMACEUTICAL AND SUBJECT CHEMISTRY) 4. DATE OF ADMISSION 29th Octomber 2011. TO COURSE

5. TITLE OF THE TOPIC:

“HPTLC METHOD DEVELOPMENT OF EXTRACT OF LEAVES OF CASSIA SOPHERA LINN.”

- 2 - - 3 - BRIEF RESUME OF THE INTENDED WORK:

6.1 INTRODUCTION:

Cassia sophera Linn (Fam. Caesalpiniaceae) is locally known as Kasodia and grows abundantly in India, most of the tropical countries as well as in the plain land, hilly areas of Chittagong Hill Tracts, Sylhet and throughout Bangladesh. Cassia sophera Linn. is a shrub or under shrub (2.9-3.0 m) annual or perennial and it is an important medicinal plant and considered to have expectorant properties. The bark in the form of infusion and the powdered seeds, mixed with honey, are given in diabetes. The bark, leaves and seeds are used as a cathartic and the juice of the leaves is specific for ringworm, especially when made into a plaster in combination with sandal-wood. The root is administered internally with black pepper for snake bite1. The leaves are used in asthma2, bronchitis. Infusion of leaves is also useful in gonorrhea and syphilitic sores. In ethno botanical literature it is mentioned to be effective in the treatment of pityriasis, psoriasis, asthma2, acute bronchitis, cough, anthelmintic and diabetes. The chemical analysis of the seed of Cassia sophera Linn. reveals the presence of ascorbic acid, dehydroascorbic acid, quercetine and β-sistosterol, but no scientific study is reported on the varietal level of plant3. As per literature, no study has been reported on phytoconstituents of Cassia sophera. Therefore, in the present an attempt has been made to study HPTLC method development and validation of leaves of Cassia sophera Linn.

6.2 NEED FOR THE STUDY: Many species of medicinal plant have been shown to produce beneficial effects in the treatment of various diseases. Historically, plants have been used as folk medicines against various types of diseases. Even today crude extract of medicinal plants are used widely by country to cure human ailments in many countries. Cassia sophera Linn. plant mainly found in Bangladesh and Indian sub continent, which is widely used as folk medicine for the treatment of many diseases1. According to the physicians of Unani medicine, three species of plants viz., Cassia occidentalis Linn. and Cassia sophera Linn. var. Purpurea Roxb. are the varieties of ‘Kasondi’ and are invariably conditions. ‘Kasondi’ is described in Unani literature for various conditions

- 4 - - 5 - 9. Signature of Candidate 10. Remark of the guide The above information and literature has been extensively investigated, verified and was found to be correct. The present study will be carried out under my supervision and guidance. 11. Name and Designation of (in block letters) DR. KISHORE SINGH CHATRAPATI.

11.1 Guide M. Pharm. PhD PROFESSOR, Department of Pharmaceutical chemistry, R.M.E.S’S College of Pharmacy, Gulbarga-585 102

11.2 Signature 11.3 Co-guide (if any) Mr.HANAMANTH.J.KALLUR

M. Pharm. Lecturer, Department of pharmaceutical chemistry 11.4 Signature 11.5 Head of the Department DR. KISHORE SINGH CHATRAPATI.

M. Pharm. PhD PROFESSOR & HOD, Department of Pharmaceutical chemistry, R.M.E.S’S College of Pharmacy, Gulbarga-585 102

11.6 Signature 12. 12.1 Remarks of the The above mentioned information is correct and I Chairman & Principal recommend the same for approval.

12.2 Signature PROF. HARIPRASSANNA R.C. M. Pharm.(PhD) PRINCIPAL, R.M.E.S’S College of Pharmacy, Gulbarga-585 102